LOCUS       NM_005884               2830 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens p21 (RAC1) activated kinase 4 (PAK4), transcript
            variant 1, mRNA.
ACCESSION   NM_005884
VERSION     NM_005884.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Zhang,J., Song,Y., Ahn,A.R., Park,H.S., Park,S.H., Moon,Y.J.,
            Kim,K.M. and Jang,K.Y.
  TITLE     PAK4 Is Involved in the Stabilization of PD-L1 and the Resistance
            to Doxorubicin in Osteosarcoma and Predicts the Survival of
            Diagnosed Patients
  JOURNAL   Cells 13 (17), 1444 (2024)
   PUBMED   39273017
  REMARK    GeneRIF: PAK4 Is Involved in the Stabilization of PD-L1 and the
            Resistance to Doxorubicin in Osteosarcoma and Predicts the Survival
            of Diagnosed Patients.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wu,D., Yu,H.C., Cha,H.N., Park,S., Lee,Y., Yoon,S.J., Park,S.Y.,
            Park,B.H. and Bae,E.J.
  TITLE     PAK4 phosphorylates and inhibits AMPKalpha to control glucose
            uptake
  JOURNAL   Nat Commun 15 (1), 6858 (2024)
   PUBMED   39127697
  REMARK    GeneRIF: PAK4 phosphorylates and inhibits AMPKalpha to control
            glucose uptake.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2830)
  AUTHORS   Chi,X.J., Song,Y.B., Zhang,H., Wei,L.Q., Gao,Y., Miao,X.J.,
            Yang,S.T., Lin,C.Y., Lan,D. and Zhang,X.
  TITLE     TBC1D10B promotes tumor progression in colon cancer via
            PAK4-mediated promotion of the PI3K/AKT/mTOR pathway
  JOURNAL   Apoptosis 29 (7-8), 1185-1197 (2024)
   PUBMED   38824479
  REMARK    GeneRIF: TBC1D10B promotes tumor progression in colon cancer via
            PAK4-mediated promotion of the PI3K/AKT/mTOR pathway.
REFERENCE   4  (bases 1 to 2830)
  AUTHORS   Tang,L., Ye,H., Chen,L., Dong,W., Hu,X. and Yu,L.
  TITLE     Tumorigenic role of Pak4 in ovarian cancer and its correlation with
            immune infiltration
  JOURNAL   BMC Med Genomics 17 (1), 148 (2024)
   PUBMED   38807162
  REMARK    GeneRIF: Tumorigenic role of Pak4 in ovarian cancer and its
            correlation with immune infiltration.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2830)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   6  (bases 1 to 2830)
  AUTHORS   Lu,Y., Pan,Z.Z., Devaux,Y. and Ray,P.
  TITLE     p21-activated protein kinase 4 (PAK4) interacts with the
            keratinocyte growth factor receptor and participates in
            keratinocyte growth factor-mediated inhibition of oxidant-induced
            cell death
  JOURNAL   J Biol Chem 278 (12), 10374-10380 (2003)
   PUBMED   12529371
  REMARK    GeneRIF: PAK4 interacts with KGF receptor and mediate
            anti-apoptosis effects of KGF on epithelial cells.
REFERENCE   7  (bases 1 to 2830)
  AUTHORS   Zhang,H., Li,Z., Viklund,E.K. and Stromblad,S.
  TITLE     P21-activated kinase 4 interacts with integrin alpha v beta 5 and
            regulates alpha v beta 5-mediated cell migration
  JOURNAL   J Cell Biol 158 (7), 1287-1297 (2002)
   PUBMED   12356872
REFERENCE   8  (bases 1 to 2830)
  AUTHORS   Dan,C., Kelly,A., Bernard,O. and Minden,A.
  TITLE     Cytoskeletal changes regulated by the PAK4 serine/threonine kinase
            are mediated by LIM kinase 1 and cofilin
  JOURNAL   J Biol Chem 276 (34), 32115-32121 (2001)
   PUBMED   11413130
REFERENCE   9  (bases 1 to 2830)
  AUTHORS   Bagrodia,S. and Cerione,R.A.
  TITLE     Pak to the future
  JOURNAL   Trends Cell Biol 9 (9), 350-355 (1999)
   PUBMED   10461188
  REMARK    Review article
REFERENCE   10 (bases 1 to 2830)
  AUTHORS   Abo,A., Qu,J., Cammarano,M.S., Dan,C., Fritsch,A., Baud,V.,
            Belisle,B. and Minden,A.
  TITLE     PAK4, a novel effector for Cdc42Hs, is implicated in the
            reorganization of the actin cytoskeleton and in the formation of
            filopodia
  JOURNAL   EMBO J 17 (22), 6527-6540 (1998)
   PUBMED   9822598
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG773480.1, AF005046.1 and
            AB032968.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 27, 2021 this sequence version replaced NM_005884.4.
            
            Summary: PAK proteins, a family of serine/threonine p21-activating
            kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are
            critical effectors that link Rho GTPases to cytoskeleton
            reorganization and nuclear signaling. They serve as targets for the
            small GTP binding proteins Cdc42 and Rac and have been implicated
            in a wide range of biological activities. PAK4 interacts
            specifically with the GTP-bound form of Cdc42Hs and weakly
            activates the JNK family of MAP kinases. PAK4 is a mediator of
            filopodia formation and may play a role in the reorganization of
            the actin cytoskeleton. Multiple alternatively spliced transcript
            variants encoding distinct isoforms have been found for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853563.16957.1, AF005046.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360442.8/ ENSP00000353625.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                BG773480.1         32-67
            37-2398             AF005046.1         18-2379
            2399-2830           AB032968.1         1892-2323
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.2"
     gene            1..2830
                     /gene="PAK4"
                     /note="p21 (RAC1) activated kinase 4"
                     /db_xref="GeneID:10298"
                     /db_xref="HGNC:HGNC:16059"
                     /db_xref="MIM:605451"
     exon            1..134
                     /gene="PAK4"
                     /inference="alignment:Splign:2.1.0"
     exon            135..207
                     /gene="PAK4"
                     /inference="alignment:Splign:2.1.0"
     exon            208..433
                     /gene="PAK4"
                     /inference="alignment:Splign:2.1.0"
     CDS             230..2005
                     /gene="PAK4"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein kinase related to S. cerevisiae STE20, effector
                     for Cdc42Hs; p21(CDKN1A)-activated kinase 4;
                     serine/threonine-protein kinase PAK 4; p21 protein
                     (Cdc42/Rac)-activated kinase 4"
                     /codon_start=1
                     /product="serine/threonine-protein kinase PAK 4 isoform 1"
                     /protein_id="NP_005875.1"
                     /db_xref="CCDS:CCDS12528.1"
                     /db_xref="GeneID:10298"
                     /db_xref="HGNC:HGNC:16059"
                     /db_xref="MIM:605451"
                     /translation="MFGKRKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIE
                     ESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRR
                     DSPPPPARARQENGMPEEPATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRP
                     KSSREGSGGPQESSRDKRPLSGPDVGTPQPAGLASGAKLAAGRPFNTYPRADTDHPSR
                     GAQGEPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPAC
                     TPAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGI
                     VCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELW
                     VVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTH
                     DGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEM
                     VDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAEL
                     LKHPFLAKAGPPASIVPLMRQNRTR"
     exon            434..892
                     /gene="PAK4"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1327
                     /gene="PAK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1461
                     /gene="PAK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1588
                     /gene="PAK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1714
                     /gene="PAK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..1849
                     /gene="PAK4"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..2830
                     /gene="PAK4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2809..2814
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAK4"
                     /note="hexamer: ATTAAA"
     polyA_site      2830
                     /gene="PAK4"
                     /note="major polyA site"
ORIGIN      
        1 ggatgttcgt tggggattca acatggcggc gggagtgtcc gcggtggtgg cggtgcaaga
       61 gagctgaggg aggcgcgagg gcgcggagtt ccaggtcgag cagttaggcc gcgagcgact
      121 gcggcgccga gccgatgagt aacccgaagc ccctagagga gtggtcacct gcctgagggc
      181 acttctgtcc caccagcatc agaccaggcc gcaccgagtc cccggcacca tgtttgggaa
      241 gaggaagaag cgggtggaga tctccgcgcc gtccaacttc gagcaccgcg tgcacacggg
      301 cttcgaccag cacgagcaga agttcacggg gctgccccgc cagtggcaga gcctgatcga
      361 ggagtcggct cgccggccca agcccctcgt cgaccccgcc tgcatcacct ccatccagcc
      421 cggggccccc aagaccatcg tgcggggcag caaaggtgcc aaagatgggg ccctcacgct
      481 gctgctggac gagtttgaga acatgtcggt gacacgctcc aactccctgc ggagagacag
      541 cccgccgccg cccgcccgtg cccgccagga aaatgggatg ccagaggagc cggccaccac
      601 ggccagaggg ggcccaggga aggcaggcag ccgaggccgg ttcgccggtc acagcgaggc
      661 gggtggcggc agtggtgaca ggcgacgggc ggggccagag aagaggccca agtcttccag
      721 ggagggctca gggggtcccc aggagtcctc ccgggacaaa cgccccctct ccgggcctga
      781 tgtcggcacc ccccagcctg ctggtctggc cagtggggcg aaactggcag ctggccggcc
      841 ctttaacacc tacccgaggg ctgacacgga ccacccatcc cggggtgccc agggggagcc
      901 tcatgacgtg gcccctaacg ggccatcagc ggggggcctg gccatccccc agtcctcctc
      961 ctcctcctcc cggcctccca cccgagcccg aggtgccccc agccctggag tgctgggacc
     1021 ccacgcctca gagccccagc tggcccctcc agcctgcacc cccgccgccc ctgctgttcc
     1081 tgggccccct ggcccccgct caccacagcg ggagccacag cgagtatccc atgagcagtt
     1141 ccgggctgcc ctgcagctgg tggtggaccc aggcgacccc cgctcctacc tggacaactt
     1201 catcaagatt ggcgagggct ccacgggcat cgtgtgcatc gccaccgtgc gcagctcggg
     1261 caagctggtg gccgtcaaga agatggacct gcgcaagcag cagaggcgcg agctgctctt
     1321 caacgaggtg gtaatcatga gggactacca gcacgagaat gtggtggaga tgtacaacag
     1381 ctacctggtg ggggacgagc tctgggtggt catggagttc ctggaaggag gcgccctcac
     1441 cgacatcgtc acccacacca ggatgaacga ggagcagatc gcggccgtgt gccttgcagt
     1501 gctgcaggcc ctgtcggtgc tccacgccca gggcgtcatc caccgggaca tcaagagcga
     1561 ctcgatcctg ctgacccatg atggcagggt gaagctgtca gactttgggt tctgcgccca
     1621 ggtgagcaag gaagtgcccc gaaggaagtc gctggtcggc acgccctact ggatggcccc
     1681 agagctcatc tcccgccttc cctacgggcc agaggtagac atctggtcgc tggggataat
     1741 ggtgattgag atggtggacg gagagccccc ctacttcaac gagccacccc tcaaagccat
     1801 gaagatgatt cgggacaacc tgccaccccg actgaagaac ctgcacaagg tgtcgccatc
     1861 cctgaagggc ttcctggacc gcctgctggt gcgagaccct gcccagcggg ccacggcagc
     1921 cgagctgctg aagcacccat tcctggccaa ggcagggccg cctgccagca tcgtgcccct
     1981 catgcgccag aaccgcacca gatgaggccc agcgcccttc ccctcaacca aagagccccc
     2041 cgggtcaccc ccgccccact gaggccagta gggggccagg cctcccactc ctcccagccc
     2101 gggagatgct ccgcgtggca ccaccctcct tgctgggggt agatgagacc ctactactga
     2161 actccagttt tgatctcgtg acttttagaa aaacacaggg actcgtggga gcaagcgagg
     2221 ctcccaggac ccccaccctc tgggacaggc cctcccccat gttcttctgt ctccaggaag
     2281 ggcagcggcc ctcccatcac tggaagtctg cagtgggggt cgctgggggt ggagagaaca
     2341 ctaagaggtg aacatgtatg agtgtgtgca cgcgtgtgag tgtgcatgtg tgtgtgtgca
     2401 aaggtccagc caccccgtcc tccagcctgc aaggggtgtc tggcgccttg cctgacaccc
     2461 agccccctct ccccctgagc cattgtgggg gtcgatcatg aatgtccgaa gagtggcctt
     2521 ttcccgtagc cctgcgcccc ctttctgtgg ctggatgggg agacaggtca gggcccccca
     2581 ccctctccag cccctgcagc aaatgactac tgcacctgga cagcctcctc ttttctagaa
     2641 gtctatttat attgtcattt tataacactc tagcccctgc ccttattggg ggacagatgg
     2701 tccctgtcct gcggggtggc cctggcagaa ccactgcctg aagaaccagg ttcctgcccg
     2761 gtcagcgcag ccccagcccg cccacccctg cctcgagtta gttttacaat taaaacattg
     2821 tcttgttttg
//
