LOCUS       NM_002578               9126 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens p21 (RAC1) activated kinase 3 (PAK3), transcript
            variant 2, mRNA.
ACCESSION   NM_002578 XM_001714511 XM_001718168
VERSION     NM_002578.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Chen,X., Ruiz-Velasco,A., Zou,Z., Hille,S.S., Ross,C., Fonseka,O.,
            Gare,S.R., Alatawi,N.H.O., Raja,R., Zhang,J., Kaur,N., Zhao,X.,
            Morrell-Davies,H., Miller,J.M., Abouleisa,R.R.E., Ou,Q., Frank,D.,
            Rutter,M.K., Pinali,C., Wang,T., Mohamed,T.M.A., Muller,O.J. and
            Liu,W.
  TITLE     PAK3 Exacerbates Cardiac Lipotoxicity via SREBP1c in Obesity
            Cardiomyopathy
  JOURNAL   Diabetes 73 (11), 1805-1820 (2024)
   PUBMED   39137120
  REMARK    GeneRIF: PAK3 Exacerbates Cardiac Lipotoxicity via SREBP1c in
            Obesity Cardiomyopathy.
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   Kervarrec,T., Westphal,D., Pissaloux,D., Legrand,M., Tirode,F.,
            Neuhart,A., Drouot,F., Becker,J.C., Macagno,N., Seris,A.,
            Jouary,T., Beltzung,F., Jullie,M.L., Harms,P.W., Cribier,B.,
            Mourah,S., Jouenne,F., Fromont,G., Louveau,B., Mancini,M.,
            Kazakov,D.V., de la Fouchardiere,A. and Battistella,M.
  TITLE     Porocarcinomas with PAK1/2/3 fusions: a series of 12 cases
  JOURNAL   Histopathology 85 (4), 566-578 (2024)
   PUBMED   38785043
  REMARK    GeneRIF: Porocarcinomas with PAK1/2/3 fusions: a series of 12
            cases.
REFERENCE   3  (bases 1 to 9126)
  AUTHORS   Magne,N., Rousseau,V., Duarte,K., Poea-Guyon,S., Gleize,V.,
            Mutel,A., Schmitt,C., Castel,H., Idbaih,A., Huillard,E., Sanson,M.
            and Barnier,J.V.
  TITLE     PAK3 is a key signature gene of the glioma proneural subtype and
            affects its proliferation, differentiation and growth
  JOURNAL   Cell Oncol (Dordr) 44 (6), 1257-1271 (2021)
   PUBMED   34550532
  REMARK    GeneRIF: PAK3 is a key signature gene of the glioma proneural
            subtype and affects its proliferation, differentiation and growth.
REFERENCE   4  (bases 1 to 9126)
  AUTHORS   Tan,X., Tong,L., Li,L., Xu,J., Xie,S., Ji,L., Fu,J., Liu,Q.,
            Shen,S., Liu,Y., Xiao,Y., Gao,F., Moses,R.E., Bardeesy,N., Wang,Y.,
            Zhang,J., Tang,L., Li,L., Wong,K.K., Song,D., Yang,X., Liu,J. and
            Li,X.
  TITLE     Loss of Smad4 promotes aggressive lung cancer metastasis by
            de-repression of PAK3 via miRNA regulation
  JOURNAL   Nat Commun 12 (1), 4853 (2021)
   PUBMED   34381046
  REMARK    GeneRIF: Loss of Smad4 promotes aggressive lung cancer metastasis
            by de-repression of PAK3 via miRNA regulation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9126)
  AUTHORS   Kreis,P., Rousseau,V., Thevenot,E., Combeau,G. and Barnier,J.V.
  TITLE     The four mammalian splice variants encoded by the p21-activated
            kinase 3 gene have different biological properties
  JOURNAL   J Neurochem 106 (3), 1184-1197 (2008)
   PUBMED   18507705
REFERENCE   6  (bases 1 to 9126)
  AUTHORS   Rousseau,V., Goupille,O., Morin,N. and Barnier,J.V.
  TITLE     A new constitutively active brain PAK3 isoform displays modified
            specificities toward Rac and Cdc42 GTPases
  JOURNAL   J Biol Chem 278 (6), 3912-3920 (2003)
   PUBMED   12464619
  REMARK    GeneRIF: PAK3 codes for a splice variant that contains a 45-bp
            alternatively spliced exon. The exon encodes 15 aa that are
            inserted in the regulatory domain. PAK3b displays a high kinase
            activity in starved cells that is not further stimulated by active
            GTPases.
REFERENCE   7  (bases 1 to 9126)
  AUTHORS   Allen,K.M., Gleeson,J.G., Bagrodia,S., Partington,M.W.,
            MacMillan,J.C., Cerione,R.A., Mulley,J.C. and Walsh,C.A.
  TITLE     PAK3 mutation in nonsyndromic X-linked mental retardation
  JOURNAL   Nat Genet 20 (1), 25-30 (1998)
   PUBMED   9731525
REFERENCE   8  (bases 1 to 9126)
  AUTHORS   Illarioshkin,S.N., Tanaka,H., Markova,E.D., Nikolskaya,N.N.,
            Ivanova-Smolenskaya,I.A. and Tsuji,S.
  TITLE     X-linked nonprogressive congenital cerebellar hypoplasia: clinical
            description and mapping to chromosome Xq
  JOURNAL   Ann Neurol 40 (1), 75-83 (1996)
   PUBMED   8687195
REFERENCE   9  (bases 1 to 9126)
  AUTHORS   Manser,E., Chong,C., Zhao,Z.S., Leung,T., Michael,G., Hall,C. and
            Lim,L.
  TITLE     Molecular cloning of a new member of the p21-Cdc42/Rac-activated
            kinase (PAK) family
  JOURNAL   J Biol Chem 270 (42), 25070-25078 (1995)
   PUBMED   7559638
REFERENCE   10 (bases 1 to 9126)
  AUTHORS   Manser,E., Leung,T., Salihuddin,H., Zhao,Z.S. and Lim,L.
  TITLE     A brain serine/threonine protein kinase activated by Cdc42 and Rac1
  JOURNAL   Nature 367 (6458), 40-46 (1994)
   PUBMED   8107774
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA957724.1, DA022842.1,
            AK290504.1, BC117353.1, DB563607.2 and AL031117.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_002578.4.
            
            Summary: The protein encoded by this gene is a serine-threonine
            kinase and forms an activated complex with GTP-bound RAS-like
            (P21), CDC2 and RAC1. This protein may be necessary for dendritic
            development and for the rapid cytoskeletal reorganization in
            dendritic spines associated with synaptic plasticity. Defects in
            this gene are the cause of a non-syndromic form of X-linked
            intellectual disability. Alternatively spliced transcript variants
            encoding different isoforms have been identified. [provided by
            RefSeq, Jul 2017].
            
            Transcript Variant: This variant (2) represents use of an alternate
            promoter compared to variant 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.35228.1,
                                           SRR1803612.138966.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372007.10/ ENSP00000361077.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                DA957724.1         45-66
            23-550              DA022842.1         1-528
            551-2213            AK290504.1         389-2051
            2214-2312           BC117353.1         1731-1829
            2313-2451           AK290504.1         2151-2289
            2452-2710           DB563607.2         447-705
            2711-9126           AL031117.1         7355-13770
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq23"
     gene            1..9126
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /note="p21 (RAC1) activated kinase 3"
                     /db_xref="GeneID:5063"
                     /db_xref="HGNC:HGNC:8592"
                     /db_xref="MIM:300142"
     exon            1..225
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            226..292
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            293..402
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            403..550
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            551..752
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     CDS             578..2212
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /EC_number="2.7.11.1"
                     /note="isoform a is encoded by transcript variant 2; p21
                     (CDKN1A)-activated kinase 3; oligophrenin-3;
                     serine/threonine-protein kinase PAK 3; adriamycin
                     resistance-associated; p21 protein (Cdc42/Rac)-activated
                     kinase 3"
                     /codon_start=1
                     /product="serine/threonine-protein kinase PAK 3 isoform a"
                     /protein_id="NP_002569.1"
                     /db_xref="CCDS:CCDS14554.1"
                     /db_xref="GeneID:5063"
                     /db_xref="HGNC:HGNC:8592"
                     /db_xref="MIM:300142"
                     /translation="MSDGLDNEEKPPAPPLRMNSNNRDSSALNHSSKPLPMAPEEKNK
                     KARLRSIFPGGGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLL
                     QTSNITKLEQKKNPQAVLDVLKFYDSKETVNNQKYMSFTSGDKSAHGYIAAHPSSTKT
                     ASEPPLAPPVSEEEDEEEEEEEDENEPPPVIAPRPEHTKSIYTRSVVESIASPAVPNK
                     EVTPPSAENANSSTLYRNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQ
                     GASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLV
                     GDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDN
                     ILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGI
                     MAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRG
                     SAKELLQHPFLKLAKPLSSLTPLIIAAKEAIKNSSR"
     exon            753..853
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            854..1007
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1045
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1177
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1343
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1407
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1456
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1569
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1687
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1787
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            1788..1984
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2122
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..9126
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9103..9108
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /note="hexamer: AATAAA"
     polyA_site      9126
                     /gene="PAK3"
                     /gene_synonym="ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3;
                     PAK-3; PAK3beta; XLID30"
                     /note="major polyA site"
ORIGIN      
        1 accgcgggcg ggcagctgtg ccagctaacc gtctgggatc tcgcactggg ggctgcagct
       61 tttccccgcc tcgagccagt gtgcgggggc gggagaagag ccagggggag cgggctgggc
      121 ccggggctgc ggctgcggcc gcggggctgc ggctccccag ccccgccagc tggagcgctc
      181 ggaggtagag gaaaggtctt gacggggtgg ctggatccgt ggcagaatcc agttccagat
      241 tctagacttg agggttctgg gctgttggtc tgtagaagcg aaggagagaa ggactcaaat
      301 ccaggccaag tgtatggctg tctgaggtat tggaacagaa ggaggtccat tcctgttggt
      361 gacaacaccg tggccctgtt ctgggatgag caaggtgtaa aggtttcccc caagaaagag
      421 cagctgagtc cttgcatctt gtggcagctg gtgtgcccag cactgagtct gtaggagctg
      481 aagccagccc ggacccttct catgggcagt gcccacctgt gctgaagtcc tgcagcggtg
      541 gcggtgtgag gagctgtgaa attagttgta actgaaaatg tctgacggtc tggataatga
      601 agagaaaccc ccggctcctc cactgaggat gaatagtaac aaccgggatt cttcagcact
      661 caaccacagc tccaaaccac ttcccatggc ccctgaagag aagaataaga aagccaggct
      721 tcgctctatc ttcccaggag gaggggataa aaccaataag aagaaggaga aagagcgccc
      781 agagatctct cttccttcag actttgagca tacgattcat gtggggtttg atgcagtcac
      841 cggggaattc actggaattc cagagcaatg ggcacgatta ctccaaactt ccaacataac
      901 aaaattggaa cagaagaaga acccacaagc tgttctagat gttctcaaat tctatgattc
      961 caaagaaaca gtcaacaacc agaaatacat gagctttaca tcaggagata aaagtgcaca
     1021 tggatacata gcagcccatc cttcgagtac aaaaacagca tctgagcctc cattggcccc
     1081 tcctgtgtct gaagaagaag atgaagagga agaagaagaa gaagatgaaa atgagccacc
     1141 accagttatc gcaccaagac cagagcatac aaaatcaatc tatactcgtt ctgtggttga
     1201 atccattgct tcaccagcag taccaaataa agaggtcaca ccaccctctg ctgaaaatgc
     1261 caattccagt actttgtaca ggaacacaga tcggcaaaga aaaaaatcca agatgacaga
     1321 tgaggagatc ttagagaagc taagaagcat tgtgagtgtt ggggacccaa agaaaaaata
     1381 cacaagattt gaaaaaattg gtcaaggggc atcaggtact gtttatacag cactagacat
     1441 tgcaacagga caagaggtgg ccataaagca gatgaacctt caacagcaac ccaagaagga
     1501 attaattatt aatgaaattc tggtcatgag ggaaaataag aaccctaata ttgttaatta
     1561 tttagatagc tacttggtgg gtgatgaact atgggtagtc atggaatact tggctggtgg
     1621 ctctctgact gatgtggtca cagagacctg tatggatgaa ggacagatag cagctgtctg
     1681 cagagagtgc ctgcaagctt tggatttcct gcactcaaac caggtgatcc atagagatat
     1741 aaagagtgac aatattcttc tcgggatgga tggctctgtt aaattgactg actttgggtt
     1801 ctgtgcccag atcactcctg agcaaagtaa acgaagcact atggtgggaa ccccatattg
     1861 gatggcacct gaggtggtga ctcgaaaagc ttatggtccg aaagttgata tctggtctct
     1921 tggaattatg gcaattgaaa tggtggaagg tgaaccccct taccttaatg aaaatccact
     1981 cagggcattg tatctgatag ccactaatgg aactccagag ctccagaatc ctgagagact
     2041 gtcagctgta ttccgtgact ttttaaatcg ctgtcttgag atggatgtgg ataggcgagg
     2101 atctgccaag gagcttttgc agcatccatt tttaaaatta gccaagcctc tctccagcct
     2161 gactcctctg attatcgctg caaaggaagc aattaagaac agcagccgct aagactgcaa
     2221 gccttacacc tcaccatctc cctcatgagt aagactgaaa taaaactctg ctgcaggaaa
     2281 gatggaagaa aagacagtca aatggggtgg gggttcttta cctttcaaat gaatagaaac
     2341 ttcttataag cctttttcct actccctcag attatgtaat ttatttgtaa gcctgaatcg
     2401 cagcccaaac agggcagcaa tgttgaagtg accataaagt ggtcacttcc accgtgaagc
     2461 gaaagagcca gtagtgaatc ccctcatttt gtgcattcac tttgaagaaa aaggtttctc
     2521 aaagatgcac actccctctt catagtgttg tgtttgtttt taagttagag agtagtccct
     2581 cttgcattca aacctccttc aaaactcctt acccaatgtg atgtttttca cttgcattgt
     2641 cattagatgt ccagaaaaaa aaaagatgtc aaaatgtttt tctaaaaaaa gaaagcaaaa
     2701 aaagcaaggc aaaaaaaaaa aaaaaaacaa acaaaaacaa aaacaaaaca aaaacaagca
     2761 aacaaaaaat accagagcaa gtactgtgtg aacatgtgga agtccatgcc ctaatagagt
     2821 tgcaattttt tattcttctt ctatagtggt ggcttggttt gtgtacctat ttttctgcat
     2881 ttgtattgga aaaggtttct tttaagacat tttccaaaag tggagaggaa tatgtgtgtt
     2941 caggaagggc tttcaaaaaa ctgtatatct aaataaagct caaacggtga aatcctgtca
     3001 cattttcaca atgatgctta aaagataatt gagtaaacca ggttgttaat ctccttaata
     3061 cctgaaagag gacacactga aactgaaact gtgacatcct gctaggtgag ttcaggttct
     3121 gaacctagga aatcctcata ggagaaacca catttaaaca aagatgggac tttctctgag
     3181 agccaaaacc agataaatgt agaatactga aatccttgtt ggacattaag taaacaaaga
     3241 taatgatacc taaattaatc ctctcttgtg cttatgaaac atatgcactg taaaataggc
     3301 ataccaggag gaaatagata cattaatcat catttactta tgatacaaat tatttatttt
     3361 gacaatttat aacgtttaaa aaagtttttt aaagatctag agaaaggtga tatagtaaac
     3421 attcaactct gtaagaaatg ggaggtcagt gaaggctaca tcccaatcaa tatttggctc
     3481 taagtacctc ttcccatttt tcctatgtat cacctatttc tgtttcggaa tatggtgtgt
     3541 tcatgcttag ttctttgggc ttttgaatat caaaagcata ttcataaatg tcttgaaatt
     3601 ctctccagtg gaaaataatt ttaacttaca atcatatccc aagaaatgtc agtccgacag
     3661 aattccttat atgacttggg gaaaataaca aaatttgact actatttcac catatatcta
     3721 tttattaaaa aattcaacag ttggcacttc ctgaatcttc tgagagtaga aaaatatctg
     3781 cggagtgtct gtgtagaaaa ggatatgcct ctcttttgag tgtattgaca attttgtaaa
     3841 ttacagaaag ttgtttctct aagcctttga aaaactaaca atttgtgtta tagaaggctt
     3901 cttaatttgc agtataaaag aatctaaaca gaacttatgt acattcagcc agaaggggaa
     3961 agagatcagt tacataggcc tctctccttc tttgccaagg tacatccatc catctaacca
     4021 tccatatatc cacatcttaa aatgaaagca ctttctttag agtttcagca aactatatag
     4081 tgtacgtgtt tatgttcagg agatgacccc actggtgtat ttcctatttt ccctattgtt
     4141 ttctttgact gtaaaagttg ggagaggctt gacctcctcc ccttgaaaat gtccacagtg
     4201 ggataaaaca acaaatgtga aaagaaaatg aaacggtaat attaatttga agcatactat
     4261 gttatacttt gcaaaaacga atctgggcct gtaattttta atgccacact gctctaatga
     4321 gagagagagg ccttaatttt gatttcattt aaaaataagt actttaaaaa atttttcact
     4381 catagtgccg ggaaattcaa tgaaatcctg ggatgcaaat aaaaatcagt acattagtga
     4441 ctgtgtcctg ccagtggaga gagcccaata cctggttagg aagccctatt cattagttag
     4501 catcccttac atgttgagaa ggcctttttt ttgttgttat tttggagacc ttggagcagt
     4561 gacccttcag atcactgtag gcagagaaat ggcttctctc ttatgctttc agttcagcat
     4621 attaacaatg aggagccagg tacttcttta ctaccacttt gtaccaagat ttgataataa
     4681 tatatcccag gaggcattac ttttataaat ttgtattcat gtaaattttc aaatgagaac
     4741 agcttctaaa gccccttccc tgtattggag agttatgtat atttctaata agtattagaa
     4801 agaagctgtt tctcatgcca cagtgatgct gaaggattca catttggtac aatcgagtaa
     4861 cttgaacgcc agattgttaa cagtttattc tctttccctg gatttttaag ctcatcttga
     4921 cacaggtgag tctatccaaa tctttgatgt tgctagtgtg ccctgagata acgagggcac
     4981 atctttcaat gttgattcca aaatgtcctg agttaggaat agggcagtgg gaaagtcagg
     5041 gaagggtgag aagcacagta gagattattt atttaaaaaa ggaaagaacg ttaatgttgt
     5101 tagcaaggat ccagtgcgtt gtcataatcc catgaggatt ttcagatgac acaatcccct
     5161 caaatcagtc accatgttgg gtaatgactt cgttcttgct gatctcgtgt gtgtgtcatt
     5221 gtaaatattt gtgtgtccat gttccatttt ggctactgga tggccaagcc atgtaagaag
     5281 atttaactca agtatttatt ctttatgtta ttcagatttc tttcaggctt gtgaactgca
     5341 ccccaatgtt tgagtttaac cacctgatcc ttacatctat ccctccccgg tgaagcacat
     5401 tccattgcta aaagaaaaag aaacacgaaa ttgcttcctg ttgtctgtat aactgttttg
     5461 atagtttgag atatttgtct ataaatgata tttctcagct caaagatcgt gtaaataatt
     5521 atattccttt gctcaatggg tttatttcta atgaggctgc cagttctgag agattctata
     5581 atatcacttt taaataacat aaacagggat tacaactatg taaaaagaaa tgcatatgga
     5641 caaagactgg gaacacagat aattgaaatc agttgtgtta gggtggtgga attatgtgaa
     5701 tttttttttc tttttaaaat tttatttgat attgttataa tattgcttta caataaataa
     5761 acagcagaaa gggaactata gacacataga aaagatgcca gaagcagatg ccttctggcc
     5821 agagcgcaga gcatgcaggg cagagatatt tgctagttac aattattcca taggctttat
     5881 gcttgcctgg gtgctgaggt tggcacacgc tcgggtatgg cacacgcttt cttaggagac
     5941 tattatctat aagttaaagc tagggagatg tcactattag aactccaaac acactcttct
     6001 gctttaaaac aggttgtctg ccctctgctt tggtatggca ttcgggtgtc tgttttgtgg
     6061 ttgctttaga ttggaggggt gaccatttta ttagccccct tgataacatc tgttgcagat
     6121 attgcctttc tggaacgttt taacagactc tcaggttgaa ttttggagga ctagaaggat
     6181 aaaatcccca gctcccacca ttttcttgtc caacaggata ttactgtata tcattcaggt
     6241 aggattcttc ttttaataac caatagggca agtcccacta atttcaatag aagttatgac
     6301 ttgcaattaa aagctgactt tgaaatcatt aaacaaatat gtaggactgt ctctgcctgt
     6361 tggcattcag ttatagttct gttaattttg gcttgggatg gtctccatgt gcttttttct
     6421 gcctatttat aggttgtttg cagtagttgt gatttttaaa gagcaaggga gaccatctaa
     6481 ccaaaggata acttccttct aactcaccaa agaaatttta ggtgagaact ttaataatga
     6541 ggtagtcacc tcagatatgc tgcttagttt cactaaaagc agaccctata cctagagaag
     6601 tcactggctt tttattggtc attctcaata cagaaatact taggggagtc ttaaccctgc
     6661 catccccggt tgaatctctt ggtctttatc taagctactt gcagttaata ttcagttaag
     6721 caaaggtatg gccagtagtg caagtatctc ccagtctctg agctctgaac aagaggactg
     6781 aaattcagca tttgtaaact gacagtttga tgggcctggg atttgaagtg aactcagcac
     6841 acaattctga acgtgtattt gcatgtggac tgggaaggaa ataaatggga acttggaaat
     6901 aatggaatat ttctcctatg aaagaatttt tcgtagaaga tttgtttttg atataatctt
     6961 tctgttggtt agcttttagt gttttcattc cttttctgat ccacactcct ttaagtgacc
     7021 aaatgaatat aacccaacat gcattgggaa tgtgtttaat attaaacaat gtctaactga
     7081 atctgcaaat gcgggaactg agatatcacc tccatgtgca cacctgtgtg tacgagtatt
     7141 ctatacaact tgtagcattt actgccactt aattgggttg aacttgcaag ataaactttt
     7201 ggaaactgct tagtgccatc ggagtctcct ttagaagctg ccatcaggca aatgctatcc
     7261 cataatacca gcagtaagcc tggcaacatg ttcaacagat ttagtaccca agaggaaatc
     7321 aacagcgata gtagagaatg agtcagatgt agtgggataa atactagcct aggaagaagg
     7381 agccccggag tctaatatga gctttattac taaattgcta tgtgacgcta ggcaagtcac
     7441 ttaacctctc catggctgtt tcctcatctg taaaataagt gtattggact agatgatcct
     7501 tagggtcttt ccaaaagtct aacattctat ggcattatag gttgccttgc aaattcagcc
     7561 tgctatagtg atggcaaata tcacgtttaa gcctgagtct cttatgttgc agttaaataa
     7621 aagaactatg taagatgatt tttaaaattc aagcaaatgg gccgggtgcg gtggctcata
     7681 cctgtaatcc cagcactttg ggaggccaag gcaggcggat cacctgaggt caggagttcg
     7741 agaccagcct gaccaacata gagaaacccc atctctacta aaaatacaaa attagccggg
     7801 tgtggtggcg ggcgcctgta atcccagcta cttgggaggc tgaggtggga gaatcgcttg
     7861 aacccaggag gcggaggttg tggtgagctg agatcatgcc attgcactcc agcctcggca
     7921 acaagagtga aacttcgtct ccaaaaaaaa aaactcaagc aaatgaagtt cataataata
     7981 ggggatgttg ataaaacttg tggcagcctt ccaattcatt tacagttgtt tcgttttgtt
     8041 tttgttttaa tgtccatttt ctgttgactg ttcccagttt tcattttcca tacagtctgt
     8101 atgtaaagtc tggttttcat taagctgtgg ccagtatttg ccactacaac agaaacacac
     8161 tgtcacactt gctagaatat aactgtactt gagcttctcc tttcctgtga agtagtgctg
     8221 ggctttctag agtttaattc tcaagtggca caagatagca gagcccatgc attttaatgg
     8281 ctgagactgc taagagtgaa cctaaacact tacaagttgc agagagaaat gaaaaagtaa
     8341 ttacatgcta ttagcattga gaaatgttga caaattaatt tgttgggaac caaagatagc
     8401 atttctgatg acaactccca cagtgattgg ccagttgtat gatgagtaca ctgctggaaa
     8461 gagggtaaac tgggagttag tggatggtcc caatgccctg cctacagcag agtgccaacc
     8521 agccctgagt gcaaaattca agttcaatgt gtgtgcttgt gtgtggtgtg ctttatggac
     8581 ccgcaaatac catattcatt attgatgata agatcttcac agaatcctgt agctactaat
     8641 gcattgagtt tttaatctca gtacatcagc caggaggagc cagatcacag ggtagtgatg
     8701 tctactggga ttatactcat aacatctaca caaaacaagt tgagaaggat ccacgttttc
     8761 attgtttatc agaattgtat ctcatttggc tgagcattac ttttgtcaga atgtgttatc
     8821 tgtaaaccat gtgtagtgaa attcttctgt aactttggat taaaggtatt tatggtcttt
     8881 ttgtttgttt gatttttaag taagttattt cttttgtaga cctgctgatg gtatggttcc
     8941 atccttctga cctcagcatc caatcttttt aaggattttt gttttcaata ttgttatttt
     9001 aaattgtggt tgaagcaata gaaaattgaa atatggattg tgcatgactg tgtcttgagt
     9061 gtaaaaatat tgcagtttga aacttggacc taaagtattg caaataaaaa tgacaaacat
     9121 caatga
//
