LOCUS       NM_001376279            3178 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens p21 (RAC1) activated kinase 1 (PAK1), transcript
            variant 14, mRNA.
ACCESSION   NM_001376279 XM_017017850
VERSION     NM_001376279.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Kervarrec,T., Westphal,D., Pissaloux,D., Legrand,M., Tirode,F.,
            Neuhart,A., Drouot,F., Becker,J.C., Macagno,N., Seris,A.,
            Jouary,T., Beltzung,F., Jullie,M.L., Harms,P.W., Cribier,B.,
            Mourah,S., Jouenne,F., Fromont,G., Louveau,B., Mancini,M.,
            Kazakov,D.V., de la Fouchardiere,A. and Battistella,M.
  TITLE     Porocarcinomas with PAK1/2/3 fusions: a series of 12 cases
  JOURNAL   Histopathology 85 (4), 566-578 (2024)
   PUBMED   38785043
  REMARK    GeneRIF: Porocarcinomas with PAK1/2/3 fusions: a series of 12
            cases.
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Zhao,P., Zhang,J., Song,W., Qi,D., Huang,Y., Su,Y., Wu,R., Zhang,L.
            and Zhang,S.
  TITLE     Incarvine C and its analogues inhibit the formation of cell
            cytoskeleton by targeting Rac1
  JOURNAL   Bioorg Chem 149, 107512 (2024)
   PUBMED   38833990
REFERENCE   3  (bases 1 to 3178)
  AUTHORS   Xu,M.H., Zheng,Y.M., Liang,B.G., Xu,W.X., Cao,J., Wang,P.,
            Dong,Z.Y., Zhou,C.H., Sun,H.C., Ren,N., Ke,A.W. and Shen,Y.H.
  TITLE     Deubiquitination of CIB1 by USP14 promotes lenvatinib resistance
            via the PAK1-ERK1/2 axis in hepatocellular carcinoma
  JOURNAL   Int J Biol Sci 20 (9), 3269-3284 (2024)
   PUBMED   38993552
  REMARK    GeneRIF: Deubiquitination of CIB1 by USP14 promotes lenvatinib
            resistance via the PAK1-ERK1/2 axis in hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3178)
  AUTHORS   Luo,Q., Raulston,E.G., Prado,M.A., Wu,X., Gritsman,K., Whalen,K.S.,
            Yan,K., Booth,C.A.G., Xu,R., van Galen,P., Doench,J.G., Shimony,S.,
            Long,H.W., Neuberg,D.S., Paulo,J.A. and Lane,A.A.
  TITLE     Targetable leukaemia dependency on noncanonical PI3Kgamma
            signalling
  JOURNAL   Nature 630 (8015), 198-205 (2024)
   PUBMED   38720074
  REMARK    GeneRIF: Targetable leukaemia dependency on noncanonical PI3Kgamma
            signalling.
REFERENCE   5  (bases 1 to 3178)
  AUTHORS   Chen,M., Pan,L., Chen,D., Wu,Y., Ye,J., Li,K., Zhang,N. and Xu,J.
  TITLE     PAK1 Promotes Inflammation Induced by Sepsis through the
            Snail/CXCL2 Signaling Pathway
  JOURNAL   ACS Infect Dis 10 (4), 1370-1378 (2024)
   PUBMED   38503263
  REMARK    GeneRIF: PAK1 Promotes Inflammation Induced by Sepsis through the
            Snail/CXCL2 Signaling Pathway.
REFERENCE   6  (bases 1 to 3178)
  AUTHORS   Manser,E., Huang,H.Y., Loo,T.H., Chen,X.Q., Dong,J.M., Leung,T. and
            Lim,L.
  TITLE     Expression of constitutively active alpha-PAK reveals effects of
            the kinase on actin and focal complexes
  JOURNAL   Mol Cell Biol 17 (3), 1129-1143 (1997)
   PUBMED   9032240
REFERENCE   7  (bases 1 to 3178)
  AUTHORS   Bokoch,G.M., Wang,Y., Bohl,B.P., Sells,M.A., Quilliam,L.A. and
            Knaus,U.G.
  TITLE     Interaction of the Nck adapter protein with p21-activated kinase
            (PAK1)
  JOURNAL   J Biol Chem 271 (42), 25746-25749 (1996)
   PUBMED   8824201
REFERENCE   8  (bases 1 to 3178)
  AUTHORS   Brown,J.L., Stowers,L., Baer,M., Trejo,J., Coughlin,S. and Chant,J.
  TITLE     Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase
            pathway
  JOURNAL   Curr Biol 6 (5), 598-605 (1996)
   PUBMED   8805275
REFERENCE   9  (bases 1 to 3178)
  AUTHORS   Martin,G.A., Bollag,G., McCormick,F. and Abo,A.
  TITLE     A novel serine kinase activated by rac1/CDC42Hs-dependent
            autophosphorylation is related to PAK65 and STE20
  JOURNAL   EMBO J 14 (9), 1970-1978 (1995)
   PUBMED   7744004
  REMARK    Erratum:[EMBO J. 1995 Sep 1;14(17):4385. doi:
            10.1002/j.1460-2075.1995.tb00113.x.. PMID: 7556080]
REFERENCE   10 (bases 1 to 3178)
  AUTHORS   Manser,E., Leung,T., Salihuddin,H., Zhao,Z.S. and Lim,L.
  TITLE     A brain serine/threonine protein kinase activated by Cdc42 and Rac1
  JOURNAL   Nature 367 (6458), 40-46 (1994)
   PUBMED   8107774
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP000486.6.
            
            On Nov 14, 2019 this sequence version replaced XM_017017850.1.
            
            Summary: This gene encodes a family member of serine/threonine
            p21-activating kinases, known as PAK proteins. These proteins are
            critical effectors that link RhoGTPases to cytoskeleton
            reorganization and nuclear signaling, and they serve as targets for
            the small GTP binding proteins Cdc42 and Rac. This specific family
            member regulates cell motility and morphology. Mutations in this
            gene have been associated with macrocephaly, seizures, and speech
            delay. Overexpression of this gene is also reported in many cancer
            types, and particularly in breast cancer. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Aug
            2020].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.123052.1,
                                           SRR14038194.3607112.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-198               AP000486.6         92036-92233         c
            199-265             AP000486.6         77289-77355         c
            266-473             AP000486.6         72593-72800         c
            474-574             AP000486.6         60156-60256         c
            575-722             AP000486.6         59503-59650         c
            723-760             AP000486.6         54590-54627         c
            761-880             AP000486.6         39160-39279         c
            881-1055            AP000486.6         35930-36104         c
            1056-1119           AP000486.6         33798-33861         c
            1120-1168           AP000486.6         29501-29549         c
            1169-1281           AP000486.6         24081-24193         c
            1282-1399           AP000486.6         20908-21025         c
            1400-1499           AP000486.6         17586-17685         c
            1500-1696           AP000486.6         16348-16544         c
            1697-1834           AP000486.6         12992-13129         c
            1835-3178           AP000486.6         2279-3622           c
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.5-q14.1"
     gene            1..3178
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /note="p21 (RAC1) activated kinase 1"
                     /db_xref="GeneID:5058"
                     /db_xref="HGNC:HGNC:8590"
                     /db_xref="MIM:602590"
     exon            1..198
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            199..265
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            266..473
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             284..1921
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 14;
                     p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related);
                     p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast);
                     serine/threonine-protein kinase PAK 1; p21 protein
                     (Cdc42/Rac)-activated kinase 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase PAK 1 isoform 2"
                     /protein_id="NP_001363208.1"
                     /db_xref="CCDS:CCDS8250.1"
                     /db_xref="GeneID:5058"
                     /db_xref="HGNC:HGNC:8590"
                     /db_xref="MIM:602590"
                     /translation="MSNNGLDIQDKPPAPPMRNTSTMIGAGSKDAGTLNHGSKPLPPN
                     PEEKKKKDRFYRSILPGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQ
                     WARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQKYMSFTDKSAEDYNSSNALN
                     VKAVSETPAVPPVSEDEDDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPVTPTRDVA
                     TSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKI
                     GQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSY
                     LVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKS
                     DNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSL
                     GIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEK
                     RGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH"
     exon            474..574
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            575..722
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            723..760
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            761..880
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1055
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1119
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1168
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1281
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1399
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1499
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1696
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1834
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..3178
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2192..2197
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /note="hexamer: AATAAA"
     polyA_site      2223
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /note="major polyA site"
     regulatory      3156..3161
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /note="hexamer: AATAAA"
     polyA_site      3178
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /note="major polyA site"
ORIGIN      
        1 gccttcgctg ccgccctgcg agagcgcgag cgtgctgcac ctcgggagct ggcggctgag
       61 ctgccaggag cccagcccag ctgaatcgag cggagccggt ggagcagggg ccttgtgggt
      121 acccggttgg gcagggagag gtgcggctct gcgacggaaa caatcgccag agatgccggg
      181 gctagccttc cccaccagca cgtctgaggc attggttttt catccttcca aacttttggt
      241 ctacagagac tattagaaga ttaagctgct gctggtggtg acaatgtcaa ataacggcct
      301 agacattcaa gacaaacccc cagcccctcc gatgagaaat accagcacta tgattggagc
      361 cggcagcaaa gatgctggaa ccctaaacca tggttctaaa cctctgcctc caaacccaga
      421 ggagaagaaa aagaaggacc gattttaccg atccatttta cctggagata aaacaaataa
      481 aaagaaagag aaagagcggc cagagatttc tctcccttca gattttgaac acacaattca
      541 tgtcggtttt gatgctgtca caggggagtt tacgggaatg ccagagcagt gggcccgctt
      601 gcttcagaca tcaaatatca ctaagtcgga gcagaagaaa aacccgcagg ctgttctgga
      661 tgtgttggag ttttacaact cgaagaagac atccaacagc cagaaataca tgagctttac
      721 agataagtca gctgaggatt acaattcttc taatgccttg aatgtgaagg ctgtgtctga
      781 gactcctgca gtgccaccag tttcagaaga tgaggatgat gatgatgatg atgctacccc
      841 accaccagtg attgctccac gcccagagca cacaaaatct gtatacacac ggtctgtgat
      901 tgaaccactt cctgtcactc caactcggga cgtggctaca tctcccattt cacctactga
      961 aaataacacc actccaccag atgctttgac ccggaatact gagaagcaga agaagaagcc
     1021 taaaatgtct gatgaggaga tcttggagaa attacgaagc atagtgagtg tgggcgatcc
     1081 taagaagaaa tatacacggt ttgagaagat tggacaaggt gcttcaggca ccgtgtacac
     1141 agcaatggat gtggccacag gacaggaggt ggccattaag cagatgaatc ttcagcagca
     1201 gcccaagaaa gagctgatta ttaatgagat cctggtcatg agggaaaaca agaacccaaa
     1261 cattgtgaat tacttggaca gttacctcgt gggagatgag ctgtgggttg ttatggaata
     1321 cttggctgga ggctccttga cagatgtggt gacagaaact tgcatggatg aaggccaaat
     1381 tgcagctgtg tgccgtgagt gtctgcaggc tctggagttc ttgcattcga accaggtcat
     1441 tcacagagac atcaagagtg acaatattct gttgggaatg gatggctctg tcaagctaac
     1501 tgactttgga ttctgtgcac agataacccc agagcagagc aaacggagca ccatggtagg
     1561 aaccccatac tggatggcac cagaggttgt gacacgaaag gcctatgggc ccaaggttga
     1621 catctggtcc ctgggcatca tggccatcga aatgattgaa ggggagcctc catacctcaa
     1681 tgaaaaccct ctgagagcct tgtacctcat tgccaccaat gggaccccag aacttcagaa
     1741 cccagagaag ctgtcagcta tcttccggga ctttctgaac cgctgtctcg agatggatgt
     1801 ggagaagaga ggttcagcta aagagctgct acagcatcaa ttcctgaaga ttgccaagcc
     1861 cctctccagc ctcactccac tgattgctgc agctaaggag gcaacaaaga acaatcacta
     1921 aaaccacact caccccagcc tcattgtgcc aagccttctg tgagataaat gcacatttca
     1981 gaaattccaa ctcctgatgc cctcttctcc ttgccttgct tctcccattt cctgatctag
     2041 cactcctcaa gactttgatc cttggaaacc gtgtgtccag cattgaagag aactgcaact
     2101 gaatgactaa tcagatgatg gccatttcta aataaggaat ttcctcccaa ttcatggata
     2161 tgagggtggt ttatgattaa gggtttatat aaataaatgt ttctagtctt ccgtgtgtca
     2221 aaatcctcac ctccttcata accatctccc acaattaatt cttgactata taaatttatg
     2281 gtttgataat attatcaatt tgtaatcaat tgagatttct ttagtgcttg cttttctgtg
     2341 actcaactgc ccagacacct cattgtactt gaaaactgga acagcttggg aatgccatgg
     2401 ggtttgataa tctgccaggg acatgaagag gctcagcttc ctggaccatg actttggctc
     2461 agctgatcct gacatgggag aacaaccaca tttttctttg tgtgtgcttc tagcagctgt
     2521 tcgggaggac cttgacccaa tagtgttccc atgctgtttc ttgtgaaatg ctctcggcta
     2581 tgtagcagct tttgattccc tgcataccct aggctgctgc ccctatcctg tcccttgttt
     2641 ataacattga gaggttttct agggcacata ctgagtgaga gcagtgttga gaagtcgggg
     2701 aaaatggtga ctacttttag agcaaggctg ggcatcagca cctgtccagc tctacttgtg
     2761 tgatgtttca ggaactcagc ccctttttct gcctaggata aggagctgaa agattaactt
     2821 ggatcttcta atggtccaaa tcttttggtc acaataaaga gtctccaaat tagagactgc
     2881 atgttagttc tggatggatt tggtggcctg acatgatacc ctgccagctg tgaggggacc
     2941 ccgtttttaa gatgcatggc caagctctct gcaaatggaa atgcttacac tgggtgttgg
     3001 ggatgtttgc tacctcctgc tatttttgtg gttttggttc tcccactatg gtaggacccc
     3061 tggccagcat tgtggcttgt catgtcagcc ccattgacta ccttctcatg ctctgaggta
     3121 ctactgcctc tgcagcacaa atttctattt ctgtcaataa aaggagatga aaatattc
//
