LOCUS       NM_001376277            3602 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens p21 (RAC1) activated kinase 1 (PAK1), transcript
            variant 12, mRNA.
ACCESSION   NM_001376277 XM_024448564
VERSION     NM_001376277.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Kervarrec,T., Westphal,D., Pissaloux,D., Legrand,M., Tirode,F.,
            Neuhart,A., Drouot,F., Becker,J.C., Macagno,N., Seris,A.,
            Jouary,T., Beltzung,F., Jullie,M.L., Harms,P.W., Cribier,B.,
            Mourah,S., Jouenne,F., Fromont,G., Louveau,B., Mancini,M.,
            Kazakov,D.V., de la Fouchardiere,A. and Battistella,M.
  TITLE     Porocarcinomas with PAK1/2/3 fusions: a series of 12 cases
  JOURNAL   Histopathology 85 (4), 566-578 (2024)
   PUBMED   38785043
  REMARK    GeneRIF: Porocarcinomas with PAK1/2/3 fusions: a series of 12
            cases.
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Zhao,P., Zhang,J., Song,W., Qi,D., Huang,Y., Su,Y., Wu,R., Zhang,L.
            and Zhang,S.
  TITLE     Incarvine C and its analogues inhibit the formation of cell
            cytoskeleton by targeting Rac1
  JOURNAL   Bioorg Chem 149, 107512 (2024)
   PUBMED   38833990
REFERENCE   3  (bases 1 to 3602)
  AUTHORS   Xu,M.H., Zheng,Y.M., Liang,B.G., Xu,W.X., Cao,J., Wang,P.,
            Dong,Z.Y., Zhou,C.H., Sun,H.C., Ren,N., Ke,A.W. and Shen,Y.H.
  TITLE     Deubiquitination of CIB1 by USP14 promotes lenvatinib resistance
            via the PAK1-ERK1/2 axis in hepatocellular carcinoma
  JOURNAL   Int J Biol Sci 20 (9), 3269-3284 (2024)
   PUBMED   38993552
  REMARK    GeneRIF: Deubiquitination of CIB1 by USP14 promotes lenvatinib
            resistance via the PAK1-ERK1/2 axis in hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3602)
  AUTHORS   Luo,Q., Raulston,E.G., Prado,M.A., Wu,X., Gritsman,K., Whalen,K.S.,
            Yan,K., Booth,C.A.G., Xu,R., van Galen,P., Doench,J.G., Shimony,S.,
            Long,H.W., Neuberg,D.S., Paulo,J.A. and Lane,A.A.
  TITLE     Targetable leukaemia dependency on noncanonical PI3Kgamma
            signalling
  JOURNAL   Nature 630 (8015), 198-205 (2024)
   PUBMED   38720074
  REMARK    GeneRIF: Targetable leukaemia dependency on noncanonical PI3Kgamma
            signalling.
REFERENCE   5  (bases 1 to 3602)
  AUTHORS   Chen,M., Pan,L., Chen,D., Wu,Y., Ye,J., Li,K., Zhang,N. and Xu,J.
  TITLE     PAK1 Promotes Inflammation Induced by Sepsis through the
            Snail/CXCL2 Signaling Pathway
  JOURNAL   ACS Infect Dis 10 (4), 1370-1378 (2024)
   PUBMED   38503263
  REMARK    GeneRIF: PAK1 Promotes Inflammation Induced by Sepsis through the
            Snail/CXCL2 Signaling Pathway.
REFERENCE   6  (bases 1 to 3602)
  AUTHORS   Manser,E., Huang,H.Y., Loo,T.H., Chen,X.Q., Dong,J.M., Leung,T. and
            Lim,L.
  TITLE     Expression of constitutively active alpha-PAK reveals effects of
            the kinase on actin and focal complexes
  JOURNAL   Mol Cell Biol 17 (3), 1129-1143 (1997)
   PUBMED   9032240
REFERENCE   7  (bases 1 to 3602)
  AUTHORS   Bokoch,G.M., Wang,Y., Bohl,B.P., Sells,M.A., Quilliam,L.A. and
            Knaus,U.G.
  TITLE     Interaction of the Nck adapter protein with p21-activated kinase
            (PAK1)
  JOURNAL   J Biol Chem 271 (42), 25746-25749 (1996)
   PUBMED   8824201
REFERENCE   8  (bases 1 to 3602)
  AUTHORS   Brown,J.L., Stowers,L., Baer,M., Trejo,J., Coughlin,S. and Chant,J.
  TITLE     Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase
            pathway
  JOURNAL   Curr Biol 6 (5), 598-605 (1996)
   PUBMED   8805275
REFERENCE   9  (bases 1 to 3602)
  AUTHORS   Martin,G.A., Bollag,G., McCormick,F. and Abo,A.
  TITLE     A novel serine kinase activated by rac1/CDC42Hs-dependent
            autophosphorylation is related to PAK65 and STE20
  JOURNAL   EMBO J 14 (9), 1970-1978 (1995)
   PUBMED   7744004
  REMARK    Erratum:[EMBO J. 1995 Sep 1;14(17):4385. doi:
            10.1002/j.1460-2075.1995.tb00113.x.. PMID: 7556080]
REFERENCE   10 (bases 1 to 3602)
  AUTHORS   Manser,E., Leung,T., Salihuddin,H., Zhao,Z.S. and Lim,L.
  TITLE     A brain serine/threonine protein kinase activated by Cdc42 and Rac1
  JOURNAL   Nature 367 (6458), 40-46 (1994)
   PUBMED   8107774
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP003680.2 and AP000486.6.
            
            On Nov 13, 2019 this sequence version replaced XM_024448564.1.
            
            Summary: This gene encodes a family member of serine/threonine
            p21-activating kinases, known as PAK proteins. These proteins are
            critical effectors that link RhoGTPases to cytoskeleton
            reorganization and nuclear signaling, and they serve as targets for
            the small GTP binding proteins Cdc42 and Rac. This specific family
            member regulates cell motility and morphology. Mutations in this
            gene have been associated with macrocephaly, seizures, and speech
            delay. Overexpression of this gene is also reported in many cancer
            types, and particularly in breast cancer. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Aug
            2020].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.3974583.1,
                                           SRR18074969.3228482.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-686               AP003680.2         3452-4137           c
            687-897             AP000486.6         72593-72803         c
            898-998             AP000486.6         60156-60256         c
            999-1146            AP000486.6         59503-59650         c
            1147-1184           AP000486.6         54590-54627         c
            1185-1304           AP000486.6         39160-39279         c
            1305-1479           AP000486.6         35930-36104         c
            1480-1543           AP000486.6         33798-33861         c
            1544-1592           AP000486.6         29501-29549         c
            1593-1705           AP000486.6         24081-24193         c
            1706-1823           AP000486.6         20908-21025         c
            1824-1923           AP000486.6         17586-17685         c
            1924-2120           AP000486.6         16348-16544         c
            2121-2258           AP000486.6         12992-13129         c
            2259-3602           AP000486.6         2279-3622           c
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.5-q14.1"
     gene            1..3602
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /note="p21 (RAC1) activated kinase 1"
                     /db_xref="GeneID:5058"
                     /db_xref="HGNC:HGNC:8590"
                     /db_xref="MIM:602590"
     exon            1..686
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            687..897
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             708..2345
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 12;
                     p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related);
                     p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast);
                     serine/threonine-protein kinase PAK 1; p21 protein
                     (Cdc42/Rac)-activated kinase 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase PAK 1 isoform 2"
                     /protein_id="NP_001363206.1"
                     /db_xref="CCDS:CCDS8250.1"
                     /db_xref="GeneID:5058"
                     /db_xref="HGNC:HGNC:8590"
                     /db_xref="MIM:602590"
                     /translation="MSNNGLDIQDKPPAPPMRNTSTMIGAGSKDAGTLNHGSKPLPPN
                     PEEKKKKDRFYRSILPGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQ
                     WARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQKYMSFTDKSAEDYNSSNALN
                     VKAVSETPAVPPVSEDEDDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPVTPTRDVA
                     TSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKI
                     GQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSY
                     LVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKS
                     DNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSL
                     GIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEK
                     RGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH"
     exon            898..998
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1146
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1184
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1304
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1479
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1543
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1592
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1705
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1823
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1923
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1924..2120
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2121..2258
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..3602
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2616..2621
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /note="hexamer: AATAAA"
     polyA_site      2647
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /note="major polyA site"
     regulatory      3580..3585
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /note="hexamer: AATAAA"
     polyA_site      3602
                     /gene="PAK1"
                     /gene_synonym="alpha-PAK; IDDMSSD; p65-PAK; PAKalpha"
                     /note="major polyA site"
ORIGIN      
        1 gtacaatagc gcggctgtgg gcgggggagg ctgccctccc gcggctgcag ccggagccga
       61 aggtggtggc tgcacagtag acgccccctc acggcttccc ccacacgctc ccgccccctc
      121 gctcgcccat cgcgcttccc tcacaggctc tgcagtcctc ccccacagac gccttccccc
      181 ttggactctc attccctttt ccacggagcc ccgcgctttc gtgagccccc tcgaggaacc
      241 tggtctccgc atccagttac cacctcctgc ctcagaggcc atctgagccc ttcgcacctc
      301 gcccctcagt ccccccttcc ccccccgccc gcgtcgcctc gctccctccc gcccccccat
      361 catcccttcc ctcgcagttc ccctgtcctg aggggagccc cgccacgggc agcgcggcgg
      421 cggcggcagg agggagaaag tgaagcggta gctcgcgcac actcgcgccc tcactcccgg
      481 ctaggcggca cccaccgccg ggaggaggag gaggagccga gaggagctga gcgagcgcgg
      541 aaggtgagag cgggcctacc gcccagtacc cctcgcgtcc cgggctgcca gggctggggc
      601 gcgggggtgg ggacgggtgg agtgggggtc tgggcccatt tatttcgcgt tgcggggccg
      661 ggcgcctggg cgggaggagg cgccagtagc tgctgctggt ggtgacaatg tcaaataacg
      721 gcctagacat tcaagacaaa cccccagccc ctccgatgag aaataccagc actatgattg
      781 gagccggcag caaagatgct ggaaccctaa accatggttc taaacctctg cctccaaacc
      841 cagaggagaa gaaaaagaag gaccgatttt accgatccat tttacctgga gataaaacaa
      901 ataaaaagaa agagaaagag cggccagaga tttctctccc ttcagatttt gaacacacaa
      961 ttcatgtcgg ttttgatgct gtcacagggg agtttacggg aatgccagag cagtgggccc
     1021 gcttgcttca gacatcaaat atcactaagt cggagcagaa gaaaaacccg caggctgttc
     1081 tggatgtgtt ggagttttac aactcgaaga agacatccaa cagccagaaa tacatgagct
     1141 ttacagataa gtcagctgag gattacaatt cttctaatgc cttgaatgtg aaggctgtgt
     1201 ctgagactcc tgcagtgcca ccagtttcag aagatgagga tgatgatgat gatgatgcta
     1261 ccccaccacc agtgattgct ccacgcccag agcacacaaa atctgtatac acacggtctg
     1321 tgattgaacc acttcctgtc actccaactc gggacgtggc tacatctccc atttcaccta
     1381 ctgaaaataa caccactcca ccagatgctt tgacccggaa tactgagaag cagaagaaga
     1441 agcctaaaat gtctgatgag gagatcttgg agaaattacg aagcatagtg agtgtgggcg
     1501 atcctaagaa gaaatataca cggtttgaga agattggaca aggtgcttca ggcaccgtgt
     1561 acacagcaat ggatgtggcc acaggacagg aggtggccat taagcagatg aatcttcagc
     1621 agcagcccaa gaaagagctg attattaatg agatcctggt catgagggaa aacaagaacc
     1681 caaacattgt gaattacttg gacagttacc tcgtgggaga tgagctgtgg gttgttatgg
     1741 aatacttggc tggaggctcc ttgacagatg tggtgacaga aacttgcatg gatgaaggcc
     1801 aaattgcagc tgtgtgccgt gagtgtctgc aggctctgga gttcttgcat tcgaaccagg
     1861 tcattcacag agacatcaag agtgacaata ttctgttggg aatggatggc tctgtcaagc
     1921 taactgactt tggattctgt gcacagataa ccccagagca gagcaaacgg agcaccatgg
     1981 taggaacccc atactggatg gcaccagagg ttgtgacacg aaaggcctat gggcccaagg
     2041 ttgacatctg gtccctgggc atcatggcca tcgaaatgat tgaaggggag cctccatacc
     2101 tcaatgaaaa ccctctgaga gccttgtacc tcattgccac caatgggacc ccagaacttc
     2161 agaacccaga gaagctgtca gctatcttcc gggactttct gaaccgctgt ctcgagatgg
     2221 atgtggagaa gagaggttca gctaaagagc tgctacagca tcaattcctg aagattgcca
     2281 agcccctctc cagcctcact ccactgattg ctgcagctaa ggaggcaaca aagaacaatc
     2341 actaaaacca cactcacccc agcctcattg tgccaagcct tctgtgagat aaatgcacat
     2401 ttcagaaatt ccaactcctg atgccctctt ctccttgcct tgcttctccc atttcctgat
     2461 ctagcactcc tcaagacttt gatccttgga aaccgtgtgt ccagcattga agagaactgc
     2521 aactgaatga ctaatcagat gatggccatt tctaaataag gaatttcctc ccaattcatg
     2581 gatatgaggg tggtttatga ttaagggttt atataaataa atgtttctag tcttccgtgt
     2641 gtcaaaatcc tcacctcctt cataaccatc tcccacaatt aattcttgac tatataaatt
     2701 tatggtttga taatattatc aatttgtaat caattgagat ttctttagtg cttgcttttc
     2761 tgtgactcaa ctgcccagac acctcattgt acttgaaaac tggaacagct tgggaatgcc
     2821 atggggtttg ataatctgcc agggacatga agaggctcag cttcctggac catgactttg
     2881 gctcagctga tcctgacatg ggagaacaac cacatttttc tttgtgtgtg cttctagcag
     2941 ctgttcggga ggaccttgac ccaatagtgt tcccatgctg tttcttgtga aatgctctcg
     3001 gctatgtagc agcttttgat tccctgcata ccctaggctg ctgcccctat cctgtccctt
     3061 gtttataaca ttgagaggtt ttctagggca catactgagt gagagcagtg ttgagaagtc
     3121 ggggaaaatg gtgactactt ttagagcaag gctgggcatc agcacctgtc cagctctact
     3181 tgtgtgatgt ttcaggaact cagccccttt ttctgcctag gataaggagc tgaaagatta
     3241 acttggatct tctaatggtc caaatctttt ggtcacaata aagagtctcc aaattagaga
     3301 ctgcatgtta gttctggatg gatttggtgg cctgacatga taccctgcca gctgtgaggg
     3361 gaccccgttt ttaagatgca tggccaagct ctctgcaaat ggaaatgctt acactgggtg
     3421 ttggggatgt ttgctacctc ctgctatttt tgtggttttg gttctcccac tatggtagga
     3481 cccctggcca gcattgtggc ttgtcatgtc agccccattg actaccttct catgctctga
     3541 ggtactactg cctctgcagc acaaatttct atttctgtca ataaaaggag atgaaaatat
     3601 tc
//
