LOCUS       NM_000437               3487 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens platelet activating factor acetylhydrolase 2 (PAFAH2),
            mRNA.
ACCESSION   NM_000437
VERSION     NM_000437.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Moschos,M.M., Pantazis,P., Gatzioufas,Z., Panos,G.D., Gazouli,M.,
            Nitoda,E. and Brouzas,D.
  TITLE     'Association between platelet activating factor acetylhydrolase and
            diabetic retinopathy: Does inflammation affect the retinal status?'
  JOURNAL   Prostaglandins Other Lipid Mediat 122, 69-72 (2016)
   PUBMED   26791393
  REMARK    GeneRIF: Data show that the activity of plasma platelet activating
            factor acetylhydrolase (PAF-AH) increases in parallel with diabetic
            retinopathy (DR) severity.
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Monillas,E.S., Caplan,J.L., Thevenin,A.F. and Bahnson,B.J.
  TITLE     Oligomeric state regulated trafficking of human platelet-activating
            factor acetylhydrolase type-II
  JOURNAL   Biochim Biophys Acta 1854 (5), 469-475 (2015)
   PUBMED   25707358
  REMARK    GeneRIF: The oligomeric state of PAFAH-II drives functional protein
            trafficking. PAFAH-II localization to the membrane is critical for
            substrate acquisition and effective oxidative stress protection.
REFERENCE   3  (bases 1 to 3487)
  AUTHORS   Schmidt,E.B., Koenig,W., Khuseyinova,N. and Christensen,J.H.
  TITLE     Lipoprotein-associated phospholipase A2 concentrations in plasma
            are associated with the extent of coronary artery disease and
            correlate to adipose tissue levels of marine n-3 fatty acids
  JOURNAL   Atherosclerosis 196 (1), 420-424 (2008)
   PUBMED   17157859
  REMARK    GeneRIF: Lp-PLA2 was higher in those with more extensive Coronary
            artery disease
REFERENCE   4  (bases 1 to 3487)
  AUTHORS   Umemura,K., Kato,I., Hirashima,Y., Ishii,Y., Inoue,T., Aoki,J.,
            Kono,N., Oya,T., Hayashi,N., Hamada,H., Endo,S., Oda,M., Arai,H.,
            Kinouchi,H. and Hiraga,K.
  TITLE     Neuroprotective role of transgenic PAF-acetylhydrolase II in mouse
            models of focal cerebral ischemia
  JOURNAL   Stroke 38 (3), 1063-1068 (2007)
   PUBMED   17272759
  REMARK    GeneRIF: PAF-AH II exerts strong neuroprotective effects against
            ischemic injury: the neurological deficit scores, cerebral edema
            index, and relative infarction volume are all significantly lower
            in PAFAH2 transgenic mice than in wild-type mice.
REFERENCE   5  (bases 1 to 3487)
  AUTHORS   Unno,N., Sakaguchi,T., Nakamura,T., Yamamoto,N., Sugatani,J.,
            Miwa,M. and Konno,H.
  TITLE     A single nucleotide polymorphism in the plasma PAF acetylhydrolase
            gene and risk of atherosclerosis in Japanese patients with
            peripheral artery occlusive disease
  JOURNAL   J Surg Res 134 (1), 36-43 (2006)
   PUBMED   16650870
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 3487)
  AUTHORS   Marques,M., Pei,Y., Southall,M.D., Johnston,J.M., Arai,H., Aoki,J.,
            Inoue,T., Seltmann,H., Zouboulis,C.C. and Travers,J.B.
  TITLE     Identification of platelet-activating factor acetylhydrolase II in
            human skin
  JOURNAL   J Invest Dermatol 119 (4), 913-919 (2002)
   PUBMED   12406338
  REMARK    GeneRIF: presence of platelet-activating factor acetylhydrolase II
            in a restricted pattern in human skin; it is involved in protecting
            this organ against oxidative stress through the degradation of
            oxidatively modified bioactive phospholipids.
REFERENCE   7  (bases 1 to 3487)
  AUTHORS   Min,J.H., Wilder,C., Aoki,J., Arai,H., Inoue,K., Paul,L. and
            Gelb,M.H.
  TITLE     Platelet-activating factor acetylhydrolases: broad substrate
            specificity and lipoprotein binding does not modulate the catalytic
            properties of the plasma enzyme
  JOURNAL   Biochemistry 40 (15), 4539-4549 (2001)
   PUBMED   11294621
  REMARK    GeneRIF: examines substrate specificity for intracellular and
            plasma PAF-AHs
REFERENCE   8  (bases 1 to 3487)
  AUTHORS   Rice,S.Q., Southan,C., Boyd,H.F., Terrett,J.A., MacPhee,C.H.,
            Moores,K., Gloger,I.S. and Tew,D.G.
  TITLE     Expression, purification and characterization of a human
            serine-dependent phospholipase A2 with high specificity for
            oxidized phospholipids and platelet activating factor
  JOURNAL   Biochem J 330 (Pt 3) (Pt 3), 1309-1315 (1998)
   PUBMED   9494101
REFERENCE   9  (bases 1 to 3487)
  AUTHORS   Stafforini,D.M., McIntyre,T.M., Zimmerman,G.A. and Prescott,S.M.
  TITLE     Platelet-activating factor acetylhydrolases
  JOURNAL   J Biol Chem 272 (29), 17895-17898 (1997)
   PUBMED   9218411
  REMARK    Review article
REFERENCE   10 (bases 1 to 3487)
  AUTHORS   Hattori,K., Adachi,H., Matsuzawa,A., Yamamoto,K., Tsujimoto,M.,
            Aoki,J., Hattori,M., Arai,H. and Inoue,K.
  TITLE     cDNA cloning and expression of intracellular platelet-activating
            factor (PAF) acetylhydrolase II. Its homology with plasma PAF
            acetylhydrolase
  JOURNAL   J Biol Chem 271 (51), 33032-33038 (1996)
   PUBMED   8955149
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from D87845.1, U89386.1, BC001158.1,
            BU630496.1 and AL592064.14.
            
            On Nov 23, 2018 this sequence version replaced NM_000437.3.
            
            Summary: This gene encodes platelet-activating factor
            acetylhydrolase isoform 2, a single-subunit intracellular enzyme
            that catalyzes the removal of the acetyl group at the SN-2 position
            of platelet-activating factor (identified as
            1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this
            lipase exhibits a broader substrate specificity than simply
            platelet activating factor. Two other isoforms of intracellular
            platelet-activating factor acetylhydrolase exist, and both are
            multi-subunit enzymes. Additionally, there is a single-subunit
            serum isoform of this enzyme. [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.67248.1,
                                           SRR1163657.101110.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374282.8/ ENSP00000363400.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                D87845.1           118-131
            15-39               U89386.1           8-32
            40-2181             BC001158.1         79-2220
            2182-2444           BU630496.1         5-267               c
            2445-3487           AL592064.14        36478-37520         c
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.11"
     gene            1..3487
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /note="platelet activating factor acetylhydrolase 2"
                     /db_xref="GeneID:5051"
                     /db_xref="HGNC:HGNC:8579"
                     /db_xref="MIM:602344"
     exon            1..39
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            40..176
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1265
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /EC_number="3.1.1.47"
                     /EC_number="2.3.1.149"
                     /note="SD-PLA2; serine-dependent phospholipase A2;
                     platelet-activating factor acetylhydrolase 2, 40kDa;
                     PAF:sphingosine transacetylase; PAF:lysophospholipid
                     transacetylase; platelet-activating factor
                     acetyltransferase PAFAH2"
                     /codon_start=1
                     /product="platelet-activating factor acetylhydrolase 2,
                     cytoplasmic"
                     /protein_id="NP_000428.2"
                     /db_xref="CCDS:CCDS270.1"
                     /db_xref="GeneID:5051"
                     /db_xref="HGNC:HGNC:8579"
                     /db_xref="MIM:602344"
                     /translation="MGVNQSVGFPPVTGPHLVGCGDVMEGQNLQGSFFRLFYPCQKAE
                     ETMEQPLWIPRYEYCTGLAEYLQFNKRCGGLLFNLAVGSCRLPVSWNGPFKTKDSGYP
                     LIIFSHGLGAFRTLYSAFCMELASRGFVVAVPEHRDRSAATTYFCKQAPEENQPTNES
                     LQEEWIPFRRVEEGEKEFHVRNPQVHQRVSECLRVLKILQEVTAGQTVFNILPGGLDL
                     MTLKGNIDMSRVAVMGHSFGGATAILALAKETQFRCAVALDAWMFPLERDFYPKARGP
                     VFFINTEKFQTMESVNLMKKICAQHEQSRIITVLGSVHRSQTDFAFVTGNLIGKFFST
                     ETRGSLDPYEGQEVMVRAMLAFLQKHLDLKEDYNQWNNLIEGIGPSLTPGAPHHLSSL
                     "
     exon            177..330
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            331..427
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            428..496
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            497..638
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            639..752
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            753..844
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            845..1015
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1170
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..3487
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3487
                     /gene="PAFAH2"
                     /gene_synonym="HSD-PLA2"
                     /note="major polyA site"
ORIGIN      
        1 aaacgtctgg ggctgcttct gaggaatcag cttgactggc cagcaagttc agctccggca
       61 agtcatttga ttcacccggt gatgaaatgg gggtcaacca gtctgtgggc tttccacctg
      121 tcacaggacc ccacctcgta ggctgtgggg atgtgatgga gggtcagaat ctccagggga
      181 gcttctttcg actcttctac ccctgccaaa aggcagagga gaccatggag cagcccctgt
      241 ggattccccg ctatgagtac tgcactggcc tggccgagta cctgcagttt aataagcgct
      301 gcgggggctt gctgttcaac ctggcggtgg gatcttgtcg cctgcctgtt agctggaatg
      361 gcccctttaa gacaaaggac tctggatacc ccttgatcat cttctcccat ggcctaggag
      421 ccttcaggac tttgtattca gccttctgca tggagctggc ctcacgtggc tttgtggttg
      481 ctgtgccaga gcacagggac cggtcagcgg caaccaccta tttctgcaag caggccccag
      541 aagagaacca gcccaccaat gaatcgctgc aggaggaatg gatccctttc cgtcgagttg
      601 aggaagggga gaaggaattt catgttcgga atccccaggt gcatcagcgg gtaagcgagt
      661 gtttacgggt gttgaagatc ctgcaagagg tcactgctgg gcagactgtc ttcaacatct
      721 tgcctggtgg cttggatctg atgactttga agggcaacat tgacatgagc cgtgtggctg
      781 tgatgggaca ttcatttgga ggggccacag ctattctggc tttggccaag gagacccaat
      841 ttcggtgtgc ggtggctctg gatgcttgga tgtttcctct ggaacgtgac ttttacccca
      901 aggcccgagg acctgtgttc tttatcaata ctgagaaatt ccagacaatg gagagtgtca
      961 atttgatgaa gaagatatgt gcccagcatg aacagtctag gatcataacc gttcttggtt
     1021 ctgttcatcg gagtcaaact gactttgctt ttgtgactgg caacttgatt ggtaaattct
     1081 tctccactga aacccgtggg agcctggacc cctatgaagg gcaggaggtt atggtacggg
     1141 ccatgttggc cttcctgcag aagcacctcg acctgaaaga agactataat caatggaaca
     1201 accttattga aggcattgga ccgtcgctca ccccaggggc cccccaccat ctgtccagcc
     1261 tgtaggcaca actggccatt tgtaaagtca cttcagccaa gttttcattt gggagctacc
     1321 caagggcacc catgagctcc tatcaagaag tgatcaacgt gacccctttt cacagattga
     1381 aaggtgtaat cacactgctg cttggataac tgggtacttt gatcttagat ttgatcttaa
     1441 aatcactttg ggactgggat cccttgctga ttgacaaaca gactttctgg gaccttgatg
     1501 gagtggggaa caagcagtag agtgggactg ggggagaccc aggccccggg ctgagcactg
     1561 tgaggcctgg atgtgaagac tcagcccagc gaagctcatt cccttacccc cggccagtgc
     1621 tgctgcttca gtggaagaga tgaagccaaa ggacagaatg aaaatcccta ccttcagaga
     1681 ctctagccca gcccaacacc atctcttcct acctctcagc cttctccctc cccagggcca
     1741 cttgttgaag tctgagcact ttatgtaaat ttctaggtgt gagccgtgat cacattttct
     1801 atttatttcc aagtcttctc attgtatgga acatagtact acttatactt acagtagtaa
     1861 gttatacttg tgagcccaca gagtggcaga cagcatggct ctcacagcac agggagaaaa
     1921 actgaggtac acagaggtac ctcagaagct ctggatgtct ttgggggttt tgctaagtgt
     1981 atcttgatag gaaacaacaa aagcaggttg agatggggaa gatgacagaa caacagtgtt
     2041 aaatggccat ttgcacaggc ctttgccaca acagagaagt agtttggtca gctaaaactc
     2101 agctgcagcc tggacagtag agcgagaccc catcttaaaa ataaagaagg ctgggcgtgg
     2161 tggctcatgc ctgtaatccc aacactttgg gaggccaagg caggcagatc acttaaggcc
     2221 aggagttcaa gaccacctgg ccaacatggt gaaaccccgt ctctactaaa aatacaaaaa
     2281 attagcctgg cgtaatggca ggcgcctata atcccagcta ctcaggaggc tgaagcagaa
     2341 gaatcacttg aacctaggag gcggaggttg cagtgagtca agatcgcgcc actgcactcc
     2401 agcctgggtg acagagcaag actctgtctt aaaaaaaaaa aaaagaaaaa gaaaaattta
     2461 aaataaataa aatttaaaaa cgcagaaggc cactcccaga gaggcctagg agtaccttgg
     2521 tcattttttc ttgcctcaaa tattataaac ctccaacttg ctcagaatca gggtgctttt
     2581 attgtctgtt ttctggctct gaggcccttg ccccaaattt aagtcagata cagactgaat
     2641 ttccattctg ttctgtttat aatatgaatg atattaatac tcttgtttgc ttaatgttat
     2701 gttggaattt tctgtttaat ctgcagtaga gcaagatgga gagccatctg gaattgactt
     2761 ccttctgtct ttcttctgtg ttcgcttact acttgggccc atgtgggaaa aaaactttaa
     2821 aaggaaggaa gtcttcttag ggatccagtt caaagctatt tgggggcatc aaactgaaac
     2881 tctcttctcc actactcaag acttcgtgcc tcagtccaag taggtaagag caaaagggaa
     2941 atgcatctct acacaatggc ccatggtcag ggccaccaga gcaagctggg cctagatctc
     3001 cacctgtcag tgactggcca ttctaagagg tgaaacagca gatcgttgag gctgtgaggg
     3061 aggatctttg aattcccatg agacatagca taagaagggt atcattaaag ccctgggctc
     3121 taaagttaca gagcctggtt ttgagtcctg gctccaccac tgccaactgg gtaaatttga
     3181 gcaaatggta ttctctttct tatctcagct cctgcgtttc taaaatgagg gtctgcgtta
     3241 tgaatagtta ggaggattaa gtgagaggat gtataaccca tgggttactt aaaagtatat
     3301 ttcctacctt ccaaacatga gatttttcta gttatagttt cttttctagc ttgattgctt
     3361 tgtggtcaga gaaaatgacc catatggttt ccatccttca aaatttgttg aaacttggtt
     3421 gttggcctag tatgagatca atgttgtaaa tgtgccatgt atgtttaaaa agactatata
     3481 ttctgaa
//
