LOCUS       NM_002572               4175 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens platelet activating factor acetylhydrolase 1b
            catalytic subunit 2 (PAFAH1B2), transcript variant 1, mRNA.
ACCESSION   NM_002572
VERSION     NM_002572.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Tanigawa,K., Kiriya,M., Hayashi,Y., Shinden,Y., Kijima,Y.,
            Natsugoe,S., Sumimoto,T., Morimoto-Kamata,R., Yui,S., Hama,K.,
            Yokoyama,K., Nakamura,Y., Suzuki,K., Nojiri,H., Inoue,K. and
            Karasawa,K.
  TITLE     Cathepsin G-induced malignant progression of MCF-7 cells involves
            suppression of PAF signaling through induced expression of PAFAH1B2
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1867 (8), 159164 (2022)
   PUBMED   35462067
  REMARK    GeneRIF: Cathepsin G-induced malignant progression of MCF-7 cells
            involves suppression of PAF signaling through induced expression of
            PAFAH1B2.
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4175)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 4175)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 4175)
  AUTHORS   Ma,C., Guo,Y., Zhang,Y., Duo,A., Jia,Y., Liu,C. and Li,B.
  TITLE     PAFAH1B2 is a HIF1a target gene and promotes metastasis in
            pancreatic cancer
  JOURNAL   Biochem Biophys Res Commun 501 (3), 654-660 (2018)
   PUBMED   29758199
  REMARK    GeneRIF: PAFAH1B2 overexpression in pancreatic ductal
            adenocarcinoma cells was directly mediated by HIF1a.
REFERENCE   6  (bases 1 to 4175)
  AUTHORS   Scott,B.T., Olson,N., Long,G.L. and Bovill,E.G.
  TITLE     Novel isoforms of intracellular platelet activating factor
            acetylhydrolase (PAFAH1b2) in human testis; encoded by
            alternatively spliced mRNAs
  JOURNAL   Prostaglandins Other Lipid Mediat 85 (3-4), 69-80 (2008)
   PUBMED   18155631
  REMARK    GeneRIF: splice variants of the Pafah1b2 gene transcript retain
            exons 1-5 and replace exon 6 with alternative exons derived from
            genomic sequence 3' to exon 6. Splice variants encode two proteins
            with different novel carboxy termini
REFERENCE   7  (bases 1 to 4175)
  AUTHORS   Sweeney,K.J., Clark,G.D., Prokscha,A., Dobyns,W.B. and Eichele,G.
  TITLE     Lissencephaly associated mutations suggest a requirement for the
            PAFAH1B heterotrimeric complex in brain development
  JOURNAL   Mech Dev 92 (2), 263-271 (2000)
   PUBMED   10727864
REFERENCE   8  (bases 1 to 4175)
  AUTHORS   Moro,F., Arrigo,G., Fogli,A., Bernard,L. and Carrozzo,R.
  TITLE     The beta and gamma subunits of the human platelet-activating factor
            acetyl hydrolase isoform Ib (PAFAH1B2 and PAFAH1B3) map to
            chromosome 11q23 and 19q13.1, respectively
  JOURNAL   Genomics 51 (1), 157-159 (1998)
   PUBMED   9693049
REFERENCE   9  (bases 1 to 4175)
  AUTHORS   Adachi,H., Tsujimoto,M., Hattori,M., Arai,H. and Inoue,K.
  TITLE     Differential tissue distribution of the beta- and gamma-subunits of
            human cytosolic platelet-activating factor acetylhydrolase (isoform
            I)
  JOURNAL   Biochem Biophys Res Commun 233 (1), 10-13 (1997)
   PUBMED   9144386
REFERENCE   10 (bases 1 to 4175)
  AUTHORS   Albrecht,U., Abu-Issa,R., Ratz,B., Hattori,M., Aoki,J., Arai,H.,
            Inoue,K. and Eichele,G.
  TITLE     Platelet-activating factor acetylhydrolase expression and activity
            suggest a link between neuronal migration and platelet-activating
            factor
  JOURNAL   Dev Biol 180 (2), 579-593 (1996)
   PUBMED   8954729
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP005018.1, D63390.1 and DQ836743.1.
            
            On Nov 23, 2018 this sequence version replaced NM_002572.3.
            
            Summary: Platelet-activating factor acetylhydrolase (PAFAH)
            inactivates platelet-activating factor (PAF) into acetate and
            LYSO-PAF. This gene encodes the beta subunit of PAFAH, the other
            subunits are alpha and gamma. Multiple alternatively spliced
            transcript variants have been described for this gene. [provided by
            RefSeq, Jan 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and it encodes the longest protein (isoform a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ836743.1, SRR3476690.714208.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000527958.6/ ENSP00000435289.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AP005018.1         7548-7584
            38-1228             D63390.1           1-1191
            1229-2613           AP005018.1         31360-32744
            2614-4175           DQ836743.1         2558-4119
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.3"
     gene            1..4175
                     /gene="PAFAH1B2"
                     /gene_synonym="HEL-S-303"
                     /note="platelet activating factor acetylhydrolase 1b
                     catalytic subunit 2"
                     /db_xref="GeneID:5049"
                     /db_xref="HGNC:HGNC:8575"
                     /db_xref="MIM:602508"
     exon            1..132
                     /gene="PAFAH1B2"
                     /gene_synonym="HEL-S-303"
                     /inference="alignment:Splign:2.1.0"
     exon            133..220
                     /gene="PAFAH1B2"
                     /gene_synonym="HEL-S-303"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..829
                     /gene="PAFAH1B2"
                     /gene_synonym="HEL-S-303"
                     /EC_number="3.1.1.47"
                     /note="isoform a is encoded by transcript variant 1;
                     PAF-AH1b alpha 2 subunit; epididymis secretory protein Li
                     303; intracellular platelet-activating factor
                     acetylhydrolase alpha 2 subunit; platelet-activating
                     factor acetylhydrolase IB subunit beta; PAFAH subunit
                     beta; PAF-AH subunit beta; PAF-AH 30 kDa subunit; PAF
                     acetylhydrolase 30 kDa subunit; platelet-activating factor
                     acetylhydrolase 1b, catalytic subunit 2 (30kDa);
                     epididymis secretory sperm binding protein;
                     platelet-activating factor acetylhydrolase IB subunit
                     alpha2"
                     /codon_start=1
                     /product="platelet-activating factor acetylhydrolase IB
                     subunit alpha2 isoform a"
                     /protein_id="NP_002563.1"
                     /db_xref="CCDS:CCDS8380.1"
                     /db_xref="GeneID:5049"
                     /db_xref="HGNC:HGNC:8575"
                     /db_xref="MIM:602508"
                     /translation="MSQGDSNPAAIPHAAEDIQGDDRWMSQHNRFVLDCKDKEPDVLF
                     VGDSMVQLMQQYEIWRELFSPLHALNFGIGGDTTRHVLWRLKNGELENIKPKVIVVWV
                     GTNNHENTAEEVAGGIEAIVQLINTRQPQAKIIVLGLLPRGEKPNPLRQKNAKVNQLL
                     KVSLPKLANVQLLDTDGGFVHSDGAISCHDMFDFLHLTGGGYAKICKPLHELIMQLLE
                     ETPEEKQTTIA"
     exon            221..310
                     /gene="PAFAH1B2"
                     /gene_synonym="HEL-S-303"
                     /inference="alignment:Splign:2.1.0"
     exon            311..427
                     /gene="PAFAH1B2"
                     /gene_synonym="HEL-S-303"
                     /inference="alignment:Splign:2.1.0"
     exon            428..550
                     /gene="PAFAH1B2"
                     /gene_synonym="HEL-S-303"
                     /inference="alignment:Splign:2.1.0"
     exon            551..4175
                     /gene="PAFAH1B2"
                     /gene_synonym="HEL-S-303"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4152..4157
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAFAH1B2"
                     /gene_synonym="HEL-S-303"
                     /note="hexamer: TATAAA"
     polyA_site      4175
                     /gene="PAFAH1B2"
                     /gene_synonym="HEL-S-303"
                     /note="major polyA site"
ORIGIN      
        1 ggaggagctg ctgctggtgc tggggccgga ggagggacgc gccggagcgg gaccgacggg
       61 accgagcgag cgaccgacgc gccacccgcc gacgcctcag ccgcttgggg cccgcacgga
      121 ccctctactt cagtgtagaa tgagccaagg agactcaaac ccagcagcta ttccgcatgc
      181 agcagaagat attcaaggag atgaccgatg gatgtctcag cacaacagat ttgttttgga
      241 ctgtaaagac aaagagcctg atgtactgtt cgtgggagac tccatggtgc agttaatgca
      301 gcaatatgag atatggcgag agcttttttc cccacttcat gcactgaatt ttggaattgg
      361 gggagataca acaagacatg ttttgtggag actaaagaat ggagaactgg agaatattaa
      421 gcctaaggtc attgttgtct gggtaggaac aaataaccac gaaaatacag cagaagaagt
      481 agcaggtggg atcgaggcca ttgtacaact tatcaacaca aggcagccac aggccaaaat
      541 cattgtattg ggtttgttac ctcgaggtga gaaacccaat cctttgaggc aaaagaacgc
      601 caaggtgaac caactcctca aggtttcgct gccgaagctt gccaacgtgc agctcctgga
      661 taccgacggg ggttttgtgc actcggacgg tgccatctcc tgccacgaca tgtttgattt
      721 tctgcatctg acaggagggg gctatgcaaa gatctgcaaa cccctgcatg aactgatcat
      781 gcagttgttg gaggaaacac ctgaggagaa acaaaccacc attgcctgac tggctcttat
      841 cagtgttaat agcatctcag cttcctcaga tcagttctat cactggcact acagaatcct
      901 tctctttctt aaggcacttt gcattgtaga atgttcctgg atgttcatat ctagtgtttg
      961 aaggggagga gggatttaaa ctggtcctgt acatagaagg tttgtttgac agaggagaaa
     1021 aattagccaa ggaagattgt tgtttaaatt catttgaaac cagaagggga ctttttagtt
     1081 gtatgtgtaa cacattcatt gaattattat cactgttttc ttgggacaac atcaagccta
     1141 aatactgaac aatatgaaga ttcttttctt ggcctttctg tggattatgt catatataat
     1201 aattatcaga atcattctac ttggctttaa aacatgtttt ctccaatttt tttaaggttc
     1261 ataatttagc cttttgtttt tatgttgctt agattcttat gtatactgaa tattttatta
     1321 acatgtagca tcaggttgaa catgcttgtc attgatatat ggaagatgct atagttagaa
     1381 gtgaatttgt tctgctttct taatcttttc catgcttagc agtgaaaaac aggttttgcc
     1441 ccagtagagg gattctttgg agggtattat tttttatgct gctgaatatc atgtctataa
     1501 tagacccgtg catgcagcct ttcctcctta ttccccttca tgcccccctt tccccttcat
     1561 tccccccgcc accccgtttt tttttttttt tttttttttt tggttcttgt tgacattaca
     1621 agcttttaac acatttttga cctaggagcc ctgttgctgg aagtatagtc tccagccagt
     1681 tactctcatg atagtactgc tataaaactc attcttgtgt ggtgttctgt gctatagatt
     1741 ctgtgtattg ctgttcatat tcggagttct ggttttgttt ttcccttaaa acctgttaac
     1801 agtttttttt gggggtgggg ggattcagaa ctcttgtttc ccattccata gcacctgaca
     1861 ttatttcaag ttttataata tcttaaggtg tatattttat tttttttatt ggcttagttg
     1921 ttttttgttt tgttttgttt gagatggagt ttcactgttg cccaggctgg ggtgcaatgg
     1981 tgtgatcttg gctcactgca gcctccacct cccgagttca aatgattctc ctgcctcagc
     2041 ttcctgagta gctgggatta caggtgcatg ccaccatgcc cggctaattt ttatattttt
     2101 attagagacg ggatttcgcc atgttaacca ggctggtctc gaactcctga cctcaggtga
     2161 tctgcccgcc ttggcctccc aaagtgctgg gattacaggc atgagccacc gtgcctggcc
     2221 ttattggctt agtttttaaa ttatcctcca aaaattttgg gcctttttct gtggggaaac
     2281 aagtgaagct gctcttcagc atagacacta cctttatccc atcattttag taaaaactag
     2341 gtttgtttca cttctgaggt gtcttattaa tgtacttcat ctgagaattt gttgatctta
     2401 atgttcgagc tatataagaa ctgccattaa aaaaaatggg ataatagatg attttatcag
     2461 tatacctgtg gaatatgtac aaactggatc tatagatatt ttgaactgga ccaggtgggt
     2521 attgaagtaa cccatcaaaa tatgctctgc agtgattccg cttaatgttt aaattcagta
     2581 acgtacttga aaggcaaatt tcagtgcttt tgtatgttgg aggagggctt actgatgcgt
     2641 gctaagaccg atttctgatt gagggatgaa ccttgggctc atttttttct tgtgaagtct
     2701 ctttctagaa aattttttgg ttttgttctt tttaaaaaat acatactttt ttgaatgtat
     2761 catgtcttca ttaacaacag aaaatccaca tggtgtttac taaacttgtt tacgacatta
     2821 aaaatttcct ttttattttt agtagcccag gttgagtttt tcacaagaga tttttttctt
     2881 agctgaggta tagttgtata gcaagaagaa ttaagccaga tttttgtgtg tggaaagaca
     2941 gttttctatc cacgtctttt tctgtttgtc agaaggtggg agtatggtcc aaataaatcc
     3001 attaggttac tcctgcagca tgcgctttta gcttctctct tgactgagga tcaaatatcc
     3061 ctttgtgagc tggccctcag ctcctttgct catgtgtaca aacctcagat gttactacat
     3121 tttatatcta ccagagctat tcaagcaata gtatttgaac cactagcctt ttaaataaaa
     3181 ttctgcccca ttactgatgt gcagatattg agttcacttt cattttttgc cagatttctt
     3241 tgcactactt taggtaaaaa tagttaatct atttttcttt gacatcctag tttgcgtcag
     3301 tgacagaact tactgcttag tctttgtact ttttaaaaaa tctataaatt taatgcactg
     3361 tccaagtgaa atgtcctagt tgtcattgtg attaaggggc caactttcca ggcagctagc
     3421 agagatacta ttctcttcct ctcccagcaa atttgtattc cttcgcccac gcattcctgc
     3481 tatactagat ggcagccagt gatggaacta taaagatgtc tgtggtcata tgttgaatgt
     3541 ggcagcttga agatgtactg ccacgggtga tctagggcag gctgtcttcc agtccatgtg
     3601 ttctcggtcg ccgtagacag cgctctggct accaccgtga ggctacttga actgtcaggg
     3661 gcatctgcct aaaccagaat cttttgtcag aaaccttaac ccaacaaaac aaatcttgag
     3721 tagctcatgc ccggctctta ggaattttgt ctgtttaaaa aaaaaaaaaa aaaaaaagtc
     3781 caacttactt tattttattt ttttaaccta gtcactgttt acaattgtat gctaaagcct
     3841 gaaatattgt ctgtgctgtg gtgtatgagc attgccaact ttatatttat tgcagtgaag
     3901 aagaaactaa aaatatatgg aaatgaggag catgtccaag ctcctaaatc cgtgtgggtg
     3961 catgtgggag aagtgagtta gggcctcttg aaaggaggct ttttggagag gggtccccca
     4021 ggtttcttgg tgttcctgct tggggatcac tgctgctagc tgactggacc tccccattgg
     4081 aagtttgtga ttttgctttg gcaaagtttc attgactagt agaactcatt ctgttttagt
     4141 gtatatttca atataaatgt aaacattttg ctcaa
//
