LOCUS       NM_019088               2200 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens PAF1 homolog, Paf1/RNA polymerase II complex component
            (PAF1), transcript variant 1, mRNA.
ACCESSION   NM_019088
VERSION     NM_019088.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Aoki,K., Nitta,A. and Igarashi,A.
  TITLE     NELF and PAF1C complexes are core transcriptional machineries
            controlling colon cancer stemness
  JOURNAL   Oncogene 43 (8), 566-577 (2024)
   PUBMED   38182897
  REMARK    GeneRIF: NELF and PAF1C complexes are core transcriptional
            machineries controlling colon cancer stemness.
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Zhang,Z., Chen,Y., Guo,Y., Shen,H., Wang,J. and Chen,H.
  TITLE     RFX2 promotes tumor cell stemness through epigenetic regulation of
            PAF1 in spinal ependymoma
  JOURNAL   J Neurooncol 165 (3), 487-497 (2023)
   PUBMED   38057505
  REMARK    GeneRIF: RFX2 promotes tumor cell stemness through epigenetic
            regulation of PAF1 in spinal ependymoma.
REFERENCE   3  (bases 1 to 2200)
  AUTHORS   Zhang,B., Zhao,B., Han,S. and Chen,S.
  TITLE     CNOT4 suppresses nonsmall cell lung cancer progression by promoting
            the degradation of PAF1
  JOURNAL   Mol Carcinog 62 (10), 1563-1571 (2023)
   PUBMED   37493105
  REMARK    GeneRIF: CNOT4 suppresses nonsmall cell lung cancer progression by
            promoting the degradation of PAF1.
REFERENCE   4  (bases 1 to 2200)
  AUTHORS   Liu,X., Liu,X., Du,Y., Zou,D., Tian,C., Li,Y., Lan,X., David,C.J.,
            Sun,Q. and Chen,M.
  TITLE     Aberrant accumulation of Kras-dependent pervasive transcripts
            during tumor progression renders cancer cells dependent on PAF1
            expression
  JOURNAL   Cell Rep 42 (8), 112979 (2023)
   PUBMED   37572321
  REMARK    GeneRIF: Aberrant accumulation of Kras-dependent pervasive
            transcripts during tumor progression renders cancer cells dependent
            on PAF1 expression.
REFERENCE   5  (bases 1 to 2200)
  AUTHORS   Rauth,S., Ganguly,K., Atri,P., Parte,S., Nimmakayala,R.K.,
            Varadharaj,V., Nallasamy,P., Vengoji,R., Ogunleye,A.O.,
            Lakshmanan,I., Chirravuri,R., Bessho,M., Cox,J.L., Foster,J.M.,
            Talmon,G.A., Bessho,T., Ganti,A.K., Batra,S.K. and Ponnusamy,M.P.
  TITLE     Elevated PAF1-RAD52 axis confers chemoresistance to human cancers
  JOURNAL   Cell Rep 42 (2), 112043 (2023)
   PUBMED   36709426
  REMARK    GeneRIF: Elevated PAF1-RAD52 axis confers chemoresistance to human
            cancers.
REFERENCE   6  (bases 1 to 2200)
  AUTHORS   Moniaux,N., Nemos,C., Schmied,B.M., Chauhan,S.C., Deb,S.,
            Morikane,K., Choudhury,A., Vanlith,M., Sutherlin,M., Sikela,J.M.,
            Hollingsworth,M.A. and Batra,S.K.
  TITLE     The human homologue of the RNA polymerase II-associated factor 1
            (hPaf1), localized on the 19q13 amplicon, is associated with
            tumorigenesis
  JOURNAL   Oncogene 25 (23), 3247-3257 (2006)
   PUBMED   16491129
  REMARK    GeneRIF: Overexpression of PD2/hPaf1 is involved in cancer
            development.
REFERENCE   7  (bases 1 to 2200)
  AUTHORS   Zhu,B., Zheng,Y., Pham,A.D., Mandal,S.S., Erdjument-Bromage,H.,
            Tempst,P. and Reinberg,D.
  TITLE     Monoubiquitination of human histone H2B: the factors involved and
            their roles in HOX gene regulation
  JOURNAL   Mol Cell 20 (4), 601-611 (2005)
   PUBMED   16307923
  REMARK    GeneRIF: Formation of trimeric complex UbcH6 and RNF20/40 with PAF
            stimulates histone 2B monoubiquitination activity in vitro
REFERENCE   8  (bases 1 to 2200)
  AUTHORS   Zhu,B., Mandal,S.S., Pham,A.D., Zheng,Y., Erdjument-Bromage,H.,
            Batra,S.K., Tempst,P. and Reinberg,D.
  TITLE     The human PAF complex coordinates transcription with events
            downstream of RNA synthesis
  JOURNAL   Genes Dev 19 (14), 1668-1673 (2005)
   PUBMED   16024656
REFERENCE   9  (bases 1 to 2200)
  AUTHORS   Yart,A., Gstaiger,M., Wirbelauer,C., Pecnik,M., Anastasiou,D.,
            Hess,D. and Krek,W.
  TITLE     The HRPT2 tumor suppressor gene product parafibromin associates
            with human PAF1 and RNA polymerase II
  JOURNAL   Mol Cell Biol 25 (12), 5052-5060 (2005)
   PUBMED   15923622
REFERENCE   10 (bases 1 to 2200)
  AUTHORS   Rozenblatt-Rosen,O., Hughes,C.M., Nannepaga,S.J., Shanmugam,K.S.,
            Copeland,T.D., Guszczynski,T., Resau,J.H. and Meyerson,M.
  TITLE     The parafibromin tumor suppressor protein is part of a human Paf1
            complex
  JOURNAL   Mol Cell Biol 25 (2), 612-620 (2005)
   PUBMED   15632063
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA821359.1 and BC000017.2.
            
            On Nov 22, 2018 this sequence version replaced NM_019088.3.
            
            Summary: This gene encodes a subunit of the polymerase associated
            factor (PAF1) complex. The PAF1 complex interacts with RNA
            polymerase II and plays a role in transcription elongation as well
            as histone modifications including ubiquitylation and methylation.
            Alternatively spliced transcript variants encoding multiple
            isoforms have been observed for this gene. [provided by RefSeq, Feb
            2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC000017.2, SRR14038194.4021007.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000221265.8/ ENSP00000221265.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                DA821359.1         90-156
            68-2200             BC000017.2         1-2133
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.2"
     gene            1..2200
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /note="PAF1 homolog, Paf1/RNA polymerase II complex
                     component"
                     /db_xref="GeneID:54623"
                     /db_xref="HGNC:HGNC:25459"
                     /db_xref="MIM:610506"
     exon            1..289
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             243..1838
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /note="isoform 1 is encoded by transcript variant 1; RNA
                     polymerase II-associated factor 1 homolog; pancreatic
                     differentiation protein 2; Paf1, RNA polymerase II
                     associated factor, homolog"
                     /codon_start=1
                     /product="RNA polymerase II-associated factor 1 homolog
                     isoform 1"
                     /protein_id="NP_061961.2"
                     /db_xref="CCDS:CCDS12533.1"
                     /db_xref="GeneID:54623"
                     /db_xref="HGNC:HGNC:25459"
                     /db_xref="MIM:610506"
                     /translation="MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPF
                     DPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDP
                     ADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVK
                     QQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPC
                     AQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQE
                     EEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSK
                     RRAKAGVQSGTNALLVVKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMETEEKEAG
                     GSDEEQEKGSSSEKEGSEDEHSGSESEREEGDRDEASDKSGSGEDESSEDEARAARDK
                     EEIFGSDADSEDDADSDDEDRGQAQGGSDNDSDSGSNGGGQRSRSHSRSASPFPSGSE
                     HSAQEDGSEAAASDSSEADSDSD"
     exon            290..319
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            320..412
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            413..534
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            535..601
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            602..703
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            704..809
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            810..878
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            879..982
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1099
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1228
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1334
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1425
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..2200
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1957..1962
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /note="hexamer: AATAAA"
     polyA_site      1977
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /note="major polyA site"
     regulatory      2176..2181
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /note="hexamer: ATTAAA"
     regulatory      2180..2185
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
                     /note="hexamer: AACAAG"
     polyA_site      2190
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
     polyA_site      2200
                     /gene="PAF1"
                     /gene_synonym="F23149_1; PD2"
ORIGIN      
        1 agcgtcttgg cggcagttgg tggaaccgga gcttcgagtc cgtccccggt gctgcctgcg
       61 cgttcacctg agtctcgctg gagctcttct cgcccgccca cctcatctca acccactttc
      121 cgcggggagc ggcgccaagc tgggccttcc tcggatcagg cgtcccctga agtcggcacg
      181 cccctctgcg tcccccttcg gtcccgctag gaccccgtcc gggctgccgt cgcctcgtcg
      241 ctatggcgcc caccatccag acccaggccc agcgggagga tggccacagg cccaattccc
      301 accggactct gcctgagagg tctggagtgg tctgccgagt caagtactgc aatagcctcc
      361 ctgatatccc cttcgacccc aagttcatca cctacccctt cgaccagaac aggttcgtcc
      421 agtacaaagc cacttccttg gagaaacagc acaaacatga cctcctgact gagccagacc
      481 tgggggtcac catcgatctc atcaatcctg acacctaccg catcgacccc aatgttcttc
      541 tagatccagc tgatgagaaa cttttggaag aggagattca ggcccccacc agctccaaga
      601 gatcccagca gcacgcgaag gtggtgccat ggatgcgaaa gacagagtac atctccactg
      661 agttcaaccg ttatggcatc tccaatgaga agcctgaggt caagattggg gtttctgtga
      721 agcagcagtt taccgaggaa gaaatataca aagacaggga tagccagatc acagccattg
      781 agaagacttt tgaggatgcc cagaaatcaa tctcacagca ttacagcaaa ccccgagtca
      841 caccggtgga ggtcatgcct gtcttcccag actttaagat gtggatcaat ccatgtgctc
      901 aggtgatctt tgactcagac ccagccccca aggacacgag tggtgcagct gcgttggaga
      961 tgatgtctca ggccatgatt aggggcatga tggatgagga agggaaccag tttgtggcct
     1021 atttcctgcc tgtagaagag acgttgaaga aacgaaagcg ggaccaggag gaggagatgg
     1081 actatgcacc agatgatgtg tatgactaca aaattgctcg ggagtacaac tggaacgtga
     1141 agaacaaagc tagcaagggc tatgaggaaa actacttctt catcttccga gagggtgacg
     1201 gggtttacta caatgagttg gaaaccaggg tccgccttag taagcgccgg gccaaggctg
     1261 gggttcagtc aggcaccaac gccctgcttg tggtcaaaca tcgggacatg aatgagaagg
     1321 aactggaagc tcaggaggca cggaaggccc agctagaaaa ccacgaaccg gaggaggaag
     1381 aggaagagga gatggagaca gaagagaaag aagctggggg ctcagatgag gagcaggaga
     1441 agggcagcag cagtgagaag gagggcagtg aagatgagca ctcgggcagc gagagtgaac
     1501 gggaggaagg tgacagggac gaggccagtg acaagagtgg cagtggtgag gacgagagca
     1561 gcgaggatga ggcccgggct gcccgtgaca aagaggagat ctttggcagt gatgctgatt
     1621 ctgaggacga tgccgactct gatgatgagg acagaggaca ggcccaaggt ggcagtgaca
     1681 atgattcaga cagcggcagc aatgggggtg gccagcggag ccggagccac agccgcagcg
     1741 ccagtccctt ccccagtggc agcgagcact cggcccagga ggatggcagt gaagctgcag
     1801 cttctgattc cagtgaagct gatagtgaca gtgactgagt cccagggcat tcagggctgg
     1861 ttcagacacc attattgtga gcagcaaagc acttttctag tggtctgttt gtgagccttt
     1921 cacttgtttg ttccccaccc ccaaaccttt gctgttaata aagtcaactt ctctttactt
     1981 cccctcccag cccctatgat gtcattcagc ccagcacata cctcccgcaa cccccaggtg
     2041 ctcaaggatc ttggcttgag gcttagaaac acaggccaga ggcagggcca atggtttccc
     2101 acaaaatgta gcacaacgaa ggtcagaatt cgtttttttt gtttgttttt tttttttcca
     2161 aaataagccc agaccattaa acaagtgaaa ctccaacaaa
//
