LOCUS       NM_001184970            3251 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens protein kinase C and casein kinase substrate in
            neurons 2 (PACSIN2), transcript variant 1, mRNA.
ACCESSION   NM_001184970
VERSION     NM_001184970.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Wint,H., Li,J., Abe,T., Yamada,H., Higaki,T., Nasu,Y., Watanabe,M.,
            Takei,K. and Takeda,T.
  TITLE     Pacsin 2-dependent N-cadherin internalization regulates the
            migration behaviour of malignant cancer cells
  JOURNAL   J Cell Sci 136 (10) (2023)
   PUBMED   37132654
  REMARK    GeneRIF: Pacsin 2-dependent N-cadherin internalization regulates
            the migration behaviour of malignant cancer cells.
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Zudeh,G., Franca,R., Lucafo,M., Bonten,E.J., Bramuzzo,M.,
            Sgarra,R., Lagatolla,C., Franzin,M., Evans,W.E., Decorti,G. and
            Stocco,G.
  TITLE     PACSIN2 as a modulator of autophagy and mercaptopurine
            cytotoxicity: mechanisms in lymphoid and intestinal cells
  JOURNAL   Life Sci Alliance 6 (3), e202201610 (2023)
   PUBMED   36596605
  REMARK    GeneRIF: PACSIN2 as a modulator of autophagy and mercaptopurine
            cytotoxicity: mechanisms in lymphoid and intestinal cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3251)
  AUTHORS   Park,K., Yoo,H.S., Oh,C.K., Lee,J.R., Chung,H.J., Kim,H.N.,
            Kim,S.H., Kee,K.M., Kim,T.Y., Kim,M., Kim,B.G., Ra,J.S., Myung,K.,
            Kim,H., Han,S.H., Seo,M.D., Lee,Y. and Kim,D.W.
  TITLE     Reciprocal interactions among Cobll1, PACSIN2, and SH3BP1 regulate
            drug resistance in chronic myeloid leukemia
  JOURNAL   Cancer Med 11 (21), 4005-4020 (2022)
   PUBMED   35352878
  REMARK    GeneRIF: Reciprocal interactions among Cobll1, PACSIN2, and SH3BP1
            regulate drug resistance in chronic myeloid leukemia.
REFERENCE   4  (bases 1 to 3251)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 3251)
  AUTHORS   Li,J., Fujise,K., Wint,H., Senju,Y., Suetsugu,S., Yamada,H.,
            Takei,K. and Takeda,T.
  TITLE     Dynamin 2 and BAR domain protein pacsin 2 cooperatively regulate
            formation and maturation of podosomes
  JOURNAL   Biochem Biophys Res Commun 571, 145-151 (2021)
   PUBMED   34325130
  REMARK    GeneRIF: Dynamin 2 and BAR domain protein pacsin 2 cooperatively
            regulate formation and maturation of podosomes.
REFERENCE   6  (bases 1 to 3251)
  AUTHORS   Kessels,M.M. and Qualmann,B.
  TITLE     The syndapin protein family: linking membrane trafficking with the
            cytoskeleton
  JOURNAL   J Cell Sci 117 (Pt 15), 3077-3086 (2004)
   PUBMED   15226389
  REMARK    Review article
REFERENCE   7  (bases 1 to 3251)
  AUTHORS   Ghadimi,M.P., Sanzenbacher,R., Thiede,B., Wenzel,J., Jing,Q.,
            Plomann,M., Borkhardt,A., Kabelitz,D. and Janssen,O.
  TITLE     Identification of interaction partners of the cytosolic polyproline
            region of CD95 ligand (CD178)
  JOURNAL   FEBS Lett 519 (1-3), 50-58 (2002)
   PUBMED   12023017
REFERENCE   8  (bases 1 to 3251)
  AUTHORS   Sumoy,L., Pluvinet,R., Andreu,N., Estivill,X. and Escarceller,M.
  TITLE     PACSIN 3 is a novel SH3 domain cytoplasmic adapter protein of the
            pacsin-syndapin-FAP52 gene family
  JOURNAL   Gene 262 (1-2), 199-205 (2001)
   PUBMED   11179684
REFERENCE   9  (bases 1 to 3251)
  AUTHORS   Modregger,J., Ritter,B., Witter,B., Paulsson,M. and Plomann,M.
  TITLE     All three PACSIN isoforms bind to endocytic proteins and inhibit
            endocytosis
  JOURNAL   J Cell Sci 113 Pt 24, 4511-4521 (2000)
   PUBMED   11082044
REFERENCE   10 (bases 1 to 3251)
  AUTHORS   Ritter,B., Modregger,J., Paulsson,M. and Plomann,M.
  TITLE     PACSIN 2, a novel member of the PACSIN family of cytoplasmic
            adapter proteins
  JOURNAL   FEBS Lett 454 (3), 356-362 (1999)
   PUBMED   10431838
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY009996.1, AF128536.1 and
            BC008037.2.
            
            On Nov 23, 2018 this sequence version replaced NM_001184970.2.
            
            Summary: This gene is a member of the protein kinase C and casein
            kinase substrate in neurons family. The encoded protein is involved
            in linking the actin cytoskeleton with vesicle formation by
            regulating tubulin polymerization. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (1) encodes isoform A. Both
            variants 1 and 2 encode the same protein (isoform A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1013881.1, AF128536.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263246.8/ ENSP00000263246.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 HY009996.1         27-30
            5-412               AF128536.1         7-414
            413-1345            BC008037.2         429-1361
            1346-3251           AF128536.1         1348-3253
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.2"
     gene            1..3251
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /note="protein kinase C and casein kinase substrate in
                     neurons 2"
                     /db_xref="GeneID:11252"
                     /db_xref="HGNC:HGNC:8571"
                     /db_xref="MIM:604960"
     exon            1..129
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /inference="alignment:Splign:2.1.0"
     exon            130..266
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..1667
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /note="isoform A is encoded by transcript variant 1;
                     syndapin2; syndapin-II; syndapin-2; cytoplasmic
                     phosphoprotein PACSIN2"
                     /codon_start=1
                     /product="protein kinase C and casein kinase substrate in
                     neurons protein 2 isoform A"
                     /protein_id="NP_001171899.1"
                     /db_xref="CCDS:CCDS43023.1"
                     /db_xref="GeneID:11252"
                     /db_xref="HGNC:HGNC:8571"
                     /db_xref="MIM:604960"
                     /translation="MSVTYDDSVGVEVSSDSFWEVGNYKRTVKRIDDGHRLCSDLMNC
                     LHERARIEKAYAQQLTEWARRWRQLVEKGPQYGTVEKAWMAFMSEAERVSELHLEVKA
                     SLMNDDFEKIKNWQKEAFHKQMMGGFKETKEAEDGFRKAQKPWAKKLKEVEAAKKAHH
                     AACKEEKLAISREANSKADPSLNPEQLKKLQDKIEKCKQDVLKTKEKYEKSLKELDQG
                     TPQYMENMEQVFEQCQQFEEKRLRFFREVLLEVQKHLDLSNVAGYKAIYHDLEQSIRA
                     ADAVEDLRWFRANHGPGMAMNWPQFEEWSADLNRTLSRREKKKATDGVTLTGINQTGD
                     QSLPSKPSSTLNVPSNPAQSAQSQSSYNPFEDEDDTGSTVSEKDDTKAKNVSSYEKTQ
                     SYPTDWSDDESNNPFSSTDANGDSNPFDDDATSGTEVRVRALYDYEGQEHDELSFKAG
                     DELTKMEDEDEQGWCKGRLDNGQVGLYPANYVEAIQ"
     exon            267..423
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /inference="alignment:Splign:2.1.0"
     exon            424..659
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /inference="alignment:Splign:2.1.0"
     exon            660..815
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /inference="alignment:Splign:2.1.0"
     exon            816..991
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1112
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1234
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1357
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1554
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..3251
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3230..3235
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /note="hexamer: AATAAA"
     polyA_site      3251
                     /gene="PACSIN2"
                     /gene_synonym="SDPII"
                     /note="major polyA site"
ORIGIN      
        1 agaagcggcc gcaggggtct gggcagggct gggcagtgct gccggagcaa aagcggtagc
       61 gggagcccgg ccggagctgg gtctggagac gccgtggcag cctgaacgga gtgtgcgacg
      121 gattgggagg tttgtctaca gattttgagc gttcgaagtt gacccctgac taagtatact
      181 ttgctgctcc ctcagccttt gaaaaaatgt ctgtcacata tgatgattcc gttggagtag
      241 aagtgtccag cgacagcttc tgggaggtcg ggaactacaa gcggactgtg aagcggatcg
      301 acgatggcca ccgcctgtgc agcgacctca tgaactgcct gcatgagcgg gcgcgcatcg
      361 agaaggcgta tgcgcagcag ctcactgagt gggcccggcg ctggaggcag ctcgtggaga
      421 aagggcccca gtacgggacc gtggagaagg cctggatggc cttcatgtcc gaggcagaga
      481 gggtgagcga gctgcacctc gaggtgaagg cctcactgat gaacgatgac ttcgagaaga
      541 tcaagaactg gcagaaggaa gcctttcaca agcagatgat gggcggcttc aaggagacca
      601 aggaagctga ggacggcttt cggaaggcac agaagccctg ggccaagaag ctgaaagagg
      661 tagaagcagc aaagaaagcc caccatgcag cgtgcaaaga ggagaagctg gctatctcac
      721 gagaagccaa cagcaaggca gacccatccc tcaaccctga acagctcaag aaattgcaag
      781 acaaaataga aaagtgcaag caagatgttc ttaagaccaa agagaagtat gagaagtccc
      841 tgaaggaact cgaccagggc acaccccagt acatggagaa catggagcag gtgtttgagc
      901 agtgccagca gttcgaggag aaacgccttc gcttcttccg ggaggttctg ctggaggttc
      961 agaagcacct agacctgtcc aatgtggctg gctacaaagc catttaccat gacctggagc
     1021 agagcatcag agcagctgat gcagtggagg acctgaggtg gttccgagcc aatcacgggc
     1081 cgggcatggc catgaactgg ccgcagtttg aggagtggtc cgcagacctg aatcgaaccc
     1141 tcagccggag agagaagaag aaggccactg acggcgtcac cctgacgggc atcaaccaga
     1201 caggcgacca gtctctgccg agtaagccca gcagcaccct taatgtcccg agcaaccccg
     1261 cccagtctgc gcagtcacag tccagctaca accccttcga ggatgaggac gacacgggca
     1321 gcaccgtcag tgagaaggac gacactaagg ccaaaaatgt gagcagctac gagaagaccc
     1381 agagctatcc caccgactgg tcagacgatg agtctaacaa ccccttctcc tccacggatg
     1441 ccaatgggga ctcgaatcca ttcgacgacg acgccacctc ggggacggaa gtgcgagtcc
     1501 gggccctgta tgactatgag gggcaggagc atgatgagct gagcttcaag gctggggatg
     1561 agctgaccaa gatggaggac gaggatgagc agggctggtg caagggacgc ttggacaacg
     1621 ggcaagttgg cctatacccg gcaaattatg tggaggcgat ccagtgatga gtcggggaca
     1681 ggccagcggg gggacggagg cggcgggccc aggagcctca gccagccacg tgggcatcca
     1741 ctccttttcc tgcaagagat gatggttcca ttgctcttgg cttcatggtg ttcctggaag
     1801 gcagatgagc tggtcatttc gcctgggact cggcaccttt ccgagtgcag ctggagggat
     1861 ctgagcgcag gaagacgcag aacaacagaa atagccgccc ctccccgccc actgtgcctg
     1921 ttggcctatc atagatctct atgttcttga ctttgtctct cctttccgag tcaatggtgg
     1981 gttacactga tcttgttcca ctgattactc tctctgacga gtccatcacc tgcaacttaa
     2041 atgaacaagc ttacatccca ttttgagtga agattttgag gtttttaatt taaaggctgt
     2101 gtacagttat acttttttat acacctgttc atttctactt aaattatggc acagattgat
     2161 gcgcaccagt cttgaggaaa cgatctccct attcccttac cctgttactc agccacgccg
     2221 tgtgtaggct tagcctcagg tggcagatgt ttgaggaaag gaattatgcc aggaaggtgg
     2281 gaccgggtta tggtcgggtt tctattggga atgctctttg tgcttttggg catctgaatg
     2341 gaagctttac atagaacctt aggtagaact cccccaaatc gccatattta aaaattattt
     2401 tcactctatt cttgcttaaa actgtactct tttgcaaatt aacaatttta tcactgattc
     2461 agagttaaaa agaagactaa cttttcaagc aaatgcatct gtaaagatgc tttagattag
     2521 actgtcatgt ctcagtgtct atctgtatat attatttgat attcagagaa tctaaagcac
     2581 tcgtctactg ttttaatgag atttaacagc ttttaacagt gagtttcgtt tgtaaactgc
     2641 ttgaagtctg tggcattcag gcacacgtct ggctggccgg ctgggtctcc tcccgggctc
     2701 agtgggcctg gggcctctct gacgtggtgc ctgctggagg gaggctcgtc gtcaccagct
     2761 gactgctggt ccggcttctg accggccttt gtcctggctc cgtagcagaa cactgtaaaa
     2821 gtgcccgcgt ctttgcagta gttgcagatt tcagtcgtcg tgttacttgt gcacaaacag
     2881 aagctgggtc ttacccgcag cacgagtgtc tcgggctgcc cggagtcgcc cgggagcagg
     2941 tgctgcagcc agagttacgc gggggccacg cgggccggcg ggggtggggg gaacgtgggg
     3001 gaacctgtgt ttcacgtgac tcagcagtgc ccgccgccgt caccagctat gcattcactc
     3061 cgtttccagt gagcagatgt cttgcttgga aagtggacct gtgtctgtgt ctgtcctgag
     3121 aacttaccag cagaaatcct catttctgtg ctacggattt accaaaaatt gtcaagtctt
     3181 tttcagttta acagttcctt tacatgtgta gtatttgagg aaaaaaatca ataaacagtt
     3241 gatctcgtgc a
//
