LOCUS       NM_182904               2255 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens prolyl 4-hydroxylase subunit alpha 3 (P4HA3),
            transcript variant 1, mRNA.
ACCESSION   NM_182904
VERSION     NM_182904.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Zhang,Z., Zhang,Y. and Zhang,R.
  TITLE     P4HA3 promotes clear cell renal cell carcinoma progression via the
            PI3K/AKT/GSK3beta pathway
  JOURNAL   Med Oncol 40 (2), 70 (2023)
   PUBMED   36588128
  REMARK    GeneRIF: P4HA3 promotes clear cell renal cell carcinoma progression
            via the PI3K/AKT/GSK3beta pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Zhou,H., Huang,S., Shao,C., Zou,J., Zhou,A., Yu,J. and Xu,C.
  TITLE     miR-1266-3p Suppresses Epithelial-Mesenchymal Transition in Colon
            Cancer by Targeting P4HA3
  JOURNAL   Anal Cell Pathol (Amst) 2022, 1542117 (2022)
   PUBMED   35433237
  REMARK    GeneRIF: miR-1266-3p Suppresses Epithelial-Mesenchymal Transition
            in Colon Cancer by Targeting P4HA3.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2255)
  AUTHORS   Zhou,H., Zou,J., Shao,C., Zhou,A., Yu,J., Chen,S. and Xu,C.
  TITLE     Prolyl 4-hydroxylase subunit alpha 3 facilitates human colon cancer
            growth and metastasis through the TGF-beta/Smad signaling pathway
  JOURNAL   Pathol Res Pract 230, 153749 (2022)
   PUBMED   34959098
  REMARK    GeneRIF: Prolyl 4-hydroxylase subunit alpha 3 facilitates human
            colon cancer growth and metastasis through the TGF-beta/Smad
            signaling pathway.
REFERENCE   4  (bases 1 to 2255)
  AUTHORS   Li,M., Wang,Q., Zheng,Q., Wu,L., Zhao,B. and Wu,Y.
  TITLE     Prognostic and diagnostic roles of prolyl 4-hydroxylase subunit
            alpha members in breast cancer
  JOURNAL   Biomark Med 15 (13), 1085-1095 (2021)
   PUBMED   34387118
  REMARK    GeneRIF: Prognostic and diagnostic roles of prolyl 4-hydroxylase
            subunit alpha members in breast cancer.
REFERENCE   5  (bases 1 to 2255)
  AUTHORS   Gu,X., Wang,B., Zhu,H., Zhou,Y., Horning,A.M., Huang,T.H., Chen,Y.,
            Houghton,P., Lai,Z., Michalek,J.E. and Sun,L.Z.
  TITLE     Age-associated genes in human mammary gland drive human breast
            cancer progression
  JOURNAL   Breast Cancer Res 22 (1), 64 (2020)
   PUBMED   32539762
  REMARK    GeneRIF: Age-associated genes in human mammary gland drive human
            breast cancer progression.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2255)
  AUTHORS   Song,H., Liu,L., Song,Z., Ren,Y., Li,C. and Huo,J.
  TITLE     P4HA3 is Epigenetically Activated by Slug in Gastric Cancer and its
            Deregulation is Associated With Enhanced Metastasis and Poor
            Survival
  JOURNAL   Technol Cancer Res Treat 17, 1533033818796485 (2018)
   PUBMED   30198421
  REMARK    GeneRIF: Based on findings above, we infer that P4HA3 is
            epigenetically activated by Slug, and its deregulation is
            associated with enhanced metastasis and poor survival of gastric
            cancer.
REFERENCE   7  (bases 1 to 2255)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   8  (bases 1 to 2255)
  AUTHORS   Koivunen,P., Hirsila,M., Kivirikko,K.I. and Myllyharju,J.
  TITLE     The length of peptide substrates has a marked effect on
            hydroxylation by the hypoxia-inducible factor prolyl 4-hydroxylases
  JOURNAL   J Biol Chem 281 (39), 28712-28720 (2006)
   PUBMED   16885164
  REMARK    GeneRIF: HIF-P4H, HIF-1alpha and HIF-2alpha are effective oxygen
            sensors
REFERENCE   9  (bases 1 to 2255)
  AUTHORS   Kukkola,L., Hieta,R., Kivirikko,K.I. and Myllyharju,J.
  TITLE     Identification and characterization of a third human, rat, and
            mouse collagen prolyl 4-hydroxylase isoenzyme
  JOURNAL   J Biol Chem 278 (48), 47685-47693 (2003)
   PUBMED   14500733
  REMARK    GeneRIF: analysis of collagen prolyl 4-hydroxylase isoenzyme 3, its
            expression and catalytic properties
REFERENCE   10 (bases 1 to 2255)
  AUTHORS   Van Den Diepstraten,C., Papay,K., Bolender,Z., Brown,A. and
            Pickering,J.G.
  TITLE     Cloning of a novel prolyl 4-hydroxylase subunit expressed in the
            fibrous cap of human atherosclerotic plaque
  JOURNAL   Circulation 108 (5), 508-511 (2003)
   PUBMED   12874193
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY004548.1 and AY358521.1.
            
            On Nov 22, 2018 this sequence version replaced NM_182904.4.
            
            Summary: This gene encodes a component of prolyl 4-hydroxylase, a
            key enzyme in collagen synthesis composed of two identical alpha
            subunits and two beta subunits. The encoded protein is one of
            several different types of alpha subunits and provides the major
            part of the catalytic site of the active enzyme. In collagen and
            related proteins, prolyl 4-hydroxylase catalyzes the formation of
            4-hydroxyproline that is essential to the proper three-dimensional
            folding of newly synthesized procollagen chains. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Jan 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the shorter isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358521.1, BC089446.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000331597.9/ ENSP00000332170.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 HY004548.1         38-46
            10-2255             AY358521.1         1-2246
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.4"
     gene            1..2255
                     /gene="P4HA3"
                     /note="prolyl 4-hydroxylase subunit alpha 3"
                     /db_xref="GeneID:283208"
                     /db_xref="HGNC:HGNC:30135"
                     /db_xref="MIM:608987"
     exon            1..229
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..1664
                     /gene="P4HA3"
                     /EC_number="1.14.11.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; collagen prolyl 4-hydroxylase alpha(III);
                     procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline
                     4-hydroxylase), alpha polypeptide III; C-P4H alpha III;
                     4-PH alpha-3; prolyl 4-hydroxylase, alpha polypeptide III"
                     /codon_start=1
                     /product="prolyl 4-hydroxylase subunit alpha-3 isoform 1
                     precursor"
                     /protein_id="NP_878907.1"
                     /db_xref="CCDS:CCDS8230.1"
                     /db_xref="GeneID:283208"
                     /db_xref="HGNC:HGNC:30135"
                     /db_xref="MIM:608987"
                     /translation="MGPGARLAALLAVLALGTGDPERAAARGDTFSALTSVARALAPE
                     RRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRN
                     VVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQ
                     RVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEW
                     KTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLYSPDNKRMARNVLKYERLLAE
                     SPNHVVAEAVIQRPNIPHLQTRDTYEGLCQTLGSQPTLYQIPSLYCSYETNSNAYLLL
                     QPIRKEVIHLEPYIALYHDFVSDSEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKS
                     AWLKDTVDPKLVTLNHRIAALTGLDVRPPYAEYLQVVNYGIGGHYEPHFDHATSPSSP
                     LYRMKSGNRVATFMIYLSSVEAGGATAFIYANLSVPVVRNAALFWWNLHRSGEGDSDT
                     LHAGCPVLVGDKWVANKWIHEYGQEFRRPCSSSPED"
     exon            230..372
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     exon            373..596
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     exon            597..746
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     exon            747..798
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     exon            799..962
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1139
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1204
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1364
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1427
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1496
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1593
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1594..2255
                     /gene="P4HA3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2235..2240
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P4HA3"
                     /note="hexamer: ATTAAA"
     polyA_site      2255
                     /gene="P4HA3"
                     /note="major polyA site"
ORIGIN      
        1 gtctgagggg aagttcgcga gcgctggcta tgggtcctgg ggcgcggctg gcggcgctgc
       61 tggcggtgct ggcgctcggg acaggagacc cagaaagggc tgcggctcgg ggcgacacgt
      121 tctcggcgct gaccagcgtg gcgcgcgccc tggcgcccga gcgccggctg ctggggctgc
      181 tgaggcggta cctgcgcggg gaggaggcgc ggctgcggga cctgactaga ttctacgaca
      241 aggtactttc tttgcatgag gattcaacaa cccctgtggc taaccctctg cttgcattta
      301 ctctcatcaa acgcctgcag tctgactgga ggaatgtggt acatagtctg gaggccagtg
      361 agaacatccg agctctgaag gatggctatg agaaggtgga gcaagacctt ccagcctttg
      421 aggaccttga gggagcagca agggccctga tgcggctgca ggacgtgtac atgctcaatg
      481 tgaaaggcct ggcccgaggt gtctttcaga gagtcactgg ctctgccatc actgacctgt
      541 acagccccaa acggctcttt tctctcacag gggatgactg cttccaagtt ggcaaggtgg
      601 cctatgacat gggggattat taccatgcca ttccatggct ggaggaggct gtcagtctct
      661 tccgaggatc ttacggagag tggaagacag aggatgaggc aagtctagaa gatgccttgg
      721 atcacttggc ctttgcttat ttccgggcag gaaatgtttc gtgtgccctc agcctctctc
      781 gggagtttct tctctacagc ccagataata agaggatggc caggaatgtc ttgaaatatg
      841 aaaggctctt ggcagagagc cccaaccacg tggtagctga ggctgtcatc cagaggccca
      901 atatacccca cctgcagacc agagacacct acgaggggct atgtcagacc ctgggttccc
      961 agcccactct ctaccagatc cctagcctct actgttccta tgagaccaat tccaacgcct
     1021 acctgctgct ccagcccatc cggaaggagg tcatccacct ggagccctac attgctctct
     1081 accatgactt cgtcagtgac tcagaggctc agaaaattag agaacttgca gaaccatggc
     1141 tacagaggtc agtggtggca tcaggggaga agcagttaca agtggagtac cgcatcagca
     1201 aaagtgcctg gctgaaggac actgttgacc caaaactggt gaccctcaac caccgcattg
     1261 ctgccctcac aggccttgat gtccggcctc cctatgcaga gtatctgcag gtggtgaact
     1321 atggcatcgg aggacactat gagcctcact ttgaccatgc tacgtcacca agcagccccc
     1381 tctacagaat gaagtcagga aaccgagttg caacatttat gatctatctg agctcggtgg
     1441 aagctggagg agccacagcc ttcatctatg ccaacctcag cgtgcctgtg gttaggaatg
     1501 cagcactgtt ttggtggaac ctgcacagga gtggtgaagg ggacagtgac acacttcatg
     1561 ctggctgtcc tgtcctggtg ggagataagt gggtggccaa caagtggata catgagtatg
     1621 gacaggaatt ccgcagaccc tgcagctcca gccctgaaga ctgaactgtt ggcagagaga
     1681 agctggtgga gtcctgtggc tttccagaga agccaggagc caaaagctgg ggtaggagag
     1741 gagaaagcag agcagcctcc tggaagaagg ccttgtcagc tttgtctgtg cctcgcaaat
     1801 cagaggcaag ggagaggttg ttaccagggg acactgagaa tgtacatttg atctgcccca
     1861 gccacggaag tcagagtagg atgcacagta caaaggaggg gggagtggag gcctgagagg
     1921 gaagtttctg gagttcagat actctctgtt gggaacagga catctcaaca gtctcaggtt
     1981 cgatcagtgg gtcttttggc actttgaacc ttgaccacag ggaccaagaa gtggcaatga
     2041 ggacacctgc aggaggggct agcctgactc ccagaacttt aagactttct ccccactgcc
     2101 ttctgctgca gcccaagcag ggagtgtccc cctcccagaa gcatatccca gatgagtggt
     2161 acattatata aggatttttt ttaagttgaa aacaactttc ttttcttttt gtatgatggt
     2221 tttttaacac agtcattaaa aatgtttata aatca
//
