LOCUS       NP_002556                365 aa            linear   PRI 30-APR-2025
DEFINITION  P2Y purinoceptor 4 [Homo sapiens].
ACCESSION   NP_002556
VERSION     NP_002556.1
DBSOURCE    REFSEQ: accession NM_002565.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 365)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (residues 1 to 365)
  AUTHORS   Sukigara,S., Dai,H., Nabatame,S., Otsuki,T., Hanai,S., Honda,R.,
            Saito,T., Nakagawa,E., Kaido,T., Sato,N., Kaneko,Y., Takahashi,A.,
            Sugai,K., Saito,Y., Sasaki,M., Goto,Y., Koizumi,S. and Itoh,M.
  TITLE     Expression of astrocyte-related receptors in cortical dysplasia
            with intractable epilepsy
  JOURNAL   J Neuropathol Exp Neurol 73 (8), 798-806 (2014)
   PUBMED   25003238
  REMARK    GeneRIF: reflected in the immunoblotting data P2RY4 receptors were
            detected at higher levels of expression in patient with cortical
            dysplasia with intractable epilepsy.
REFERENCE   3  (residues 1 to 365)
  AUTHORS   Tran,M.D., Furones-Alonso,O., Sanchez-Molano,J. and Bramlett,H.M.
  TITLE     Trauma-induced expression of astrocytic thrombospondin-1 is
            regulated by P2 receptors coupled to protein kinase cascades
  JOURNAL   Neuroreport 23 (12), 721-726 (2012)
   PUBMED   22776902
  REMARK    GeneRIF: show that P2 receptors and protein kinases are also
            involved in regulating injury-induced expression and the release of
            TSP-1 in injured astrocytes
REFERENCE   4  (residues 1 to 365)
  AUTHORS   Borno,A., Ploug,T., Bune,L.T., Rosenmeier,J.B. and Thaning,P.
  TITLE     Purinergic receptors expressed in human skeletal muscle fibres
  JOURNAL   Purinergic Signal 8 (2), 255-264 (2012)
   PUBMED   22052557
  REMARK    GeneRIF: P2Y4 receptors are expressed in intracellular vesicles and
            sarcolemma of skeletal muscle fibers.
REFERENCE   5  (residues 1 to 365)
  AUTHORS   Luo,W., Wang,Y. and Reiser,G.
  TITLE     Proteinase-activated receptors, nucleotide P2Y receptors, and
            mu-opioid receptor-1B are under the control of the type I
            transmembrane proteins p23 and p24A in post-Golgi trafficking
  JOURNAL   J Neurochem 117 (1), 71-81 (2011)
   PUBMED   21219331
  REMARK    GeneRIF: Nucleotide P2Y4 receptor binding is arrested in post-Golgi
            trafficking by type I transmembrane proteins p23 and p24A.
REFERENCE   6  (residues 1 to 365)
  AUTHORS   Maier,R., Glatz,A., Mosbacher,J. and Bilbe,G.
  TITLE     Cloning of P2Y6 cDNAs and identification of a pseudogene:
            comparison of P2Y receptor subtype expression in bone and brain
            tissues
  JOURNAL   Biochem Biophys Res Commun 237 (2), 297-302 (1997)
   PUBMED   9268704
REFERENCE   7  (residues 1 to 365)
  AUTHORS   Nicholas,R.A., Watt,W.C., Lazarowski,E.R., Li,Q. and Harden,K.
  TITLE     Uridine nucleotide selectivity of three phospholipase C-activating
            P2 receptors: identification of a UDP-selective, a UTP-selective,
            and an ATP- and UTP-specific receptor
  JOURNAL   Mol Pharmacol 50 (2), 224-229 (1996)
   PUBMED   8700127
REFERENCE   8  (residues 1 to 365)
  AUTHORS   Stam,N.J., Klomp,J., Van de Heuvel,N. and Olijve,W.
  TITLE     Molecular cloning and characterization of a novel orphan receptor
            (P2P) expressed in human pancreas that shows high structural
            homology to the P2U purinoceptor
  JOURNAL   FEBS Lett 384 (3), 260-264 (1996)
   PUBMED   8617367
REFERENCE   9  (residues 1 to 365)
  AUTHORS   Communi,D., Pirotton,S., Parmentier,M. and Boeynaems,J.M.
  TITLE     Cloning and functional expression of a human uridine nucleotide
            receptor
  JOURNAL   J Biol Chem 270 (52), 30849-30852 (1995)
   PUBMED   8537336
REFERENCE   10 (residues 1 to 365)
  AUTHORS   Nguyen,T., Erb,L., Weisman,G.A., Marchese,A., Heng,H.H.,
            Garrad,R.C., George,S.R., Turner,J.T. and O'Dowd,B.F.
  TITLE     Cloning, expression, and chromosomal localization of the human
            uridine nucleotide receptor gene
  JOURNAL   J Biol Chem 270 (52), 30845-30848 (1995)
   PUBMED   8537335
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL357752.19.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: The product of this gene belongs to the family of
            G-protein coupled receptors. This family has several receptor
            subtypes with different pharmacological selectivity, which overlaps
            in some cases, for various adenosine and uridine nucleotides. This
            receptor is responsive to uridine nucleotides, partially responsive
            to ATP, and not responsive to ADP. [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: BC095503.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374519.4/ ENSP00000363643.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13.1"
     Protein         1..365
                     /product="P2Y purinoceptor 4"
                     /note="uridine nucleotide receptor; pyrimidinergic
                     receptor P2Y, G-protein coupled, 4"
                     /calculated_mol_wt=40832
     Site            35..61
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P51582.1)"
     Region          36..317
                     /region_name="7tmA_P2Y4"
                     /note="P2Y purinoceptor 4, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd15374"
                     /db_xref="CDD:320496"
     Region          36..63
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320496"
     Region          70..95
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320496"
     Site            73..95
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P51582.1)"
     Site            order(91,94..95,108..113,115..116,119,164,166..170,184,
                     186..188,197,200..202,204..206,208..209,258,261..262,
                     264..265,268,284..285,287..289,292,295..296)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:320496"
     Region          108..138
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320496"
     Site            113..131
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P51582.1)"
     Region          150..172
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320496"
     Site            155..174
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P51582.1)"
     Region          197..226
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320496"
     Site            197..222
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P51582.1)"
     Region          238..268
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320496"
     Site            247..269
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P51582.1)"
     Region          285..310
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320496"
     Site            288..309
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P51582.1)"
     Site            333
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000305|PubMed:11114308; propagated from
                     UniProtKB/Swiss-Prot (P51582.1)"
     Site            334
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000305|PubMed:11114308; propagated from
                     UniProtKB/Swiss-Prot (P51582.1)"
     CDS             1..365
                     /gene="P2RY4"
                     /gene_synonym="NRU; P2P; P2Y4; UNR"
                     /coded_by="NM_002565.4:581..1678"
                     /db_xref="CCDS:CCDS14398.1"
                     /db_xref="GeneID:5030"
                     /db_xref="HGNC:HGNC:8542"
                     /db_xref="MIM:300038"
ORIGIN      
        1 mastessllr slglspgpgs seveldcwfd edfkfillpv syavvfvlgl glnaptlwlf
       61 ifrlrpwdat atymfhlals dtlyvlslpt liyyyaahnh wpfgteickf vrflfywnly
      121 csvlfltcis vhrylgichp lralrwgrpr lagllclavw lvvagclvpn lffvttsnkg
      181 ttvlchdttr peefdhyvhf ssavmgllfg vpclvtlvcy glmarrlyqp lpgsaqsssr
      241 lrslrtiavv ltvfavcfvp fhitrtiyyl arlleadcrv lnivnvvykv trplasansc
      301 ldpvlylltg dkyrrqlrql cgggkpqprt aasslalvsl pedsscrwaa tpqdsscstp
      361 radrl
//
