LOCUS       NM_022788               2244 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens purinergic receptor P2Y12 (P2RY12), transcript variant
            1, mRNA.
ACCESSION   NM_022788
VERSION     NM_022788.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Nolfi-Donegan,D., Annarapu,G.K., St Croix,C., Calderon,M.,
            Hillery,C.A. and Shiva,S.
  TITLE     High-mobility group box 1 increases platelet surface P2Y12 and
            platelet activation in sickle cell disease
  JOURNAL   JCI Insight 9 (5), e174575 (2024)
   PUBMED   38456510
  REMARK    GeneRIF: High-mobility group box 1 increases platelet surface P2Y12
            and platelet activation in sickle cell disease.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Amoafo,E.B., Entsie,P., Kang,Y., Canobbio,I. and Liverani,E.
  TITLE     Platelet P2Y12 signalling pathway in the dysregulated immune
            response during sepsis
  JOURNAL   Br J Pharmacol 181 (4), 532-546 (2024)
   PUBMED   37525937
  REMARK    GeneRIF: Platelet P2Y12 signalling pathway in the dysregulated
            immune response during sepsis.
            Review article
REFERENCE   3  (bases 1 to 2244)
  AUTHORS   Brambilla,M., Becchetti,A., Rovati,G.E., Cosentino,N., Conti,M.,
            Canzano,P., Giesen,P.L.A., Loffreda,A., Bonomi,A., Cattaneo,M., De
            Candia,E., Podda,G.M., Trabattoni,D., Werba,P.J., Campodonico,J.,
            Pinna,C., Marenzi,G., Tremoli,E. and Camera,M.
  TITLE     Cell Surface Platelet Tissue Factor Expression: Regulation by P2Y12
            and Link to Residual Platelet Reactivity
  JOURNAL   Arterioscler Thromb Vasc Biol 43 (10), 2042-2057 (2023)
   PUBMED   37589138
  REMARK    GeneRIF: Cell Surface Platelet Tissue Factor Expression: Regulation
            by P2Y12 and Link to Residual Platelet Reactivity.
REFERENCE   4  (bases 1 to 2244)
  AUTHORS   Hidayat,R., Rasyid,A., Harris,S., Harahap,A., Herqutanto,
            Louisa,M., Listiyaningsih,E., Rambe,A.S. and Loho,T.
  TITLE     Correlation P2Y12 Genetic Polymorphism As Risk Factor of
            Clopidogrel Resistance in Indonesian Stroke Patients
  JOURNAL   Vasc Health Risk Manag 19, 53-61 (2023)
   PUBMED   36743859
  REMARK    GeneRIF: Correlation P2Y12 Genetic Polymorphism As Risk Factor of
            Clopidogrel Resistance in Indonesian Stroke Patients.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2244)
  AUTHORS   Su,C., Zhang,Z., Chen,J., Tian,M., Wu,C. and Zhang,T.
  TITLE     Association between P2Y1 and P2Y12 polymorphisms and acute
            myocardial infarction and ADP-induced platelet aggregation
  JOURNAL   BMC Cardiovasc Disord 23 (1), 41 (2023)
   PUBMED   36681816
  REMARK    GeneRIF: Association between P2Y1 and P2Y12 polymorphisms and acute
            myocardial infarction and ADP-induced platelet aggregation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2244)
  AUTHORS   Takasaki,J., Kamohara,M., Saito,T., Matsumoto,M., Matsumoto,S.,
            Ohishi,T., Soga,T., Matsushime,H. and Furuichi,K.
  TITLE     Molecular cloning of the platelet P2T(AC) ADP receptor:
            pharmacological comparison with another ADP receptor, the P2Y(1)
            receptor
  JOURNAL   Mol Pharmacol 60 (3), 432-439 (2001)
   PUBMED   11502873
REFERENCE   7  (bases 1 to 2244)
  AUTHORS   Nicholas,R.A.
  TITLE     Identification of the P2Y(12) receptor: a novel member of the P2Y
            family of receptors activated by extracellular nucleotides
  JOURNAL   Mol Pharmacol 60 (3), 416-420 (2001)
   PUBMED   11502870
  REMARK    Review article
REFERENCE   8  (bases 1 to 2244)
  AUTHORS   Zhang,F.L., Luo,L., Gustafson,E., Lachowicz,J., Smith,M., Qiao,X.,
            Liu,Y.H., Chen,G., Pramanik,B., Laz,T.M., Palmer,K., Bayne,M. and
            Monsma,F.J. Jr.
  TITLE     ADP is the cognate ligand for the orphan G protein-coupled receptor
            SP1999
  JOURNAL   J Biol Chem 276 (11), 8608-8615 (2001)
   PUBMED   11104774
REFERENCE   9  (bases 1 to 2244)
  AUTHORS   Hollopeter,G., Jantzen,H.M., Vincent,D., Li,G., England,L.,
            Ramakrishnan,V., Yang,R.B., Nurden,P., Nurden,A., Julius,D. and
            Conley,P.B.
  TITLE     Identification of the platelet ADP receptor targeted by
            antithrombotic drugs
  JOURNAL   Nature 409 (6817), 202-207 (2001)
   PUBMED   11196645
REFERENCE   10 (bases 1 to 2244)
  AUTHORS   Ohlmann,P., Laugwitz,K.L., Nurnberg,B., Spicher,K., Schultz,G.,
            Cazenave,J.P. and Gachet,C.
  TITLE     The human platelet ADP receptor activates Gi2 proteins
  JOURNAL   Biochem J 312 (Pt 3) (Pt 3), 775-779 (1995)
   PUBMED   8554519
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC313627.1, AF313449.1,
            AK292096.1, AW976204.1, DB306976.1, AW015793.1 and AA810452.1.
            
            On Dec 4, 2019 this sequence version replaced NM_022788.4.
            
            Summary: The product of this gene belongs to the family of
            G-protein coupled receptors. This family has several receptor
            subtypes with different pharmacological selectivity, which overlaps
            in some cases, for various adenosine and uridine nucleotides. This
            receptor is involved in platelet aggregation, and is a potential
            target for the treatment of thromboembolisms and other clotting
            disorders. Mutations in this gene are implicated in bleeding
            disorder, platelet type 8 (BDPLT8). Alternative splicing results in
            multiple transcript variants of this gene. [provided by RefSeq, Jul
            2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.67090.1,
                                           SRR18074968.1173253.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000302632.4/ ENSP00000307259.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-494               DC313627.1         60-553
            495-1499            AF313449.1         328-1332
            1500-1707           AK292096.1         1498-1705
            1708-2038           AW976204.1         225-555
            2039-2111           DB306976.1         137-209             c
            2112-2239           AW015793.1         15-142              c
            2240-2244           AA810452.1         30-34               c
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q25.1"
     gene            1..2244
                     /gene="P2RY12"
                     /gene_synonym="ADPG-R; BDPLT8; HORK3; P2T(AC); P2Y(12)R;
                     P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999"
                     /note="purinergic receptor P2Y12"
                     /db_xref="GeneID:64805"
                     /db_xref="HGNC:HGNC:18124"
                     /db_xref="MIM:600515"
     exon            1..62
                     /gene="P2RY12"
                     /gene_synonym="ADPG-R; BDPLT8; HORK3; P2T(AC); P2Y(12)R;
                     P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999"
                     /inference="alignment:Splign:2.1.0"
     exon            63..227
                     /gene="P2RY12"
                     /gene_synonym="ADPG-R; BDPLT8; HORK3; P2T(AC); P2Y(12)R;
                     P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999"
                     /inference="alignment:Splign:2.1.0"
     exon            228..2244
                     /gene="P2RY12"
                     /gene_synonym="ADPG-R; BDPLT8; HORK3; P2T(AC); P2Y(12)R;
                     P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999"
                     /inference="alignment:Splign:2.1.0"
     CDS             242..1270
                     /gene="P2RY12"
                     /gene_synonym="ADPG-R; BDPLT8; HORK3; P2T(AC); P2Y(12)R;
                     P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999"
                     /note="G-protein coupled receptor SP1999; Gi-coupled ADP
                     receptor HORK3; putative G-protein coupled receptor;
                     ADP-glucose receptor; purinergic receptor P2RY12;
                     purinergic receptor P2Y, G-protein coupled, 12; P2Y12
                     platelet ADP receptor"
                     /codon_start=1
                     /product="P2Y purinoceptor 12"
                     /protein_id="NP_073625.1"
                     /db_xref="CCDS:CCDS3159.1"
                     /db_xref="GeneID:64805"
                     /db_xref="HGNC:HGNC:18124"
                     /db_xref="MIM:600515"
                     /translation="MQAVDNLTSAPGNTSLCTRDYKITQVLFPLLYTVLFFVGLITNG
                     LAMRIFFQIRSKSNFIIFLKNTVISDLLMILTFPFKILSDAKLGTGPLRTFVCQVTSV
                     IFYFTMYISISFLGLITIDRYQKTTRPFKTSNPKNLLGAKILSVVIWAFMFLLSLPNM
                     ILTNRQPRDKNVKKCSFLKSEFGLVWHEIVNYICQVIFWINFLIVIVCYTLITKELYR
                     SYVRTRGVGKVPRKKVNVKVFIIIAVFFICFVPFHFARIPYTLSQTRDVFDCTAENTL
                     FYVKESTLWLTSLNACLDPFIYFFLCKSFRNSLISMLKCPNSATSLSQDNRKKEQDGG
                     DPNEETPM"
     regulatory      2227..2232
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P2RY12"
                     /gene_synonym="ADPG-R; BDPLT8; HORK3; P2T(AC); P2Y(12)R;
                     P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999"
                     /note="hexamer: ATTAAA"
     polyA_site      2244
                     /gene="P2RY12"
                     /gene_synonym="ADPG-R; BDPLT8; HORK3; P2T(AC); P2Y(12)R;
                     P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999"
                     /note="major polyA site"
ORIGIN      
        1 acaatcagaa gacaggagct gcagaacaga acactttctc atgtccaggg tcagattaca
       61 agagcactca agactttact gacgaaaact caggaaatcc tctatcacaa agaggtttgg
      121 caactaaact aagacattaa aaggaaaata ccagatgcca ctctgcaggt tgcaataact
      181 actacttact ggatacattc aaaccctcca gaatcaacag ttatcaggta accaacaaga
      241 aatgcaagcc gtcgacaacc tcacctctgc gcctggtaac accagtctgt gcaccagaga
      301 ctacaaaatc acccaggtcc tcttcccact gctctacact gtcctgtttt ttgttggact
      361 tatcacaaat ggcctggcga tgaggatttt ctttcaaatc cggagtaaat caaactttat
      421 tatttttctt aagaacacag tcatttctga tcttctcatg attctgactt ttccattcaa
      481 aattcttagt gatgccaaac tgggaacagg accactgaga acttttgtgt gtcaagttac
      541 ctccgtcata ttttatttca caatgtatat cagtatttca ttcctgggac tgataactat
      601 cgatcgctac cagaagacca ccaggccatt taaaacatcc aaccccaaaa atctcttggg
      661 ggctaagatt ctctctgttg tcatctgggc attcatgttc ttactctctt tgcctaacat
      721 gattctgacc aacaggcagc cgagagacaa gaatgtgaag aaatgctctt tccttaaatc
      781 agagttcggt ctagtctggc atgaaatagt aaattacatc tgtcaagtca ttttctggat
      841 taatttctta attgttattg tatgttatac actcattaca aaagaactgt accggtcata
      901 cgtaagaacg aggggtgtag gtaaagtccc caggaaaaag gtgaacgtca aagttttcat
      961 tatcattgct gtattcttta tttgttttgt tcctttccat tttgcccgaa ttccttacac
     1021 cctgagccaa acccgggatg tctttgactg cactgctgaa aatactctgt tctatgtgaa
     1081 agagagcact ctgtggttaa cttccttaaa tgcatgcctg gatccgttca tctatttttt
     1141 cctttgcaag tccttcagaa attccttgat aagtatgctg aagtgcccca attctgcaac
     1201 atctctgtcc caggacaata ggaaaaaaga acaggatggt ggtgacccaa atgaagagac
     1261 tccaatgtaa acaaattaac taaggaaata tttcaatctc tttgtgttca gaactcgtta
     1321 aagcaaagcg ctaagtaaaa atattaactg acgaagaagc aactaagtta ataataatga
     1381 ctctaaagaa acagaagatt acaaaagcaa ttttcattta cctttccagt atgaaaagct
     1441 atcttaaaat atagaaaact aatctaaact gtagctgtat tagcagcaaa acaaacgaca
     1501 tccaattgtc atgctgcatg caaaactaca cagaattcat gttttgcaga gttttgccaa
     1561 aatgagtaat catataatat ttactgtaat ttttaaaata cattatcgtt cacaatttta
     1621 ttttttcata atcaactaag gaagaacgat caattggata taatcttctt accaaaaatg
     1681 atagttaaaa tgtatatata tcctagtccc ctaaccaaat cctgacctat tgggatactt
     1741 ataaaaattt aagtaagtgg gatacacaaa gaataataac tattaacttt tcattattag
     1801 caaaaaccta agggatttaa actaattgaa actgtatttg attggactta attttttatg
     1861 tttatttaga agataaagat ttaaagaaga cctttacaat aaagagaaga aatatcgaag
     1921 tcattaaaat aaggagactt acttttatga cattctaata ctaaaaaata tagaaatatt
     1981 tccttaattc tagagaaact agttttacta attttttaca acttcaataa taccatcatt
     2041 gacacttacc tttattaatt agcttctaga aaatagctgc taattaggtt aatgaacatt
     2101 ttaccttagt gaaaaaaatt aattaaatat gattacaaag ttgcacagca taactactga
     2161 gaggaaagtg attgatctgt ttgtaattac ttgtttgtat tggtgtgtat aaaatacaaa
     2221 atttacatta aactctaaat catt
//
