LOCUS       NM_002559               3792 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens purinergic receptor P2X 3 (P2RX3), mRNA.
ACCESSION   NM_002559 XM_937250
VERSION     NM_002559.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Li,M., Wang,Y., Banerjee,R., Marinelli,F., Silberberg,S.,
            Faraldo-Gomez,J.D., Hattori,M. and Swartz,K.J.
  TITLE     Molecular mechanisms of human P2X3 receptor channel activation and
            modulation by divalent cation bound ATP
  JOURNAL   Elife 8, e47060 (2019)
   PUBMED   31232692
  REMARK    GeneRIF: The authors used structural, computational and functional
            approaches to show that a crucial acidic chamber near the
            nucleotide-binding pocket in human P2X3 receptors accommodates
            divalent ions in two distinct modes in the absence and presence of
            nucleotide.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Wang,J., Wang,Y., Cui,W.W., Huang,Y., Yang,Y., Liu,Y., Zhao,W.S.,
            Cheng,X.Y., Sun,W.S., Cao,P., Zhu,M.X., Wang,R., Hattori,M. and
            Yu,Y.
  TITLE     Druggable negative allosteric site of P2X3 receptors
  JOURNAL   Proc Natl Acad Sci U S A 115 (19), 4939-4944 (2018)
   PUBMED   29674445
  REMARK    GeneRIF: Druggable negative allosteric site of P2X3 receptors.
REFERENCE   3  (bases 1 to 3792)
  AUTHORS   Burnstock,G.
  TITLE     The therapeutic potential of purinergic signalling
  JOURNAL   Biochem Pharmacol 151, 157-165 (2018)
   PUBMED   28735873
  REMARK    Review article
REFERENCE   4  (bases 1 to 3792)
  AUTHORS   Ding,S., Zhu,L., Tian,Y., Zhu,T., Huang,X. and Zhang,X.
  TITLE     P2X3 receptor involvement in endometriosis pain via ERK signaling
            pathway
  JOURNAL   PLoS One 12 (9), e0184647 (2017)
   PUBMED   28898282
  REMARK    GeneRIF: P2X3 expressions in endometriosis endometrium and
            endometriotic lesions were significantly higher as compared with
            control endometrium, and positively correlated with pain.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3792)
  AUTHORS   Kawate,T.
  TITLE     P2X Receptor Activation
  JOURNAL   Adv Exp Med Biol 1051, 55-69 (2017)
   PUBMED   28639248
  REMARK    Review article
REFERENCE   6  (bases 1 to 3792)
  AUTHORS   North,R.A.
  TITLE     P2X receptors
  JOURNAL   Philos Trans R Soc Lond B Biol Sci 371 (1700) (2016)
   PUBMED   27377721
  REMARK    Review article
REFERENCE   7  (bases 1 to 3792)
  AUTHORS   Bianchi,B.R., Lynch,K.J., Touma,E., Niforatos,W., Burgard,E.C.,
            Alexander,K.M., Park,H.S., Yu,H., Metzger,R., Kowaluk,E.,
            Jarvis,M.F. and van Biesen,T.
  TITLE     Pharmacological characterization of recombinant human and rat P2X
            receptor subtypes
  JOURNAL   Eur J Pharmacol 376 (1-2), 127-138 (1999)
   PUBMED   10440098
REFERENCE   8  (bases 1 to 3792)
  AUTHORS   Garcia-Guzman,M., Stuhmer,W. and Soto,F.
  TITLE     Molecular characterization and pharmacological properties of the
            human P2X3 purinoceptor
  JOURNAL   Brain Res Mol Brain Res 47 (1-2), 59-66 (1997)
   PUBMED   9221902
REFERENCE   9  (bases 1 to 3792)
  AUTHORS   Lewis,C., Neidhart,S., Holy,C., North,R.A., Buell,G. and
            Surprenant,A.
  TITLE     Coexpression of P2X2 and P2X3 receptor subunits can account for
            ATP-gated currents in sensory neurons
  JOURNAL   Nature 377 (6548), 432-435 (1995)
   PUBMED   7566120
REFERENCE   10 (bases 1 to 3792)
  AUTHORS   Kennedy,C. and Leff,P.
  TITLE     Painful connection for ATP
  JOURNAL   Nature 377 (6548), 385-386 (1995)
   PUBMED   7566110
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY032450.1, DB462670.1,
            BC074792.2, BC069162.1 and AP000781.6.
            
            On May 17, 2019 this sequence version replaced NM_002559.4.
            
            Summary: This gene encodes a member of the P2X purinergic receptor
            (purinoceptor) gene family which includes seven members (P2RX1 -
            P2RX7). P2X purinoceptors are a family of cation-permeable,
            ligand-gated ion channels that open in response to the binding of
            extracellular adenosine 5'-triphosphate (ATP). The encoded protein
            is a subunit of the trimeric P2X3 receptor ion channel which is
            expressed by sensory or autonomic neurons. A deficiency of the
            orthologous protein in mice is associated with reduced pain-related
            behavior and urinary bladder hyporeflexia. [provided by RefSeq, Aug
            2017].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC074792.2, AB016608.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263314.3/ ENSP00000263314.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                HY032450.1         4-18
            16-168              DB462670.1         1-153
            169-1432            BC074792.2         1-1264
            1433-1472           BC069162.1         1307-1346
            1473-3792           AP000781.6         125393-127712
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.1"
     gene            1..3792
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /note="purinergic receptor P2X 3"
                     /db_xref="GeneID:5024"
                     /db_xref="HGNC:HGNC:8534"
                     /db_xref="MIM:600843"
     exon            1..318
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..1393
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /note="ATP receptor; P2X receptor, subunit 3; purinoceptor
                     P2X3; purinergic receptor P2X3; purinergic receptor P2X,
                     ligand gated ion channel, 3"
                     /codon_start=1
                     /product="P2X purinoceptor 3"
                     /protein_id="NP_002550.2"
                     /db_xref="CCDS:CCDS7953.1"
                     /db_xref="GeneID:5024"
                     /db_xref="HGNC:HGNC:8534"
                     /db_xref="MIM:600843"
                     /translation="MNCISDFFTYETTKSVVVKSWTIGIINRVVQLLIISYFVGWVFL
                     HEKAYQVRDTAIESSVVTKVKGSGLYANRVMDVSDYVTPPQGTSVFVIITKMIVTENQ
                     MQGFCPESEEKYRCVSDSQCGPERLPGGGILTGRCVNYSSVLRTCEIQGWCPTEVDTV
                     ETPIMMEAENFTIFIKNSIRFPLFNFEKGNLLPNLTARDMKTCRFHPDKDPFCPILRV
                     GDVVKFAGQDFAKLARTGGVLGIKIGWVCDLDKAWDQCIPKYSFTRLDSVSEKSSVSP
                     GYNFRFAKYYKMENGSEYRTLLKAFGIRFDVLVYGNAGKFNIIPTIISSVAAFTSVGV
                     GTVLCDIILLNFLKGADQYKAKKFEEVNETTLKIAALTNPVYPSDQTTAEKQSTDSGA
                     FSIGH"
     exon            319..454
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     exon            455..526
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     exon            527..590
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     exon            591..684
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     exon            685..762
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     exon            763..904
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1041
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1135
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1201
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1279
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..3792
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3452..3457
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /note="hexamer: GATAAA"
     polyA_site      3461
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /note="major polyA site"
     polyA_site      3472
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
     regulatory      3776..3781
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
                     /note="hexamer: AAGAAA"
     polyA_site      3792
                     /gene="P2RX3"
                     /gene_synonym="P2X3"
ORIGIN      
        1 ggcctcttgg aagccagagt atcaagagca gagaatctca ctaggattgc atggcttaaa
       61 gggacaggct ccccattcct ccaacccctc taagctgccc cctccaggtc gtgatctcgt
      121 ctccctgtcc tgtaggacct ccctctcctg aggccaccac tgggccccct tctgagtgtc
      181 ccctgagcac tctctcagca tgaactgcat atccgacttc ttcacctatg agaccaccaa
      241 gtcggtggtt gtgaagagct ggaccatcgg gatcatcaac cgagtagttc agcttctgat
      301 catctcctac tttgtagggt gggttttctt gcacgagaag gcttaccagg tacgggacac
      361 agccattgag tcctcggtgg taaccaaggt gaagggctcc ggactctacg ccaacagagt
      421 catggatgtg tctgattacg tgacgccacc tcagggcacc tcggtctttg tcatcatcac
      481 caagatgatt gttactgaaa atcagatgca aggattctgc ccagagagtg aggagaaata
      541 ccgctgtgta tcagacagcc agtgcgggcc tgagcgcttg ccaggtgggg ggatcctcac
      601 tggccgctgc gtgaactaca gctctgtgct ccggacctgt gagatccagg gctggtgccc
      661 cacggaggtg gacacagtgg aaacgcccat catgatggaa gctgagaact tcactatttt
      721 catcaagaac agcatccgtt tccccctctt caactttgag aagggaaacc tccttcccaa
      781 cctgacagcc agggacatga agacctgccg cttccacccg gacaaggacc ctttctgccc
      841 catcttgcgg gtaggggacg tggtcaagtt tgcggggcag gattttgcca aactggcgcg
      901 cacgggggga gttctgggca ttaagatcgg ctgggtgtgc gacttggaca aggcctggga
      961 ccagtgcatc cccaaatact ccttcacccg gctcgacagc gtttctgaga aaagcagcgt
     1021 gtccccaggc tacaacttca ggtttgccaa gtactacaaa atggaaaatg gcagtgagta
     1081 ccgcaccctc ctgaaggctt ttggcatccg cttcgacgtg ctggtatacg ggaatgctgg
     1141 caagttcaac atcatcccca ccatcatcag ctctgtggcg gcctttactt ctgtgggagt
     1201 gggaactgtt ctctgtgaca tcatcctgct caacttcctc aagggggccg accagtacaa
     1261 agccaagaag tttgaggagg tgaatgagac tacgctgaaa atcgcggctt tgaccaaccc
     1321 agtgtacccc agcgaccaga ccacagcgga gaagcagtcc accgattcgg gggccttctc
     1381 cataggccac tagggcctct ttccagggcc ccacactcac aaaggctcca ggcctcccca
     1441 cagaggaccc tgcctgagca aggggcatgg gagggaagag gggctctcat ttctgctgct
     1501 cattccatga gcatagctgg gacccaagtg tctgggcctc cgactgctcc agcagacagg
     1561 cagtgctccc tgctgagacc ccaatctcac cttcactcct tgcctggccc catctgcttc
     1621 ctaggacccc tggggcagga gcacctgagc catccccttc ccaaagagta gagattataa
     1681 tgtaggacag atggccacaa gggcctacca agtgccaggc actttcacac acgttatctc
     1741 atttaatcct tagaataatc ctatgaggta gatattagtt tcccttgttt tgaagataaa
     1801 ccaaggctca gagagactga gtcatttgcc ccaggccaga tagccaggat gtgagagagc
     1861 tgggatttga acgtccgtct gactaactcc atcgcccaca ccccatgaga gaagaatgaa
     1921 ctcccagggt ccatcagccc tgctgcttca gccgcctcca ccctgacggt gattcggtta
     1981 ataaagagta agccccatgc ctttcccagc aacaaagttt gcattttagg gagactggtt
     2041 agacagtggt gagggacccc atctctgact tgggtgcatc ccaaatttag aaggaaacag
     2101 aaggcatggc tgtcagtgaa tttttcctca tcactcccca tagctctctc cccaaaccag
     2161 ggaaggggtc attcttgttc ttctctggga ccccacaccc acacacatct gggccccctc
     2221 actctgggga tttgattccc ggacctggga tcctgtcgta ggctgggacc ctgttccagg
     2281 agttcagcag tcctcattta caacaacagt gaaaatgctc cagtaaggcc tggtgccttg
     2341 tgagctgctc agctgggttg gccaggcctg tccccatagg gtcttaataa ggtgccccat
     2401 tttctaaggt tagaagctaa aggggccttt taaaccatct tatctaaccc tcttgcttac
     2461 agatgagcaa actgaggcca gaaagggaaa atgactggtt cagtgccaca gttcatggcc
     2521 aaaaaggaac ccacatgtcc tggcccttca gggaaggagg caagaacagg aagctggatc
     2581 cattgaccca gacatcctct atcccctttc tgtcctctcc tacagtgaga ggcccagaaa
     2641 ttgccccagg gcagggctct ggggcagaac caggttttgt gaatcctaag catatacaat
     2701 ttgggggcat ctctttagaa agaatacaaa attaggtatt agaaggggct catgcaagtg
     2761 aatgagccta aagaatgttt caatagcttc atgataaatc tgcatctggc agtcagagtg
     2821 ggcatgatca tgaggtctta gctgcaccag ctcaactgta gatggagggc ggcccctcaa
     2881 cacacggcac aggaagtggg gaagggacag cttcaggagt agttcaggag gctcaaatgg
     2941 tgggtacttg caaaggcctt gcccaatgac cccccgctcc agggggagaa ggggtaagac
     3001 ccaccccaca taaagctcaa caccccttcc ctcactctgc cctaacccac agccccatgc
     3061 ccaggatggt ggggcatatc tccccagggt cagccttggg aattgggcag tgcctggaga
     3121 tctgaccaga ctatgcttct ccagcagaga catcagtgaa ggcagcacca cattctccac
     3181 ctgggactcc cccaggcatc cctggggaat tcagctcttg ggaggctggg gtcacaggga
     3241 ttctctctaa tggggagatg gaggcagtgg tgtcctgaag ggaccctccc ccccaacccc
     3301 tcccttttac ccagatggag gtggtcctcc tgtaggtgcc tccgcccctc tggagccggc
     3361 cagcaagtgt gtgtgagcct gaggttgtcc tccgcatatc tgaactaagt taccacttct
     3421 gtgtgctaga tgtagggtta atatcagtag tgataaaaac agctaccttg tctctgttgc
     3481 attatggttt actcagcaca atcaccaaca taatcacatg gaccctcaca ccaacgctgg
     3541 tgagtccgct atggtttgtc attaatccta tttgatagaa aaagaaattt acagtacgtg
     3601 gaaatgagat ctggaaccta tgcccattcc taacactaca ttgcctcttt aatcattcaa
     3661 cataaaagcc aacactgatt acttactatt ggcaggcaat ggaccaaatg agtgacaggg
     3721 attgtctaac tcaaccccca ctttacagaa gaagaaacag atgcatacta aaataaagaa
     3781 actgaaatag aa
//
