LOCUS       NM_138348               7969 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens OTU deubiquitinase with linear linkage specificity
            (OTULIN), mRNA.
ACCESSION   NM_138348
VERSION     NM_138348.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Quoc,Q.L., Kim,Y., Park,G., Cao,T.B.T., Choi,Y., Park,Y.H. and
            Park,H.S.
  TITLE     Downregulation of otulin induces inflammasome activation in
            neutrophilic asthma
  JOURNAL   J Allergy Clin Immunol 154 (3), 557-570 (2024)
   PUBMED   38599290
  REMARK    GeneRIF: Downregulation of otulin induces inflammasome activation
            in neutrophilic asthma.
REFERENCE   2  (bases 1 to 7969)
  AUTHORS   Batlle-Maso,L., Antolin,M., Marques-Soares,J., Dieli-Crimi,R. and
            Colobran,R.
  TITLE     OTULIN Haploinsufficiency Causes Hyperinflammatory Responses to
            Infectious and Non-Infectious Triggers
  JOURNAL   J Clin Immunol 44 (4), 95 (2024)
   PUBMED   38578307
  REMARK    GeneRIF: OTULIN Haploinsufficiency Causes Hyperinflammatory
            Responses to Infectious and Non-Infectious Triggers.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7969)
  AUTHORS   Huang,Z., Zhang,Z., Tu,Y., He,H., Qiu,F., Qian,H. and Pan,C.
  TITLE     Integrated Single-Cell and Transcriptome Sequencing Analyses
            Develop a Ubiquitination-Associated Signature in Gastric Cancer and
            Identified OTULIN as a Novel Biomarker
  JOURNAL   Front Biosci (Landmark Ed) 28 (11), 304 (2023)
   PUBMED   38062820
  REMARK    GeneRIF: Integrated Single-Cell and Transcriptome Sequencing
            Analyses Develop a Ubiquitination-Associated Signature in Gastric
            Cancer and Identified OTULIN as a Novel Biomarker.
REFERENCE   4  (bases 1 to 7969)
  AUTHORS   Almeida,S.M., Ivantsiv,S., Niibori,R., Dunham,W.H., Green,B.A.,
            Zhao,L., Gingras,A.C. and Cordes,S.P.
  TITLE     An interaction between OTULIN and SCRIB uncovers roles for linear
            ubiquitination in planar cell polarity
  JOURNAL   Dis Model Mech 16 (8) (2023)
   PUBMED   37589075
  REMARK    GeneRIF: An interaction between OTULIN and SCRIB uncovers roles for
            linear ubiquitination in planar cell polarity.
REFERENCE   5  (bases 1 to 7969)
  AUTHORS   Li,M., Li,L., Asemota,S., Kakhniashvili,D., Narayanan,R., Wang,X.
            and Liao,F.F.
  TITLE     Reciprocal interplay between OTULIN-LUBAC determines genotoxic and
            inflammatory NF-kappaB signal responses
  JOURNAL   Proc Natl Acad Sci U S A 119 (33), e2123097119 (2022)
   PUBMED   35939695
  REMARK    GeneRIF: Reciprocal interplay between OTULIN-LUBAC determines
            genotoxic and inflammatory NF-kappaB signal responses.
REFERENCE   6  (bases 1 to 7969)
  AUTHORS   Takiuchi,T., Nakagawa,T., Tamiya,H., Fujita,H., Sasaki,Y.,
            Saeki,Y., Takeda,H., Sawasaki,T., Buchberger,A., Kimura,T. and
            Iwai,K.
  TITLE     Suppression of LUBAC-mediated linear ubiquitination by a specific
            interaction between LUBAC and the deubiquitinases CYLD and OTULIN
  JOURNAL   Genes Cells 19 (3), 254-272 (2014)
   PUBMED   24461064
  REMARK    GeneRIF: cylindromatosis (CYLD) and OTULIN/Gumby/Fam105B, directly
            interact with the N-terminal PUB domain-containing region of HOIP.
REFERENCE   7  (bases 1 to 7969)
  AUTHORS   Mevissen,T.E., Hospenthal,M.K., Geurink,P.P., Elliott,P.R.,
            Akutsu,M., Arnaudo,N., Ekkebus,R., Kulathu,Y., Wauer,T., El
            Oualid,F., Freund,S.M., Ovaa,H. and Komander,D.
  TITLE     OTU deubiquitinases reveal mechanisms of linkage specificity and
            enable ubiquitin chain restriction analysis
  JOURNAL   Cell 154 (1), 169-184 (2013)
   PUBMED   23827681
REFERENCE   8  (bases 1 to 7969)
  AUTHORS   Fiil,B.K., Damgaard,R.B., Wagner,S.A., Keusekotten,K., Fritsch,M.,
            Bekker-Jensen,S., Mailand,N., Choudhary,C., Komander,D. and
            Gyrd-Hansen,M.
  TITLE     OTULIN restricts Met1-linked ubiquitination to control innate
            immune signaling
  JOURNAL   Mol Cell 50 (6), 818-830 (2013)
   PUBMED   23806334
  REMARK    GeneRIF: OTULIN restricts Met1-ubiquitin formation after immune
            receptor stimulation to prevent unwarranted proinflammatory
            signaling.
REFERENCE   9  (bases 1 to 7969)
  AUTHORS   Rivkin,E., Almeida,S.M., Ceccarelli,D.F., Juang,Y.C., MacLean,T.A.,
            Srikumar,T., Huang,H., Dunham,W.H., Fukumura,R., Xie,G., Gondo,Y.,
            Raught,B., Gingras,A.C., Sicheri,F. and Cordes,S.P.
  TITLE     The linear ubiquitin-specific deubiquitinase gumby regulates
            angiogenesis
  JOURNAL   Nature 498 (7454), 318-324 (2013)
   PUBMED   23708998
  REMARK    GeneRIF: 2.4 A and 2.8 A crystal structures of gumby (Fam105b) in
            the presence of free ubiquitin and linear diubiquitin substrate,
            respectively
REFERENCE   10 (bases 1 to 7969)
  AUTHORS   Keusekotten,K., Elliott,P.R., Glockner,L., Fiil,B.K.,
            Damgaard,R.B., Kulathu,Y., Wauer,T., Hospenthal,M.K.,
            Gyrd-Hansen,M., Krappmann,D., Hofmann,K. and Komander,D.
  TITLE     OTULIN antagonizes LUBAC signaling by specifically hydrolyzing
            Met1-linked polyubiquitin
  JOURNAL   Cell 153 (6), 1312-1326 (2013)
   PUBMED   23746843
  REMARK    GeneRIF: reveal a previously unannotated human DUB, OTULIN (also
            known as FAM105B), which is exquisitely specific for Met1 linkages;
            data suggest that OTULIN regulates Met1-polyUb signaling.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010491.4, BC015392.1,
            CK817982.1, BC007706.2 and DB238080.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_138348.5.
            
            Summary: This gene encodes a member of the peptidase C65 family of
            ubiquitin isopeptidases. Members of this family remove ubiquitin
            from proteins. The encoded enzyme specifically recognizes and
            removes M1(Met1)-linked, or linear, ubiquitin chains from protein
            substrates. Linear ubiquitin chains are known to regulate the
            NF-kappa B signaling pathway in the context of immunity and
            inflammation. Mutations in this gene cause a potentially fatal
            autoinflammatory syndrome in human patients. [provided by RefSeq,
            Sep 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.178863.1,
                                           SRR7346977.903172.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000284274.5/ ENSP00000284274.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AC010491.4         57392-57421
            31-1489             BC015392.1         1-1459
            1490-2064           CK817982.1         16-590
            2065-2963           BC007706.2         1682-2580
            2964-7393           AC010491.4         87519-91948
            7394-7969           DB238080.1         1-576
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p15.2"
     gene            1..7969
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /note="OTU deubiquitinase with linear linkage specificity"
                     /db_xref="GeneID:90268"
                     /db_xref="HGNC:HGNC:25118"
                     /db_xref="MIM:615712"
     exon            1..260
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..1167
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /EC_number="3.4.19.12"
                     /note="ubiquitin thioesterase Gumby; deubiquitinating
                     enzyme otulin; OTU domain-containing deubiquitinase with
                     linear linkage specificity; family with sequence
                     similarity 105, member B"
                     /codon_start=1
                     /product="ubiquitin thioesterase otulin"
                     /protein_id="NP_612357.4"
                     /db_xref="CCDS:CCDS43302.1"
                     /db_xref="GeneID:90268"
                     /db_xref="HGNC:HGNC:25118"
                     /db_xref="MIM:615712"
                     /translation="MSRGTMPQPEAWPGASCAETPAREAAATARDGGKAAASGQPRPE
                     MQCPAEHEEDMYRAADEIEKEKELLIHERGASEPRLSVAPEMDIMDYCKKEWRGNTQK
                     ATCMKMGYEEVSQKFTSIRRVRGDNYCALRATLFQAMSQAVGLPPWLQDPELMLLPEK
                     LISKYNWIKQWKLGLKFDGKNEDLVDKIKESLTLLRKKWAGLAEMRTAEARQIACDEL
                     FTNEAEEYSLYEAVKFLMLNRAIELYNDKEKGKEVPFFSVLLFARDTSNDPGQLLRNH
                     LNQVGHTGGLEQVEMFLLAYAVRHTIQVYRLSKYNTEEFITVYPTDPPKDWPVVTLIA
                     EDDRHYNIPVRVCEETSL"
     exon            261..337
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /inference="alignment:Splign:2.1.0"
     exon            338..432
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /inference="alignment:Splign:2.1.0"
     exon            433..576
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /inference="alignment:Splign:2.1.0"
     exon            577..702
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /inference="alignment:Splign:2.1.0"
     exon            703..972
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /inference="alignment:Splign:2.1.0"
     exon            973..7969
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6702..6707
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /note="hexamer: AATAAA"
     polyA_site      6725
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /note="major polyA site"
     regulatory      7812..7817
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
                     /note="hexamer: AATAAA"
     polyA_site      7853
                     /gene="OTULIN"
                     /gene_synonym="AIPDS; AIPDSA; FAM105B; GUM; IMD107"
ORIGIN      
        1 ggaaaccgcc ccggcggctg agaggctgcg gccactgcct ggcaccccga cgggaggggc
       61 tccggatcgt tcggagccgg ctgaacccct tcggccgcga gcgaccgcat gagtcggggg
      121 actatgcccc agcccgaagc gtggccaggc gcgagctgcg ccgagacgcc ggcgcgggag
      181 gcggcggcca cggcgcggga cggcgggaag gcggcggcca gcgggcagcc gcggcccgag
      241 atgcagtgcc cggccgagca tgaggaggac atgtaccgtg ctgcagatga aatagaaaag
      301 gagaaagaat tgcttataca tgaaagaggg gcatcagaac cgagattaag cgtagctcct
      361 gaaatggata tcatggacta ctgcaaaaaa gaatggagag gaaatacaca gaaagcaacg
      421 tgtatgaaaa tgggctatga agaggtttct cagaagttca cctccatacg gcgagtccgt
      481 ggtgataatt actgtgcact gagggccacg ctgttccagg ccatgagcca ggctgtgggg
      541 ctgccgccct ggctgcagga cccggagctc atgctgttac cagaaaaact cataagcaaa
      601 tacaactgga tcaagcaatg gaaacttgga ctgaaatttg atgggaagaa tgaggacctg
      661 gttgataaaa ttaaagagtc ccttactctg ctgaggaaga agtgggcagg cttggctgaa
      721 atgagaactg ctgaagcaag acagatagct tgtgatgaac tattcacaaa tgaggcggag
      781 gaatatagcc tctatgaagc tgtaaaattt ctaatgctaa acagagccat tgaactatat
      841 aatgataaag agaaaggaaa ggaagtacca tttttctctg tgcttctgtt tgctcgggac
      901 acatcaaatg acccaggaca gcttctgagg aaccacctca accaggtggg acacactggt
      961 ggtcttgaac aggttgaaat gttccttctt gcctatgctg tgcgccacac catccaggtg
     1021 taccggctct ccaagtacaa cacggaagaa ttcatcacag tctaccccac cgacccaccc
     1081 aaggactggc cagtggtaac gctcattgct gaggacgatc ggcactataa catccccgtc
     1141 agagtgtgtg aggagaccag tctatgagag acgcatgctc ctgacagcct ggcgacgtgg
     1201 cgaagatgca caggtggctc ctgggcttgg gctgcaggtt tgggggtctc taagaacaat
     1261 ctctgagaag aacccttggg cccctgggag ccaagttgga caggatgtcc tgaagactag
     1321 cttttgataa gagaaattaa ccaagtcttt cccctcatct atgatgcaat atatttcagt
     1381 gggggccttc agagcacacc tgttggacgg tgcaaaccat atcttctcca gaaggcaaat
     1441 acttttgtat cagaggaaac tcagttttgg agaggaatat gttctttatg tctcaaatca
     1501 aaactctctc taatggtaaa ctggcttcta atttttttaa gtacagtatt tttttttccc
     1561 ctttagtagt aacgggtttc tatagatctt cctatacagt ctgctttaac tcaggacctt
     1621 gagattatga gactgacgtg ctgcccactg cactgagggg gcttctaaca gtctgcttta
     1681 agtggtataa ttctgggata gatctgttac tggcatagtc atgacaacct ctggtaatct
     1741 taccttctcc tttttatgaa gggaagagca atggtttgga cttacatctt aattaaggct
     1801 attttaagca gattgttttg caacagatta agaattgggt cccaaagtgg gttatttcaa
     1861 ggcatttttg aagactggag gagcgtagga gggagtggtg agggaaccta gaacttcttg
     1921 ctccttgtga ctatgacaga tgtctgatgc ccccagcgca ctggagtctg gggcttggtg
     1981 caggtggtgc cccatcagtg tggacagaca ctacttgcct ttgtggttta gtgttggaag
     2041 ctttaattat tctacagttc catagattca caattttata gccaaacagt ttgtatccac
     2101 ctgctttcag aggaggaacc aaaggccaaa ttactttgag gtagggctta gctggacctg
     2161 ggttttccta tgctcttttc atgctgtgtt ggagggtgtg tccactgcca gatctgtgta
     2221 acccgtctgg gtcaggggat gagtgacagc aaaccatctt aaaatttttc atgagtacat
     2281 ttaagcggaa gcatacggga atatgagtgc aaaagtgtgg ctgagccgcg tatgcccttg
     2341 attggttttg ggaagcctga gggaggcagc cttcctggct atgagccatc gcctgcccaa
     2401 tcaggctaag ggtggtgact ggggtggtga aggggcagct ctgctgagca tggtctgcct
     2461 tatggcctga attgtcctca aggggtgtgg actgcagatg gtgttcacat gaaccggaga
     2521 catcactctt taggattcta ctggcagccc ctgaattggc tcaacgtttg tggaggtggt
     2581 atttccctga agtactgagc tttgttttat aataattaaa atccttattt ggtccaattt
     2641 aatatagttt agaagctatt ttttttgagg caaaccgttt ttgaaaatgt aaattttgtt
     2701 tttaattaaa aataaagtct tagttaagaa aactcaggat gcacaaaact attcagactg
     2761 ttactttagc tttctcccat tacctcatca gtaatattca ccacattttg ctaaatattt
     2821 attgattaga gtgttgaaat caaattctgc tctaaatgtg tgtgaatatg ttggagaggc
     2881 tttgtgttct tcactgtgaa atgcaattgt gccttgaata agaaggtacc tagaagccaa
     2941 attaaagtaa taatgacttc ttattggctt tgatttttca ttgcagtata tgggattgta
     3001 cagcaggaaa tgcttatcat taatttctga tgttttttaa agcacaactc gaaacatttc
     3061 gatcatacat acatagcagt agagatctgt gcccttcagg tacattgaat ctgaccatca
     3121 gtttatatat gtcattgaat tttaagaata ctcatgttaa taatagtcat ctatccttgc
     3181 attttgaaac tgttctaatc ttagtgaact tgaattggat ttctgggtaa aagaatgtgt
     3241 ttcttttatg ttgcttatgt ccgaaggcct tgtcagaatc tgtcagactc ttgtttaggt
     3301 ttagtgtgat catggcgtca gagaagcaaa gctttcaaat aaatagtact tcaggaaata
     3361 gaaatgattg accaacttta aaaataattt ttttttaatt gcaatatgca gcttcagttg
     3421 cccagaatct tagttccgtt tctcattctt ggtcttgagc tggtcaggtg acatcagcag
     3481 attagaagtt gaatggagat taagtggatt caggaggatg ttccacttag agcagtcttc
     3541 aaaatgataa ggtgttctag aagaaaggaa tgtagtagga actatactat gcctaacttt
     3601 ctatcccaga gtgtcttgca agagtttagg agttttggac cctgtgtatt ggcagaaaag
     3661 ttatctccat cttaagcagg catgactttt atacctgtga gctcatttaa ggtgcattta
     3721 aacctaaaat aatttccctg tattatgctt catgggatta acactgcttt tccagaacat
     3781 tttcagattc ccctccttac atcctgagct ccttctgtat atacatctgt tgattttatc
     3841 catccacaag gaacaatgat agtcacatta gagaacaaga aaccagtaat acatggtctc
     3901 taactgatga ttcgggcctg gatttgattg aaagtgtttg cagttcctct tccgtagaat
     3961 acagagtgga tgaaaatgtt ttcaatgcac agaacaggat gaatcctttt ttctttattt
     4021 agcgatttac acttttgtta ctctattata tattcagtta gtgtctgata agattttctt
     4081 tgcttaagga gaacggacat tgccttggta tgtttttttt tttttttccc tccacttttg
     4141 gagcttatca ggtaaaaatc tcaagccaca tgaattgtta acacctctgt tgggaaaagc
     4201 ctttgtgagt ttttatgtac ttggtctttg tttttgttat tcatcctgtg tcctccctct
     4261 tcccgatgtg ctgttttacc taggagttag tctgctttct gaggatcttt tagagagagg
     4321 ctgtgaagtg ctgaatcacc tttaatgata cagcacttct gccatctcag catctacata
     4381 ggacttacat agacttcctg aatgtgtctt cttcagatac taaagtacag ttggatcatt
     4441 ttcttatctc cttttcttaa gcagtacttt gcaggtactc ccctttgaaa gccagaagca
     4501 taaaccattg gggaatctta acttgtagac atgcagtaaa agaaatgcat ttatgtaaga
     4561 tctgtgagta cttaaaaaga aagccctcag tgtgtgtgaa gtgaatgtga aatgtgtgtg
     4621 aaatacatag aattcccaaa tagtttagca aaggcagggc gcaatatcaa gtaatttaaa
     4681 aatggtccaa ggaactgtaa gaaggaggaa ctaattctag aataaatgtt aaaatgccat
     4741 tcaagaacaa aaccacagat gccatacaga cctcctgtgc ttaagttata gaagaataaa
     4801 aatctgaatg aatggaaggc cttacgtgta tacagtttac aaattcctat ttctaaaatt
     4861 taagtccctt atttaacaga agtatgtatt ttaatgctta actgtctcgg gaaacctcat
     4921 ttgtgacatc atctaagggg atgggaagac tagggagcca gtgccacgtt gaacagaaca
     4981 gtggtttagt gaatgtgtga ggaaagacat gggcaactga ttattaatgt ttttgtaatt
     5041 cagtttataa cttggaacca atgaaaagca acaaaactaa actggtttga cagcctgcca
     5101 cttctggcat ttcctgtaag tcactagcag taggtgtgag gtgggcttgc ccatgaccag
     5161 gaggggtgtg tgtgtgtgtg tgcatgtgtg tatatgcgtg ttggtctgca gtcacagcat
     5221 acctttatgt gcatgtgtcc tcgcagcttg ggactcagca gtattctggg agggtggagg
     5281 tgaactgtcc catgtattgt attatatatt ttttgagatg gggtcttgct ctgttgccca
     5341 ggctggagtg cagtggtgcg atctcagctc actgcaactt ttgcctcctg gttcaagcag
     5401 ttctcctgcc tcagcctgcc aaatagctgg gattacaggt gtgtaccacc actcccagct
     5461 aatttttgta tttttagtag agatggggtt ttaccatgtt ggccaggctg gtctcgagct
     5521 cctggcctca ggtgatccac ctgctttggc ctcccaaagt gctgagatta caggcgtgaa
     5581 ctaccgcgcc tggccccatg tattgtattt ttttcaggtt atattgaaat ctactaccag
     5641 gaatgtcgga atgggttttg gtatgtataa tggaaataga tagagtggtt aagtctagaa
     5701 acacatacat taattgtatt gaaatgttat atcaatacat catttatgat gtgtgtgtgg
     5761 tcccagacct catggccacc agtttgttta agcattgtga atgcttttta atagcattca
     5821 ttagcattaa tggaggagga cactgtgttt tctcaattaa tctcattgat ttgtttggta
     5881 taagtttggg tcagaaatga aactgccaaa acatcgatca gtacaaggaa gggacacagg
     5941 gcttaaaatg tccacagtct tggcagtgga cttggcagtt ctcccagtaa gcagaagtac
     6001 ttgagcttaa ttctgaactt caaagtaata ttttatactt aattttagga gttttcattt
     6061 acatattgaa aaatgccttg actgtattca cataaatggt gctaaaacat tgtacccctt
     6121 ataagaactg cagcaatcca cagtaatgtt ggttacttct gagtatttga taaaggaaca
     6181 aagtcaaaat gaatgtattt aataagcttc tttctcattt ccattgtttt tataaaaata
     6241 ttttggtatt gttgcctgca ttttagccac ttctaacttt ttgtattatg aatttggaga
     6301 ggataacaag ccaactttag accctctgcc agtggcgatg gtgtttttct tccatgggta
     6361 agccattgtg aatggaggct ggtggagcag cacactgtgt gaacctggca gcgctcatct
     6421 tggcttgttt agcagtgcct ctgtttacac cataagatgt tccgcatgtg tccaaaattt
     6481 cgcctgctct gaaaaacatg cccccgaggc tctgtgcagt ttggggcctt ggttcccacc
     6541 tgcagatgcg gtcagttgcc tggctcctgg ggccagagtt tcctctgtta cttgttgagt
     6601 ctcttgtctt cactgtcaga agctgaactg acttgggggc tttgctgttg atccacttta
     6661 gcaaaccctg ctgcagagga ctgtaaaaac aaataactaa aaataaactt agaaaataca
     6721 actcagaagc ctggctctgt tgctgtggtc aaatgcttgc ctttgaggac tttggcagat
     6781 tgtgctatta cattgggctc taactttctg gcacccgcaa ggcagaccta tgatgtccac
     6841 atggctgact cttgacccct ggccattgtg agcagaggaa tcaagggttt agggaggcag
     6901 tattgggcag tgtggaagag ttagatgaga tgaagcagtt agcacagtgc ttaacccata
     6961 ctaagtgccc ccaaccctgc cagccaaatt aggtgcatag ttttcagctt gggtctggat
     7021 tcaacagcat tcccgctgcc cactaaaagg gtgggcttgg acagttcctg aacctcccta
     7081 aatctgtttc ttcatcttta ccacacacag tctgtctcag tggtggtgtg gattaagtga
     7141 ggtgctagca cttattaggg gaagatcctc cagacctgca aagcagggct gcagccccaa
     7201 ttctgaccca gccctgctgt gtgtttgccg caaagaggtt tggtgtgtgc gagacatttc
     7261 tttatgaacc atttacactg ctggaaatgt acccactaat gtgatggcaa gaacttcaac
     7321 aaattgctta tgaatcaggc tctcaatgga agaagttaat cctctgaaga gggggaagac
     7381 tggactttca gaagtatgtt ggggatagag aatgaagttt gagtcagcag agctctgcct
     7441 tcctgtttgt caaagaacta gagtctggga gtcttgcaag atggagctgg gcttctgaag
     7501 aacccagtgg gtaggtactg ggcatccatt agacggcagt gctaagtcgg tcatgtgcag
     7561 cagaaagacc tccccactgt gaacaacaaa ccactttcct cctccagtgg cccattcatt
     7621 tggattgata cactttttca gtgtcagaaa tgcactttct ccctctactt gtctgaatta
     7681 cgatgctctg ctcaactctg tggacagtgt ttcttgaatt tccttttcca ccgccttttc
     7741 ccactcaaga aagtggagag aaaaacaggg atgcaggctg tgggtcttgt ggaagccctt
     7801 tacaaccatt aaataaagaa caccagctgc attatgtgtg tttagaacga gaagttgttt
     7861 gtacagtatt tttctattga ccgcttccgt cttgcctgaa acctgggcat tctttctgta
     7921 gtttctctga attttctgtc tcacctcctc acccctaccc ttgcctttt
//
