LOCUS       NM_023112               3911 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens OTU deubiquitinase, ubiquitin aldehyde binding 2
            (OTUB2), mRNA.
ACCESSION   NM_023112
VERSION     NM_023112.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Zhu,W., Wu,C., Liu,Z., Zhao,S. and Huang,J.
  TITLE     OTU deubiquitinase, ubiquitin aldehyde binding 2 (OTUB2) modulates
            the stemness feature, chemoresistance, and epithelial-mesenchymal
            transition of colon cancer via regulating GINS complex subunit 1
            (GINS1) expression
  JOURNAL   Cell Commun Signal 22 (1), 420 (2024)
   PUBMED   39210373
  REMARK    GeneRIF: OTU deubiquitinase, ubiquitin aldehyde binding 2 (OTUB2)
            modulates the stemness feature, chemoresistance, and
            epithelial-mesenchymal transition of colon cancer via regulating
            GINS complex subunit 1 (GINS1) expression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Guo,C., Zhang,M., Jin,X., Zhu,C., Qian,J. and Tao,M.
  TITLE     Exploring the regulatory role of FBXL19-AS1 in triple-negative
            breast cancer through the miR-378a-3p/OTUB2 axis
  JOURNAL   Cell Biochem Funct 42 (4), e4020 (2024)
   PUBMED   38702967
  REMARK    GeneRIF: Exploring the regulatory role of FBXL19-AS1 in
            triple-negative breast cancer through the miR-378a-3p/OTUB2 axis.
REFERENCE   3  (bases 1 to 3911)
  AUTHORS   Beck,A., Vinik,Y., Shatz-Azoulay,H., Isaac,R., Streim,S., Jona,G.,
            Boura-Halfon,S. and Zick,Y.
  TITLE     Otubain 2 is a novel promoter of beta cell survival as revealed by
            siRNA high-throughput screens of human pancreatic islets
  JOURNAL   Diabetologia 56 (6), 1317-1326 (2013)
   PUBMED   23515685
  REMARK    GeneRIF: OTUB2 is a novel promoter of viability and insulin
            secretion in human beta cells. OTUB2 acts through the inhibition of
            NF-kappaB signalling.
REFERENCE   4  (bases 1 to 3911)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   5  (bases 1 to 3911)
  AUTHORS   Li,S., Zheng,H., Mao,A.P., Zhong,B., Li,Y., Liu,Y., Gao,Y., Ran,Y.,
            Tien,P. and Shu,H.B.
  TITLE     Regulation of virus-triggered signaling by OTUB1- and
            OTUB2-mediated deubiquitination of TRAF3 and TRAF6
  JOURNAL   J Biol Chem 285 (7), 4291-4297 (2010)
   PUBMED   19996094
  REMARK    GeneRIF: Findings suggest that OTUB1 and OTUB2 negatively regulate
            virus-triggered type I IFN induction and cellular antiviral
            response by deubiquitinating TRAF3 and -6.
REFERENCE   6  (bases 1 to 3911)
  AUTHORS   Sowa,M.E., Bennett,E.J., Gygi,S.P. and Harper,J.W.
  TITLE     Defining the human deubiquitinating enzyme interaction landscape
  JOURNAL   Cell 138 (2), 389-403 (2009)
   PUBMED   19615732
REFERENCE   7  (bases 1 to 3911)
  AUTHORS   Edelmann,M.J., Iphofer,A., Akutsu,M., Altun,M., di Gleria,K.,
            Kramer,H.B., Fiebiger,E., Dhe-Paganon,S. and Kessler,B.M.
  TITLE     Structural basis and specificity of human otubain 1-mediated
            deubiquitination
  JOURNAL   Biochem J 418 (2), 379-390 (2009)
   PUBMED   18954305
REFERENCE   8  (bases 1 to 3911)
  AUTHORS   Nanao,M.H., Tcherniuk,S.O., Chroboczek,J., Dideberg,O., Dessen,A.
            and Balakirev,M.Y.
  TITLE     Crystal structure of human otubain 2
  JOURNAL   EMBO Rep 5 (8), 783-788 (2004)
   PUBMED   15258613
  REMARK    GeneRIF: Data report the first crystal structure of an OTU
            superfamily protein, otubain 2, at 2.1 A resolution and propose a
            model for otubain-ubiquitin binding.
REFERENCE   9  (bases 1 to 3911)
  AUTHORS   Namciu,S.J., Friedman,R.D., Marsden,M.D., Sarausad,L.M.,
            Jasoni,C.L. and Fournier,R.E.
  TITLE     Sequence organization and matrix attachment regions of the human
            serine protease inhibitor gene cluster at 14q32.1
  JOURNAL   Mamm Genome 15 (3), 162-178 (2004)
   PUBMED   15014966
REFERENCE   10 (bases 1 to 3911)
  AUTHORS   Balakirev,M.Y., Tcherniuk,S.O., Jaquinod,M. and Chroboczek,J.
  TITLE     Otubains: a new family of cysteine proteases in the ubiquitin
            pathway
  JOURNAL   EMBO Rep 4 (5), 517-522 (2003)
   PUBMED   12704427
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL079302.7.
            
            On Nov 23, 2018 this sequence version replaced NM_023112.3.
            
            Summary: This gene encodes one of several deubiquitylating enzymes.
            Ubiquitin modification of proteins is needed for their stability
            and function; to reverse the process, deubiquityling enzymes remove
            ubiquitin. This protein contains an OTU domain and binds Ubal
            (ubiquitin aldehyde); an active cysteine protease site is present
            in the OTU domain. [provided by RefSeq, Aug 2011].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK025569.1, SRR1803612.175176.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000203664.10/ ENSP00000203664.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               AL079302.7         111677-111877
            202-297             AL079302.7         122717-122812
            298-416             AL079302.7         124300-124418
            417-501             AL079302.7         129308-129392
            502-696             AL079302.7         129923-130117
            697-3911            AL079302.7         131053-134267
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.12"
     gene            1..3911
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /note="OTU deubiquitinase, ubiquitin aldehyde binding 2"
                     /db_xref="GeneID:78990"
                     /db_xref="HGNC:HGNC:20351"
                     /db_xref="MIM:608338"
     exon            1..201
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     CDS             199..903
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /EC_number="3.4.19.12"
                     /note="ubiquitin-specific protease otubain 2; otubain-2;
                     deubiquitinating enzyme OTUB2;
                     ubiquitin-specific-processing protease OTUB2; OTU
                     domain-containing ubiquitin aldehyde-binding protein 2;
                     OTU domain, ubiquitin aldehyde binding 2"
                     /codon_start=1
                     /product="ubiquitin thioesterase OTUB2"
                     /protein_id="NP_075601.1"
                     /db_xref="CCDS:CCDS9917.1"
                     /db_xref="GeneID:78990"
                     /db_xref="HGNC:HGNC:20351"
                     /db_xref="MIM:608338"
                     /translation="MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRK
                     TKGDGNCFYRALGYSYLESLLGKSREIFKFKERVLQTPNDLLAAGFEEHKFRNFFNAF
                     YSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDI
                     KDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYL
                     LYKTSHYNILYAADKH"
     exon            202..297
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     exon            298..416
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     exon            417..501
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     exon            502..696
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     exon            697..3911
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      892..897
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /note="hexamer: GATAAA"
     polyA_site      912
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /note="major polyA site"
     polyA_site      961
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /note="major polyA site"
     regulatory      3893..3898
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /note="hexamer: ATTAAA"
     polyA_site      3911
                     /gene="OTUB2"
                     /gene_synonym="C14orf137; OTB2; OTU2"
                     /note="major polyA site"
ORIGIN      
        1 agggcgtgtg tcttgctggg acacagtgga ggtctaacct ttggtttgcg gagcggtcgg
       61 gtgtattctc cgccgccccc acgccctcga ggtccccgcc accgaaccag cggcggagcc
      121 cgcccgcgcc tcccgcggca ttcccgcacc ggatcgctcc tcgctggggc gggacctggc
      181 ctggcggctc tggtcactat gagtgaaaca tctttcaacc taatatcaga aaaatgtgac
      241 attctatcca ttcttcggga ccatcctgaa aacaggattt accggaggaa aatcgaggaa
      301 ctcagcaaaa ggttcaccgc catccgcaag accaaagggg atgggaactg cttctacagg
      361 gccttgggct attcctacct ggagtccctg ctggggaaga gcagggagat cttcaagttc
      421 aaagaacgcg tactgcagac cccaaatgac cttctggctg ctggctttga ggagcacaag
      481 ttcagaaact tcttcaatgc tttttacagt gtggtggaac tggtagagaa ggatggctca
      541 gtgtccagcc tgctgaaggt gttcaacgac cagagtgcct cggaccacat cgtgcagttc
      601 ctgcgcctgc tcacgtcggc cttcatcagg aaccgagcag acttcttccg gcacttcatt
      661 gatgaggaga tggacatcaa agacttctgc actcacgaag tagagcccat ggccacggag
      721 tgtgaccaca tccagatcac ggcgttgtcg caggccctga gcattgccct gcaagtggag
      781 tacgtggacg agatggatac cgccctgaac caccacgtgt tccctgaggc cgccacccct
      841 tccgtttacc tgctctataa aacatcccac tacaacatcc tttatgcagc cgataaacat
      901 tgattaattt taggccatgc agtggaacct gtcacctaat gggactgcat tctgaatgga
      961 acattccggc tcttcaattt tttaagcaat ttagactgta gcaagaaaat gtgcagcctt
     1021 ttgggcaaag cccctgggaa cgaggcctat ccactatgga ctatggtaac ttggtaggat
     1081 ttttagtatt tattttaatg gagggacaaa tgctttctaa ctgggccccc gactccgcac
     1141 cccagttcgc agtgaggccc tgggtgggtc acctgccctc tctggacttg tttcttcaac
     1201 tggaggaggt ccctgcctat gctgacattc cattgtagaa aaatggggcc tctggtgtct
     1261 ctttaccagg ggcagtgcct ctctgcgggg gaggaaaagc tcaaggttag ctgtcttaac
     1321 ccaagtgact taccaggcct acaaaagagt ccagtccagt cacttgttgg actgggctct
     1381 gacagatggc ctattgaggt caacttgaat gtgagggcta cggtgtggtt tcaaacattc
     1441 atgatggatg tattttccta cccctaactt aaggagaaaa aaaaaaagac ttcctttttt
     1501 tgccaaagtc cagaaagggg cctttagcct ttagtaggag ctcaaattgt tggggcccct
     1561 ctacctctct cagggctaga actgcctgac tcttggtgga cgagcccttc agggttctgc
     1621 tttcagcccc acctggacag aggcttacaa gactagggtc tggaccagaa tctgtgtatt
     1681 tctgtctggg accaggaagc cgcagctgtc ccatcatccc cagcaaatcc tagaagtgga
     1741 gtctggatac ttcaaggata gaagtgttgg cacgcacagc catggaccca gctgagcaga
     1801 gcagacgctt tgcaggctgc ccctggcttc ttcctccctt tcccgcttct gctctcttta
     1861 tggactggtc agagggtagg tgggaaagaa cagacaagcc atgggaagtt ggcagtgggg
     1921 agatttccac tgtggaaacc gcctgggaat tccggccagc agcttcctcc ttcagccacc
     1981 tggccatacc ccttaaataa gcccctcacc ttgctgcctc aggaccttca agattccatc
     2041 tgtgggctgg ccggcaagat ggcaccagtg gggacccaca ccctggctgg gcagaggtgc
     2101 tgctagcaac ctctcttcct ctataagagg aaatggaaaa tgcagggtgt ggaattgccc
     2161 tttggggtcc ttccttaatt gaaggccacc ttctcacagg tttcattctg cagggattta
     2221 ctggaatcta ttggtgctgc tgcatgagtc tgctgacaac ctgactgcac aaggactggg
     2281 tagcagactc ctcagagtcc tcttgacaca aatgtcagat ttgtgtcact cttctgcctt
     2341 cgtgaaaagc caatagcact ctcagatatc aggggatttt agttccaagc agggaccctg
     2401 gtttccatac tgccctcagc tggagtttgg atccaaaggc tctggctaag tcattatgtc
     2461 actttttcac aggaatgtaa atttgactgt cacctctgaa tttgttcagt gtcccaccat
     2521 ggtctatgag aagtacactg gaagcgtggg gggaacacat gacatgattt gtgaatatca
     2581 tcatctttgc cagacaagtc tccaggggat ccctgtttcc caactgaaag gtgtgaacgg
     2641 acacacacac agcctggatg acgccttggc tgttctaagg ggctgtaagg tgggctctgg
     2701 gccttccagc taggctctca agcacagcag aagcctcact gggctgctat gtctctgtat
     2761 ttgtggcttg tgtggtagcc tcagaagcag agctgtttgg cagactggct ggagaaattc
     2821 cctctaggag acttgcctgt gctgtgcttc caggtcacag agccccccgg aaactcacag
     2881 gggccctctt cccagaaaag aatctattct atcacttcag aatcaggaca ctcaagctct
     2941 ggcagaggaa ggccaagtta ctttcatggt cttaccctct gcttttcccc tttttgcaaa
     3001 aaaccactgg ccaaatccga accattgccc ttgtttcccc cacgttctct ctcagatctt
     3061 tgtctcgaag ggaaaacata gtggatgaaa aggtgtggca ggctttggca ccttgttaaa
     3121 atttctagtc atctgtggat gttaccttgc ttgtccacag cagccagtca ccctggccag
     3181 tcccacttcc tggataattc tctaccctca ccccacagag ccatctctct ccagaccaaa
     3241 agctggaagg agagttgctt tgagagcttg tttttacaac tgcatgttta ttatgatact
     3301 ttctctccaa aggaaacttt taaatcaatg ggaacaatta gcaacagaaa gagcacagtc
     3361 cctgcttttg actgggttcc tattttaagc acaaatgaga gctctggagc cagaatgcca
     3421 gggttctaac ttcagcattc acttactagc tgtatgatct tggccaagtc acttcacctc
     3481 cctgagcccc aattcccaag tttgtgaaat ggcaacaata cctatgtgtc actggattat
     3541 tggttaaaac agaatgagat tccttgtgtg aaaatagcta ttatacctga cacactcatc
     3601 gtatgggctc tgcaaaggga tattccccaa cctgtccttc ctgacaggaa gcatagggca
     3661 ctgcagatgg ggaagcatgt caccttggca gtgactcggt ggcttcccaa gcaggagtgt
     3721 caggggaacc atgagagaga gtctaggagc aaacacatca ccaccctgag cagatacagg
     3781 agtggggagg gggctgtaac tcagtgagtg gcttccaggg gccccaggcc ctgctggatg
     3841 tgggccaagc cctacagctt ccctaggcag taagtaaaaa cattctccta gcattaaaat
     3901 ggtttccata a
//
