LOCUS       NM_194248               7214 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens otoferlin (OTOF), transcript variant 1, mRNA.
ACCESSION   NM_194248
VERSION     NM_194248.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Wu,K.L., Li,J., Wang,H.Y. and Wang,Q.J.
  TITLE     [The updates of the ACMG variant interpretation guidelines affect
            the pathogenicity determination of OTOF gene variations in patients
            with auditory neuropathy]
  JOURNAL   Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 59 (5), 455-463
            (2024)
   PUBMED   38811176
  REMARK    GeneRIF: [The updates of the ACMG variant interpretation guidelines
            affect the pathogenicity determination of OTOF gene variations in
            patients with auditory neuropathy].
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Dmitriev,D.A., Shilov,B.V., Polunin,M.M., Zadorozhny,A.D. and
            Lagunin,A.A.
  TITLE     Predicting the Impact of OTOF Gene Missense Variants on Auditory
            Neuropathy Spectrum Disorder
  JOURNAL   Int J Mol Sci 24 (24), 17240 (2023)
   PUBMED   38139069
  REMARK    GeneRIF: Predicting the Impact of OTOF Gene Missense Variants on
            Auditory Neuropathy Spectrum Disorder.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7214)
  AUTHORS   Asaad,M., Mahfood,M., Al Mutery,A. and Tlili,A.
  TITLE     Loss-of-function mutations in MYO15A and OTOF cause non-syndromic
            hearing loss in two Yemeni families
  JOURNAL   Hum Genomics 17 (1), 42 (2023)
   PUBMED   37189200
  REMARK    GeneRIF: Loss-of-function mutations in MYO15A and OTOF cause
            non-syndromic hearing loss in two Yemeni families.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7214)
  AUTHORS   Mohammadi,S., Khamirani,H.J., Zoghi,S., Dastgheib,S.A., Bagher
            Tabei,S.M., Talebzadeh,M., Adibi,M.H. and Dianatpour,M.
  TITLE     Intronic OTOF mutation causes an atypical splicing defect resulting
            in auditory neuropathy spectrum disorder
  JOURNAL   J Genet 102 (2023)
   PUBMED   36988134
  REMARK    GeneRIF: Intronic OTOF mutation causes an atypical splicing defect
            resulting in auditory neuropathy spectrum disorder.
REFERENCE   5  (bases 1 to 7214)
  AUTHORS   Yasunaga,S., Grati,M., Cohen-Salmon,M., El-Amraoui,A., Mustapha,M.,
            Salem,N., El-Zir,E., Loiselet,J. and Petit,C.
  TITLE     A mutation in OTOF, encoding otoferlin, a FER-1-like protein,
            causes DFNB9, a nonsyndromic form of deafness
  JOURNAL   Nat Genet 21 (4), 363-369 (1999)
   PUBMED   10192385
  REMARK    GeneRIF: This publication deals with the otoferlin, which is
            related in sequence to FER1L4.
REFERENCE   6  (bases 1 to 7214)
  AUTHORS   Achanzar,W.E. and Ward,S.
  TITLE     A nematode gene required for sperm vesicle fusion
  JOURNAL   J Cell Sci 110 (Pt 9), 1073-1081 (1997)
   PUBMED   9175703
  REMARK    GeneRIF: This publication deals with otoferlin (FER-1), which is
            similar to FER1L4, in both C. elegans and humans.
REFERENCE   7  (bases 1 to 7214)
  AUTHORS   Chaib,H., Place,C., Salem,N., Chardenoux,S., Vincent,C.,
            Weissenbach,J., El-Zir,E., Loiselet,J. and Petit,C.
  TITLE     A gene responsible for a sensorineural nonsyndromic recessive
            deafness maps to chromosome 2p22-23
  JOURNAL   Hum Mol Genet 5 (1), 155-158 (1996)
   PUBMED   8789454
REFERENCE   8  (bases 1 to 7214)
  AUTHORS   Fukushima,K., Ramesh,A., Srisailapathy,C.R., Ni,L., Wayne,S.,
            O'Neill,M.E., Van Camp,G., Coucke,P., Jain,P., Wilcox,E.R.,
            Smith,S.D., Kenyon,J.B., Zbar,R.I. and Smith,R.J.
  TITLE     An autosomal recessive nonsyndromic form of sensorineural hearing
            loss maps to 3p-DFNB6
  JOURNAL   Genome Res 5 (3), 305-308 (1995)
   PUBMED   8593615
REFERENCE   9  (bases 1 to 7214)
  AUTHORS   Shearer,A.E., Hildebrand,M.S., Odell,A.M. and Smith,R.J.H.
  TITLE     Genetic Hearing Loss Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301607
REFERENCE   10 (bases 1 to 7214)
  AUTHORS   Azaiez,H., Thorpe,R.K. and Smith,R.J.H.
  TITLE     OTOF-Related Deafness
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301429
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC108070.2, AF183185.1,
            AC093378.5 and AF183187.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_194248.2.
            
            Summary: Mutations in this gene are a cause of neurosensory
            nonsyndromic recessive deafness, DFNB9. The short form of the
            encoded protein has 3 C2 domains, a single carboxy-terminal
            transmembrane domain found also in the C. elegans spermatogenesis
            factor FER-1 and human dysferlin, while the long form has 6 C2
            domains. The homology suggests that this protein may be involved in
            vesicle membrane fusion. Several transcript variants encoding
            multiple isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF183185.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000272371.7/ ENSP00000272371.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                AC108070.2         80409-80466         c
            59-6484             AF183185.1         1-6426
            6485-7174           AC093378.5         40802-41491         c
            7175-7214           AF183187.1         4718-4757
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p23.3"
     gene            1..7214
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /note="otoferlin"
                     /db_xref="GeneID:9381"
                     /db_xref="HGNC:HGNC:8515"
                     /db_xref="MIM:603681"
     exon            1..264
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     CDS             186..6179
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /note="isoform a is encoded by transcript variant 1;
                     fer-1-like protein 2; fer-1-like family member 2"
                     /codon_start=1
                     /product="otoferlin isoform a"
                     /protein_id="NP_919224.1"
                     /db_xref="CCDS:CCDS1725.1"
                     /db_xref="GeneID:9381"
                     /db_xref="HGNC:HGNC:8515"
                     /db_xref="MIM:603681"
                     /translation="MALLIHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENCEDVADF
                     DETFRWPVASSIDRNEMLEIQVFNYSKVFSNKLIGTFRMVLQKVVEESHVEVTDTLID
                     DNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSS
                     RPPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRPDEPAVLEMEDLDHLAIRLGDGLDP
                     DSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVC
                     VEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLV
                     GSFKMDVGTVYSQPEHQFHHKWAILSDPDDISSGLKGYVKCDVAVVGKGDNIKTPHKA
                     NETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKD
                     LVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDV
                     AIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFR
                     ARLLLGLAVEIVDTSNPELTSSTEVQVEQATPISESCAGKMEEFFLFGAFLEASMIDR
                     RNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNASDDEAGDAGDL
                     ASVSSTPPMRPQVTDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEE
                     GLNDIQEMIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSC
                     MRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSN
                     NKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYL
                     WLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSL
                     FAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPP
                     IIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDL
                     LAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLK
                     RVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNI
                     RVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTVRLLRRCRVLCNGGSSSH
                     STGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEE
                     PDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAA
                     KEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKT
                     GDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLV
                     RVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFP
                     MESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPM
                     KPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLAL
                     RHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISP
                     RKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLT
                     GEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADD
                     FLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDE
                     FELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFL
                     WHTYRWLLLKLLLLLLLLLLLALFLYSVPGYLVKKILGA"
     exon            265..323
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            324..412
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            413..512
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            513..694
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            695..768
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            769..895
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            896..950
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1082
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1145
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1230
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1390
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1577
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1764
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1988
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2097
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2278
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2399
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            2400..2500
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2591
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            2592..2708
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            2709..2861
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            2862..3051
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            3052..3176
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            3177..3311
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            3312..3473
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            3474..3593
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            3594..3755
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            3756..3918
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            3919..4049
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            4050..4079
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            4080..4208
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            4209..4275
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            4276..4412
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            4413..4547
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            4548..4685
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            4686..4813
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            4814..4984
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            4985..5145
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            5146..5288
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            5289..5377
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            5378..5476
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            5477..5718
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            5719..5897
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            5898..5998
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            5999..6196
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     exon            6197..7214
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7193..7198
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /note="hexamer: AATAAA"
     polyA_site      7214
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /note="major polyA site"
ORIGIN      
        1 gtgagtcgct gcaggagcag cttagctcgg cttggttgcc ttggtctctg tgggcagcat
       61 cggagggggg tcgggaggag gaggaggagg cagcggcaga gaagagagag gcgtgtgagc
      121 cgtgctccac cggctagctc cttcccgctg ctcctgcctg gcagtgccag gcagcccaca
      181 ccagcatggc cttgctcatc cacctcaaga cagtctcgga gctgcggggc aggggcgacc
      241 ggatcgccaa agtgactttc cgagggcaat ccttctactc tcgggtcctg gagaactgtg
      301 aggatgtggc tgactttgat gagacatttc ggtggccggt ggccagcagc atcgacagaa
      361 atgagatgct ggagattcag gttttcaact acagcaaagt cttcagcaac aagctcatcg
      421 ggaccttccg catggtgctg cagaaggtgg tagaggagag ccatgtggag gtgactgaca
      481 cgctgattga tgacaacaat gctatcatca agaccagcct gtgcgtggag gtccggtatc
      541 aggccactga cggcacagtg ggctcctggg acgatgggga cttcctggga gatgagtctc
      601 ttcaagagga agagaaggac agccaagaga cggatggact gctcccaggc tcccggccca
      661 gctcccggcc cccaggagag aagagcttcc ggagagccgg gaggagcgtg ttctccgcca
      721 tgaagctcgg caaaaaccgg tctcacaagg aggagcccca aagaccagat gaaccggcgg
      781 tgctggagat ggaagacctt gaccatctgg ccattcggct aggagatgga ctggatcccg
      841 actcggtgtc tctagcctca gtcacagctc tcaccactaa tgtctccaac aagcgatcta
      901 agccagacat taagatggag ccaagtgctg ggcggcccat ggattaccag gtcagcatca
      961 cggtgatcga ggcccggcag ctggtgggct tgaacatgga ccctgtggtg tgcgtggagg
     1021 tgggtgacga caagaagtac acatccatga aggagtccac taactgcccc tattacaacg
     1081 agtacttcgt cttcgacttc catgtctctc cggatgtcat gtttgacaag atcatcaaga
     1141 tttcggtgat tcactccaag aacctgctgc gcagtggcac cctggtgggc tccttcaaaa
     1201 tggacgtggg aaccgtgtac tcgcagccag agcaccagtt ccatcacaag tgggccatcc
     1261 tgtctgaccc cgatgacatc tcctcggggc tgaagggcta cgtgaagtgt gacgttgccg
     1321 tggtgggcaa aggggacaac atcaagacgc cccacaaggc caatgagacc gacgaagatg
     1381 acattgaggg gaacttgctg ctccccgagg gggtgccccc cgaacgccag tgggcccggt
     1441 tctatgtgaa aatttaccga gcagaggggc tgccccgtat gaacacaagc ctcatggcca
     1501 atgtaaagaa ggctttcatc ggtgaaaaca aggacctcgt ggacccctac gtgcaagtct
     1561 tctttgctgg ccagaagggc aagacttcag tgcagaagag cagctatgag cccctgtgga
     1621 atgagcaggt cgtctttaca gacctcttcc ccccactctg caaacgcatg aaggtgcaga
     1681 tccgagactc ggacaaggtc aacgacgtgg ccatcggcac ccacttcatt gacctgcgca
     1741 agatttctaa tgacggagac aaaggcttcc tgcccacact gggcccagcc tgggtgaaca
     1801 tgtacggctc cacacgtaac tacacgctgc tggatgagca tcaggacctg aacgagggcc
     1861 tgggggaggg tgtgtccttc cgggcccggc tcctgctggg cctggctgtg gagatcgtag
     1921 acacctccaa ccctgagctc accagctcca cagaggtgca ggtggagcag gccacgccca
     1981 tctcggagag ctgtgcaggt aaaatggaag aattctttct ctttggagcc ttcctggagg
     2041 cctcaatgat cgaccggaga aacggagaca agcccatcac ctttgaggtc accataggca
     2101 actatgggaa cgaagttgat ggcctgtccc ggccccagcg gcctcggccc cggaaggagc
     2161 cgggggatga ggaagaagta gacctgattc agaacgcaag tgatgacgag gccggtgatg
     2221 ccggggacct ggcctcagtc tcctccactc caccaatgcg gccccaggtc accgacagga
     2281 actacttcca tctgccctac ctggagcgaa agccctgcat ctacatcaag agctggtggc
     2341 cggaccagcg ccgccgcctc tacaatgcca acatcatgga ccacattgcc gacaagctgg
     2401 aagaaggcct gaacgacata caggagatga tcaaaacgga gaagtcctac cctgagcgtc
     2461 gcctgcgggg cgtcctggag gagctgagct gtggctgctg ccgcttcctc tccctcgctg
     2521 acaaggacca gggccactca tcccgcacca ggcttgaccg ggagcgcctc aagtcctgca
     2581 tgagggagct ggaaaacatg gggcagcagg ccaggatgct gcgggcccag gtgaagcggc
     2641 acacggtgcg ggacaagctg aggctgtgcc agaacttcct gcagaagctg cgcttcctgg
     2701 cggacgagcc ccagcacagc attcccgaca tcttcatctg gatgatgagc aacaacaagc
     2761 gtgtcgccta tgcccgtgtg ccctccaagg acctgctctt ctccatcgtg gaggaggaga
     2821 ctggcaagga ctgcgccaag gtcaagacgc tcttccttaa gctgccaggg aagcggggct
     2881 tcggctcggc aggctggaca gtgcaggcca aggtggagct gtacctgtgg ctgggcctca
     2941 gcaaacagcg caaggagttc ctgtgcggcc tgccctgtgg cttccaggag gtcaaggcag
     3001 cccagggcct gggcctgcat gccttcccac ccgtcagcct ggtctacacc aagaagcagg
     3061 cgttccagct ccgagcgcac atgtaccagg cccgcagcct ctttgccgcc gacagcagcg
     3121 gactctcaga cccctttgcc cgcgtcttct tcatcaatca gagtcagtgc acagaggtgc
     3181 tgaatgagac cctgtgtccc acctgggacc agatgctggt gttcgacaac ctggagctct
     3241 atggtgaagc tcatgagctg agggacgatc cgcccatcat tgtcattgaa atctatgacc
     3301 aggattccat gggcaaagct gacttcatgg gccggacctt cgccaaaccc ctggtgaaga
     3361 tggcagacga ggcgtactgc ccaccccgct tcccacctca gctcgagtac taccagatct
     3421 accgtggcaa cgccacagct ggagacctgc tggcggcctt cgagctgctg cagattggac
     3481 cagcagggaa ggctgacctg ccccccatca atggcccggt ggacgtggac cgaggtccca
     3541 tcatgcccgt gcccatgggc atccggcccg tgctcagcaa gtaccgagtg gaggtgctgt
     3601 tctggggcct acgggaccta aagcgggtga acctggccca ggtggaccgg ccacgggtgg
     3661 acatcgagtg tgcagggaag ggggtgcagt cgtccctgat ccacaattat aagaagaacc
     3721 ccaacttcaa caccctcgtc aagtggtttg aagtggacct cccagagaac gagctgctgc
     3781 acccgccctt gaacatccgt gtggtggact gccgggcctt cggtcgctac acactggtgg
     3841 gctcccatgc cgtcagctcc ctgcgacgct tcatctaccg gcccccagac cgctcggccc
     3901 ccagctggaa caccacggtc aggcttctcc ggcgctgccg tgtgctgtgc aatgggggct
     3961 cctcctctca ctccacaggg gaggttgtgg tgactatgga gccagaggta cccatcaaga
     4021 aactggagac catggtgaag ctggacgcga cttctgaagc tgttgtcaag gtggatgtgg
     4081 ctgaggagga gaaggagaag aagaagaaga agaagggcac tgcggaggag ccagaggagg
     4141 aggagccaga cgagagcatg ctggactggt ggtccaagta ctttgcctcc attgacacca
     4201 tgaaggagca acttcgacaa caagagccct ctggaattga cttggaggag aaggaggaag
     4261 tggacaatac cgagggcctg aaggggtcaa tgaagggcaa ggagaaggca agggctgcca
     4321 aagaggagaa gaagaagaaa actcagagct ctggctctgg ccaggggtcc gaggcccccg
     4381 agaagaagaa acccaagatt gatgagctta aggtataccc caaagagctg gagtccgagt
     4441 ttgataactt tgaggactgg ctgcacactt tcaacttgct tcggggcaag accggggatg
     4501 atgaggatgg ctccaccgag gaggagcgca ttgtgggacg cttcaagggc tccctctgcg
     4561 tgtacaaagt gccactccca gaggacgtgt cccgggaagc cggctacgac tccacctacg
     4621 gcatgttcca gggcatcccg agcaatgacc ccatcaatgt gctggtccga gtctatgtgg
     4681 tccgggccac ggacctgcac cctgctgaca tcaacggcaa agctgacccc tacatcgcca
     4741 tccggctagg caagactgac atccgcgaca aggagaacta catctccaag cagctcaacc
     4801 ctgtctttgg gaagtccttt gacatcgagg cctccttccc catggaatcc atgctgacgg
     4861 tggctgtgta tgactgggac ctggtgggca ctgatgacct cattggggaa accaagatcg
     4921 acctggagaa ccgcttctac agcaagcacc gcgccacctg cggcatcgcc cagacctact
     4981 ccacacatgg ctacaatatc tggcgggacc ccatgaagcc cagccagatc ctgacccgcc
     5041 tctgcaaaga cggcaaagtg gacggccccc actttgggcc ccctgggaga gtgaaggtgg
     5101 ccaaccgcgt cttcactggg ccctctgaga ttgaggacga gaacggtcag aggaagccca
     5161 cagacgagca tgtggcgctg ttggccctga ggcactggga ggacatcccc cgcgcaggct
     5221 gccgcctggt gccagagcat gtggagacga ggccgctgct caaccccgac aagccgggca
     5281 tcgagcaggg ccgcctggag ctgtgggtgg acatgttccc catggacatg ccagcccctg
     5341 ggacgcctct ggacatctca cctcggaagc ccaagaagta cgagctgcgg gtcatcatct
     5401 ggaacacaga tgaggtggtc ttggaggacg acgacttctt cacaggggag aagtccagtg
     5461 acatcttcgt gagggggtgg ctgaagggcc agcaggagga caagcaggac acagacgtcc
     5521 actaccactc cctcactggc gagggcaact tcaactggcg ctacctgttc cccttcgact
     5581 acctggcggc ggaggagaag atcgtcatct ccaagaagga gtccatgttc tcctgggacg
     5641 agaccgagta caagatcccc gcgcggctca ccctgcagat ctgggatgcg gaccacttct
     5701 ccgctgacga cttcctgggg gccatcgagc tggacctgaa ccggttcccg cggggcgcaa
     5761 agacagccaa gcagtgcacc atggagatgg ccaccgggga ggtggacgtg cccctcgtgt
     5821 ccatcttcaa gcaaaagcgc gtcaaaggct ggtggcccct cctggcccgc aatgagaacg
     5881 atgagtttga gctcacgggc aaggtggagg ctgagctgca tttactgaca gcagaggagg
     5941 cagagaagaa cccagtgggc ctggcccgca atgaacctga ccccctagag aaacccaacc
     6001 ggcccgacac gagcttcatc tggttcctga accctctcaa gtcggctcgc tacttcttgt
     6061 ggcacacgta tcgctggctg ctcctcaaac tgttgctgct cctgctgctg ctcctcctcc
     6121 tcgccctgtt cctctactct gtgcctggct acctggtcaa gaaaatcctc ggggcctgag
     6181 cccagtggcc tcctggccgg cccgacacgg ccttcgtctg gttcctcaac cctctcaagt
     6241 ccatcaagta cctcatctgc acccggtaca agtggctcat catcaagatc gtgctggcgc
     6301 tgttggggct gctcatgttg gggctcttcc tctacagcct ccctggctac atggtcaaaa
     6361 agctccttgg ggcatgaagg ccgccagctc ccgccagccg ctccccagcc ctgccgcatt
     6421 tcctttcagt ggcttggact ctttcccatc tcccctgggg agcctgagga gcccagcgtc
     6481 cactcttcat gccttgggcc gagcctgcct cctgcttgcg ggggccgcct gtcctcactg
     6541 ccccaggctg cggcttgccc agtcccgccc ctctgacccc tgcctgtggg ctggggagcc
     6601 ttggatgggg tggggacctg gaatgggtct ctcttgcccc acctggctga ggcgccaccc
     6661 ttcttcaggc ccaggctcca gaggaagact cctgaaaccc tccccaggtc ttccaagtac
     6721 aggattgaag ctttagtgaa attaaccaag gaccatgggt cagtgcccag ggctttaaaa
     6781 agaatgaacg agcaaaaggt atccccgccg tgacccctgc agatagcacc ggtctttgat
     6841 ccgcagcagg ggccagaccc tgcccacaag tcccagcgcg gctgcttctg ccactgctgg
     6901 gctccacttg gctcctctca cttcccaggg ggtcgcctgt cctgcctgtg ggtttccatg
     6961 gcttcccaga gctccctctg ccccagccag cgcctccagg cccagctgag gagctgtgag
     7021 aagcagcaga ggggactccc catcccgggc acaccctgtc ctcccacccc tgcccccttg
     7081 cccttccagc cctttcagct gcagctggga gctggcccgt caagtgctgc ccctgcctgt
     7141 gtctgggttt ctgttggctg tttttctttt cttgagtggt gatttttctc taaataaaag
     7201 aagtcaagca ctga
//
