LOCUS       NM_021227               1062 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens oligosaccharyltransferase complex non-catalytic
            subunit (OSTC), transcript variant 1, mRNA.
ACCESSION   NM_021227
VERSION     NM_021227.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Lampson,B.L., Ramiotarez,A.S., Baro,M., He,L., Hegde,M., Koduri,V.,
            Pfaff,J.L., Hanna,R.E., Kowal,J., Shirole,N.H., He,Y., Doench,J.G.,
            Contessa,J.N., Locher,K.P. and Kaelin,W.G. Jr.
  TITLE     Positive selection CRISPR screens reveal a druggable pocket in an
            oligosaccharyltransferase required for inflammatory signaling to
            NF-kappaB
  JOURNAL   Cell 187 (9), 2209-2223 (2024)
   PUBMED   38670073
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Gemmer,M., Chaillet,M.L., van Loenhout,J., Cuevas Arenas,R.,
            Vismpas,D., Grollers-Mulderij,M., Koh,F.A., Albanese,P.,
            Scheltema,R.A., Howes,S.C., Kotecha,A., Fedry,J. and Forster,F.
  TITLE     Visualization of translation and protein biogenesis at the ER
            membrane
  JOURNAL   Nature 614 (7946), 160-167 (2023)
   PUBMED   36697828
REFERENCE   3  (bases 1 to 1062)
  AUTHORS   Bryant,E.M., Millichap,J.J., Spinelli,E., Calhoun,J.D., Miller,C.,
            Giannelli,J., Wolak,J., Sanders,V., Carvill,G.L. and Charrow,J.
  TITLE     Oligosaccharyltransferase complex-congenital disorders of
            glycosylation: A novel congenital disorder of glycosylation
  JOURNAL   Am J Med Genet A 182 (6), 1460-1465 (2020)
   PUBMED   32267060
  REMARK    GeneRIF: Oligosaccharyltransferase complex-congenital disorders of
            glycosylation: A novel congenital disorder of glycosylation.
REFERENCE   4  (bases 1 to 1062)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1062)
  AUTHORS   Ramirez,A.S., Kowal,J. and Locher,K.P.
  TITLE     Cryo-electron microscopy structures of human
            oligosaccharyltransferase complexes OST-A and OST-B
  JOURNAL   Science 366 (6471), 1372-1375 (2019)
   PUBMED   31831667
REFERENCE   6  (bases 1 to 1062)
  AUTHORS   Shrimal,S., Cherepanova,N.A. and Gilmore,R.
  TITLE     DC2 and KCP2 mediate the interaction between the
            oligosaccharyltransferase and the ER translocon
  JOURNAL   J Cell Biol 216 (11), 3625-3638 (2017)
   PUBMED   28860277
  REMARK    GeneRIF: DC2 and KCP2 mediate the interaction between the
            oligosaccharyltransferase STT3A and the endoplasmic reticulum
            translocon.
REFERENCE   7  (bases 1 to 1062)
  AUTHORS   Chen,C.M., Tseng,C.N., Cho,J.J., Lee,Y.Z., Kao,C.L., Cheng,Y.B.,
            Hong,Y.R. and Cho,C.L.
  TITLE     Heat shock induces expression of OSTC/DC2, a novel subunit of
            oligosaccharyltransferase, in vitro and in vivo
  JOURNAL   Kaohsiung J Med Sci 30 (5), 219-223 (2014)
   PUBMED   24751383
  REMARK    GeneRIF: These results suggest that the upregulation of OSTC/DC2, a
            novel component of the oligosaccharyltransferase complex, is part
            of the mammalian heat shock response.
REFERENCE   8  (bases 1 to 1062)
  AUTHORS   Kalsi,G., Kuo,P.H., Aliev,F., Alexander,J., McMichael,O.,
            Patterson,D.G., Walsh,D., Zhao,Z., Schuckit,M., Nurnberger,J. Jr.,
            Edenberg,H., Kramer,J., Hesselbrock,V., Tischfield,J.A.,
            Vladimirov,V., Prescott,C.A., Dick,D.M., Kendler,K.S. and
            Riley,B.P.
  TITLE     A systematic gene-based screen of chr4q22-q32 identifies
            association of a novel susceptibility gene, DKK2, with the
            quantitative trait of alcohol dependence symptom counts
  JOURNAL   Hum Mol Genet 19 (12), 2497-2506 (2010)
   PUBMED   20332099
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Hum Mol Genet. 2010 Oct 15;19(20):4121. Hesselbrock,
            Victor [added]; Tischfield, Jay A [added]]
REFERENCE   9  (bases 1 to 1062)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
REFERENCE   10 (bases 1 to 1062)
  AUTHORS   Shibatani,T., David,L.L., McCormack,A.L., Frueh,K. and Skach,W.R.
  TITLE     Proteomic analysis of mammalian oligosaccharyltransferase reveals
            multiple subcomplexes that contain Sec61, TRAP, and two potential
            new subunits
  JOURNAL   Biochemistry 44 (16), 5982-5992 (2005)
   PUBMED   15835887
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC054857.1 and BM994257.1.
            
            On Nov 23, 2018 this sequence version replaced NM_021227.3.
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF161425.1, BX354694.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361564.9/ ENSP00000354676.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1058              BC054857.1         5-1062
            1059-1062           BM994257.1         17-20               c
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q25"
     gene            1..1062
                     /gene="OSTC"
                     /gene_synonym="DC2"
                     /note="oligosaccharyltransferase complex non-catalytic
                     subunit"
                     /db_xref="GeneID:58505"
                     /db_xref="HGNC:HGNC:24448"
                     /db_xref="MIM:619023"
     exon            1..196
                     /gene="OSTC"
                     /gene_synonym="DC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..507
                     /gene="OSTC"
                     /gene_synonym="DC2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     oligosaccharyltransferase complex subunit OSTC;
                     hydrophobic protein HSF-28; oligosaccharyltransferase
                     complex subunit (non-catalytic)"
                     /codon_start=1
                     /product="oligosaccharyltransferase complex subunit OSTC
                     isoform 1"
                     /protein_id="NP_067050.1"
                     /db_xref="CCDS:CCDS3681.1"
                     /db_xref="GeneID:58505"
                     /db_xref="HGNC:HGNC:24448"
                     /db_xref="MIM:619023"
                     /translation="METLYRVPFLVLECPNLKLKKPPWLHMPSAMTVYALVVVSYFLI
                     TGGIIYDVIVEPPSVGSMTDEHGHQRPVAFLAYRVNGQYIMEGLASSFLFTMGGLGFI
                     ILDRSNAPNIPKLNRFLLLFIGFVCVLLSFFMARVFMRMKLPGYLMG"
     exon            197..290
                     /gene="OSTC"
                     /gene_synonym="DC2"
                     /inference="alignment:Splign:2.1.0"
     exon            291..488
                     /gene="OSTC"
                     /gene_synonym="DC2"
                     /inference="alignment:Splign:2.1.0"
     exon            489..1062
                     /gene="OSTC"
                     /gene_synonym="DC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1041..1046
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OSTC"
                     /gene_synonym="DC2"
                     /note="hexamer: AATAAA"
     polyA_site      1062
                     /gene="OSTC"
                     /gene_synonym="DC2"
                     /note="major polyA site"
ORIGIN      
        1 ctgtttccgg gaggcgcgtg gggcttgagg ccgagaacgg cccttgctgc caccaacatg
       61 gagactttgt accgtgtccc gttcttagtg ctcgaatgtc ccaacctgaa gctgaagaag
      121 ccgccctggt tgcacatgcc gtcggccatg actgtgtatg ctctggtggt ggtgtcttac
      181 ttcctcatca ccggaggaat aatttatgat gttattgttg aacctccaag tgtcggttct
      241 atgactgatg aacatgggca tcagaggcca gtagctttct tggcctacag agtaaatgga
      301 caatatatta tggaaggact tgcatccagc ttcctattta caatgggagg tttaggtttc
      361 ataatcctgg accgatcgaa tgcaccaaat atcccaaaac tcaatagatt ccttcttctg
      421 ttcattggat tcgtctgtgt cctattgagt tttttcatgg ctagagtatt catgagaatg
      481 aaactgccgg gctatctgat gggttagagt gcctttgaga agaaatcagt ggatactgga
      541 tttgctcctg tcaatgaagt tttaaaggct gtaccaatcc tctaatatga aatgtggaaa
      601 agaatgaaga gcagcagtaa aagaaatatc tagtgaaaaa acaggaagcg tattgaagct
      661 tggactagaa tttcttcttg gtattaaaga gacaagttta tcacagaatt ttttttcctg
      721 ctggcctatt gctataccaa tgatgttgag tggcattttc tttttagttt ttcattaaaa
      781 tatattccat atctacaact ataatatcaa ataaagtgat tattttttac aaccctctta
      841 acattttttg gagatgacat ttctgatttt cagaaattaa cataaaatcc agaagcaaga
      901 ttccgtaagc tgagaactct ggacagttga tcagctttac ctatggtgct ttgcctttaa
      961 ctagagtgtg tgatggtaga ttatttcaga tatgtatgta aaactgtttc ctgaacaata
     1021 agatgtatga acggagcaga aataaatact ttttctaatt aa
//
