LOCUS       NM_024586               3660 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens oxysterol binding protein like 9 (OSBPL9), transcript
            variant 6, mRNA.
ACCESSION   NM_024586
VERSION     NM_024586.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Hacker,B., Schultheiss,C., Doring,M. and Kurzik-Dumke,U.
  TITLE     Molecular partners of hNOT/ALG3, the human counterpart of the
            Drosophila NOT and yeast ALG3 gene, suggest its involvement in
            distinct cellular processes relevant to congenital disorders of
            glycosylation, cancer, neurodegeneration and a variety of further
            pathologies
  JOURNAL   Hum Mol Genet 27 (11), 1858-1878 (2018)
   PUBMED   29547901
REFERENCE   3  (bases 1 to 3660)
  AUTHORS   Liu,X. and Ridgway,N.D.
  TITLE     Characterization of the sterol and phosphatidylinositol 4-phosphate
            binding properties of Golgi-associated OSBP-related protein 9
            (ORP9)
  JOURNAL   PLoS One 9 (9), e108368 (2014)
   PUBMED   25255026
  REMARK    GeneRIF: These studies identify ORP9 as a dual sterol/PI-4P binding
            protein that could regulate PI-4P in the Golgi apparatus.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3660)
  AUTHORS   Li,L., Qu,G., Wang,M., Huang,Q. and Liu,Y.
  TITLE     Association of oxysterol binding protein-related protein 9
            polymorphism with cerebral infarction in Hunan Han population
  JOURNAL   Ir J Med Sci 183 (3), 439-448 (2014)
   PUBMED   24190612
  REMARK    GeneRIF: T allele of rs768529 may be a risk factor for the
            formation of the carotid vulnerable plaque in Chinese Hunan Han
            population
REFERENCE   5  (bases 1 to 3660)
  AUTHORS   Nissila,E., Ohsaki,Y., Weber-Boyvat,M., Perttila,J., Ikonen,E. and
            Olkkonen,V.M.
  TITLE     ORP10, a cholesterol binding protein associated with microtubules,
            regulates apolipoprotein B-100 secretion
  JOURNAL   Biochim Biophys Acta 1821 (12), 1472-1484 (2012)
   PUBMED   22906437
REFERENCE   6  (bases 1 to 3660)
  AUTHORS   Gauci,S., Helbig,A.O., Slijper,M., Krijgsveld,J., Heck,A.J. and
            Mohammed,S.
  TITLE     Lys-N and trypsin cover complementary parts of the phosphoproteome
            in a refined SCX-based approach
  JOURNAL   Anal Chem 81 (11), 4493-4501 (2009)
   PUBMED   19413330
  REMARK    GeneRIF: Includes data showing N-alpha terminal acetylation of this
            protein (OSBL9_HUMAN), which begins with nASIMEGPLSK following
            cleavage of the initiating Met-1 residue.
REFERENCE   7  (bases 1 to 3660)
  AUTHORS   Lessmann,E., Ngo,M., Leitges,M., Minguet,S., Ridgway,N.D. and
            Huber,M.
  TITLE     Oxysterol-binding protein-related protein (ORP) 9 is a PDK-2
            substrate and regulates Akt phosphorylation
  JOURNAL   Cell Signal 19 (2), 384-392 (2007)
   PUBMED   16962287
  REMARK    GeneRIF: Furthermore, mammalian target of rapamycin was implicated
            in ORP9L phosphorylation in HEK293 cells. These studies identify
            ORP9 as a PDK-2 substrate and negative regulator of Akt
            phosphorylation at the PDK-2 site.
REFERENCE   8  (bases 1 to 3660)
  AUTHORS   Wyles,J.P. and Ridgway,N.D.
  TITLE     VAMP-associated protein-A regulates partitioning of
            oxysterol-binding protein-related protein-9 between the endoplasmic
            reticulum and Golgi apparatus
  JOURNAL   Exp Cell Res 297 (2), 533-547 (2004)
   PUBMED   15212954
REFERENCE   9  (bases 1 to 3660)
  AUTHORS   Jaworski,C.J., Moreira,E., Li,A., Lee,R. and Rodriguez,I.R.
  TITLE     A family of 12 human genes containing oxysterol-binding domains
  JOURNAL   Genomics 78 (3), 185-196 (2001)
   PUBMED   11735225
REFERENCE   10 (bases 1 to 3660)
  AUTHORS   Lehto,M., Laitinen,S., Chinetti,G., Johansson,M., Ehnholm,C.,
            Staels,B., Ikonen,E. and Olkkonen,V.M.
  TITLE     The OSBP-related protein family in humans
  JOURNAL   J Lipid Res 42 (8), 1203-1213 (2001)
   PUBMED   11483621
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB081133.1, AF392445.1,
            AL050343.17 and EL953716.1.
            
            On Nov 22, 2018 this sequence version replaced NM_024586.5.
            
            Summary: This gene encodes a member of the oxysterol-binding
            protein (OSBP) family, a group of intracellular lipid receptors.
            Most members contain an N-terminal pleckstrin homology domain and a
            highly conserved C-terminal OSBP-like sterol-binding domain,
            although some members contain only the sterol-binding domain. This
            family member functions as a cholesterol transfer protein that
            regulates Golgi structure and function. Multiple transcript
            variants, most of which encode distinct isoforms, have been
            identified. Related pseudogenes have been identified on chromosomes
            3, 11 and 12. [provided by RefSeq, Jul 2010].
            
            Transcript Variant: This variant (6) differs in the 5' UTR and 5'
            coding region, uses an alternate start codon, and includes an
            additional in-frame exon in its central coding region, compared to
            variant 1. The encoded isoform (e) has a longer and distinct
            N-terminus, and contains an additional internal segment, compared
            to isoform a. Isoform e contains both a pleckstrin homology domain
            and a sterol-binding domain.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments. This
            transcript contains a uAUG that would result in an 18 aa N-terminal
            extension, but it has a weak Kozak signal, is not supported by the
            vast majority of human and homologous transcripts, and dAUG use is
            supported by data in PMID:19413330, which selectively purifies
            acetylated protein N-termini and shows that Ala-2 is acetylated
            following cleavage of Met-1 as encoded by the dAUG.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.19453.1,
                                           SRR1660805.123569.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: experimental evidence
                                                (PMID:19413330)
            MANE Ensembl match               :: ENST00000428468.6/
                                                ENSP00000407168.1
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                DB081133.1         291-307
            18-2910             AF392445.1         10-2902
            2911-3511           AL050343.17        43301-43901         c
            3512-3660           EL953716.1         37-185              c
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p32.3"
     gene            1..3660
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /note="oxysterol binding protein like 9"
                     /db_xref="GeneID:114883"
                     /db_xref="HGNC:HGNC:16386"
                     /db_xref="MIM:606737"
     exon            1..130
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..2230
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /note="isoform e is encoded by transcript variant 6;
                     oxysterol-binding protein-related protein 9; OSBP-related
                     protein 9"
                     /codon_start=1
                     /product="oxysterol-binding protein-related protein 9
                     isoform e"
                     /protein_id="NP_078862.4"
                     /db_xref="CCDS:CCDS41332.3"
                     /db_xref="GeneID:114883"
                     /db_xref="HGNC:HGNC:16386"
                     /db_xref="MIM:606737"
                     /translation="MASIMEGPLSKWTNVMKGWQYRWFVLDYNAGLLSYYTSKDKMMR
                     GSRRGCVRLRGAVIGIDDEDDSTFTITVDQKTFHFQARDADEREKWIHALEETILRHT
                     LQLQGLDSGFVPSVQDFDKKLTEADAYLQILIEQLKLFDDKLQNCKEDEQRKKIETLK
                     ETTNSMVESIKHCIVLLQIAKDQSNAEKHADGMISTINPVDAIYQPSPLEPVISTMPS
                     QTVLPPEPVQLCKSEQRPSSLPVGPVLATLGHHQTPTPNSTGSGHSPPSSSLTSPSHV
                     NLSPNTVPEFSYSSSEDEFYDADEFHQSGSSPKRLIDSSGSASVLTHSSSGNSLKRPD
                     TTESLNSSLSNGTSDADLFDSHDDRDDDAEAGSVEEHKSVIMHLLSQVRLGMDLTKVV
                     LPTFILERRSLLEMYADFFAHPDLFVSISDQKDPKDRMVQVVKWYLSAFHAGRKGSVA
                     KKPYNPILGEIFQCHWTLPNDTEENTELVSEGPVPWVSKNSVTFVAEQVSHHPPISAF
                     YAECFNKKIQFNAHIWTKSKFLGMSIGVHNIGQGCVSCLDYDEHYILTFPNGYGRSIL
                     TVPWVELGGECNINCSKTGYSANIIFHTKPFYGGKKHRITAEIFSPNDKKSFCSIEGE
                     WNGVMYAKYATGENTVFVDTKKLPIIKKKVRKLEDQNEYESRSLWKDVTFNLKIRDID
                     AATEAKHRLEERQRAEARERKEKEIQWETRLFHEDGECWVYDEPLLKRLGAAKH"
     exon            131..181
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            182..260
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            261..337
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            338..433
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            434..481
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            482..511
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            512..562
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            563..601
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            602..692
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            693..797
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            798..957
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1070
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1189
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1275
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1447
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1532
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1643
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            1644..1707
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            1708..1848
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..1927
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2019
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2155
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     exon            2156..3660
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2817..2822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /note="hexamer: ATTAAA"
     polyA_site      2839
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /note="major polyA site"
     regulatory      2877..2882
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /note="hexamer: CATAAA"
     polyA_site      2910
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /note="major polyA site"
     regulatory      3639..3644
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
                     /note="hexamer: AATACA"
     polyA_site      3660
                     /gene="OSBPL9"
                     /gene_synonym="ORP-9; ORP9"
ORIGIN      
        1 gtcattggcg gctcccaaga tggcgtccat catggaaggg ccgctgagca aatggactaa
       61 cgtgatgaag ggctggcagt accgttggtt cgtgctggac tacaatgcag gactgctctc
      121 ctactacacg tccaaggaca aaatgatgag aggctctcgc agaggatgtg ttagactcag
      181 aggagctgtg attggtatag acgatgagga cgacagcacc ttcacaataa ctgttgatca
      241 gaaaaccttc catttccagg cccgtgatgc tgatgagcga gagaagtgga tccatgcctt
      301 agaagaaaca attcttcgac atactctcca gcttcaaggt ttggattcag gatttgttcc
      361 tagtgtccaa gattttgata agaaacttac agaagctgat gcttacctac aaatcttgat
      421 tgaacaatta aagctttttg atgacaagct tcaaaactgc aaagaagatg aacagagaaa
      481 gaaaattgaa actctcaaag agacaacaaa tagcatggta gaatcaatta aacactgcat
      541 tgtgttgctg cagattgcca aagaccagag taatgcggag aagcacgcag atggaatgat
      601 aagtactatt aatcccgtag atgcaatata tcaacctagt cctttggaac ctgtgatcag
      661 cacaatgcct tcccagactg tgttacctcc agaacctgtt cagttgtgta agtcagagca
      721 gcgtccatct tccctaccag ttggacctgt gttggctacc ttgggacatc atcagactcc
      781 tacaccaaat agtacaggca gtggccattc accaccgagt agcagtctca cttctccaag
      841 ccacgtgaac ttgtctccaa atacagtccc agagttctct tactccagca gtgaagatga
      901 attttatgat gctgatgaat tccatcaaag tggctcatcc ccaaagcgct taatagattc
      961 ttctggatct gcctcagtcc tgacacacag cagctcggga aatagtctaa aacgcccaga
     1021 taccacagaa tcacttaatt cttccttgtc caatggaaca agtgatgctg acctgtttga
     1081 ttcacatgat gacagagatg atgatgcgga ggcagggtct gtggaggagc acaagagcgt
     1141 tatcatgcat ctcttgtcgc aggttagact tggaatggat cttactaagg tagttcttcc
     1201 aacgtttatt cttgaaagaa gatctctttt agaaatgtat gcagactttt ttgcacatcc
     1261 ggacctgttt gtgagcatta gtgaccagaa ggatcccaag gatcgaatgg ttcaggttgt
     1321 gaaatggtac ctctcagcct ttcatgcggg aaggaaagga tcagttgcca aaaagccata
     1381 caatcccatt ttgggcgaga tttttcagtg tcattggaca ttaccaaatg atactgaaga
     1441 gaacacagaa ctagtttcag aaggaccagt tccctgggtt tccaaaaaca gtgtaacatt
     1501 tgtggctgag caggtttccc atcatccacc catttcagcc ttttatgctg agtgttttaa
     1561 caagaagata caattcaatg ctcatatctg gaccaaatca aaattccttg ggatgtcaat
     1621 tggggtgcac aacatagggc agggctgtgt ctcatgtcta gactatgatg aacattacat
     1681 tctcacattc cccaatggct atggaaggtc tatcctcaca gtgccctggg tggaattagg
     1741 aggagaatgc aatattaatt gttccaaaac aggctatagt gcaaatatca tcttccacac
     1801 taaacccttc tatgggggca agaagcacag aattactgcc gagatttttt ctccaaatga
     1861 caagaagtct ttttgctcaa ttgaagggga atggaatggt gtgatgtatg caaaatatgc
     1921 aacaggggaa aatacagtct ttgtagatac caagaagttg cctataatca agaagaaagt
     1981 gaggaagttg gaagatcaga acgagtatga atcccgcagc ctttggaagg atgtcacttt
     2041 caacttaaaa atcagagaca ttgatgcagc aactgaagca aagcacaggc ttgaagaaag
     2101 acaaagagca gaagcccgag aaaggaagga gaaggaaatt cagtgggaga caaggttatt
     2161 tcatgaagat ggagaatgct gggtttatga tgaaccatta ctgaaacgtc ttggtgctgc
     2221 caagcattag gttggaagat gcaaagttta tacctgatga tcagggcagt aggcataatt
     2281 cagcaacaaa caatcttcct ttgggagaaa cctgttcatt ccaatcttct aattacagtg
     2341 gttcctatct cagggatact ggactttctg acgcagatga acaattaagg ggaaaagctt
     2401 cccttttccc tctgtggcag ttacgatttt gacttcagtc ctgagaaaaa cttcaggttt
     2461 tgaaaatcag atgatgtctt ctccttttcc aaacaccaca cgttgaaagc atttataaat
     2521 ccaagtctga aactctgcgc tctagtactg ctgttaagat acacaacttg tttcttagtt
     2581 catataatct cgggatacac acacacacac acatatatat acacacacat acgtatacac
     2641 acacatacat atatataaat atacctgatg ccagattttt ttcataaata ttctgcctac
     2701 tgtaaatatg ggttcctctg agttgtttta gaaaattagc gcaatgtatt aaaatcaagt
     2761 gttaggaaat ttcatggtct tacctacaat aacttttatt ttggaattga actattatta
     2821 aattgtatct aatcctggat tacagtttaa ttaattattc ttagtgctta aggcttcata
     2881 aagtaatttt tccaaccttt tttttaaaaa tgtgcatctt ttattatctt ttatggttaa
     2941 acttggaagc caattatttg gcaaccttga atacaagagc tagcttttca aagaaagaca
     3001 cttgaaataa tggaggatcc attcttttcc tctgtcagat tgtatgttct gtctttagcc
     3061 cctgccaggc aattccccag aatcttcact taactcatat tgcacatgta gggctgccta
     3121 ccaacatttt atccaaaaat gttttattgc cttagagggt ttgggaagag tgtgtattta
     3181 tagatcccca ccccacagtg aacaaaaata gatagataga tagaataaaa tgaatgccac
     3241 agcagcagat ggctcattct gaagggaaat acagaactat atctatctat ctatctatca
     3301 tcttttttat ttaaaaatac attaaaaaaa caggtattag tacctagcat ttagttagtt
     3361 attcaatata ctggttacgt aaggcctttc aagaagtaga ttctcagtct gagaggacac
     3421 aggccagggc ttctcaaagt gctgctgcag gctttctggt ctcgcttggc ccattctgct
     3481 aattttcctt tgccagctcc tacagtaacc ctgggtttat tagtctcaac aaaggataaa
     3541 aagtaaatca aatgctatga tgccagtgca aaacttcaat ggaagcccta aggcagtagt
     3601 ataactaact ccataaaata caaacaaaca cattttaaaa tacacattgc ttcaggccaa
//
