LOCUS       NM_020841               7204 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens oxysterol binding protein like 8 (OSBPL8), transcript
            variant 1, mRNA.
ACCESSION   NM_020841
VERSION     NM_020841.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Li,J., Cui,J., Li,Z., Fu,X., Li,J., Li,H., Wang,S. and Zhang,M.
  TITLE     ORP8 induces apoptosis by releasing cytochrome c from mitochondria
            in non-small cell lung cancer
  JOURNAL   Oncol Rep 43 (5), 1516-1524 (2020)
   PUBMED   32323800
  REMARK    GeneRIF: ORP8 induces apoptosis by releasing cytochrome c from
            mitochondria in nonsmall cell lung cancer.
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Pulli,I., Lassila,T., Pan,G., Yan,D., Olkkonen,V.M. and
            Tornquist,K.
  TITLE     Oxysterol-binding protein related-proteins (ORPs) 5 and 8 regulate
            calcium signaling at specific cell compartments
  JOURNAL   Cell Calcium 72, 62-69 (2018)
   PUBMED   29748134
  REMARK    GeneRIF: ORP5/8 regulate Ca(2+) signaling at specific membrane
            contact sites foci.
REFERENCE   3  (bases 1 to 7204)
  AUTHORS   Sohn,M., Korzeniowski,M., Zewe,J.P., Wills,R.C., Hammond,G.R.V.,
            Humpolickova,J., Vrzal,L., Chalupska,D., Veverka,V., Fairn,G.D.,
            Boura,E. and Balla,T.
  TITLE     PI(4,5)P2 controls plasma membrane PI4P and PS levels via ORP5/8
            recruitment to ER-PM contact sites
  JOURNAL   J Cell Biol 217 (5), 1797-1813 (2018)
   PUBMED   29472386
  REMARK    GeneRIF: ORP5/8 recruitment to the plasma membrane occurs through
            interactions with the N-terminal Pleckstrin homology domains and
            adjacent basic residues of ORP5/8 with both phosphatidylinositol
            4-phosphate and Phosphatidylinositol 4,5-bisphosphate.
REFERENCE   4  (bases 1 to 7204)
  AUTHORS   Lee,M. and Fairn,G.D.
  TITLE     Both the PH domain and N-terminal region of oxysterol-binding
            protein related protein 8S are required for localization to PM-ER
            contact sites
  JOURNAL   Biochem Biophys Res Commun 496 (4), 1088-1094 (2018)
   PUBMED   29409900
  REMARK    GeneRIF: Examination of a series of deletion constructs
            demonstrated that both the N-terminal polybasic region and the PH
            domain are required for proper targeting of the short splice
            variant ORP8S to the PM-ER contact site in Chinese hamster ovary
            cells.
REFERENCE   5  (bases 1 to 7204)
  AUTHORS   Ghai,R., Du,X., Wang,H., Dong,J., Ferguson,C., Brown,A.J.,
            Parton,R.G., Wu,J.W. and Yang,H.
  TITLE     ORP5 and ORP8 bind phosphatidylinositol-4, 5-biphosphate
            (PtdIns(4,5)P 2) and regulate its level at the plasma membrane
  JOURNAL   Nat Commun 8 (1), 757 (2017)
   PUBMED   28970484
  REMARK    GeneRIF: ORP5/8 are endoplasmic reticulum (ER) membrane proteins
            implicated in lipid trafficking that localize to ER-plasma membrane
            (PM) contacts and maintain membrane homeostasis. Here the authors
            show that PtdIns(4,5)P 2 plays a critical role in the targeting and
            function of ORP5/8 at the PM.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7204)
  AUTHORS   Yan,D., Mayranpaa,M.I., Wong,J., Perttila,J., Lehto,M.,
            Jauhiainen,M., Kovanen,P.T., Ehnholm,C., Brown,A.J. and
            Olkkonen,V.M.
  TITLE     OSBP-related protein 8 (ORP8) suppresses ABCA1 expression and
            cholesterol efflux from macrophages
  JOURNAL   J Biol Chem 283 (1), 332-340 (2008)
   PUBMED   17991739
  REMARK    GeneRIF: Data identify ORP8 as a negative regulator of ABCA1
            expression and macrophage cholesterol efflux. ORP8 may, thus,
            modulate the development of atherosclerosis.
REFERENCE   7  (bases 1 to 7204)
  AUTHORS   Suchanek,M., Hynynen,R., Wohlfahrt,G., Lehto,M., Johansson,M.,
            Saarinen,H., Radzikowska,A., Thiele,C. and Olkkonen,V.M.
  TITLE     The mammalian oxysterol-binding protein-related proteins (ORPs)
            bind 25-hydroxycholesterol in an evolutionarily conserved pocket
  JOURNAL   Biochem J 405 (3), 473-480 (2007)
   PUBMED   17428193
REFERENCE   8  (bases 1 to 7204)
  AUTHORS   Ma,J., Dempsey,A.A., Stamatiou,D., Marshall,K.W. and Liew,C.C.
  TITLE     Identifying leukocyte gene expression patterns associated with
            plasma lipid levels in human subjects
  JOURNAL   Atherosclerosis 191 (1), 63-72 (2007)
   PUBMED   16806233
REFERENCE   9  (bases 1 to 7204)
  AUTHORS   Jaworski,C.J., Moreira,E., Li,A., Lee,R. and Rodriguez,I.R.
  TITLE     A family of 12 human genes containing oxysterol-binding domains
  JOURNAL   Genomics 78 (3), 185-196 (2001)
   PUBMED   11735225
REFERENCE   10 (bases 1 to 7204)
  AUTHORS   Lehto,M., Laitinen,S., Chinetti,G., Johansson,M., Ehnholm,C.,
            Staels,B., Ikonen,E. and Olkkonen,V.M.
  TITLE     The OSBP-related protein family in humans
  JOURNAL   J Lipid Res 42 (8), 1203-1213 (2001)
   PUBMED   11483621
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF392452.1 and AL832876.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020841.4.
            
            Summary: This gene encodes a member of a family of proteins
            containing an N-terminal pleckstrin homology domain and a highly
            conserved C-terminal oxysterol-binding protein-like sterol-binding
            domain. It binds mutliple lipid-containing molecules, including
            phosphatidylserine, phosphatidylinositol 4-phosphate (PI4P) and
            oxysterol, and promotes their exchange between the endoplasmic
            reticulum and the plasma membrane. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB040884.3, AF392452.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261183.8/ ENSP00000261183.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AF392452.1         39-72
            35-7201             AF392452.1         74-7240
            7202-7204           AL832876.1         5294-5296
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q21.2"
     gene            1..7204
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /note="oxysterol binding protein like 8"
                     /db_xref="GeneID:114882"
                     /db_xref="HGNC:HGNC:16396"
                     /db_xref="MIM:606736"
     exon            1..375
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            376..484
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     CDS             443..3112
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /note="isoform a is encoded by transcript variant 1;
                     oxysterol-binding protein-related protein 8; OSBP-related
                     protein 8"
                     /codon_start=1
                     /product="oxysterol-binding protein-related protein 8
                     isoform a"
                     /protein_id="NP_065892.1"
                     /db_xref="CCDS:CCDS31862.1"
                     /db_xref="GeneID:114882"
                     /db_xref="HGNC:HGNC:16396"
                     /db_xref="MIM:606736"
                     /translation="MEGGLADGEPDRTSLLGDSKDVLGPSTVVANSDESQLLTPGKMS
                     QRQGKEAYPTPTKDLHQPSLSPASPHSQGFERGKEDISQNKDESSLSMSKSKSESKLY
                     NGSEKDSSTSSKLTKKESLKVQKKNYREEKKRATKELLSTITDPSVIVMADWLKIRGT
                     LKSWTKLWCVLKPGVLLIYKTQKNGQWVGTVLLNACEIIERPSKKDGFCFKLFHPLEQ
                     SIWAVKGPKGEAVGSITQPLPSSYLIIRATSESDGRCWMDALELALKCSSLLKRTMIR
                     EGKEHDLSVSSDSTHVTFYGLLRANNLHSGDNFQLNDSEIERQHFKDQDMYSDKSDKE
                     NDQEHDESDNEVMGKSEESDTDTSERQDDSYIEPEPVEPLKETTYTEQSHEELGEAGE
                     ASQTETVSEENKSLIWTLLKQVRPGMDLSKVVLPTFILEPRSFLDKLSDYYYHADFLS
                     EAALEENPYFRLKKVVKWYLSGFYKKPKGLKKPYNPILGETFRCLWIHPRTNSKTFYI
                     AEQVSHHPPISAFYVSNRKDGFCLSGSILAKSKFYGNSLSAILEGEARLTFLNRGEDY
                     VMTMPYAHCKGILYGTMTLELGGTVNITCQKTGYSAILEFKLKPFLGSSDCVNQISGK
                     LKLGKEVLATLEGHWDSEVFITDKKTDNSEVFWNPTPDIKQWRLIRHTVKFEEQGDFE
                     SEKLWQRVTRAINAKDQTEATQEKYVLEEAQRQAARDRKTKNEEWSCKLFELDPLTGE
                     WHYKFADTRPWDPLNDMIQFEKDGVIQTKVKHRTPMVSVPKMKHKPTRQQKKVAKGYS
                     SPEPDIQDSSGSEAQSVKPSTRRKKGIELGDIQSSIESIKQTQEEIKRNIMALRNHLV
                     SSTPATDYFLQQKDYFIIFLLILLQVIINFMFK"
     exon            485..521
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            522..659
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            660..730
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            731..808
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            809..910
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1114
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1199
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1371
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1609
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1794
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1795..1876
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..1975
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2072
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            2073..2171
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            2172..2269
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            2270..2359
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2496
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            2497..2682
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            2683..2770
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            2771..2876
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            2877..2979
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            2980..7204
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7181..7186
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /note="hexamer: AATAAA"
     polyA_site      7204
                     /gene="OSBPL8"
                     /gene_synonym="MST120; MSTP120; ORP8; OSBP10"
                     /note="major polyA site"
ORIGIN      
        1 agaaaggcgg cgacgcggcg gatgacaaca ttaggccgcg acgcgctcct ggccaggcgg
       61 cggctgtagt gttagctttg gacgccgcag tagccgctgc cggtagcaag ccgactgagg
      121 gaaggtgggg gtccgcccgg gctggtggac ctcggggccg aaagttcccg ccccgctcgg
      181 gggctgagcc ggcagtgcct ccgcggccgc tgggcagcgc ccttcgtcca ggctcgcgcc
      241 ccagctgccg ccgacgacag cggccgagag aagttggggt ctgactagac gcttacgggg
      301 cctcggaccc cggcgccgcg gcgacctcgg aggaaccggc tccttgcgtc ccgcctccct
      361 gggagctccg cacgggattt gcagatttac agaatggctg cacattaatg gaaagagaag
      421 cataaaccta tcttctttca ttatggaggg aggtttggca gatggagaac ctgatcgaac
      481 ttcgcttctt ggtgatagca aagatgtcct tgggccatca actgttgtag caaacagtga
      541 cgaatctcag cttctgacac caggaaagat gagtcagcgc caaggaaaag aagcttatcc
      601 aacgccaacc aaagatttgc atcagccatc tcttagtcca gcaagtcctc atagccaggg
      661 ttttgaaaga gggaaggaag atatttctca aaataaagat gaatcttcac tttctatgtc
      721 aaagagcaag tctgaatcta aactttataa tggctcagag aaggacagtt caacttcaag
      781 caaactcaca aaaaaagaat ctcttaaggt acaaaagaaa aattaccgag aagaaaagaa
      841 aagagccaca aaggagctgc tcagtacaat cacagatcct tctgttattg ttatggctga
      901 ttggttaaag attcgtggta ctctaaagag ctggaccaag ttatggtgtg tgttgaaacc
      961 tggggtgcta ctgatctata aaacccaaaa aaatggtcag tgggtaggaa cagttcttct
     1021 gaatgcctgt gaaatcattg aacgtccatc aaaaaaggat ggcttttgtt tcaaactttt
     1081 ccatcctttg gagcaatcta tttgggcagt gaagggtcca aaaggtgaag cggttggatc
     1141 cattactcaa cccttaccta gcagttattt gatcatccga gctacttcag agtcagatgg
     1201 aaggtgctgg atggatgctt tggagttggc tttgaaatgt tctagtcttc ttaaacgtac
     1261 aatgatcaga gaaggaaagg aacatgacct gagcgtttca tcagatagca cacatgtgac
     1321 tttctatggc ttactacgtg ctaacaatct ccacagtggt gataacttcc agttaaatga
     1381 tagtgaaatt gaacgacaac attttaagga ccaagatatg tattctgata aatctgataa
     1441 agaaaatgat caagaacatg atgagtctga taatgaggtg atggggaaaa gtgaagaaag
     1501 tgacacagat acatcagaaa gacaagatga ctcatatatc gaacctgagc ctgttgagcc
     1561 tttaaaggag actacctaca ctgaacagag ccatgaagaa cttggagagg caggtgaggc
     1621 ttctcaaaca gaaactgtat ctgaagaaaa caaaagcctt atctggacac tattgaaaca
     1681 agtccgtcct ggcatggacc tatccaaggt ggttctgcct acatttattt tggaaccccg
     1741 ttctttcctg gataaacttt cagattacta ctatcatgca gatttcctat ctgaggcagc
     1801 tcttgaagaa aatccttatt tccgtttgaa gaaagtagtg aaatggtatt tgtcaggatt
     1861 ctataaaaag ccaaagggac tgaagaaacc ttataatcct atacttggcg agactttccg
     1921 ttgtttatgg attcatccca gaacaaacag caaaactttt tatattgctg aacaggtgtc
     1981 ccatcatcca ccaatatctg ccttttatgt tagtaatcga aaagatggat tttgccttag
     2041 cggtagtatc ctggctaagt ctaagtttta tggaaactca ttatctgcaa tattagaggg
     2101 agaagcacgg ttaactttct tgaatagagg tgaagattat gtaatgacaa tgccatacgc
     2161 tcattgtaaa ggaattcttt atggtacaat gacactggag cttggtggaa cagtcaatat
     2221 tacatgtcaa aaaactggat acagtgcaat acttgaattt aaactaaagc cattcctagg
     2281 gagtagtgac tgtgttaatc aaatatcagg gaaacttaaa ctgggaaaag aagtcctagc
     2341 tactttggaa ggtcattggg atagtgaagt ttttattact gataaaaaga ctgataattc
     2401 agaggttttc tggaatccaa cacctgacat taagcaatgg agattaataa ggcacactgt
     2461 aaaatttgaa gaacagggag attttgaatc agagaaactc tggcaacggg taactcgagc
     2521 cataaatgcc aaagaccaaa ctgaagctac ccaagagaag tatgttttgg aagaagctca
     2581 aagacaagct gccagggatc ggaaaacaaa aaatgaagag tggtcttgca aattatttga
     2641 acttgatcca ctcacaggag aatggcatta caagtttgca gatacccgac catgggaccc
     2701 acttaatgat atgatacagt ttgaaaaaga tggtgttatt cagaccaaag tgaaacatcg
     2761 tactccaatg gttagcgtcc ccaaaatgaa acataagcca accaggcaac agaagaaagt
     2821 agcaaaaggc tattcctccc cagaacctga cattcaagac tcctctggaa gtgaagctca
     2881 atcagtaaaa ccaagtacaa gaagaaagaa aggaatagaa ctgggagaca ttcagagttc
     2941 catcgaatct ataaaacaaa cacaggaaga aattaaaaga aatattatgg ctcttcgaaa
     3001 tcatttagtt tcaagcacac cggccacgga ttattttctg caacaaaaag actacttcat
     3061 cattttcctc ctgattttgc ttcaagtcat aataaacttc atgttcaagt agaagttctc
     3121 taccattgaa tcagtgaact agaaagatct gatttggcct gggaccagtg ttcaagttgg
     3181 tttggtcttt attaaaaatc acaatattcc gaaaacaaaa aaacctagga gataaatgta
     3241 gaggtattga cttttcgtat cttttatctt cacactgaaa caagagctat cctatttgat
     3301 tattaaagtg agctatgtgt taagtgccag gacatttcta gcttttgtga gaatgtgtct
     3361 acatatgagt ataataaacc cacatgtata cacaattgtc tcttatgtac tcctacctga
     3421 cagtagtctt tgtattctat agtatgttct gagatataat gttaacattg ttcataacaa
     3481 aaaatgctat caatcttata aatatatgta atctattttc ttcataaaac aggcacaaaa
     3541 gttttatcag taaggaatta cagattgaga aatgatggaa taatagacat aattaattca
     3601 atacactact gttaaaatca tttgcaaagc actcagctca attatcttct tagaaagaaa
     3661 gaaaaagtat gaatggtcaa aatgaataca tcgagagaga taaatggcaa attgcttttt
     3721 taaaagttta cataagtttt ttttaacccc tagaatttaa tatttgtaga tgcaggtaaa
     3781 tatatatact tacgtgtata tcagtataaa aacactggtg tgcaattaat tggattgatt
     3841 ataataccac cttaagcact tgctgaaaaa agtgtggtca aaattgattg ctgtcctttt
     3901 gtcttatttt tgtttttctt aagtcagctg gttcataaca taggccaaat tctagagatg
     3961 tttatagagc atttgaagtg ctgataattt atgttttttc attatgaaaa cttattttag
     4021 ctttagactc cagtgtgttc agtgaataag tagaatataa aaaaatataa ccagtatttt
     4081 acttcaaaag ccaaaaagag gcaataagaa aagacacttt gtggtggcct ttatgtgtgc
     4141 attaaaattg gtttctgtaa aacgtgtaat aagttgagta tctacgaaga gtatcaagtt
     4201 ctgaagttta atttttttat tatcctcctc tcttcttagt aacttctttc tgtggcaaaa
     4261 ccacaattct ttaagattcc tattgttcag gctaaggcaa atttttttgt ttgtttcttc
     4321 agtttaatat tttgattttg tgtttttacg taaatattta tattccttga aagcaatttt
     4381 tgccaaggta gttcagttta ggaatatgtt gttctaaaat atgtcttaga atcctgaaag
     4441 catagatttt gaaatgtttt tttaatgaaa atgaaggtca gagagaataa ttgccctgac
     4501 cacatttgcc tttcagtagg aggaggctgt gaaatagtaa aattataatc gtttatgcca
     4561 tgataaatac aagattggta aataaataca ttgattggta aattatgaga atcaaaatga
     4621 taaaaagagc ctgctttttt ccctaaccaa tatagctatc ttaagtatcc ttaggtttct
     4681 gtgaagaacc atttcccatg ttttcttggc aaaataatgc tgtattccat atgtacatgt
     4741 gaaatgatgt tttaaattga taaaagctta aataagatct acctataccc agtattttca
     4801 tgatattaga acaaatgggt ttttggttat attttatatt tgtcaatata atttttgtat
     4861 tcacattctg ttacactctg cctattcatt gatatatgat attctgtaaa tattgtacaa
     4921 tttgatcttt tttatggttt aaattagtta attacataca aattgattgg cttatcacaa
     4981 aaatcatttc atcagtaaac cttgttaaca ttttgtactg gtgacccacc tcttaggact
     5041 ttggtcttat ccacgtgtat gttgttttca tttggtccaa ataatatttt atttgtatgg
     5101 gtatcttcta agactaaata ggtagttgtg ttctttattt ttaaaatttc tttttagagc
     5161 aaatgttatg ggttcttacc caaagagtca aaaactattt cttaagaaag agcagagtta
     5221 ttcatgactg ttctttatac actaaaagca tgcatctaat ctaatagtcc tcttattatg
     5281 cttttagttg tatgagtctc tttctatgaa ctgaacacaa aactcaggaa ttggtggctt
     5341 aattttagat cagtgcttgt actaggctta gttatatgaa tctttataac acataattac
     5401 taactttgta gccatatatg taattgactt tgaatgttat ttacctgaaa ttaatcttcc
     5461 ttcacacatg gaccgtaaac ggttcccagt tgtctgagag cctcatgagg gtttctagga
     5521 tttatgacct tatgaccagt ttttttcatt taccaagatt ttattttcct acatgaaaat
     5581 ttaattgagt aataattatt cacatgtgca ttttcttttt agctgttaaa tgtactatgc
     5641 catcatccac catttagtaa aatgtagctg gcccaggaca tgtaaaaaaa aaaaaaaaac
     5701 aacaacaata aatagggcat gtgaaatgtt aagttacagc aatagatatt ttatttgtat
     5761 ttcatgttag tacttttttg ttttatatca cttataaagg tacagtgtac tctttgtcac
     5821 agctcagttg gtaaccgcat tccattgaaa agttggcctt gtaaaataca actctcattt
     5881 aatattcatg cttttgtgcc tttaagaaaa tattttttgt cattttttgt gttacagaac
     5941 tataatgtga ttcaaggtgt ttataggctt gtcataaaag ggtcatttct gtgtgttact
     6001 ttctttttat atagctatag tatatttaaa caataatact atcttttata ggggtttgtc
     6061 tatttaccta ttctttactc agacattgat gtagacttgt cagattattc tgagtattgt
     6121 taacagtgcc ttttcgatgg aatcacactt tttggctgtc accttgtgcc atatacacac
     6181 aaaattttgt ggaaggcagt tttaactttc tgaagaatat ctgtcaaaat ttaagaaaac
     6241 aaatgtataa aattccattt tttccagtgt ttagcatttc tagtaagcag tgaggttgtt
     6301 tgacatacag tgatgatggc attattgata agccatacat gagactgcag attatattga
     6361 atcatattaa atgtacagaa ataaaatatt agatttatat caaattttcc aatttgaacc
     6421 agtggggaaa atcccacaga aatcagtaag tttacatttc aatttctatc ttatttgact
     6481 aagtggaaag agattcttta aaatgtataa cctgccatta tgtaatttgg tttcatttta
     6541 ttctacctgt tgtgtgagtt tagtatattt aatttacttt ttgttactct ttacatactg
     6601 tttatttttg ttagttttta attgaagatg gactgttgaa attgtatagg accagtgtct
     6661 tattaatatg attaatatat ttagaagagc cacgtgaaac ccatgacaaa atgaatgtga
     6721 atattctttc taaaaattta gaaaatgtta tctttttgca tttattatgt aaaactgttt
     6781 tacagtatca aaatttttca cttaaagaaa aaaaatgcca tgaaacattt gaactgatga
     6841 gccacagaac ttcagttgaa atttttttca ctttttagca tgctaaatat acatctgagt
     6901 ttaaatgttc tgtttaatgg ccattcataa attcaagcac taccactggt cagttttgtg
     6961 tgatagaata aaaatatgtt acctgcagtg taagtacagc acactgtcaa attcttttcc
     7021 ttaaggtgca cagtaaatgt acagatagtt ataggccact gttttgtaat gtagtacatt
     7081 tctaatctat tattcctaac ctattataac tgtttgcaga aagaaaagaa tttttctaat
     7141 aatctgtaaa attatgctaa cttctacaag taggcttcta aataaaattt ttaaaaagag
     7201 caaa
//
