LOCUS       NM_002553               1924 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens origin recognition complex subunit 5 (ORC5),
            transcript variant 1, mRNA.
ACCESSION   NM_002553
VERSION     NM_002553.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Shibata,E. and Dutta,A.
  TITLE     A human cancer cell line initiates DNA replication normally in the
            absence of ORC5 and ORC2 proteins
  JOURNAL   J Biol Chem 295 (50), 16949-16959 (2020)
   PUBMED   32989049
  REMARK    GeneRIF: A human cancer cell line initiates DNA replication
            normally in the absence of ORC5 and ORC2 proteins.
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Jaremko,M.J., On,K.F., Thomas,D.R., Stillman,B. and Joshua-Tor,L.
  TITLE     The dynamic nature of the human origin recognition complex revealed
            through five cryoEM structures
  JOURNAL   Elife 9, e58622 (2020)
   PUBMED   32808929
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1924)
  AUTHORS   Coulombe,P., Nassar,J., Peiffer,I., Stanojcic,S., Sterkers,Y.,
            Delamarre,A., Bocquet,S. and Mechali,M.
  TITLE     The ORC ubiquitin ligase OBI1 promotes DNA replication origin
            firing
  JOURNAL   Nat Commun 10 (1), 2426 (2019)
   PUBMED   31160578
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1924)
  AUTHORS   Giri,S., Chakraborty,A., Sathyan,K.M., Prasanth,K.V. and
            Prasanth,S.G.
  TITLE     Orc5 induces large-scale chromatin decondensation in a
            GCN5-dependent manner
  JOURNAL   J Cell Sci 129 (2), 417-429 (2016)
   PUBMED   26644179
  REMARK    GeneRIF: Orc5 associates with the H3 histone acetyl transferase
            GCN5 (also known as KAT2A), and this association enhances the
            chromatin-opening function of Orc5.
REFERENCE   5  (bases 1 to 1924)
  AUTHORS   Hein,M.Y., Hubner,N.C., Poser,I., Cox,J., Nagaraj,N., Toyoda,Y.,
            Gak,I.A., Weisswange,I., Mansfeld,J., Buchholz,F., Hyman,A.A. and
            Mann,M.
  TITLE     A human interactome in three quantitative dimensions organized by
            stoichiometries and abundances
  JOURNAL   Cell 163 (3), 712-723 (2015)
   PUBMED   26496610
REFERENCE   6  (bases 1 to 1924)
  AUTHORS   Thome,K.C., Dhar,S.K., Quintana,D.G., Delmolino,L., Shahsafaei,A.
            and Dutta,A.
  TITLE     Subsets of human origin recognition complex (ORC) subunits are
            expressed in non-proliferating cells and associate with non-ORC
            proteins
  JOURNAL   J Biol Chem 275 (45), 35233-35241 (2000)
   PUBMED   10954718
REFERENCE   7  (bases 1 to 1924)
  AUTHORS   Tugal,T., Zou-Yang,X.H., Gavin,K., Pappin,D., Canas,B.,
            Kobayashi,R., Hunt,T. and Stillman,B.
  TITLE     The Orc4p and Orc5p subunits of the Xenopus and human origin
            recognition complex are related to Orc1p and Cdc6p
  JOURNAL   J Biol Chem 273 (49), 32421-32429 (1998)
   PUBMED   9829972
REFERENCE   8  (bases 1 to 1924)
  AUTHORS   Quintana,D.G., Thome,K.C., Hou,Z.H., Ligon,A.H., Morton,C.C. and
            Dutta,A.
  TITLE     ORC5L, a new member of the human origin recognition complex, is
            deleted in uterine leiomyomas and malignant myeloid diseases
  JOURNAL   J Biol Chem 273 (42), 27137-27145 (1998)
   PUBMED   9765232
REFERENCE   9  (bases 1 to 1924)
  AUTHORS   Ishiai,M., Dean,F.B., Okumura,K., Abe,M., Moon,K.Y., Amin,A.A.,
            Kagotani,K., Taguchi,H., Murakami,Y., Hanaoka,F., O'Donnell,M.,
            Hurwitz,J. and Eki,T.
  TITLE     Isolation of human and fission yeast homologues of the budding
            yeast origin recognition complex subunit ORC5: human homologue
            (ORC5L) maps to 7q22
  JOURNAL   Genomics 46 (2), 294-298 (1997)
   PUBMED   9417919
REFERENCE   10 (bases 1 to 1924)
  AUTHORS   Klemm,R.D., Austin,R.J. and Bell,S.P.
  TITLE     Coordinate binding of ATP and origin DNA regulates the ATPase
            activity of the origin recognition complex
  JOURNAL   Cell 88 (4), 493-502 (1997)
   PUBMED   9038340
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK223361.1, BC023652.2 and
            AC007393.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002553.3.
            
            Summary: The origin recognition complex (ORC) is a highly conserved
            six subunit protein complex essential for the initiation of the DNA
            replication in eukaryotic cells. Studies in yeast demonstrated that
            ORC binds specifically to origins of replication and serves as a
            platform for the assembly of additional initiation factors such as
            Cdc6 and Mcm proteins. The protein encoded by this gene is a
            subunit of the ORC complex. Alternatively spliced transcript
            variants encoding distinct isoforms have been described. [provided
            by RefSeq, Oct 2010].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK223361.1, SRR3476690.1144498.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000297431.9/ ENSP00000297431.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                AK223361.1         35-62
            29-48               BC023652.2         1-20
            49-49               AC007393.3         58070-58070         c
            50-1371             BC023652.2         22-1343
            1372-1924           AK223361.1         1406-1958
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1-q22.2"
     gene            1..1924
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /note="origin recognition complex subunit 5"
                     /db_xref="GeneID:5001"
                     /db_xref="HGNC:HGNC:8491"
                     /db_xref="MIM:602331"
     exon            1..181
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..1417
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein phosphatase 1, regulatory subunit 117"
                     /codon_start=1
                     /product="origin recognition complex subunit 5 isoform 1"
                     /protein_id="NP_002544.1"
                     /db_xref="CCDS:CCDS5734.1"
                     /db_xref="GeneID:5001"
                     /db_xref="HGNC:HGNC:8491"
                     /db_xref="MIM:602331"
                     /translation="MPHLENVVLCRESQVSILQSLFGERHHFSFPSIFIYGHTASGKT
                     YVTQTLLKTLELPHVFVNCVECFTLRLLLEQILNKLNHLSSSEDGCSTEITCETFNDF
                     VRLFKQVTTAENLKDQTVYIVLDKAEYLRDMEANLLPGFLRLQELADRNVTVLFLSEI
                     VWEKFRPNTGCFEPFVLYFPDYSIGNLQKILSHDHPPEYSADFYAAYINILLGVFYTV
                     CRDLKELRHLAVLNFPKYCEPVVKGEASERDTRKLWRNIEPHLKKAMQTVYLREISSS
                     QWEKLQKDDTDPGQLKGLSAHTHVELPYYSKFILIAAYLASYNPARTDKRFFLKHHGK
                     IKKTNFLKKHEKTSNHLLGPKPFPLDRLLAILYSIVDSRVAPTANIFSQITSLVTLQL
                     LTLVGHDDQLDGPKYKCTVSLDFIRAIARTVNFDIIKYLYDFL"
     exon            182..274
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            275..475
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            476..550
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            551..662
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            663..793
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            794..842
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            843..933
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            934..986
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1099
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1147
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1258
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1371
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1924
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1900..1905
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /note="hexamer: AATACA"
     polyA_site      1924
                     /gene="ORC5"
                     /gene_synonym="ORC5L; ORC5P; ORC5T; PPP1R117"
                     /note="major polyA site"
ORIGIN      
        1 gggtccggtt tccggcggga gcgtgggccg ccagactcgg gagaggctcc gtcttgtgca
       61 agggtcctgt gggctggctg cactggcctc tgcggtggtg cctgccagaa tgccccactt
      121 ggaaaacgtg gtgctttgtc gcgagtctca agtgtccatc ttgcagtcct tgtttggaga
      181 gagacatcat ttcagctttc catccatttt tatttatgga catactgcta gtggaaagac
      241 ctatgtaaca caaacgttgt tgaaaacttt agagctccca catgtgtttg tgaattgtgt
      301 tgaatgcttt acattgaggc tgcttttgga acaaatttta aacaaattga atcatcttag
      361 ttcttcagag gatggatgtt ctactgaaat aacctgtgaa acatttaatg actttgttcg
      421 cttgtttaaa caagtaacca cagctgaaaa tcttaaagat cagactgtat atattgttct
      481 agataaagca gagtatctaa gagatatgga agcaaatctt ttgcctggat ttcttagatt
      541 acaagaattg gctgacagaa atgtgactgt tctctttctc agtgaaattg tttgggaaaa
      601 gtttcgtcca aatactggat gctttgagcc gtttgtctta tatttccctg attacagcat
      661 aggcaacctt caaaagatcc tgtcccatga tcatcctcca gagtattcag ctgatttcta
      721 tgctgcctac attaacattc ttcttggagt tttctacact gtttgtcgag atttgaaaga
      781 gctcagacat ctggcagtac ttaattttcc taaatattgt gaacccgtgg ttaaaggaga
      841 agcaagtgaa cgtgatactc gcaaactgtg gagaaatatt gaacctcatt tgaagaaagc
      901 tatgcagact gtttatctca gggaaatatc aagttcccag tgggaaaagc tacagaaaga
      961 tgacacagat ccggggcaac tgaaaggcct ctcagcgcat actcatgtgg aacttccata
     1021 ttactctaag ttcattctaa ttgctgcata ccttgcttca tacaatccag caagaactga
     1081 caagaggttt tttcttaagc atcatggaaa aatcaagaaa accaactttc taaaaaaaca
     1141 cgaaaagaca agcaatcatc tccttgggcc aaaaccattt ccactagaca gattattagc
     1201 aatattatat agtatcgtgg acagcagagt tgctccaaca gcaaatattt tttcccagat
     1261 tacctctcta gtgacccttc agctgttaac cctggttggc catgacgatc agcttgatgg
     1321 accaaaatac aaatgcacag tgtctctaga cttcatcaga gctattgcaa ggacggtgaa
     1381 ctttgacata ataaaatact tgtatgattt cttgtgaaaa caagcttcaa agccatatgg
     1441 acactgtgac aatgactaag ccaagctgtg ttcatccagc tacttagctg gccaaggaga
     1501 ggagttcttt ggctctattg gatttgtcca aacaggtgct ggcccagcat ggaatctgat
     1561 gaaaatattc tgattggtct gggtggatgt gagcagaaga ctatttacca gggaccctgg
     1621 agtatttgga agcaacgtgt taattataaa cagcagggtt tgagcacaat ctgttctact
     1681 cttaatgatg ttatcttaac actgaaattg cctgaaaccc atttacttag gactacattt
     1741 tgctctgtga actatcccct gcgctttgaa cgtgccagca gcccttgttt atatgcccat
     1801 tcttttcact tcctctccac aggagcctct gcagtcgctt gccaaagcag attttcctaa
     1861 ggccactgtt ttaaaagatc atagttgcaa aatataataa atacaagttc tttttaaaat
     1921 ccaa
//
