LOCUS       NM_181741               6547 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens origin recognition complex subunit 4 (ORC4),
            transcript variant 1, mRNA.
ACCESSION   NM_181741
VERSION     NM_181741.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Jaremko,M.J., On,K.F., Thomas,D.R., Stillman,B. and Joshua-Tor,L.
  TITLE     The dynamic nature of the human origin recognition complex revealed
            through five cryoEM structures
  JOURNAL   Elife 9, e58622 (2020)
   PUBMED   32808929
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6547)
  AUTHORS   Choi,S.G., Olivet,J., Cassonnet,P., Vidalain,P.O., Luck,K.,
            Lambourne,L., Spirohn,K., Lemmens,I., Dos Santos,M., Demeret,C.,
            Jones,L., Rangarajan,S., Bian,W., Coutant,E.P., Janin,Y.L., van der
            Werf,S., Trepte,P., Wanker,E.E., De Las Rivas,J., Tavernier,J.,
            Twizere,J.C., Hao,T., Hill,D.E., Vidal,M., Calderwood,M.A. and
            Jacob,Y.
  TITLE     Maximizing binary interactome mapping with a minimal number of
            assays
  JOURNAL   Nat Commun 10 (1), 3907 (2019)
   PUBMED   31467278
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6547)
  AUTHORS   Sanchez,J.C., Kwan,E.X., Pohl,T.J., Amemiya,H.M., Raghuraman,M.K.
            and Brewer,B.J.
  TITLE     Defective replication initiation results in locus specific
            chromosome breakage and a ribosomal RNA deficiency in yeast
  JOURNAL   PLoS Genet 13 (10), e1007041 (2017)
   PUBMED   29036220
  REMARK    GeneRIF: Since the site of one of the missense mutations in the
            human ORC4 alleles is conserved between humans and yeast, we sought
            to determine in what way this single amino acid change affects the
            process of chromosome replication, by introducing the comparable
            mutation into yeast (orc4Y232C).
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6547)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 6547)
  AUTHORS   Springer,J., Kneissl,M., Putter,V. and Grummt,F.
  TITLE     Identification and characterization of MmORC4 and MmORC5, two
            subunits of the mouse origin of replication recognition complex
  JOURNAL   Chromosoma 108 (4), 243-249 (1999)
   PUBMED   10460412
  REMARK    GeneRIF: Biochemical characterization of the mouse homolog
REFERENCE   7  (bases 1 to 6547)
  AUTHORS   Herbig,U., Marlar,C.A. and Fanning,E.
  TITLE     The Cdc6 nucleotide-binding site regulates its activity in DNA
            replication in human cells
  JOURNAL   Mol Biol Cell 10 (8), 2631-2645 (1999)
   PUBMED   10436018
REFERENCE   8  (bases 1 to 6547)
  AUTHORS   Tugal,T., Zou-Yang,X.H., Gavin,K., Pappin,D., Canas,B.,
            Kobayashi,R., Hunt,T. and Stillman,B.
  TITLE     The Orc4p and Orc5p subunits of the Xenopus and human origin
            recognition complex are related to Orc1p and Cdc6p
  JOURNAL   J Biol Chem 273 (49), 32421-32429 (1998)
   PUBMED   9829972
REFERENCE   9  (bases 1 to 6547)
  AUTHORS   Eki,T., Dean,F.B., Kohda,A., Okumura,K., Abe,M., Murakami,Y.,
            Ishiai,M., Satomoto,K., Hurwitz,J., O'Donnell,M. and Hanaoka,F.
  TITLE     Assignment of the homologue of the yeast origin recognition complex
            subunit ORC4 (ORC4L) to human chromosome band 2q22-->q23 by in situ
            hybridization and somatic cell hybrid analysis
  JOURNAL   Cytogenet Cell Genet 81 (1), 89-90 (1998)
   PUBMED   9691185
REFERENCE   10 (bases 1 to 6547)
  AUTHORS   Quintana,D.G., Hou,Zh., Thome,K.C., Hendricks,M., Saha,P. and
            Dutta,A.
  TITLE     Identification of HsORC4, a member of the human origin of
            replication recognition complex
  JOURNAL   J Biol Chem 272 (45), 28247-28251 (1997)
   PUBMED   9353276
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA227202.1, BC014847.1 and
            AC009480.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_181741.3.
            
            Summary: The origin recognition complex (ORC) is a highly conserved
            six subunit protein complex essential for the initiation of the DNA
            replication in eukaryotic cells. Studies in yeast demonstrated that
            ORC binds specifically to origins of replication and serves as a
            platform for the assembly of additional initiation factors such as
            Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC
            complex. Several alternatively spliced transcript variants, some of
            which encode the same protein, have been reported for this gene.
            [provided by RefSeq, Oct 2010].
            
            Transcript Variant: This variant (1) lacks an exon in the 5' UTR,
            compared to variant 4. Variants 1 through 4 encode the same protein
            (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.198057.1,
                                           SRR1660809.113610.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392857.10/ ENSP00000376597.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-285               DA227202.1         158-442
            286-1680            BC014847.1         277-1671
            1681-6547           AC009480.4         19832-24698
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q23.1"
     gene            1..6547
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /note="origin recognition complex subunit 4"
                     /db_xref="GeneID:5000"
                     /db_xref="HGNC:HGNC:8490"
                     /db_xref="MIM:603056"
     exon            1..105
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            106..179
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     CDS             123..1433
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /note="isoform 1 is encoded by transcript variant 1;
                     origin recognition complex, subunit 4 homolog"
                     /codon_start=1
                     /product="origin recognition complex subunit 4 isoform 1"
                     /protein_id="NP_859525.1"
                     /db_xref="CCDS:CCDS2187.1"
                     /db_xref="GeneID:5000"
                     /db_xref="HGNC:HGNC:8490"
                     /db_xref="MIM:603056"
                     /translation="MSSRKSKSNSLIHTECLSQVQRILRERFCRQSPHSNLFGVQVQY
                     KHLSELLKRTALHGESNSVLIIGPRGSGKTMLINHALKELMEIEEVSENVLQVHLNGL
                     LQINDKIALKEITRQLNLENVVGDKVFGSFAENLSFLLEALKKGDRTSSCPVIFILDE
                     FDLFAHHKNQTLLYNLFDISQSAQTPIAVIGLTCRLDILELLEKRVKSRFSHRQIHLM
                     NSFGFPQYVKIFKEQLSLPAEFPDKVFAEKWNENVQYLSEDRSVQEVLQKHFNISKNL
                     RSLHMLLMLALNRVTASHPFMTAVDLMEASQLCSMDSKANIVHGLSVLEICLIIAMKH
                     LNDIYEEEPFNFQMVYNEFQKFVQRKAHSVYNFEKPVVMKAFEHLQQLELIKPMERTS
                     GNSQREYQLMKLLLDNTQIMNALQKYPNCPTDVRQWATSSLSWL"
     exon            180..256
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            257..347
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            348..423
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            424..509
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            510..558
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            559..710
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            711..884
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            885..971
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1080
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1176
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1244
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..6547
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1657..1662
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /note="hexamer: AATAAA"
     polyA_site      1680
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /note="major polyA site"
     regulatory      2520..2525
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /note="hexamer: AGTAAA"
     polyA_site      2546
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
     regulatory      2959..2964
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /note="hexamer: AATAAA"
     polyA_site      2984
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
     regulatory      4020..4025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /note="hexamer: AATGAA"
     polyA_site      4056
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
     regulatory      6525..6530
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
                     /note="hexamer: TATAAA"
     polyA_site      6547
                     /gene="ORC4"
                     /gene_synonym="ORC4L; ORC4P"
ORIGIN      
        1 agtggttgtg tacggtccgc agcggcaggt gaagtctagc agaggacgcg gccaggcgat
       61 tcggtgaagc gattcctgca ggcgttggtt cccctctttg acctggattt gaatttgttg
      121 aaatgagcag tcgtaaatca aagagtaaca gcttaattca cacagagtgc ctttcacagg
      181 tacaaagaat tttacgtgaa agattttgtc gtcagagtcc acatagtaac ctatttggag
      241 tgcaagtaca atacaaacac ttaagtgagc tgctgaaaag aactgctctc catggagaga
      301 gtaactctgt ccttattatc ggaccccgag gatcaggaaa aactatgtta ataaatcatg
      361 ctttgaaaga actcatggaa atagaagaag tgagtgaaaa tgtattacaa gttcacttaa
      421 atggactgct gcagatcaat gacaaaatcg ccctaaagga aatcacaagg cagttaaatc
      481 tggaaaatgt agttggagat aaagtttttg gaagctttgc tgaaaacctt tcatttcttc
      541 tggaagcttt aaaaaaaggt gaccgaacta gcagttgccc agtgatcttc atattagatg
      601 aatttgatct ttttgctcat cataaaaacc aaacacttct ctataatctt tttgacattt
      661 ctcagtctgc acagacccca atagcagtta ttggtcttac atgtagattg gatattttgg
      721 aactcttaga aaaaagagtg aagtcaagat tttctcaccg gcagatacac ttaatgaatt
      781 catttggttt tccacagtat gttaaaatat ttaaagaaca gttatctcta cctgcagagt
      841 ttccagacaa ggtttttgct gagaagtgga atgaaaatgt tcagtatctc tcagaagata
      901 gaagtgtgca agaagtacta cagaagcatt tcaatatcag caaaaacctg cggtcattac
      961 acatgctatt gatgcttgct ttaaatcgag taacagcatc gcacccattt atgactgccg
     1021 tagatctaat ggaagcaagc caactgtgta gcatggactc gaaagcaaat attgtacatg
     1081 gtctatcagt cttggaaatc tgtcttataa tagcaatgaa acatttaaat gacatctatg
     1141 aggaagagcc atttaatttt caaatggtct ataatgagtt tcagaagttt gttcaaagga
     1201 aagcacattc cgtttataat tttgaaaaac ctgttgtcat gaaggctttt gaacacttgc
     1261 agcaattaga attaataaag cccatggaaa gaacttcagg aaattcacag agagagtacc
     1321 agctgatgaa actgcttttg gataatactc aaattatgaa tgctctgcag aaatatccca
     1381 actgtcctac agatgtgagg cagtgggcaa catcctcact aagctggtta tgaatataac
     1441 cagtgacttc aactttggca tttcattcat acttctgtag agaacggaaa actattgtcc
     1501 attaacatga tatgctaaac attctataaa cattcttgta tttatgtgag acttgcccat
     1561 ctactgtctt ggctgtgtct tgccttttaa tcatgaacag ttacatgatt tataatttca
     1621 ctgattgaga ttactttgta agtagctgtt cagaagaata aaatatgact gttttaggga
     1681 ctagaccatg tgctttttta acacttatat atataatggt ctatttgaag agctcacttc
     1741 aacctaacag ctagatgtct ttacaaacct taaaccaaag gagtaaaaaa aacaatggta
     1801 agcactgaag tataataagt aacctttggt acagcaggtt tgctgcagtg tttttttctg
     1861 tccacatgca aattttggat tctatcccag acccaggttt tctagttcag aagactaacc
     1921 agcttagtca gaagatggtt ccatggaaga aaaaggccaa ggagtttgaa gatttttctt
     1981 ctagacatct caaaatgtgg tactcatacc atccacatca gaatcccttg taggattttc
     2041 taaaattaca gattgttgag cctaccatag gtcaaaagga ctggaatttt ccttcttaac
     2101 aagtatagtc atggcaccac gtaacatttt ggtcaatgac attgtataaa gggtggtctc
     2161 ataagattat accatatttt tactgtacct tttctatgtc taaatataca aatgttttac
     2221 cattgtgtta cagttgccta cagtatgatt acagtaacat tctgtacagt gctatagctg
     2281 aagagtaata ggctaggcta tctgtatagc ctaagtgtgt aacaggctat atacatatca
     2341 tcagggtctg tgtaagtata ctctgtgatg ttcatacagt gatgaaatca cccaaagaca
     2401 catttctcag aacgtagccc catcatgaag tgacgtgact accagagatt tctggaaaag
     2461 cactcccaaa agtaatgaga tgtgtatggc aatgttaact gaacctgttt gtattaaaaa
     2521 gtaaatccct atgaggaatg tggaaatgtc cgtcaaagca ttgtctttta agtaagttcc
     2581 aagatccatg aattaacaga ggcatatatt taaaatgaaa tttaatgagc acttagtgac
     2641 ttcatttgtt caagaatatt tgatttccta ttgtgtgcat gggaggttta caaaagacag
     2701 actgtcccta tgcttgtgca gcttacaatc tatttaacta ttagaggccc tgtgagatgt
     2761 aggcagttat ttacagatga gaaaactaac acagcagtgg caatgctggg aatggccaac
     2821 aggcttggaa ttcaactcag tttagcacca aagccagtgc tattaagacc tgaagttcaa
     2881 cagtggattc cttaccaaat agtttttaaa gtatgctcta aggaacaatg ttttaatgaa
     2941 atcttggata caaagtggaa taaaaggttt ctgagcataa agtagctctt cagtagttac
     3001 aacctattct tttgcataca tagcctttcc ttccctgcaa aaagttaagc ctgctaagta
     3061 ttcagatccc tgtcatttac ttctcagcag tctcattcaa gctaaggctt gcatactcaa
     3121 tctgtgccgt agtctacaca tttttagggg atatttcacc aaaatacttg acatggtaac
     3181 atcactatta tatactagaa gcaagaattt tacacttatg ctctaagtga tccttttaaa
     3241 aattttctga actatttgag gaaagtatta tttgtcttaa agggtaacag cctcatgaaa
     3301 tgacttgagg attccaattt cttattagct taagatgtca cttaaaagct gacagccctc
     3361 atctgtctca ttggctacat aagagctgca gaagatccat caataaccaa gtataaagaa
     3421 agattttaag actaagtacc aattgaggta aacatgaaac cgttataaag caaattaaaa
     3481 aaaaatacat taggaaaagt tacacaaatg aagtgtacgc ctattatatt cccaaggcat
     3541 ttttgtgggg aggaacagtg ctgcttgttg gcctttccat attccctcat acaaaaatgc
     3601 taaaatctat tcgcaagagt tttgagctaa cttcgtctct taaattgctt taattgtcat
     3661 caataaaaac aaatatgaaa aaccctagtt taaaaagcgg ttcaagaata atgtaaactt
     3721 atacatatag cttattttct tcatttacta gaggaggaaa aatagaacca aaaaatgcat
     3781 gaactcctgc ttttcaatct gattacctca aactgaaaag caggtgaaag gaggaatatt
     3841 tcagtgtgtt tttacatctg aatccaaggg atccaagtgg cccagcagaa acggaattct
     3901 tgttaaaatg actgattctc gtgcatgtga aaggctctgg ggaatgaata cctgtttggt
     3961 ccaaacttac tgtatcataa tgctgtatat gttgaaaacg taatacctgt taatgtccta
     4021 atgaatcacg ttttgcagaa aacaccgtaa gaaacacttc agtctttctg tagtttccat
     4081 gcatatttca ccatggttcg cactctgatc ctcatctggt ttagaaagca attcagtttc
     4141 tttcaaacca ccataaccta agtgctgccc catccttcac ctttacccta gaaaatctga
     4201 gaatccattg gtgtgtactg tgtgacacaa tgccaacaag cctctggttt ctaatactgg
     4261 caggaccaac acacatactt tagtgggaat ataatctgtt tagacgataa tagagggaaa
     4321 tgccataatt aacaaatctc ccttcactca ccactatcct cattcttgca gttgtcaagg
     4381 tgtaagcctc actgtaagtt acaaagctac cactcccctc tttaagaaca catagcttag
     4441 gattgccttg gcgatgtggg ctcttttttg gttccatatg aacttaagta gttttttcca
     4501 attctgtgaa gaaagtcatt ggtagcttga tggggatggc attgaatctg taaattacct
     4561 tgggcagtat ggccattttc acaatattga ttcttcctac ccaagagcat ggaatgttct
     4621 tccatttgtt tgtatcctct tttatttcct tgagcagtag cttgtagttc tccttgaaga
     4681 ggtccttcac atcccttgta agttggattc ctaggtattt tattctcttt gaagcaattg
     4741 tgaatgggag ttcactcatg atttggctct ctgtttgtct gttgttggtg tataagaatg
     4801 cttgtgattt ttgtacattg attttgtatc ctgagacttt gctgaagttg cttatcagct
     4861 taaggagatt ttgggctgag acgatggggt tttctagata tacaatcatg tcgtctgcaa
     4921 acggacaatt tgacttcctc ttttcctaat tgaataccct ttatttcctt ctcctgccta
     4981 attgccctgg ccagaacttc caacactatg ttgaatacga gtggtgagag agggcatccc
     5041 tgtcttgtgc cagttttcaa agggaatgct tccagttttt gcccattcag tatgatattg
     5101 gctgtgggtt tgtcatagat agctcttatt attttgaaat acaccccatc aatccctaat
     5161 ttattgagag tttttagcat gaagggttgt tgaattttgt caaaggcttt ttctgcatct
     5221 attgagataa tcatgtgttt tttgtctttg gctctgttta tatgctggat tacattcatt
     5281 gatttgcgta tattgaacca gccttgcatc ccagggatga agcccacttg atcatggtgg
     5341 ataagctttt tgatgtgctg ctggattcgt tttgccagta ttttatccag ttagaatggc
     5401 aatcattaaa aagtcaggaa acaacaggtg ctggagagga tgtggagaaa taggaacact
     5461 ttcacactgt tggtgggact gtaaactagt tcaaccattg tggaagtcag tgtggcgatt
     5521 cctcagggat ctagaactgg aaacaccatt tgacccagcc atcccattac tgggtatata
     5581 cccaaaggac tataaatcat gctgctataa agacacatgc acacgtatgt ttattgtggc
     5641 attattcaca atagcaaaga cttggaacca acccaaatgt ccaacaatga tagactggat
     5701 taagaaaacg tggcacatat acaccatgga atactatgca gccataaaaa atgatgagtt
     5761 catgtccttt gtagggacat ggatgaaatt ggaaatcatc attctcagta aactatcaca
     5821 agaacaaaaa accaaacacc gcatattctc actcataggt gggaattgaa caatgagatc
     5881 acatggacac aggaagggga atatcacact ctggggactg ttgtggggtg gggggatggg
     5941 ggagggatag cattaggaga tatacctaat gctagatgac gagttagtgg gtgcagcgca
     6001 ccagcatggc acatgtatac ctatgtaact aacctgcaca atgtgcacat gtaccctaaa
     6061 acttaaagta taaaaaaaaa aaaaaacata gctaaagaaa aactgcatat gcaaatcata
     6121 ccgtattcat ttttttattt aacttgggga tggaatgggg atagcataca ttttatttgt
     6181 acaatattta acaatcactt atccaaggtg gggttttatg gggattttaa gctttctgtt
     6241 aaatacttgc agaaaaaata gcaagtacga tttgacagta gtacaataac ctgtgcccaa
     6301 aacacaagaa atacagagta aagcgaaaat aactgcttac caatattctg agaggtaaca
     6361 aatgggatat gggttgagac tgcataaaaa acgtactgct ggttgaatat ctgaggtaaa
     6421 tgatgttcaa tcaagatgct acttgtatca ttttgcccat aaatggataa ataattttat
     6481 gcatatcata ccaaatgttc ctaaaaagta atctttcaaa aaagtataaa agagaaactg
     6541 gaaagta
//
