LOCUS       NM_012381               2498 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens origin recognition complex subunit 3 (ORC3),
            transcript variant 2, mRNA.
ACCESSION   NM_012381
VERSION     NM_012381.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Jaremko,M.J., On,K.F., Thomas,D.R., Stillman,B. and Joshua-Tor,L.
  TITLE     The dynamic nature of the human origin recognition complex revealed
            through five cryoEM structures
  JOURNAL   Elife 9, e58622 (2020)
   PUBMED   32808929
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Coulombe,P., Nassar,J., Peiffer,I., Stanojcic,S., Sterkers,Y.,
            Delamarre,A., Bocquet,S. and Mechali,M.
  TITLE     The ORC ubiquitin ligase OBI1 promotes DNA replication origin
            firing
  JOURNAL   Nat Commun 10 (1), 2426 (2019)
   PUBMED   31160578
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2498)
  AUTHORS   Kuo,A.J., Song,J., Cheung,P., Ishibe-Murakami,S., Yamazoe,S.,
            Chen,J.K., Patel,D.J. and Gozani,O.
  TITLE     The BAH domain of ORC1 links H4K20me2 to DNA replication licensing
            and Meier-Gorlin syndrome
  JOURNAL   Nature 484 (7392), 115-119 (2012)
   PUBMED   22398447
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2498)
  AUTHORS   Comuzzie,A.G., Cole,S.A., Laston,S.L., Voruganti,V.S., Haack,K.,
            Gibbs,R.A. and Butte,N.F.
  TITLE     Novel genetic loci identified for the pathophysiology of childhood
            obesity in the Hispanic population
  JOURNAL   PLoS One 7 (12), e51954 (2012)
   PUBMED   23251661
REFERENCE   5  (bases 1 to 2498)
  AUTHORS   Bartke,T., Vermeulen,M., Xhemalce,B., Robson,S.C., Mann,M. and
            Kouzarides,T.
  TITLE     Nucleosome-interacting proteins regulated by DNA and histone
            methylation
  JOURNAL   Cell 143 (3), 470-484 (2010)
   PUBMED   21029866
REFERENCE   6  (bases 1 to 2498)
  AUTHORS   Izumi,M., Yanagi,K., Mizuno,T., Yokoi,M., Kawasaki,Y., Moon,K.Y.,
            Hurwitz,J., Yatagai,F. and Hanaoka,F.
  TITLE     The human homolog of Saccharomyces cerevisiae Mcm10 interacts with
            replication factors and dissociates from nuclease-resistant nuclear
            structures in G(2) phase
  JOURNAL   Nucleic Acids Res 28 (23), 4769-4777 (2000)
   PUBMED   11095689
REFERENCE   7  (bases 1 to 2498)
  AUTHORS   Thome,K.C., Dhar,S.K., Quintana,D.G., Delmolino,L., Shahsafaei,A.
            and Dutta,A.
  TITLE     Subsets of human origin recognition complex (ORC) subunits are
            expressed in non-proliferating cells and associate with non-ORC
            proteins
  JOURNAL   J Biol Chem 275 (45), 35233-35241 (2000)
   PUBMED   10954718
REFERENCE   8  (bases 1 to 2498)
  AUTHORS   Herbig,U., Marlar,C.A. and Fanning,E.
  TITLE     The Cdc6 nucleotide-binding site regulates its activity in DNA
            replication in human cells
  JOURNAL   Mol Biol Cell 10 (8), 2631-2645 (1999)
   PUBMED   10436018
REFERENCE   9  (bases 1 to 2498)
  AUTHORS   Pinto,S., Quintana,D.G., Smith,P., Mihalek,R.M., Hou,Z.H.,
            Boynton,S., Jones,C.J., Hendricks,M., Velinzon,K.,
            Wohlschlegel,J.A., Austin,R.J., Lane,W.S., Tully,T. and Dutta,A.
  TITLE     latheo encodes a subunit of the origin recognition complex and
            disrupts neuronal proliferation and adult olfactory memory when
            mutant
  JOURNAL   Neuron 23 (1), 45-54 (1999)
   PUBMED   10402192
  REMARK    GeneRIF: Functional studies of the Drosophila homolog
REFERENCE   10 (bases 1 to 2498)
  AUTHORS   Tugal,T., Zou-Yang,X.H., Gavin,K., Pappin,D., Canas,B.,
            Kobayashi,R., Hunt,T. and Stillman,B.
  TITLE     The Orc4p and Orc5p subunits of the Xenopus and human origin
            recognition complex are related to Orc1p and Cdc6p
  JOURNAL   J Biol Chem 273 (49), 32421-32429 (1998)
   PUBMED   9829972
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF125507.1 and BM971022.1.
            
            On Feb 27, 2020 this sequence version replaced NM_012381.3.
            
            Summary: The origin recognition complex (ORC) is a highly conserved
            six subunits protein complex essential for the initiation of the
            DNA replication in eukaryotic cells. Studies in yeast demonstrated
            that ORC binds specifically to origins of replication and serves as
            a platform for the assembly of additional initiation factors such
            as Cdc6 and Mcm proteins. The protein encoded by this gene is a
            subunit of the ORC complex. Studies of a similar gene in Drosophila
            suggested a possible role of this protein in neuronal proliferation
            and olfactory memory. Alternatively spliced transcript variants
            encoding distinct isoforms have been reported for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) lacks three nts in an
            alternate splicing site in the 3' coding, as compared to variant 1.
            The translation reading frame remains the same, and the resulting
            isoform (2) lacks an internal aa, as compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC035494.2, AF093535.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392844.8/ ENSP00000376586.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2495              AF125507.1         2-2496
            2496-2498           BM971022.1         21-23               c
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q15"
     gene            1..2498
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /note="origin recognition complex subunit 3"
                     /db_xref="GeneID:23595"
                     /db_xref="HGNC:HGNC:8489"
                     /db_xref="MIM:604972"
     exon            1..58
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..2170
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /note="isoform 2 is encoded by transcript variant 2;
                     origin recognition complex, subunit 3 honolog; homolog of
                     latheo, Drosophila; origin recognition complex subunit
                     Latheo"
                     /codon_start=1
                     /product="origin recognition complex subunit 3 isoform 2"
                     /protein_id="NP_036513.2"
                     /db_xref="CCDS:CCDS43486.1"
                     /db_xref="GeneID:23595"
                     /db_xref="HGNC:HGNC:8489"
                     /db_xref="MIM:604972"
                     /translation="MATSSMSKGCFVFKPNSKKRKISLPIEDYFNKGKNEPEDSKLRF
                     ETYQLIWQQMKSENERLQEELNKNLFDNLIEFLQKSHSGFQKNSRDLGGQIKLREIPT
                     AALVLGVNVTDHDLTFGSLTEALQNNVTPYVVSLQAKDCPDMKHFLQKLISQLMDCCV
                     DIKSKEEESVHVTQRKTHYSMDSLSSWYMTVTQKTDPKMLSKKRTTSSQWQSPPVVVI
                     LKDMESFATKVLQDFIIISSQHLHEFPLILIFGIATSPIIIHRLLPHAVSSLLCIELF
                     QSLSCKEHLTTVLDKLLLTTQFPFKINEKVLQVLTNIFLYHDFSVQNFIKGLQLSLLE
                     HFYSQPLSVLCCNLPEAKRRINFLSNNQCENIRRLPSFRRYVEKQASEKQVALLTNER
                     YLKEETQLLLENLHVYHMNYFLVLRCLHKFTSSLPKYPLGRQIRELYCTCLEKNIWDS
                     EEYASVLQLLRMLAKDELMTILEKCFKVFKSYCENHLGSTAKRIEEFLAQFQSLDETK
                     EEEDASGSQPKGLQKTDLYHLQKSLLEMKELRRSKKQTKFEVLRENVVNFIDCLVREY
                     LLPPETQPLHEVVYFSAAHALREHLNAAPRIALHTALNNPYYYLKNEALKSEEGCIPN
                     IAPDICIAYKLHLECSRLINLVDWSEAFATVVTAAEKMDANSATSEEMNEIIHARFIR
                     AVSELELLGFIKPTKQKTDHVARLTWGGC"
     exon            59..113
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            114..211
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            212..356
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            357..461
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            462..613
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            614..747
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            748..907
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1021
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1155
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1219
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1336
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1416
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1550
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1627
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1725
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1867
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..1984
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2064
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2498
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2471..2476
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /note="hexamer: AATAAA"
     polyA_site      2498
                     /gene="ORC3"
                     /gene_synonym="LAT; LATHEO; ORC3L"
                     /note="major polyA site"
ORIGIN      
        1 gagtgcatct ggaatacgca gagtcagtaa gaccatggct acgtcctcga tgtctaaggg
       61 ttgctttgtt tttaagccaa actccaaaaa gagaaagatc tctctgccaa tagaggacta
      121 ttttaacaaa gggaaaaatg agcctgagga cagtaagctt cgattcgaaa cttatcagtt
      181 gatatggcag cagatgaaat ctgaaaatga gcgactacaa gaggaattaa ataaaaactt
      241 gtttgacaat ctgattgaat ttctgcaaaa atcacattct ggattccaga agaattcaag
      301 agacttgggc ggtcaaataa aactcagaga aattccaact gctgctcttg ttcttggtgt
      361 gaatgtcaca gatcatgatt tgacattcgg aagtctaaca gaggcccttc agaataatgt
      421 cacaccatat gtagtctcat tgcaagctaa agattgtcca gatatgaaac attttttgca
      481 aaagttgatc tcacagttga tggactgctg tgtagatata aaatccaaag aggaggaaag
      541 tgttcacgtc acccaaagaa agacacatta ttcaatggat tcactttcca gttggtatat
      601 gactgtcaca cagaagacgg acccaaaaat gctaagcaaa aaaaggacta cttctagcca
      661 atggcagtct cctcctgttg tcgttatctt gaaggatatg gaaagctttg ccacaaaagt
      721 actacaagac ttcataatta tcagcagtca acatctccat gaatttccac taatactcat
      781 ttttggaata gccacatctc ctattatcat ccaccgattg cttcctcatg cagtatcatc
      841 tctattgtgc atagaactgt tccaatcttt gtcttgtaag gagcacctga ctacggtact
      901 cgataagcta cttcttacaa ctcagtttcc ctttaaaata aatgaaaaag tattacaggt
      961 tctgaccaac atctttttgt atcatgattt ctcagttcaa aactttataa aaggacttca
     1021 gctttctcta ttagagcatt tctattccca gcccttaagt gtcctgtgct gtaatcttcc
     1081 agaagccaaa agaagaataa attttttatc aaataatcaa tgtgaaaaca tccgacgtct
     1141 accatctttt aggaggtacg tggaaaagca agcttcagaa aagcaagttg cgctcttgac
     1201 caatgagaga tatttgaagg aggaaacaca attattacta gaaaacctgc atgtttatca
     1261 tatgaattac ttcctggttt tgagatgtct tcataagttc acctcttctc ttcccaagta
     1321 tccactaggt cgacagatca gagagttgta ctgtacatgt ttagaaaaga acatatggga
     1381 ttcagaggag tatgcatcag tcttgcagct gctgaggatg ttggcaaagg atgaactgat
     1441 gaccatactt gagaaatgtt tcaaggtttt taagtcttat tgtgaaaacc accttggcag
     1501 cacagctaag agaatagagg agttcctggc ccagtttcag agcctcgatg aaaccaaaga
     1561 ggaagaagat gcttctgggt cacagccaaa ggggcttcag aagacagacc tctatcatct
     1621 tcagaagtcc ttattggaaa tgaaggagtt aagaagaagt aagaagcaaa ccaaatttga
     1681 agtactcaga gaaaatgttg tgaacttcat tgactgtcta gtgagagaat accttctgcc
     1741 tcctgagaca cagcctctcc atgaggtggt gtacttcagt gctgcccatg cccttcgtga
     1801 gcatttaaat gctgctccgc gaattgccct ccatactgca ctcaacaatc cttactatta
     1861 tctcaagaat gaagcactga aaagcgaaga aggctgcatt ccgaatatcg ccccagacat
     1921 ctgcatagca tacaaactgc acctagagtg tagcaggctc atcaacctcg tggactggtc
     1981 agaggctttt gcaacagttg tgacagctgc tgaaaaaatg gatgcaaatt ctgcaacctc
     2041 agaagaaatg aatgaaatta tccatgctcg gtttattaga gctgtttctg aactagaact
     2101 tttaggattt ataaaaccta ccaaacagaa gactgaccat gtggcaagac taacatgggg
     2161 aggctgctag aaagcaaata agcaaagcca gaactatcac atttagctta agagaaaaag
     2221 gtgaccagtc atatttacat atattagagg agcctgtttt gttgagaaga taaatgtgta
     2281 acccccattg atgtttaacc agaaaagtac attgctaacc ccaaacaggc atgtatcaaa
     2341 acacctgtgg agtactttag actccaacaa ataataatgt aactaaaact gctcacacat
     2401 tttactgtac tttccaaagt cattactaaa ttgtgagtaa atcattcttg aacttagagt
     2461 atgtaaatgt aataaattcc gttatccagg agtataaa
//
