LOCUS       NM_006190               4288 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens origin recognition complex subunit 2 (ORC2),
            transcript variant 1, mRNA.
ACCESSION   NM_006190
VERSION     NM_006190.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Shibata,E. and Dutta,A.
  TITLE     A human cancer cell line initiates DNA replication normally in the
            absence of ORC5 and ORC2 proteins
  JOURNAL   J Biol Chem 295 (50), 16949-16959 (2020)
   PUBMED   32989049
  REMARK    GeneRIF: A human cancer cell line initiates DNA replication
            normally in the absence of ORC5 and ORC2 proteins.
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Jaremko,M.J., On,K.F., Thomas,D.R., Stillman,B. and Joshua-Tor,L.
  TITLE     The dynamic nature of the human origin recognition complex revealed
            through five cryoEM structures
  JOURNAL   Elife 9, e58622 (2020)
   PUBMED   32808929
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4288)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4288)
  AUTHORS   Choi,S.G., Olivet,J., Cassonnet,P., Vidalain,P.O., Luck,K.,
            Lambourne,L., Spirohn,K., Lemmens,I., Dos Santos,M., Demeret,C.,
            Jones,L., Rangarajan,S., Bian,W., Coutant,E.P., Janin,Y.L., van der
            Werf,S., Trepte,P., Wanker,E.E., De Las Rivas,J., Tavernier,J.,
            Twizere,J.C., Hao,T., Hill,D.E., Vidal,M., Calderwood,M.A. and
            Jacob,Y.
  TITLE     Maximizing binary interactome mapping with a minimal number of
            assays
  JOURNAL   Nat Commun 10 (1), 3907 (2019)
   PUBMED   31467278
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4288)
  AUTHORS   Li,X., Sun,X.H., Xu,H.Y., Pan,H.S., Liu,Y. and He,L.
  TITLE     Circ_ORC2 enhances the regulatory effect of miR-19a on its target
            gene PTEN to affect osteosarcoma cell growth
  JOURNAL   Biochem Biophys Res Commun 514 (4), 1172-1178 (2019)
   PUBMED   31103262
  REMARK    GeneRIF: The circ_ORC2 binds with miR-19a and enhances its
            expression, thereby inhibiting downstream PTEN expression and
            activating Akt pathway to promote osteosarcoma cell growth and
            invasion.
REFERENCE   6  (bases 1 to 4288)
  AUTHORS   Ritzi,M., Baack,M., Musahl,C., Romanowski,P., Laskey,R.A. and
            Knippers,R.
  TITLE     Human minichromosome maintenance proteins and human origin
            recognition complex 2 protein on chromatin
  JOURNAL   J Biol Chem 273 (38), 24543-24549 (1998)
   PUBMED   9733749
REFERENCE   7  (bases 1 to 4288)
  AUTHORS   Saha,P., Thome,K.C., Yamaguchi,R., Hou,Z., Weremowicz,S. and
            Dutta,A.
  TITLE     The human homolog of Saccharomyces cerevisiae CDC45
  JOURNAL   J Biol Chem 273 (29), 18205-18209 (1998)
   PUBMED   9660782
REFERENCE   8  (bases 1 to 4288)
  AUTHORS   Quintana,D.G., Hou,Zh., Thome,K.C., Hendricks,M., Saha,P. and
            Dutta,A.
  TITLE     Identification of HsORC4, a member of the human origin of
            replication recognition complex
  JOURNAL   J Biol Chem 272 (45), 28247-28251 (1997)
   PUBMED   9353276
REFERENCE   9  (bases 1 to 4288)
  AUTHORS   Takahara,K., Bong,M., Brevard,R., Eddy,R.L., Haley,L.L., Sait,S.J.,
            Shows,T.B., Hoffman,G.G. and Greenspan,D.S.
  TITLE     Mouse and human homologues of the yeast origin of replication
            recognition complex subunit ORC2 and chromosomal localization of
            the cognate human gene ORC2L
  JOURNAL   Genomics 31 (1), 119-122 (1996)
   PUBMED   8808289
REFERENCE   10 (bases 1 to 4288)
  AUTHORS   Gavin,K.A., Hidaka,M. and Stillman,B.
  TITLE     Conserved initiator proteins in eukaryotes
  JOURNAL   Science 270 (5242), 1667-1671 (1995)
   PUBMED   7502077
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA111542.1, U40268.2,
            AI033876.1 and AC005037.2.
            
            On Nov 22, 2018 this sequence version replaced NM_006190.4.
            
            Summary: The origin recognition complex (ORC) is a highly conserved
            six subunits protein complex essential for the initiation of the
            DNA replication in eukaryotic cells. Studies in yeast demonstrated
            that ORC binds specifically to origins of replication and serves as
            a platform for the assembly of additional initiation factors such
            as Cdc6 and Mcm proteins. The protein encoded by this gene is a
            subunit of the ORC complex. This protein forms a core complex with
            ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are
            proteins known to be important for the initiation of DNA
            replication. This protein has been demonstrated to specifically
            associate with the origin of replication of Epstein-Barr virus in
            human cells, and is thought to be required for DNA replication from
            viral origin of replication. Alternatively spliced transcript
            variants have been found, one of which is a nonsense-mediated mRNA
            decay candidate. [provided by RefSeq, Oct 2010].
            
            Transcript Variant: This variant (1) encodes a functional protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.194191.1,
                                           SRR1803611.141728.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000234296.7/ ENSP00000234296.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                DA111542.1         42-85
            45-2773             U40268.2           1-2729
            2774-3094           AI033876.1         6-326               c
            3095-4288           AC005037.2         104316-105509
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q33.1"
     gene            1..4288
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /note="origin recognition complex subunit 2"
                     /db_xref="GeneID:4999"
                     /db_xref="HGNC:HGNC:8488"
                     /db_xref="MIM:601182"
     exon            1..171
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            172..220
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            221..324
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     CDS             231..1964
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /note="origin recognition complex, subunit 2 homolog;
                     origin recognition complex protein 2 homolog"
                     /codon_start=1
                     /product="origin recognition complex subunit 2"
                     /protein_id="NP_006181.1"
                     /db_xref="CCDS:CCDS2334.1"
                     /db_xref="GeneID:4999"
                     /db_xref="HGNC:HGNC:8488"
                     /db_xref="MIM:601182"
                     /translation="MSKPELKEDKMLEVHFVGDDDVLNHILDREGGAKLKKERAQLLV
                     NPKKIIKKPEYDLEEDDQEVLKDQNYVEIMGRDVQESLKNGSATGGGNKVYSFQNRKH
                     SEKMAKLASELAKTPQKSVSFSLKNDPEITINVPQSSKGHSASDKVQPKNNDKSEFLS
                     TAPRSLRKRLIVPRSHSDSESEYSASNSEDDEGVAQEHEEDTNAVIFSQKIQAQNRVV
                     SAPVGKETPSKRMKRDKTSDLVEEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLL
                     SKVSPSFSAELKQLNQQYEKLFHKWMLQLHLGFNIVLYGLGSKRDLLERFRTTMLQDS
                     IHVVINGFFPGISVKSVLNSITEEVLDHMGTFRSILDQLDWIVNKFKEDSSLELFLLI
                     HNLDSQMLRGEKSQQIIGQLSSLHNIYLIASIDHLNAPLMWDHAKQSLFNWLWYETTT
                     YSPYTEETSYENSLLVKQSGSLPLSSLTHVLRSLTPNARGIFRLLIKYQLDNQDNPSY
                     IGLSFQDFYQQCREAFLVNSDLTLRAQLTEFRDHKLIRTKKGTDGVEYLLIPVDNGTL
                     TDFLEKEEEEA"
     exon            325..468
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            469..558
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            559..651
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            652..683
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            684..744
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            745..938
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            939..1037
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1147
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1280
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1377
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1524
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1696
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1758
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1877
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..4288
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2761..2766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /note="hexamer: AATAAA"
     polyA_site      2785
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /note="major polyA site"
     regulatory      3074..3079
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /note="hexamer: AATAAA"
     polyA_site      3094
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /note="major polyA site"
     regulatory      4257..4262
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /note="hexamer: AATAAA"
     polyA_site      4288
                     /gene="ORC2"
                     /gene_synonym="ORC2L"
                     /note="major polyA site"
ORIGIN      
        1 atcccccagc tggcctccgg tgctccttac ctccccggtg gtcgggcgcg aattactgga
       61 aattggcttt tcccgttggg gccgaaggta ccttccctgc ggcggcgact cagcggggtg
      121 tcgttcggcc ggcgtgacgc agccggatcg gcgccagacg gaaacctagc ggtgactgta
      181 tctgaatttt gcagctgcag aatgtgtagt accttaaaag gttggcaaca atgagtaaac
      241 cagaattaaa ggaagacaag atgctggagg ttcactttgt gggagatgat gatgttctta
      301 atcacattct agatagagaa ggaggagcta aattgaagaa ggagcgagcg cagcttttgg
      361 tcaaccccaa aaaaataata aagaagccag aatatgattt ggaggaagat gaccaggagg
      421 tcttaaaaga tcagaactat gtggaaatta tgggaagaga tgttcaagaa tcattgaaaa
      481 atggctctgc tacaggtggt ggaaataaag tttattcttt tcagaataga aaacactctg
      541 aaaagatggc taaattagct tcagaactag caaaaacacc acaaaaaagt gtttcattca
      601 gtttgaagaa tgatcctgag attacgataa acgttcctca aagtagcaag ggccattctg
      661 cttcagacaa ggttcaaccg aagaacaatg acaaaagtga atttctgtca acagcacctc
      721 gtagtctaag aaaaagatta atagttccaa ggtctcattc tgacagtgaa agcgaatatt
      781 ctgcttccaa ctcagaggat gatgaagggg ttgcacagga acatgaagag gacactaatg
      841 cagtcatatt cagccaaaag attcaagctc agaatagagt agtttcagct cctgttggca
      901 aagaaacacc ttctaagaga atgaaaagag ataaaacaag tgacttagta gaagaatatt
      961 ttgaagctca cagcagttca aaagttttaa cctctgatag aacactgcag aagctaaaga
     1021 gagctaaact ggatcagcaa actttgcgta acttattgag caaggtttcc ccttcctttt
     1081 ctgccgaact taaacaacta aatcaacagt atgaaaaatt atttcataaa tggatgctgc
     1141 aattacacct tgggttcaac attgtgcttt atggtttggg ttctaagaga gatttactag
     1201 aaaggtttcg aaccactatg ctgcaagatt ccattcacgt tgtcatcaat ggcttctttc
     1261 ctggaatcag tgtgaaatca gtcctgaatt ctataacaga agaagtcctc gatcatatgg
     1321 gtactttccg cagtatactg gatcagctag actggatagt aaacaaattt aaagaagatt
     1381 cttctttaga actcttcctt ctcatccaca atttggatag ccagatgttg agaggagaga
     1441 agagccagca aatcattggt cagttgtcat ctttgcataa catttacctt atagcatcca
     1501 ttgaccacct caatgctcct ctcatgtggg atcatgcaaa gcagagtctt tttaactggc
     1561 tctggtatga aactactaca tacagtcctt atactgaaga aacctcctat gagaactctc
     1621 ttctggtaaa gcagtctgga tccctgccac ttagctccct tactcatgtc ttacgaagcc
     1681 ttacccctaa tgcaagggga attttcaggc tactaataaa ataccagctg gacaaccagg
     1741 ataacccttc ttacattggc ctttcttttc aagattttta ccagcagtgt cgggaggcat
     1801 tcctcgtcaa tagtgatctg acactccggg cccagttaac tgaatttagg gaccacaagc
     1861 ttataagaac aaagaaggga actgatggag tagagtattt attaattcct gttgataatg
     1921 gaacattgac tgatttcttg gaaaaggaag aagaggaggc ttgaagcttt cctttattct
     1981 tgaatctccc atggaagggt tgtaccccag ctgccactcc tctagttgaa agtgttgtgt
     2041 ttacatctga cattaaatta tttttccagc atacaagatt taaatttggg aaggggggga
     2101 tgtcctcaat tagaactttt tgatcagcct ggctggtacc gtctagtact atgcagcggt
     2161 cctcaagttg gagaaaatgt gcctttcatt cattacctct ctggagactt cttgctggaa
     2221 tgaacagtgt gctcagggac tatttggaac tggatgtttt tgaattattt tatacttaga
     2281 gatattctga attttttgag ggccttttaa cactccccga gctgattgtt tgcaagtgtg
     2341 tttgttccag agtgtggaag tataaagaca tgggcatcac gtaaattggt tttgtttgct
     2401 attctgtgtg tcagaaccaa cgagtgtaat ggagagggca ggtcatctct tattgtttct
     2461 aaaacaactt aaaaggtgta gattgggaag aggtgagtga tccagctttc tccttttgga
     2521 ttgaggctat gtacttggtg ggggcagggg agggaatata ttataatact attcagttgg
     2581 gataatggga aaaacagagt atatagggta tctacccagc ctagaaagca caggaacaat
     2641 acgtcatata tttggaacag ttattgtctg tgccatgacc ttcatgatac cagtgagaag
     2701 ccaggctaga gaaataaaat cctgaattac attttagtaa ttgttttcaa gacaacaaaa
     2761 aataaaacat ttcattattc ttgtatattg acatattttt cttttcttga acaacatgca
     2821 ggtgagtagc atattaatgg ctggctgtaa attaattttg attcctctgt caagtagacc
     2881 taaaactcac tgtaagatat ttcagttttt tccttaaagt gatgatttca aacctcctga
     2941 atatttgcgc gagacttttt tcttaggaaa gaaaaaaggc tcaccttggc tactgttgtc
     3001 agcttacagt catcacatac cccctgaaat atttgacact tggaaatgaa gttcttcctg
     3061 aaaatatcac tgcaataaaa tacgtactac gtcagcattt ctcactcatt tcttatcttg
     3121 taaacctctt tcatgaaaat atttccaaaa atccttggaa tttttaaata atttttcttg
     3181 tggcatataa ttaagtaatt ttggccaggc ataatggctt atgcctgtaa tcacaacact
     3241 ttgggaggcc aaggcaggag gatctctgga gcccaggagt ttaagaacag cctgggcaac
     3301 atagtgagac cttgtctcta caaaaaattt aaaaaatcat ccgggcatgg tggcccatgc
     3361 ctatagtcct agctgctgtg gaggctgagg caggaggatc gcttgagccc aggggttcaa
     3421 gattacagtg agctatgatt gtgccactgc acttcagtct gggtgcagag caagaccttg
     3481 tctttttttt tttttaaatc atttttttaa aaagtaattt tatgttaagt ccatgggctt
     3541 agtctttttt tttttttttt tttttttaga tggaattgca ctgtcatcca gtctagagtg
     3601 cagtggcgtg atctaagctt acagcagcct ccacctcctg ggttcaagtg attcttgtgc
     3661 ctcagcctcc tgagctggga ctacaggcac acgccaccac gcctggctag tttttgaatt
     3721 tttagcagag atggggtttc accatgttgg ccaggctggt ctcgaattcc tgacctcaag
     3781 tgatcctgcc tcggcctccc aaagtactgg gatcaggggt gagccactat gcctggccaa
     3841 gggcttagtc ttaaatataa cctctagtca aaatgtttaa acaaatttgt acataaatgt
     3901 gatttaaaaa aattttttta atagtaccaa aggatattca gtgaaagtga gtttcctgcc
     3961 cactccctga tccacccagt tctttaggca accactgata gcattttctt aagtattctt
     4021 ccagatatcg tctatgcata tgtaaaagta tctgtctttc tccttttaaa aacacaattg
     4081 gtaatatatc atacttgctg gtttgcacct tgcttttttt gcttaatata tctagtttat
     4141 aatgacctaa tgtgcaaatt tttgcatctg ccctaaatat ctactggttt atttttataa
     4201 tttttgtgtc tatactattt gcaagcagta tgtgtcagta gactttatta ttaataaata
     4261 aaaagccaat ttggatagaa tcacttta
//
