LOCUS       NM_021225                983 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens opiorphin prepropeptide (OPRPN), transcript variant 1,
            mRNA.
ACCESSION   NM_021225
VERSION     NM_021225.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Gavcar,E.G., Kabukcu Basay,B., Avci,E. and Basay,O.
  TITLE     Relationship between saliva opiorphin levels, pain threshold, and
            cutting number in adolescents with non suicidal self injury
  JOURNAL   J Psychiatr Res 151, 611-618 (2022)
   PUBMED   35636040
  REMARK    GeneRIF: Relationship between saliva opiorphin levels, pain
            threshold, and cutting number in adolescents with non suicidal self
            injury.
REFERENCE   2  (bases 1 to 983)
  AUTHORS   Mukherjee,A., Park,A., Wang,L. and Davies,K.P.
  TITLE     Role of opiorphin genes in prostate cancer growth and progression
  JOURNAL   Future Oncol 17 (17), 2209-2223 (2021)
   PUBMED   33593085
  REMARK    GeneRIF: Role of opiorphin genes in prostate cancer growth and
            progression.
REFERENCE   3  (bases 1 to 983)
  AUTHORS   Bosler,J.S., Davies,K.P. and Neal-Perry,G.S.
  TITLE     Peptides in seminal fluid and their role in infertility: a
            potential role for opiorphin inhibition of neutral endopeptidase
            activity as a clinically relevant modulator of sperm motility: a
            review
  JOURNAL   Reprod Sci 21 (11), 1334-1340 (2014)
   PUBMED   24855109
  REMARK    Review article
REFERENCE   4  (bases 1 to 983)
  AUTHORS   Benyhe,Z., Toth,G., Wollemann,M., Borsodi,A., Helyes,Z., Rougeot,C.
            and Benyhe,S.
  TITLE     Effects of synthetic analogues of human opiorphin on rat brain
            opioid receptors
  JOURNAL   J Physiol Pharmacol 65 (4), 525-530 (2014)
   PUBMED   25179084
REFERENCE   5  (bases 1 to 983)
  AUTHORS   Pinto,M., Rougeot,C., Gracia,L., Rosa,M., Garcia,A., Arsequell,G.,
            Valencia,G. and Centeno,N.B.
  TITLE     Proposed Bioactive Conformations of Opiorphin, an Endogenous Dual
            APN/NEP Inhibitor
  JOURNAL   ACS Med Chem Lett 3 (1), 20-24 (2011)
   PUBMED   24900367
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 983)
  AUTHORS   Thanawala,V., Kadam,V.J. and Ghosh,R.
  TITLE     Enkephalinase inhibitors: potential agents for the management of
            pain
  JOURNAL   Curr Drug Targets 9 (10), 887-894 (2008)
   PUBMED   18855623
  REMARK    Review article
REFERENCE   7  (bases 1 to 983)
  AUTHORS   Tong,Y., Tar,M., Melman,A. and Davies,K.
  TITLE     The opiorphin gene (ProL1) and its homologues function in erectile
            physiology
  JOURNAL   BJU Int 102 (6), 736-740 (2008)
   PUBMED   18410445
  REMARK    GeneRIF: All members of the human opiorphin family of genes can
            potentially modulate erectile physiology. Both hSMR3 and ProL1 are
            down-regulated in the corpora of men with ED, and therefore both
            genes can potentially act as markers of ED.
REFERENCE   8  (bases 1 to 983)
  AUTHORS   Wisner,A., Dufour,E., Messaoudi,M., Nejdi,A., Marcel,A.,
            Ungeheuer,M.N. and Rougeot,C.
  TITLE     Human Opiorphin, a natural antinociceptive modulator of
            opioid-dependent pathways
  JOURNAL   Proc Natl Acad Sci U S A 103 (47), 17979-17984 (2006)
   PUBMED   17101991
  REMARK    GeneRIF: Results report the discovery of opiorphin, a previously
            uncharacterized physiological inhibitor of enkephalin-inactivating
            zinc ectopeptidases in humans.
REFERENCE   9  (bases 1 to 983)
  AUTHORS   Ozyildirim,A.M., Wistow,G.J., Gao,J., Wang,J., Dickinson,D.P.,
            Frierson,H.F. Jr. and Laurie,G.W.
  TITLE     The lacrimal gland transcriptome is an unusually rich source of
            rare and poorly characterized gene transcripts
  JOURNAL   Invest Ophthalmol Vis Sci 46 (5), 1572-1580 (2005)
   PUBMED   15851553
REFERENCE   10 (bases 1 to 983)
  AUTHORS   Dickinson,D.P. and Thiesse,M.
  TITLE     cDNA cloning of an abundant human lacrimal gland mRNA encoding a
            novel tear protein
  JOURNAL   Curr Eye Res 15 (4), 377-386 (1996)
   PUBMED   8670737
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC092438.1 and CK430664.1.
            
            On Nov 23, 2018 this sequence version replaced NM_021225.4.
            
            Summary: This gene encodes a member of the proline-rich protein
            family. The encoded protein has multiple proposed functions,
            including roles in pain suppression, penile erection, and
            protection of the eye surface. The QRFSR pentapeptide, known as
            opiorphin, is derived from the N-terminal of this protein.
            Opiorphin inhibits the enkephalin-inactivating peptidases
            neprilysin and aminopeptidase N, and this activity is thought to
            reduce sensitivity to painful stimuli by effecting
            enkephalin-related activation of opioid-dependent pathways.
            Opiorphin may also act as an anti-depressant. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Nov
            2014].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC092438.1, S83198.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA2150585
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399575.7/ ENSP00000382485.2
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-981               BC092438.1         1-981
            982-983             CK430664.1         475-476
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q13.3"
     gene            1..983
                     /gene="OPRPN"
                     /gene_synonym="BPLP; opiorphin; PRL1; PROL1"
                     /note="opiorphin prepropeptide"
                     /db_xref="GeneID:58503"
                     /db_xref="HGNC:HGNC:17279"
                     /db_xref="MIM:608936"
     exon            1..101
                     /gene="OPRPN"
                     /gene_synonym="BPLP; opiorphin; PRL1; PROL1"
                     /inference="alignment:Splign:2.1.0"
     exon            102..167
                     /gene="OPRPN"
                     /gene_synonym="BPLP; opiorphin; PRL1; PROL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..863
                     /gene="OPRPN"
                     /gene_synonym="BPLP; opiorphin; PRL1; PROL1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; proline-rich 1; basic proline-rich protein;
                     proline-rich protein 1; basic proline-rich lacrimal
                     protein; proline rich, lacrimal 1"
                     /codon_start=1
                     /product="opiorphin prepropeptide isoform 1 precursor"
                     /protein_id="NP_067048.4"
                     /db_xref="CCDS:CCDS43235.1"
                     /db_xref="GeneID:58503"
                     /db_xref="HGNC:HGNC:17279"
                     /db_xref="MIM:608936"
                     /translation="MKLTFFLGLLALISCFTPSESQRFSRRPYLPGQLPPPPLYRPRW
                     VPPSPPPPYDSRLNSPLSLPFVPGRVPPSSFSRFSQAVILSQLFPLESIRQPRLFPGY
                     PNLHFPLRPYYVGPIRILKPPFPPIPFFLAIYLPISNPEPQINITTADTTITTNPPTT
                     ATATTSTSTKPTMTISSSTVPISSTPEPATSISAATPAASTENTTQILANRPHTVLLN
                     ATVQVTTSNQTILSSPAFKSFWQKLFAIFG"
     exon            168..983
                     /gene="OPRPN"
                     /gene_synonym="BPLP; opiorphin; PRL1; PROL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      870..875
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OPRPN"
                     /gene_synonym="BPLP; opiorphin; PRL1; PROL1"
                     /note="hexamer: AATAAA"
     polyA_site      889
                     /gene="OPRPN"
                     /gene_synonym="BPLP; opiorphin; PRL1; PROL1"
                     /note="major polyA site"
     regulatory      963..968
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OPRPN"
                     /gene_synonym="BPLP; opiorphin; PRL1; PROL1"
                     /note="hexamer: AATAAA"
     polyA_site      983
                     /gene="OPRPN"
                     /gene_synonym="BPLP; opiorphin; PRL1; PROL1"
                     /note="major polyA site"
ORIGIN      
        1 ctttctcttc ttggactttt aaattgtggc tacctaaatt gagtatctgg caagagtaag
       61 attaagcagt aatttgttcc aaagaagaat cttctaccaa ggagcaactt taaagaatga
      121 aattaacttt cttcttgggc ctgttggctc ttatttcatg tttcacaccc agtgagagtc
      181 aaagattctc cagaagacca tatctacctg gccagctgcc accacctcca ctctacaggc
      241 caagatgggt tccaccaagt cccccacctc cctatgactc aagacttaat tcaccacttt
      301 ctcttccctt tgtcccaggg cgagttccac catcttcttt ctctcgattt agccaagcag
      361 tcattctatc tcaactcttt ccattggaat ctattagaca acctcgactc tttccgggtt
      421 atccaaacct acatttccca ctaagacctt actatgtagg acctattagg atattaaaac
      481 ccccatttcc tcctattcct ttttttcttg ctatttacct tcctatctct aaccctgagc
      541 cccaaataaa catcaccacc gcagatacaa caatcaccac aaatcccccc accactgcaa
      601 cagcaaccac cagcacttcc acaaaaccca caatgacgat cagctcctca acagtaccta
      661 tctcttcaac accagagcct gccacctcca tatcagcagc aacccccgca gcatctactg
      721 aaaatactac tcaaattctc gccaaccgtc ctcacacagt attgctcaat gccactgtcc
      781 aagttacgac ttccaaccaa actatattaa gcagcccagc ctttaaaagt ttttggcaaa
      841 aactctttgc catttttggt tgaacatgca ataaatgata ttttccaaac tgctctgata
      901 tcttagaaga aataaactgc aatgattttg atggaaccaa ccctgatcta accagcacac
      961 taaataaagt atttgagcaa taa
//
