LOCUS       NM_000912               4954 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens opioid receptor kappa 1 (OPRK1), transcript variant 1,
            mRNA.
ACCESSION   NM_000912
VERSION     NM_000912.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Shi,Y., Tao,H., Li,X., Zhang,L., Li,C., Sun,W., Chu,M., Chen,K.,
            Zhu,P., Wang,Q., Gu,C., Wang,L., Yang,X. and Hao,Y.
  TITLE     kappa-Opioid receptor activation attenuates osteoarthritis
            synovitis by regulating macrophage polarization through the
            NF-kappaB pathway
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 56 (1), 82-95 (2024)
   PUBMED   38013468
  REMARK    GeneRIF: kappa-Opioid receptor activation attenuates osteoarthritis
            synovitis by regulating macrophage polarization through the
            NF-kappaB pathway.
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Ozkan-Kotiloglu,S., Kaya-Akyuzlu,D., Yurdakul,R., Yildirim,M.A. and
            Ozgur-Ilhan,I.
  TITLE     Effect of PDYN and OPRK1 polymorphisms on the risk of alcohol use
            disorder and the intensity of depressive symptoms
  JOURNAL   Alcohol Alcohol 58 (4), 404-414 (2023)
   PUBMED   37177778
  REMARK    GeneRIF: Effect of PDYN and OPRK1 polymorphisms on the risk of
            alcohol use disorder and the intensity of depressive symptoms.
REFERENCE   3  (bases 1 to 4954)
  AUTHORS   Hoye,J., Key,J., de Laat,B., Cosgrove,K.P., Krishnan-Sarin,S.,
            Papademetris,X. and Morris,E.D.
  TITLE     Clustering of KOR PET images separates people with AUD into
            distinct responses to naltrexone
  JOURNAL   Brain Imaging Behav 17 (3), 367-371 (2023)
   PUBMED   36695971
  REMARK    GeneRIF: Clustering of KOR PET images separates people with AUD
            into distinct responses to naltrexone.
REFERENCE   4  (bases 1 to 4954)
  AUTHORS   Loughran,G., Chou,M.Y., Ivanov,I.P., Jungreis,I., Kellis,M.,
            Kiran,A.M., Baranov,P.V. and Atkins,J.F.
  TITLE     Evidence of efficient stop codon readthrough in four mammalian
            genes
  JOURNAL   Nucleic Acids Res 42 (14), 8928-8938 (2014)
   PUBMED   25013167
REFERENCE   5  (bases 1 to 4954)
  AUTHORS   Yuferov,V., Fussell,D., LaForge,K.S., Nielsen,D.A., Gordon,D.,
            Ho,A., Leal,S.M., Ott,J. and Kreek,M.J.
  TITLE     Redefinition of the human kappa opioid receptor gene (OPRK1)
            structure and association of haplotypes with opiate addiction
  JOURNAL   Pharmacogenetics 14 (12), 793-804 (2004)
   PUBMED   15608558
  REMARK    GeneRIF: OPKR1 structure and association of haplotypes with opiate
            addiction was found to have empirical significance.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 4954)
  AUTHORS   Beier,H. and Grimm,M.
  TITLE     Misreading of termination codons in eukaryotes by natural nonsense
            suppressor tRNAs
  JOURNAL   Nucleic Acids Res 29 (23), 4767-4782 (2001)
   PUBMED   11726686
  REMARK    Review article
REFERENCE   7  (bases 1 to 4954)
  AUTHORS   Simonin,F., Gaveriaux-Ruff,C., Befort,K., Matthes,H., Lannes,B.,
            Micheletti,G., Mattei,M.G., Charron,G., Bloch,B. and Kieffer,B.
  TITLE     kappa-Opioid receptor in humans: cDNA and genomic cloning,
            chromosomal assignment, functional expression, pharmacology, and
            expression pattern in the central nervous system
  JOURNAL   Proc Natl Acad Sci U S A 92 (15), 7006-7010 (1995)
   PUBMED   7624359
REFERENCE   8  (bases 1 to 4954)
  AUTHORS   Zhu,J., Chen,C., Xue,J.C., Kunapuli,S., DeRiel,J.K. and
            Liu-Chen,L.Y.
  TITLE     Cloning of a human kappa opioid receptor from the brain
  JOURNAL   Life Sci 56 (9), PL201-PL207 (1995)
   PUBMED   7869844
REFERENCE   9  (bases 1 to 4954)
  AUTHORS   Wang,J.B., Johnson,P.S., Wu,J.M., Wang,W.F. and Uhl,G.R.
  TITLE     Human kappa opiate receptor second extracellular loop elevates
            dynorphin's affinity for human mu/kappa chimeras
  JOURNAL   J Biol Chem 269 (42), 25966-25969 (1994)
   PUBMED   7929306
REFERENCE   10 (bases 1 to 4954)
  AUTHORS   Mansson,E., Bare,L. and Yang,D.
  TITLE     Isolation of a human kappa opioid receptor cDNA from placenta
  JOURNAL   Biochem Biophys Res Commun 202 (3), 1431-1437 (1994)
   PUBMED   8060324
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY466378.1, U17298.1,
            BC099912.1, DA264471.1 and AK023198.1.
            
            On Nov 23, 2018 this sequence version replaced NM_000912.4.
            
            Summary: This gene encodes an opioid receptor, which is a member of
            the 7 transmembrane-spanning G protein-coupled receptor family. It
            functions as a receptor for endogenous ligands, as well as a
            receptor for various synthetic opioids. Ligand binding results in
            inhibition of adenylate cyclase activity and neurotransmitter
            release. This opioid receptor plays a role in the perception of
            pain and mediating the hypolocomotor, analgesic and aversive
            actions of synthetic opioids. Variations in this gene have also
            been associated with alcohol dependence and opiate addiction.
            Alternatively spliced transcript variants encoding different
            isoforms have been found for this gene. A recent study provided
            evidence for translational readthrough in this gene, and expression
            of an additional C-terminally extended isoform via the use of an
            alternative in-frame translation termination codon. [provided by
            RefSeq, Dec 2017].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes two isoforms, which result from the use of
            alternative in-frame translation termination codons. The shorter
            isoform (1) results from translation termination at the upstream
            UGA stop codon, while the longer isoform (1x) results from UGA stop
            codon readthrough to the downstream UGA termination codon (nt .
            This RefSeq represents the shorter isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC099912.1, SRR1803612.57217.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265572.8/ ENSP00000265572.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-259               AY466378.1         62-320
            260-1392            U17298.1           22-1154
            1393-3126           BC099912.1         1366-3099
            3127-3685           DA264471.1         2-560
            3686-4954           AK023198.1         1826-3094
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q11.23"
     gene            1..4954
                     /gene="OPRK1"
                     /gene_synonym="K-OR-1; KOP; KOR; KOR-1; KOR1; OPRK"
                     /note="opioid receptor kappa 1"
                     /db_xref="GeneID:4986"
                     /db_xref="HGNC:HGNC:8154"
                     /db_xref="MIM:165196"
     exon            1..190
                     /gene="OPRK1"
                     /gene_synonym="K-OR-1; KOP; KOR; KOR-1; KOR1; OPRK"
                     /inference="alignment:Splign:2.1.0"
     exon            191..495
                     /gene="OPRK1"
                     /gene_synonym="K-OR-1; KOP; KOR; KOR-1; KOR1; OPRK"
                     /inference="alignment:Splign:2.1.0"
     CDS             239..1381
                     /gene="OPRK1"
                     /gene_synonym="K-OR-1; KOP; KOR; KOR-1; KOR1; OPRK"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Opiate receptor, kappa-1; kappa-type opioid receptor;
                     kappa opioid receptor"
                     /codon_start=1
                     /product="kappa-type opioid receptor isoform 1"
                     /protein_id="NP_000903.2"
                     /db_xref="CCDS:CCDS6152.1"
                     /db_xref="GeneID:4986"
                     /db_xref="HGNC:HGNC:8154"
                     /db_xref="MIM:165196"
                     /translation="MDSPIQIFRGEPGPTCAPSACLPPNSSAWFPGWAEPDSNGSAGS
                     EDAQLEPAHISPAIPVIITAVYSVVFVVGLVGNSLVMFVIIRYTKMKTATNIYIFNLA
                     LADALVTTTMPFQSTVYLMNSWPFGDVLCKIVISIDYYNMFTSIFTLTMMSVDRYIAV
                     CHPVKALDFRTPLKAKIINICIWLLSSSVGISAIVLGGTKVREDVDVIECSLQFPDDD
                     YSWWDLFMKICVFIFAFVIPVLIIIVCYTLMILRLKSVRLLSGSREKDRNLRRITRLV
                     LVVVAVFVVCWTPIHIFILVEALGSTSHSTAALSSYYFCIALGYTNSSLNPILYAFLD
                     ENFKRCFRDFCFPLKMRMERQSTSRVRNTVQDPAYLRDIDGMNKPV"
     exon            496..848
                     /gene="OPRK1"
                     /gene_synonym="K-OR-1; KOP; KOR; KOR-1; KOR1; OPRK"
                     /inference="alignment:Splign:2.1.0"
     exon            849..4954
                     /gene="OPRK1"
                     /gene_synonym="K-OR-1; KOP; KOR; KOR-1; KOR1; OPRK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4923..4928
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OPRK1"
                     /gene_synonym="K-OR-1; KOP; KOR; KOR-1; KOR1; OPRK"
                     /note="hexamer: AATAAA"
     polyA_site      4954
                     /gene="OPRK1"
                     /gene_synonym="K-OR-1; KOP; KOR; KOR-1; KOR1; OPRK"
                     /note="major polyA site"
ORIGIN      
        1 agtagtggga gacgtgcgct gagaggcggg ggctgcgctc ggcggaacag cagccctcgg
       61 gcggagagcg gggccggggt ccgagagcag gtgatgccaa gagctgagcg ggactcgtga
      121 gcgcgcggtt cagcacctac cagggcgtcc cgtaaaaaac ctcgccttcg cctgtctctg
      181 ggaaccatag cgccgcaggt gccgcctgtc ctcgccttcc tgctgcaatc gccccaccat
      241 ggactccccg atccagatct tccgcgggga gccgggccct acctgcgccc cgagcgcctg
      301 cctgcccccc aacagcagcg cctggtttcc cggctgggcc gagcccgaca gcaacggcag
      361 cgccggctcg gaggacgcgc agctggagcc cgcgcacatc tccccggcca tcccggtcat
      421 catcacggcg gtctactccg tagtgttcgt cgtgggcttg gtgggcaact cgctggtcat
      481 gttcgtgatc atccgataca caaagatgaa gacagcaacc aacatttaca tatttaacct
      541 ggctttggca gatgctttag ttactacaac catgcccttt cagagtacgg tctacttgat
      601 gaattcctgg ccttttgggg atgtgctgtg caagatagta atttccattg attactacaa
      661 catgttcacc agcatcttca ccttgaccat gatgagcgtg gaccgctaca ttgccgtgtg
      721 ccaccccgtg aaggctttgg acttccgcac acccttgaag gcaaagatca tcaatatctg
      781 catctggctg ctgtcgtcat ctgttggcat ctctgcaata gtccttggag gcaccaaagt
      841 cagggaagac gtcgatgtca ttgagtgctc cttgcagttc ccagatgatg actactcctg
      901 gtgggacctc ttcatgaaga tctgcgtctt catctttgcc ttcgtgatcc ctgtcctcat
      961 catcatcgtc tgctacaccc tgatgatcct gcgtctcaag agcgtccggc tcctttctgg
     1021 ctcccgagag aaagatcgca acctgcgtag gatcaccaga ctggtcctgg tggtggtggc
     1081 agtcttcgtc gtctgctgga ctcccattca catattcatc ctggtggagg ctctggggag
     1141 cacctcccac agcacagctg ctctctccag ctattacttc tgcatcgcct taggctatac
     1201 caacagtagc ctgaatccca ttctctacgc ctttcttgat gaaaacttca agcggtgttt
     1261 ccgggacttc tgctttccac tgaagatgag gatggagcgg cagagcacta gcagagtccg
     1321 aaatacagtt caggatcctg cttacctgag ggacatcgat gggatgaata aaccagtatg
     1381 actagtcgtg gagatgtctt cgtacagttc ttcgggaaga gaggagttca atgatctagg
     1441 tttaactcag atcactactg cagtctgaca tgaaaagata gaatttaaaa taaactttta
     1501 gagatctcat ggtctgaagt catcagatgc agaccacgtt cgtgatcagt ggaaacatca
     1561 aggacaaaag gtccacgcaa cacatgcacc tcttctctag ggcacagaag cgaggcagag
     1621 cctcagttcc tctgatgaac aagggaacag gtcttttcct tctggtttgc taggtaaggt
     1681 tcagcaccca tctgctgtgg ccttcctatg aaacgtagtt tcaaaagctc ggtttaagaa
     1741 aaaaggaaaa gaaaaaacat tgctttcaga gctcaatggg catccaatcc agatgtttca
     1801 caatgacagc aacaacctcc aacggaatgg gaactatttc cagggttcat gcccagctca
     1861 ctgtatgctg tgtggacatg catttcatgt ggctgtgtgg taagaattac agcttacata
     1921 tggcttggac ccacatctga ggaatctgat gttcacttat accagaacat tatcttgcta
     1981 tttatgaaat tatttaaacg ttcaaaagat tgtttttaac atggtttaat ttcccaaaaa
     2041 ctacagtttt ttttcttagc atgctattca ggtaaacagt cttataataa agcatgtccc
     2101 attgtcaaga aacataaagt ggtgtgaata ccactgaaaa tatatatata gtatcttctg
     2161 taaataatag tacctgtgtg aataaggaat aggcttgcct cccagccagg caatttcctg
     2221 agggcacact aatgatatcc cctgagttgc taagttgatg ctgagacatt ttgctgggaa
     2281 ttagtcatgg catgatctct ttcagactcc ctgaatacca tttagtcccg taacagtgct
     2341 cacaactcat gtgctaatga atcacaaagg ctttaactag ctcccaggtt gtagccttcg
     2401 caggatctag tttatttgcc acatctcttt atgaacatat agcgattcgc agatctctct
     2461 attcacggag aggaaggtgt tttgcttctg tagatctcaa ggtactattt tgtggctctc
     2521 agcaggaagt agaattgttc ctaaatgtgt gctgaatgag gacatgatgt ccctcctggt
     2581 gccaggacac atcctgcatg gcattctgtg aagggcatac ctgctgagga taatgccagg
     2641 agcagcacat ttagggtaat tttgctaaaa cttccagatg acatataaat tccctctttt
     2701 tccccctgaa acttaccatt tcagaggtaa tggctttttc tgtaatttgc ctgagaagaa
     2761 aaaagggata atttagaaat aacactccaa gcctatatat tactagggct gcatcctctg
     2821 gaatttaata gaaaaattga atatatagcc ctatgtaact acagacatca ttacaactgt
     2881 taagttctgt tcaccatgca agttctcggt ggtgtatgta ctctcacctt ttggaaacaa
     2941 aaccttgcat tgaaggtttt acacatggct tcagaatgtt actctgacca tgtttgcctc
     3001 tactccagat tatcatggca cctaacacag atgacatatg gcagaatgga agtgatttct
     3061 cctaatgaaa atgaagtgct cttttgagta tttcttgttt tattgagatt cctatctgcc
     3121 cttgtatttt tataactgaa tgtaaatagg aattgcttaa atactggttt acactaatcc
     3181 catagttaaa aatcttggct atggtacagg aaggatatct atatagagga tagttccaga
     3241 aacaagccct ggtgaaatag gaaatataaa aacttcttta tggaacagga aaacccaaga
     3301 gactttctcg tcagattcct ttccagtgca aagtataaca gagataggat tatgagatga
     3361 aatcacagta gtgtgtttcc tttaattttt tctgtcttct tttttttttt tttttttttt
     3421 gagacggagt ctcgctctgt tgcccaggct ggagtgcggt agcacagtct tggctcactg
     3481 caacctccac ctcctgggtt caagcgattc tcctgcctct gcctcctaag tagctgggat
     3541 tacaggcgtg caccgccaag cccggctaat ttttgtattt ttagtagaga cggggtttca
     3601 ccatgttatg caggctggtc tcaaactcct gacctcagaa tattcacctg cctcggcctc
     3661 ccaaagtgct gggattacag atgtgaatgg ccacgcctgg cctgtttcct ttaagttagc
     3721 acttcacgtg ctcttacagc gttaattgcc attattggtt tgcctgattt tcctgcatct
     3781 cctcatatgt aagtctgcag cccaaggaga gaatagtagc tgtatgtgcc cacaaggggt
     3841 gtccaagctt cttgattctt gttaatatcg actccccaac attgaaaaag gagtgaagaa
     3901 gggaatatca ttttagggta ctgctttaaa ggaatgataa agtaaacaac gtagcaggga
     3961 aaataccact tgcacaaatg tatttctttg tcaaagtgtg tacacgcttt ctgtttgtcc
     4021 tgatgtcctg ctgtgaagca gggaccacat tgccatttat tcattgttcc aggcaatgca
     4081 tttgtccaaa cccctctggc tctctgatat ctctcatttc ttgatgttcc tattctctga
     4141 cctcaacaag gaggacactg tctgttgtgt ctctttgaga ctagaactcc atgtgtttaa
     4201 ccttcagatc cattctctac atgaacccta ttctttccac cctggtaaga aggtctggaa
     4261 gacaggtcct aacgaggggt cagagaaggc acagagaaat gaaccagcca tgcaggggca
     4321 gcactgagga ggaaaccccc cactctaact gggaggagag tggaaacact cttagtatct
     4381 cagagtagtt accaatctaa gagcatcctt cgagtttaag tcaaaactct aggcttaata
     4441 ccaaaaataa tacatcattt ggaaaaatag cttggagaaa aaaaatcaat aaagagtcac
     4501 ataaaatctt aagaaaatgg ttctattctg tgatatatta ctaagaaata cagttcttaa
     4561 acgtgtataa ctattgtcag acaatttata ggtgtttcat ctagtcctgg gatgaaatac
     4621 gatgactcat gcaaattcag ggagcacact ggctgacttt aaattggaac attgtaaagt
     4681 gaaagctgtg aaaggttgtg tgtcctatca atgagattta ttttctaacc aagagctgtt
     4741 cggatgattc agtataaata cgaaaatcac atccaaaaac ggtataaccc agcttcctta
     4801 aggcaatttt cttctctgaa acaagaatat actcatatgt tctttactat aatgtatatg
     4861 atttttatct tgtactttaa aagatgataa ttatgcattg tatatacgat tgtgtgcctt
     4921 gcaataaaat caaaactgta cctgctgaaa atca
//
