LOCUS       NP_000903                380 aa            linear   PRI 27-APR-2025
DEFINITION  kappa-type opioid receptor isoform 1 [Homo sapiens].
ACCESSION   NP_000903
VERSION     NP_000903.2
DBSOURCE    REFSEQ: accession NM_000912.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 380)
  AUTHORS   Shi,Y., Tao,H., Li,X., Zhang,L., Li,C., Sun,W., Chu,M., Chen,K.,
            Zhu,P., Wang,Q., Gu,C., Wang,L., Yang,X. and Hao,Y.
  TITLE     kappa-Opioid receptor activation attenuates osteoarthritis
            synovitis by regulating macrophage polarization through the
            NF-kappaB pathway
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 56 (1), 82-95 (2024)
   PUBMED   38013468
  REMARK    GeneRIF: kappa-Opioid receptor activation attenuates osteoarthritis
            synovitis by regulating macrophage polarization through the
            NF-kappaB pathway.
REFERENCE   2  (residues 1 to 380)
  AUTHORS   Ozkan-Kotiloglu,S., Kaya-Akyuzlu,D., Yurdakul,R., Yildirim,M.A. and
            Ozgur-Ilhan,I.
  TITLE     Effect of PDYN and OPRK1 polymorphisms on the risk of alcohol use
            disorder and the intensity of depressive symptoms
  JOURNAL   Alcohol Alcohol 58 (4), 404-414 (2023)
   PUBMED   37177778
  REMARK    GeneRIF: Effect of PDYN and OPRK1 polymorphisms on the risk of
            alcohol use disorder and the intensity of depressive symptoms.
REFERENCE   3  (residues 1 to 380)
  AUTHORS   Hoye,J., Key,J., de Laat,B., Cosgrove,K.P., Krishnan-Sarin,S.,
            Papademetris,X. and Morris,E.D.
  TITLE     Clustering of KOR PET images separates people with AUD into
            distinct responses to naltrexone
  JOURNAL   Brain Imaging Behav 17 (3), 367-371 (2023)
   PUBMED   36695971
  REMARK    GeneRIF: Clustering of KOR PET images separates people with AUD
            into distinct responses to naltrexone.
REFERENCE   4  (residues 1 to 380)
  AUTHORS   Loughran,G., Chou,M.Y., Ivanov,I.P., Jungreis,I., Kellis,M.,
            Kiran,A.M., Baranov,P.V. and Atkins,J.F.
  TITLE     Evidence of efficient stop codon readthrough in four mammalian
            genes
  JOURNAL   Nucleic Acids Res 42 (14), 8928-8938 (2014)
   PUBMED   25013167
REFERENCE   5  (residues 1 to 380)
  AUTHORS   Yuferov,V., Fussell,D., LaForge,K.S., Nielsen,D.A., Gordon,D.,
            Ho,A., Leal,S.M., Ott,J. and Kreek,M.J.
  TITLE     Redefinition of the human kappa opioid receptor gene (OPRK1)
            structure and association of haplotypes with opiate addiction
  JOURNAL   Pharmacogenetics 14 (12), 793-804 (2004)
   PUBMED   15608558
  REMARK    GeneRIF: OPKR1 structure and association of haplotypes with opiate
            addiction was found to have empirical significance.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (residues 1 to 380)
  AUTHORS   Beier,H. and Grimm,M.
  TITLE     Misreading of termination codons in eukaryotes by natural nonsense
            suppressor tRNAs
  JOURNAL   Nucleic Acids Res 29 (23), 4767-4782 (2001)
   PUBMED   11726686
  REMARK    Review article
REFERENCE   7  (residues 1 to 380)
  AUTHORS   Simonin,F., Gaveriaux-Ruff,C., Befort,K., Matthes,H., Lannes,B.,
            Micheletti,G., Mattei,M.G., Charron,G., Bloch,B. and Kieffer,B.
  TITLE     kappa-Opioid receptor in humans: cDNA and genomic cloning,
            chromosomal assignment, functional expression, pharmacology, and
            expression pattern in the central nervous system
  JOURNAL   Proc Natl Acad Sci U S A 92 (15), 7006-7010 (1995)
   PUBMED   7624359
REFERENCE   8  (residues 1 to 380)
  AUTHORS   Zhu,J., Chen,C., Xue,J.C., Kunapuli,S., DeRiel,J.K. and
            Liu-Chen,L.Y.
  TITLE     Cloning of a human kappa opioid receptor from the brain
  JOURNAL   Life Sci 56 (9), PL201-PL207 (1995)
   PUBMED   7869844
REFERENCE   9  (residues 1 to 380)
  AUTHORS   Wang,J.B., Johnson,P.S., Wu,J.M., Wang,W.F. and Uhl,G.R.
  TITLE     Human kappa opiate receptor second extracellular loop elevates
            dynorphin's affinity for human mu/kappa chimeras
  JOURNAL   J Biol Chem 269 (42), 25966-25969 (1994)
   PUBMED   7929306
REFERENCE   10 (residues 1 to 380)
  AUTHORS   Mansson,E., Bare,L. and Yang,D.
  TITLE     Isolation of a human kappa opioid receptor cDNA from placenta
  JOURNAL   Biochem Biophys Res Commun 202 (3), 1431-1437 (1994)
   PUBMED   8060324
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY466378.1, U17298.1,
            BC099912.1, DA264471.1 and AK023198.1.
            
            On Dec 11, 2003 this sequence version replaced NP_000903.1.
            
            Summary: This gene encodes an opioid receptor, which is a member of
            the 7 transmembrane-spanning G protein-coupled receptor family. It
            functions as a receptor for endogenous ligands, as well as a
            receptor for various synthetic opioids. Ligand binding results in
            inhibition of adenylate cyclase activity and neurotransmitter
            release. This opioid receptor plays a role in the perception of
            pain and mediating the hypolocomotor, analgesic and aversive
            actions of synthetic opioids. Variations in this gene have also
            been associated with alcohol dependence and opiate addiction.
            Alternatively spliced transcript variants encoding different
            isoforms have been found for this gene. A recent study provided
            evidence for translational readthrough in this gene, and expression
            of an additional C-terminally extended isoform via the use of an
            alternative in-frame translation termination codon. [provided by
            RefSeq, Dec 2017].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes two isoforms, which result from the use of
            alternative in-frame translation termination codons. The shorter
            isoform (1) results from translation termination at the upstream
            UGA stop codon, while the longer isoform (1x) results from UGA stop
            codon readthrough to the downstream UGA termination codon (nt .
            This RefSeq represents the shorter isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC099912.1, SRR1803612.57217.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265572.8/ ENSP00000265572.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q11.23"
     Protein         1..380
                     /product="kappa-type opioid receptor isoform 1"
                     /note="Opiate receptor, kappa-1; kappa-type opioid
                     receptor; kappa opioid receptor"
                     /calculated_mol_wt=42514
     Site            25
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:17711303; propagated from
                     UniProtKB/Swiss-Prot (P41145.2)"
     Site            39
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:17711303; propagated from
                     UniProtKB/Swiss-Prot (P41145.2)"
     Site            58..85
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P41145.2)"
     Region          60..341
                     /region_name="7tmA_Kappa_opioid_R"
                     /note="opioid receptor subtype kappa, member of the class
                     A family of seven-transmembrane G protein-coupled
                     receptors; cd15091"
                     /db_xref="CDD:320219"
     Region          61..87
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320219"
     Region          94..120
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320219"
     Site            96..119
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P41145.2)"
     Site            order(115,118..119,131..136,138..139,142,187,189..193,223,
                     226..228,230..232,234..235,287,290..291,293..294,297,
                     308..309,311..313,316,319..320)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:320219"
     Region          131..161
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320219"
     Site            133..154
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P41145.2)"
     Region          173..195
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320219"
     Site            174..196
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P41145.2)"
     Region          223..252
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320219"
     Site            223..247
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P41145.2)"
     Region          267..297
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320219"
     Site            275..296
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P41145.2)"
     Region          309..334
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320219"
     Site            312..333
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P41145.2)"
     CDS             1..380
                     /gene="OPRK1"
                     /gene_synonym="K-OR-1; KOP; KOR; KOR-1; KOR1; OPRK"
                     /coded_by="NM_000912.5:239..1381"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS6152.1"
                     /db_xref="GeneID:4986"
                     /db_xref="HGNC:HGNC:8154"
                     /db_xref="MIM:165196"
ORIGIN      
        1 mdspiqifrg epgptcapsa clppnssawf pgwaepdsng sagsedaqle pahispaipv
       61 iitavysvvf vvglvgnslv mfviirytkm ktatniyifn laladalvtt tmpfqstvyl
      121 mnswpfgdvl ckivisidyy nmftsiftlt mmsvdryiav chpvkaldfr tplkakiini
      181 ciwllsssvg isaivlggtk vredvdviec slqfpdddys wwdlfmkicv fifafvipvl
      241 iiivcytlmi lrlksvrlls gsrekdrnlr ritrlvlvvv avfvvcwtpi hifilvealg
      301 stshstaals syyfcialgy tnsslnpily afldenfkrc frdfcfplkm rmerqstsrv
      361 rntvqdpayl rdidgmnkpv
//
