LOCUS       NM_002547               7569 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens oligophrenin 1 (OPHN1), mRNA.
ACCESSION   NM_002547
VERSION     NM_002547.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Yang,F. and Wang,M.
  TITLE     Effect of the OPHN1 novel variant c.1025+1 G>A on RNA splicing:
            insights from a minigene assay
  JOURNAL   BMC Med Genomics 17 (1), 175 (2024)
   PUBMED   38956616
  REMARK    GeneRIF: Effect of the OPHN1 novel variant c.1025+1 G>A on RNA
            splicing: insights from a minigene assay.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   Liu,J., Zhang,Y., Li,S., Sun,F., Wang,G., Wei,D., Yang,T. and Gu,S.
  TITLE     Androgen deprivation-induced OPHN1 amplification promotes
            castration-resistant prostate cancer
  JOURNAL   Oncol Rep 47 (1) (2022)
   PUBMED   34738630
  REMARK    GeneRIF: Androgen deprivationinduced OPHN1 amplification promotes
            castrationresistant prostate cancer.
REFERENCE   3  (bases 1 to 7569)
  AUTHORS   Nuovo,S., Brankovic,V., Caputi,C., Casella,A., Nigro,V., Leuzzi,V.
            and Valente,E.M.
  TITLE     Novel unconventional variants expand the allelic spectrum of OPHN1
            gene
  JOURNAL   Am J Med Genet A 185 (5), 1575-1581 (2021)
   PUBMED   33638601
  REMARK    GeneRIF: Novel unconventional variants expand the allelic spectrum
            of OPHN1 gene.
REFERENCE   4  (bases 1 to 7569)
  AUTHORS   Busa,T., Caietta,E., Chabrol,B., Girard,N., Philip,N. and
            Missirian,C.
  TITLE     Large in-frame intragenic deletion of OPHN1 in a male patient with
            a normal intelligence quotient score
  JOURNAL   Clin Dysmorphol 26 (1), 47-49 (2017)
   PUBMED   27390894
  REMARK    GeneRIF: we reported on the first male patient carrying an OPHN1
            mutation with IQ score within the normal limits. This observation
            expands the phenotypic spectrum of OPHN1 mutations.
REFERENCE   5  (bases 1 to 7569)
  AUTHORS   Redolfi,N., Galla,L., Maset,A., Murru,L., Savoia,E., Zamparo,I.,
            Gritti,A., Billuart,P., Passafaro,M. and Lodovichi,C.
  TITLE     Oligophrenin-1 regulates number, morphology and synaptic properties
            of adult-born inhibitory interneurons in the olfactory bulb
  JOURNAL   Hum Mol Genet 25 (23), 5198-5211 (2016)
   PUBMED   27742778
  REMARK    GeneRIF: Furthermore, we found that olfactory behaviour was
            perturbed in OPHN1 ko mice. Chronic treatment with a Rho kinase
            inhibitor rescued most of the defects of the newly generated
            neurons. Altogether, our data indicated that OPHN1 plays a key role
            in regulating the number, morphology and function of adult-born
            inhibitory interneurons and contributed to identify potential
            therapeutic targets.
REFERENCE   6  (bases 1 to 7569)
  AUTHORS   Billuart,P., Chelly,J., Carrie,A., Vinet,M., Couvert,P.,
            McDonell,N., Zemni,R., Kahn,A., Moraine,C., Beldjord,C. and
            Bienvenu,T.
  TITLE     Determination of the gene structure of human oligophrenin-1 and
            identification of three novel polymorphisms by screening of DNA
            from 164 patients with non-specific X-linked mental retardation
  JOURNAL   Ann Genet 43 (1), 5-9 (2000)
   PUBMED   10818214
REFERENCE   7  (bases 1 to 7569)
  AUTHORS   Tentler,D., Gustavsson,P., Leisti,J., Schueler,M., Chelly,J.,
            Timonen,E., Anneren,G., Willard,H.F. and Dahl,N.
  TITLE     Deletion including the oligophrenin-1 gene associated with enlarged
            cerebral ventricles, cerebellar hypoplasia, seizures and ataxia
  JOURNAL   Eur J Hum Genet 7 (5), 541-548 (1999)
   PUBMED   10439959
REFERENCE   8  (bases 1 to 7569)
  AUTHORS   Billuart,P., Bienvenu,T., Ronce,N., des Portes,V., Vinet,M.C.,
            Zemni,R., Roest Crollius,H., Carrie,A., Fauchereau,F., Cherry,M.,
            Briault,S., Hamel,B., Fryns,J.P., Beldjord,C., Kahn,A., Moraine,C.
            and Chelly,J.
  TITLE     Oligophrenin-1 encodes a rhoGAP protein involved in X-linked mental
            retardation
  JOURNAL   Nature 392 (6679), 923-926 (1998)
   PUBMED   9582072
REFERENCE   9  (bases 1 to 7569)
  AUTHORS   Bienvenu,T., Der-Sarkissian,H., Billuart,P., Tissot,M., Des
            Portes,V., Bruls,T., Chabrolle,J.P., Chauveau,P., Cherry,M.,
            Kahn,A., Cohen,D., Beldjord,C., Chelly,J. and Cherif,D.
  TITLE     Mapping of the X-breakpoint involved in a balanced X;12
            translocation in a female with mild mental retardation
  JOURNAL   Eur J Hum Genet 5 (2), 105-109 (1997)
   PUBMED   9195162
REFERENCE   10 (bases 1 to 7569)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL672138.10, BP357826.1,
            AJ001189.1 and AL157700.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 24, 2018 this sequence version replaced NM_002547.2.
            
            Summary: This gene encodes a Rho-GTPase-activating protein that
            promotes GTP hydrolysis of Rho subfamily members. Rho proteins are
            important mediators of intracellular signal transduction, which
            affects cell migration and cell morphogenesis. Mutations in this
            gene are responsible for OPHN1-related X-linked cognitive
            disability with cerebellar hypoplasia and distinctive facial
            dysmorhphism. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ001189.1, SRR14038194.1718848.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355520.6/ ENSP00000347710.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AL672138.10        97865-97902         c
            39-620              BP357826.1         1-582
            621-3542            AJ001189.1         927-3848
            3543-7569           AL157700.13        168587-172613       c
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq12"
     gene            1..7569
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /note="oligophrenin 1"
                     /db_xref="GeneID:4983"
                     /db_xref="HGNC:HGNC:8148"
                     /db_xref="MIM:300127"
     exon            1..328
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            329..486
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     CDS             333..2741
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /note="oligophrenin-1, Rho-GTPase activating protein"
                     /codon_start=1
                     /product="oligophrenin-1"
                     /protein_id="NP_002538.1"
                     /db_xref="CCDS:CCDS14388.1"
                     /db_xref="GeneID:4983"
                     /db_xref="HGNC:HGNC:8148"
                     /db_xref="MIM:300127"
                     /translation="MGHPPLEFSDCYLDSPDFRERLKCYEQELERTNKFIKDVIKDGN
                     ALISAMRNYSSAVQKFSQTLQSFQFDFIGDTLTDDEINIAESFKEFAELLNEVENERM
                     MMVHNASDLLIKPLENFRKEQIGFTKERKKKFEKDGERFYSLLDRHLHLSSKKKESQL
                     QEADLQVDKERHNFFESSLDYVYQIQEVQESKKFNIVEPVLAFLHSLFISNSLTVELT
                     QDFLPYKQQLQLSLQNTRNHFSSTREEMEELKKRMKEAPQTCKLPGQPTIEGYLYTQE
                     KWALGISWVKYYCQYEKETKTLTMTPMEQKPGAKQGPLDLTLKYCVRRKTESIDKRFC
                     FDIETNERPGTITLQALSEANRRLWMEAMDGKEPIYHSPITKQQEMELNEVGFKFVRK
                     CINIIETKGIKTEGLYRTVGSNIQVQKLLNAFFDPKCPGDVDFHNSDWDIKTITSSLK
                     FYLRNLSEPVMTYRLHKELVSAAKSDNLDYRLGAIHSLVYKLPEKNREMLELLIRHLV
                     NVCEHSKENLMTPSNMGVIFGPTLMRAQEDTVAAMMNIKFQNIVVEILIEHFGKIYLG
                     PPEESAAPPVPPPRVTARRHKPITISKRLLRERTVFYTSSLDESEDEIQHQTPNGTIT
                     SSIEPPKPPQHPKLPIQRSGETDPGRKSPSRPILDGKLEPCPEVDVGKLVSRLQDGGT
                     KITPKATNGPMPGSGPTKTPSFHIKRPAPRPLAHHKEGDADSFSKVRPPGEKPTIIRP
                     PVRPPDPPCRAATPQKPEPKPDIVAGNAGEITSSVVASRTRFFETASRKTGSSQGRLP
                     GDES"
     exon            487..582
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            583..644
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            645..716
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            717..818
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            819..929
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1034
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1164
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1265
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1266..1357
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1436
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1470
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1533
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1608
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1693
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1752
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1858
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..2018
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2166
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2490
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2491..2656
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2657..2707
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2708..2749
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2750..7569
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7542..7547
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /note="hexamer: AATAAA"
     polyA_site      7569
                     /gene="OPHN1"
                     /gene_synonym="ARHGAP41; MRX60; MRXSBL; OPN1"
                     /note="major polyA site"
ORIGIN      
        1 aaggaagtct tccgctcgct ccggagcgag gagcctgtag agaggctgtt cccagctcca
       61 gctctaacct cgcctacacc ttgggcgggc ccaagtgtca cgtttgcaat tgctcaggaa
      121 ggatccggcc cgtctccgga ggcaagtcgg gctgcggttt ttgctgctta tctgggaagg
      181 cgatgcctaa gggacatgct gcttgctagg cagcaccctg ccgggatccg actgcgatag
      241 ttagctctcc ctggccctga agccatcgcc ggggcgcctg ttctctgtcc ggaccagcca
      301 gcgctcctca ggagtctcac tgaaacagaa ccatgggtca tcccccgctg gagttcagcg
      361 actgctacct ggacagcccc gatttccgcg agaggctcaa gtgttatgag caggaactgg
      421 agaggaccaa caaattcatc aaagacgtaa tcaaagacgg caacgcgctt atcagcgcta
      481 tgagaaatta ttcttctgct gttcagaaat tttcccagac gctgcagtca tttcagtttg
      541 atttcattgg agacactctg actgatgatg aaattaacat cgctgaatcc ttcaaggaat
      601 ttgctgaatt gctcaacgag gtagaaaatg agaggatgat gatggtacac aatgctagtg
      661 atttgctgat taaacccttg gaaaatttcc ggaaggaaca aataggcttc accaaggagc
      721 ggaaaaagaa atttgaaaag gatggtgaga ggttttattc tttactggat cggcacttac
      781 acctgtcttc aaaaaagaaa gaatctcagt tacaagaggc agacctacag gtggacaagg
      841 agaggcacaa ttttttcgag tcctctcttg attatgttta tcaaatccag gaagttcagg
      901 agtccaagaa gttcaatatt gtggagcctg tcttggcctt tcttcatagt ctgttcattt
      961 ctaacagcct gactgtggag ctcacacagg atttcctccc atacaaacaa cagctccaac
     1021 tcagtttaca gaatacaaga aatcatttct ccagtacccg ggaagagatg gaagaactta
     1081 agaaaaggat gaaagaagct ccccagacat gcaaacttcc aggacagcca actattgaag
     1141 gctatctcta tacacaagag aaatgggctt taggaatatc ctgggtgaaa tactattgcc
     1201 agtatgagaa agagaccaaa acactgacca tgacgcctat ggagcagaag ccaggtgcta
     1261 agcaggggcc cttggactta acactgaagt actgtgtgag aaggaagacg gagtctatcg
     1321 acaagaggtt ctgttttgac atagaaacta atgaaaggcc aggaaccatc actctgcagg
     1381 ccctttcaga agctaacaga aggctatgga tggaagccat ggatgggaaa gaacctatct
     1441 accacagccc tataacaaaa cagcaagaaa tggagctaaa tgaagtgggc ttcaagtttg
     1501 tcaggaagtg catcaatatt attgagacca aagggatcaa gacagaaggg ttgtaccgca
     1561 ctgtgggcag caatattcag gttcagaagc tgctgaatgc cttttttgat cctaaatgcc
     1621 caggagatgt tgattttcat aatagtgact gggacattaa gacaatcacc agctccttga
     1681 aattctacct caggaatctt tctgaacctg tcatgaccta tagacttcac aaagagctgg
     1741 tctctgctgc caagtctgac aacctggatt accgcctagg agctattcac tccctggtat
     1801 ataagctacc agaaaagaac cgagagatgc tggaacttct gataagacac ttggtcaatg
     1861 tgtgtgagca cagcaaagag aatcttatga ccccctccaa catgggagta atctttgggc
     1921 ccaccctgat gagagctcag gaggacactg tggccgccat gatgaacatc aaattccaga
     1981 acatagtggt ggaaatacta atcgagcact ttggcaagat ctatttaggt ccacctgagg
     2041 aaagcgctgc accgccagtg cctccgcctc gggtgacagc aagaaggcac aaaccaatca
     2101 cgatttcaaa gcgcttgctg cgagaaagga cggttttcta tacttcttcc ctggatgaaa
     2161 gcgaagatga aatccaacat caaacaccga atggtactat caccagcagc atagaacccc
     2221 ccaagccacc acaacacccc aaactaccta ttcagaggag tggggaaact gatcctggga
     2281 ggaagtcccc aagcaggcct attttggatg gcaagttgga gccctgccca gaggtggacg
     2341 tggggaagtt ggtgtctagg ctgcaggatg gagggaccaa gatcacccca aaggccacca
     2401 atggacccat gccaggctct gggcccacca agaccccctc tttccacata aagagaccag
     2461 ctccccggcc cctggcccac cacaaggagg gggatgctga cagtttcagc aaagtgcggc
     2521 ctccaggaga aaagccaacc atcatccgcc ccccagtgag gcccccagat cctccctgcc
     2581 gggcagctac tccccaaaag ccagaaccaa agccagatat tgtggctggc aatgcggggg
     2641 aaatcacatc atctgtggtg gcttccagga ccaggttttt tgaaacagct tcccggaaaa
     2701 caggaagttc tcaaggcaga cttcctggag atgaaagttg aggctacagg ttttaaaagc
     2761 cttggcctca gaggaccctt tccaggttct gaaagagtct ttcttcttca aacctttgtg
     2821 tgcggagtca ttttgtgttg aagagcagct ccttcctagc cttgcacttt cagactctct
     2881 ctgggaggcc ataaaataag gagcatatgt cctagacagg tgtttatatc tcctttgtat
     2941 tctgtcttca tcccctcaga aggtctgttt tgagttccta taacactgtg aagagctgga
     3001 ctccctcaag ccagactctg ccaaaaccaa gatatccact tacctgagtt gaagagggga
     3061 gctcagtttt caactcttcc ctgaacttcc tgcttcctca gagggccatt gaactctgag
     3121 agatttgggg ctaaagactg atctcagggg tcttaccttg aactgaaggc cacttgagtt
     3181 ggggccattg cttaccttgg ttggaaggga atagaaatgt ttgctgaaca ttggagaatc
     3241 tcaacatgtc tcctactgag gatatggaca ctggtgccat gtcagcgctc tggtgctgca
     3301 gtatgttgcc aagagcccgt ctgctcctgc gaggctatga gtgggatgag tgatgccccc
     3361 acagcacctc catgtggact taggaaggtg gccttcctgc tgttacatgc agccacttag
     3421 gacaaatctg caaagcatgt tttgcatgta aaagcctagg tctatttgga ttattctttc
     3481 tccttttttt tgacagcttc ctgtcaagca atcaagaaac aaacaaaagc tgaacacatt
     3541 tctttttaaa aaaaggagac tgtgtttggt cctgtaggag ttctattttg gggtcaaatg
     3601 ctagaaaaat tgttaaggtg gattgaggcc aggcagctgt cactgctgct tcatgtttgc
     3661 cttctgcaca taaactcttt tatctcctga aaaaagcagt tcttaaccca gtgtccatgg
     3721 actcagaaac tccatgatgc ccctgagatg gtatgcacaa ttccatgaca atatgccctt
     3781 tctggggaga tagtccataa tgttctgcta aatttcaaat gggctcgtga cccaaaaaag
     3841 tcaagaacca cagcacttga gtaaaatact ctttttacaa tccatataag cccttgattg
     3901 gaagggcttt tcaaaatcat ttagtctaac aactgcccag tttccagtcg ggggaactga
     3961 ggcagagcaa ggtagtgatc acaccagtac aagatctcag gtcccaggct cctatgcaag
     4021 ttttttttcc ccattatatc acacttatct agcaagggac cttgtggttt gtggctttag
     4081 tggccatcat ttctgggggt tggcttttac cctttttctt gaatatttgc caccaagtga
     4141 aaaatgttag gacataaacc cttgccaggt ccctttcatt tgctatctct attattggaa
     4201 aggacctaaa aattggtgta atggggcaga aatctgagga atggacattt ctaattcctg
     4261 tttgttgaag ggaagttgct ggaaagagca tcagtacttg tttctatgca gatgcctggg
     4321 ccgtagcttg tctgtagcgt ctgtataatt ataatgttgc ccagtgtgag ggaaagagct
     4381 ttcctacttg cactcttcta ccaaggccct gttagtgcac tgattatagt actgacagat
     4441 aaacctagat aaagagatag agagtgagta catgcacact catgtgcaaa cccactcaga
     4501 gatgcatttg gaacagtgct actgaaaggc agtagtcatt ttcaagactg aattccaaac
     4561 atggtttatt ggtgagttag gaacatgtaa ggccaagtac actgagagcc tttttgaaag
     4621 taattgagtg gaaacttgat gccattctaa atcaaggcat atccaggtgg cccggttgaa
     4681 ctcactccac tgtacccagt ctcaaaggcc aggttgctaa gaaaccagga gtaaaagagt
     4741 caagtgacca tcatttcacc tgctgcttgc ccccaatagt agtctctgtg aggccttact
     4801 gacctcacct aggaagtgat ttttgagccc ttgtttcagg gctgtggcct ccctgctcta
     4861 tcctgaataa agcagacagg tgtgcagatt ttggccatga aagcatggct aatagggcca
     4921 cagtcccttt aaagaaacat ggtttgactc tggttttctt gggggaaaat accacaatca
     4981 ccgatgcaaa cattggaaga ttattgagag ccctagaaag ctgctgtgat cccagtagaa
     5041 aatatgtccc agaaatgtca tgagattgct gtgtgttgcc tgggacacag atcaagggcc
     5101 tatcttggag agctggggac cagcagtctg cctggaggcc agggagcagt ggctgagtag
     5161 ctctgccttt gctctggtct ataccttaag aatgccaaag aatgaatttc aacgcctgcc
     5221 tttggcactc tgacttaaag tgcaaaaagc ttctgtggcg aggcatgcta tcatggaatg
     5281 agactggctt gccctaggct taatggatgg gcagtcattt tgcagaggct atgggaagag
     5341 ggtgataata gaagagtggc agctatagga aattatcaac ataccttggc cagcaagtta
     5401 gagaatctgg caatggatga actgaaagtg atgaactggc agggataaca aagaacctaa
     5461 catttattaa gcacgtattt attaactgct cagtgtttca tattcatgca agtattctca
     5521 ttttacagag aaagaaatta tggcccaggg ggctaaagta aacaactcaa gggcacatag
     5581 aaagtaaata aaaggactgt gatttgaatc caggccactc ttagcccatg ctgttttccc
     5641 tttgccacac tgtggtaggt gtttgaacag aggccacatt actagagttg gcatgactct
     5701 tgactcttgc ctgcctaaca aaatattgaa aggcaaacat ttgaaggagg gagggggtgc
     5761 aggttcagtt tatatggaaa tgcaaactgg gctggaagat attcctgagt taggctttct
     5821 cttcatattc agcttgcaca tttggtaatg tttttaagat gatcatctaa ttttattttg
     5881 tgaagtgaag gatttgtgtt ttagttggca gttgttaagt ccttggcttg ccatttttca
     5941 aaaagtaaaa aggtcctcac aggtgtctcc atacttcgcc aaggttgtag catgggcagt
     6001 ttcagtttca gcctaagaga ctggtgacat ccacaaatgc agttttagaa gcagaaaagg
     6061 tcttggtgcc tctgcagtac ttgatgtatt ggggtcaaat ctctacaaat ttttctgtgg
     6121 tgatagcaaa atcaagagat ggcttacaaa aagaaatatt gaatttttat ttttgaagtt
     6181 tttgtttttt aaaaggttgg gggtgttcag ccactgaggg acaaaactta gcatctaatt
     6241 tcaattatag tgtcatgcag agtatttcta aagtaattgg ttatcatggg aaagtattct
     6301 cttttcaaga agttctttga ttctgtaata actagaacaa ataaagtagt aaaagaagaa
     6361 atagttctgt gactaggaaa aaattgcttt tgagagaaca tagatcaatt atactacttc
     6421 taaggtagct gcagataagt ggccttgaca cattacaagc ctggaaaaaa acatcagaaa
     6481 taataaaaaa tttcagagag aatcaagata cctttttttt tctttttttt ttcttttttt
     6541 tattatactc taagttttag ggtacatgtg cacattgtgc aggttagtta catatgtata
     6601 catgtgccat gctggtgcgc tgcacccact aatgtgtcat ctagcattag gtatatctcc
     6661 cagtgctatc cctcccccct cccccgaccc caccacagtc cccagagtgt gatattcccc
     6721 ttcctgtgtc catgtgatct cattgttcaa ttcccaccta tgagtgagaa tatgcggtgt
     6781 ttggtttttt gttcttgcga tagtttactg agaatgatgg tttccaattt catccatgtc
     6841 cctacaaagg atatgaactc atcatttttt atggctgcat agtattccat ggtgtatatg
     6901 tgccacattt tcttaatcca gtctatcatt gttggacatt tgggttggtt ccaagtcttt
     6961 gctattgtga atagtgccgc aataaacata cgtgtgcatg tgtctttata gcagcatgat
     7021 ttatactcat ttgggtatat acccagtaat gggatggctg ggtcaaatgg tatttctagt
     7081 tctagatccc tgaggaatcg ccacactgac ttccacaatg gttgaactag tttacagtcc
     7141 aaccaacagt gtaaaagtgt tcctatttct ccgcatcctc tccagcacct gttgtttcct
     7201 gactttttaa tgattgccat tctaactggt gtgagatgat atctcatagt ggttttgatt
     7261 tgcatttctc tgatggccag tgatgatgag catttcttca tgtgtttttt ggctgcataa
     7321 atgtcttctt ttgagaagtg tctgttcatg tccttcgcga aggacaagat accttaaaga
     7381 taagttttcg ataaagaaaa gccttcattt tcctggggga aatgaagcac ttgatgccaa
     7441 ggacattagt agagggctag aaagattgcc tgtgtgaaag acatatgcta tatccaaata
     7501 ataaaacaaa cgagatcctg gggctcattc atgtgacttt caataaagta tgtagactgt
     7561 ctaatataa
//
