LOCUS       NM_130837               6429 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens OPA1 mitochondrial dynamin like GTPase (OPA1),
            transcript variant 8, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_130837
VERSION     NM_130837.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   de Muijnck,C., Haer-Wigman,L., van Everdingen,J.A.M., Lushchyk,T.,
            Heutinck,P.A.T., van Dooren,M.F., Kievit,A.J.A., Verhoeven,V.J.M.,
            Simon,M.E.H., Wasmann,R.A., Notting,I.C., De Baere,E., Walraedt,S.,
            De Zaeytijd,J., Van den Broeck,F., Leroy,B.P., Boon,C.J.F. and van
            Genderen,M.M.
  TITLE     Characteristics of autosomal dominant WFS1-associated optic
            neuropathy and its comparability to OPA1-associated autosomal
            dominant optic atrophy
  JOURNAL   Sci Rep 14 (1), 22956 (2024)
   PUBMED   39363032
  REMARK    GeneRIF: Characteristics of autosomal dominant WFS1-associated
            optic neuropathy and its comparability to OPA1-associated autosomal
            dominant optic atrophy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Nitta,Y., Osaka,J., Maki,R., Hakeda-Suzuki,S., Suzuki,E., Ueki,S.,
            Suzuki,T. and Sugie,A.
  TITLE     Drosophila model to clarify the pathological significance of OPA1
            in autosomal dominant optic atrophy
  JOURNAL   Elife 12, 87880 (2024)
   PUBMED   39177028
  REMARK    GeneRIF: Drosophila model to clarify the pathological significance
            of OPA1 in autosomal dominant optic atrophy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6429)
  AUTHORS   Liang,F.G., Zandkarimi,F., Lee,J., Axelrod,J.L., Pekson,R.,
            Yoon,Y., Stockwell,B.R. and Kitsis,R.N.
  TITLE     OPA1 promotes ferroptosis by augmenting mitochondrial ROS and
            suppressing an integrated stress response
  JOURNAL   Mol Cell 84 (16), 3098-3114 (2024)
   PUBMED   39142278
  REMARK    GeneRIF: OPA1 promotes ferroptosis by augmenting mitochondrial ROS
            and suppressing an integrated stress response.
REFERENCE   4  (bases 1 to 6429)
  AUTHORS   Wei,Y.Y., Ye,J.J., Zhang,D.W., Hu,L., Wu,H.M. and Fei,G.H.
  TITLE     Melatonin Rescues Influenza A Virus-Induced Cellular Energy
            Exhaustion via OMA1-OPA1-S in Acute Exacerbation of COPD
  JOURNAL   J Pineal Res 76 (5), e12991 (2024)
   PUBMED   39039850
  REMARK    GeneRIF: Melatonin Rescues Influenza A Virus-Induced Cellular
            Energy Exhaustion via OMA1-OPA1-S in Acute Exacerbation of COPD.
REFERENCE   5  (bases 1 to 6429)
  AUTHORS   Delettre,C., Griffoin,J.M., Kaplan,J., Dollfus,H., Lorenz,B.,
            Faivre,L., Lenaers,G., Belenguer,P. and Hamel,C.P.
  TITLE     Mutation spectrum and splicing variants in the OPA1 gene
  JOURNAL   Hum Genet 109 (6), 584-591 (2001)
   PUBMED   11810270
REFERENCE   6  (bases 1 to 6429)
  AUTHORS   Alexander,C., Votruba,M., Pesch,U.E., Thiselton,D.L., Mayer,S.,
            Moore,A., Rodriguez,M., Kellner,U., Leo-Kottler,B., Auburger,G.,
            Bhattacharya,S.S. and Wissinger,B.
  TITLE     OPA1, encoding a dynamin-related GTPase, is mutated in autosomal
            dominant optic atrophy linked to chromosome 3q28
  JOURNAL   Nat Genet 26 (2), 211-215 (2000)
   PUBMED   11017080
REFERENCE   7  (bases 1 to 6429)
  AUTHORS   Delettre,C., Lenaers,G., Griffoin,J.M., Gigarel,N., Lorenzo,C.,
            Belenguer,P., Pelloquin,L., Grosgeorge,J., Turc-Carel,C.,
            Perret,E., Astarie-Dequeker,C., Lasquellec,L., Arnaud,B.,
            Ducommun,B., Kaplan,J. and Hamel,C.P.
  TITLE     Nuclear gene OPA1, encoding a mitochondrial dynamin-related
            protein, is mutated in dominant optic atrophy
  JOURNAL   Nat Genet 26 (2), 207-210 (2000)
   PUBMED   11017079
REFERENCE   8  (bases 1 to 6429)
  AUTHORS   Johnston,R.L., Seller,M.J., Behnam,J.T., Burdon,M.A. and
            Spalton,D.J.
  TITLE     Dominant optic atrophy. Refining the clinical diagnostic criteria
            in light of genetic linkage studies
  JOURNAL   Ophthalmology 106 (1), 123-128 (1999)
   PUBMED   9917792
REFERENCE   9  (bases 1 to 6429)
  AUTHORS   Votruba,M., Moore,A.T. and Bhattacharya,S.S.
  TITLE     Demonstration of a founder effect and fine mapping of dominant
            optic atrophy locus on 3q28-qter by linkage disequilibrium method:
            a study of 38 British Isles pedigrees
  JOURNAL   Hum Genet 102 (1), 79-86 (1998)
   PUBMED   9490303
REFERENCE   10 (bases 1 to 6429)
  AUTHORS   Delettre-Cribaillet,C., Hamel,C.P. and Lenaers,G.
  TITLE     Optic Atrophy Type 1 - RETIRED CHAPTER, FOR HISTORICAL REFERENCE
            ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301426
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC048351.18 and AC106710.8.
            
            On Mar 25, 2020 this sequence version replaced NM_130837.2.
            
            Summary: The protein encoded by this gene is a nuclear-encoded
            mitochondrial protein with similarity to dynamin-related GTPases.
            The encoded protein localizes to the inner mitochondrial membrane
            and helps regulate mitochondrial stability and energy output. This
            protein also sequesters cytochrome c. Mutations in this gene have
            been associated with optic atrophy type 1, which is a dominantly
            inherited optic neuropathy resulting in progressive loss of visual
            acuity, leading in many cases to legal blindness. [provided by
            RefSeq, Aug 2017].
            
            Transcript Variant: This variant (8) contains 2 additional exons,
            4b and 5b, compared to transcript variant 1. It however, maintains
            the same reading frame and encodes an isoform (8) of 1015 aa. This
            variant is based on an alternate splice pattern characterized by
            Delettre et al (2001, PMID: 11810270), but the complete 5' to 3'
            exon combination is inferred and not supported at the time of
            review by a single long cDNA.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.543005.1,
                                           SRR18074967.3232494.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000361510.8/
                                                  ENSP00000355324.2
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-202               AC048351.18        73764-73965
            203-521             AC048351.18        95279-95597
            522-618             AC048351.18        96230-96326
            619-726             AC048351.18        97734-97841
            727-780             AC048351.18        98340-98393
            781-848             AC048351.18        99425-99492
            849-959             AC048351.18        106648-106758
            960-1013            AC048351.18        112168-112221
            1014-1118           AC048351.18        115974-116078
            1119-1205           AC048351.18        117751-117837
            1206-1319           AC048351.18        118508-118621
            1320-1400           AC048351.18        123321-123401
            1401-1475           AC048351.18        123531-123605
            1476-1547           AC048351.18        123929-124000
            1548-1647           AC048351.18        124084-124183
            1648-1778           AC048351.18        124531-124661
            1779-1851           AC048351.18        126109-126181
            1852-1924           AC048351.18        126284-126356
            1925-2040           AC048351.18        127621-127736
            2041-2105           AC048351.18        128626-128690
            2106-2182           AC048351.18        129351-129427
            2183-2348           AC048351.18        135418-135583
            2349-2501           AC048351.18        137636-137788
            2502-2610           AC048351.18        139443-139551
            2611-2690           AC048351.18        140038-140117
            2691-2831           AC048351.18        143378-143518
            2832-2948           AC048351.18        145436-145552
            2949-3042           AC048351.18        146852-146945
            3043-3153           AC048351.18        147726-147836
            3154-3223           AC106710.8         12971-13040
            3224-6429           AC106710.8         15514-18719
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..6429
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /note="OPA1 mitochondrial dynamin like GTPase"
                     /db_xref="GeneID:4976"
                     /db_xref="HGNC:HGNC:8140"
                     /db_xref="MIM:605290"
     exon            1..202
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..3218
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /EC_number="3.6.5.5"
                     /note="isoform 8 is encoded by transcript variant 8;
                     mitochondrial dynamin-like GTPase; dynamin-like 120 kDa
                     protein, mitochondrial; optic atrophy protein 1;
                     dynamin-like guanosine triphosphatase; optic atrophy 1
                     (autosomal dominant); dynamin-like GTPase OPA1,
                     mitochondrial"
                     /codon_start=1
                     /product="dynamin-like GTPase OPA1, mitochondrial isoform
                     8"
                     /protein_id="NP_570850.2"
                     /db_xref="GeneID:4976"
                     /db_xref="HGNC:HGNC:8140"
                     /db_xref="MIM:605290"
                     /translation="MWRLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHLVSRSIYHSH
                     HPTLKLQRPQLRTSFQQFSSLTNLPLRKLKFSPIKYGYQPRRNFWPARLATRLLKLRY
                     LILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWIVPDIVWEIDEYIDFEKIRKALPSSE
                     DLVKLAPDFDKIVESLSLLKDFFTSGHKLVSEVIGASDLLLLLGSPEETAFRATDRGS
                     ESDKHFRKGLLGELILLQQQIQEHEEEARRAAGQYSTSYAQQKRKVSDKEKIDQLQEE
                     LLHTQLKYQRILERLEKENKELRKLVLQKDDKGIHHRKLKKSLIDMYSEVLDVLSDYD
                     ASYNTQDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHH
                     VALFKDSSREFDLTKEEDLAALRHEIELRMRKNVKEGCTVSPETISLNVKGPGLQRMV
                     LVDLPGVINTVTSGMAPDTKETIFSISKAYMQNPNAIILCIQDGSVDAERSIVTDLVS
                     QMDPHGRRTIFVLTKVDLAEKNVASPSRIQQIIEGKLFPMKALGYFAVVTGKGNSSES
                     IEAIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSDCFWKMVRESVEQQADSFKA
                     TRFNLETEWKNNYPRLRELDRNELFEKAKNEILDEVISLSQVTPKHWEEILQQSLWER
                     VSTHVIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSRFM
                     TEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDA
                     AIYFMEEALQARLKDTENAIENMVGPDWKKRWLYWKNRTQEQCVHNETKNELEKMLKC
                     NEEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTALNHCNLCRRG
                     FYYYQRHFVDSELECNDVVLFWRIQRMLAITANTLRQQLTNTEVRRLEKNVKEVLEDF
                     AEDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK"
     exon            203..521
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            522..618
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            619..726
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            727..780
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            781..848
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            849..959
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1013
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1118
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1205
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1319
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1400
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1475
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..1547
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1647
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            1648..1778
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1851
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            1852..1924
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            1925..2040
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            2041..2105
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            2106..2182
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            2183..2348
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            2349..2501
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..2610
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            2611..2690
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            2691..2831
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            2832..2948
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            2949..3042
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            3043..3153
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            3154..3223
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     exon            3224..6429
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3326..3331
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /note="hexamer: AATAAA"
     polyA_site      3349
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /note="major polyA site"
     regulatory      5224..5229
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /note="hexamer: AATAAA"
     polyA_site      5248
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
     regulatory      6124..6129
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /note="hexamer: ATTAAA"
     polyA_site      6145
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /note="major polyA site"
     regulatory      6406..6411
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /note="hexamer: TATAAA"
     polyA_site      6429
                     /gene="OPA1"
                     /gene_synonym="BERHS; largeG; MGM1; MTDPS14; NPG; NTG"
                     /note="major polyA site"
ORIGIN      
        1 aggctcttgc ggaagtccat gcgccattgg gagggcctcg gccgcggctc tgtgcccttg
       61 ctgctgaggg ccacttcctg ggtcattcct ggaccgggag ccgggctggg gctcacacgg
      121 gggctcccgc gtggccgtct cggcgcctgc gtgacctccc cgccggcggg atgtggcgac
      181 tacgtcgggc cgctgtggcc tgtgaggtct gccagtcttt agtgaaacac agctctggaa
      241 taaaaggaag tttaccacta caaaaactac atctggtttc acgaagcatt tatcattcac
      301 atcatcctac cttaaagctt caacgacccc aattaaggac atcctttcag cagttctctt
      361 ctctgacaaa ccttccttta cgtaaactga aattctctcc aattaaatat ggctaccagc
      421 ctcgcaggaa tttttggcca gcaagattag ctacgagact cttaaaactt cgctatctca
      481 tactaggatc ggctgttggg ggtggctaca cagccaaaaa gacttttgat cagtggaaag
      541 atatgatacc ggaccttagt gaatataaat ggattgtgcc tgacattgtg tgggaaattg
      601 atgagtatat cgattttgag aaaattagaa aagcccttcc tagttcagaa gaccttgtaa
      661 agttagcacc agactttgac aagattgttg aaagccttag cttattgaag gactttttta
      721 cctcaggtca caaattggtt agtgaagtca taggagcttc tgacctactt ctcttgttag
      781 gttctccgga agaaacggcg tttagagcaa cagatcgtgg atctgaaagt gacaagcatt
      841 ttagaaaggg tctgcttggt gagctcattc tcttacaaca acaaattcaa gagcatgaag
      901 aggaagcgcg cagagccgct ggccaatata gcacgagcta tgcccaacag aagcgcaagg
      961 tgtcagacaa agagaaaatt gaccaacttc aggaagaact tctgcacact cagttgaagt
     1021 atcagagaat cttggaacga ttagaaaagg agaacaaaga attgagaaaa ttagtattgc
     1081 agaaagatga caaaggcatt catcatagaa agcttaagaa atctttgatt gacatgtatt
     1141 ctgaagttct tgatgttctc tctgattatg atgccagtta taatacgcaa gatcatctgc
     1201 cacgggttgt tgtggttgga gatcagagtg ctggaaagac tagtgtgttg gaaatgattg
     1261 cccaagctcg aatattccca agaggatctg gggagatgat gacacgttct ccagttaagg
     1321 tgactctgag tgaaggtcct caccatgtgg ccctatttaa agatagttct cgggagtttg
     1381 atcttaccaa agaagaagat cttgcagcat taagacatga aatagaactt cgaatgagga
     1441 aaaatgtgaa agaaggctgt accgttagcc ctgagaccat atccttaaat gtaaaaggcc
     1501 ctggactaca gaggatggtg cttgttgact taccaggtgt gattaatact gtgacatcag
     1561 gcatggctcc tgacacaaag gaaactattt tcagtatcag caaagcttac atgcagaatc
     1621 ctaatgccat catactgtgt attcaagatg gatctgtgga tgctgaacgc agtattgtta
     1681 cagacttggt cagtcaaatg gaccctcatg gaaggagaac catattcgtt ttgaccaaag
     1741 tagacctggc agagaaaaat gtagccagtc caagcaggat tcagcagata attgaaggaa
     1801 agctcttccc aatgaaagct ttaggttatt ttgctgttgt aacaggaaaa gggaacagct
     1861 ctgaaagcat tgaagctata agagaatatg aagaagagtt ttttcagaat tcaaagctcc
     1921 taaagacaag catgctaaag gcacaccaag tgactacaag aaatttaagc cttgcagtat
     1981 cagactgctt ttggaaaatg gtacgagagt ctgttgaaca acaggctgat agtttcaaag
     2041 caacacgttt taaccttgaa actgaatgga agaataacta tcctcgcctg cgggaacttg
     2101 accggaatga actatttgaa aaagctaaaa atgaaatcct tgatgaagtt atcagtctga
     2161 gccaggttac accaaaacat tgggaggaaa tccttcaaca atctttgtgg gaaagagtat
     2221 caactcatgt gattgaaaac atctaccttc cagctgcgca gaccatgaat tcaggaactt
     2281 ttaacaccac agtggatatc aagcttaaac agtggactga taaacaactt cctaataaag
     2341 cagtagaggt tgcttgggag accctacaag aagaattttc ccgctttatg acagaaccga
     2401 aagggaaaga gcatgatgac atatttgata aacttaaaga ggctgttaag gaagaaagta
     2461 ttaaacgaca caagtggaat gactttgcgg aggacagctt gagggttatt caacacaatg
     2521 ctttggaaga ccgatccata tctgataaac agcaatggga tgcagctatt tattttatgg
     2581 aagaggctct gcaggctcgt ctcaaggata ctgaaaatgc aattgaaaac atggtgggtc
     2641 cagactggaa aaagaggtgg ttatactgga agaatcggac ccaagaacag tgtgttcaca
     2701 atgaaaccaa gaatgaattg gagaagatgt tgaaatgtaa tgaggagcac ccagcttatc
     2761 ttgcaagtga tgaaataacc acagtccgga agaaccttga atcccgagga gtagaagtag
     2821 atccaagctt gattaaggat acttggcatc aagtttatag aagacatttt ttaaaaacag
     2881 ctctaaacca ttgtaacctt tgtcgaagag gtttttatta ctaccaaagg cattttgtag
     2941 attctgagtt ggaatgcaat gatgtggtct tgttttggcg tatacagcgc atgcttgcta
     3001 tcaccgcaaa tactttaagg caacaactta caaatactga agttaggcga ttagagaaaa
     3061 atgttaaaga ggtattggaa gattttgctg aagatggtga gaagaagatt aaattgctta
     3121 ctggtaaacg cgttcaactg gcggaagacc tcaagaaagt tagagaaatt caagaaaaac
     3181 ttgatgcttt cattgaagct cttcatcagg agaaataaat taaaatcgta ctcataatca
     3241 gctctgcata catctgaaga acaaaaacat caacgtcttt tgtccagcct ctttttcttc
     3301 tgctgttcca cctttctaaa catacaataa agtcatggga taaaaataat cgatgtatgt
     3361 tacgggcgct ttaaccatca gctgcctctc gaatggaaga acagtggtaa tggattaaca
     3421 tcctattttg ttgtactaaa gtgacaaatc ggaataatat aattggtatg gccattaggt
     3481 tcagtccttg aagataagaa acttgttctc tgtttgttgt cttatttgtg gtggcactcg
     3541 tttaatggat taactgaggt tgctcaatgt tcagtttctt ttccagaaat acaatgctag
     3601 gtgttttgaa ataaaactta tatagcaatt gtttaaagtt atcaattgta tataaaatca
     3661 cagtagcctg ctaaatcatt gtatgtgtct gtagtattct attcccagaa actatttgac
     3721 catgataatt cagtttatat tcaccacatg aaagaaaaat gggtaacaga agaaccctta
     3781 aaacaggtta atttggattg taacgttcag tgaaagaaat ttcaaccctt catagccagc
     3841 gaagaaattt gccttggaag ccaagtcagt accagcttac ctatttgatt cagttgctgt
     3901 tttctcactc tctatatcca tttgaaattg atttatttta gatgttgtat acttacgtta
     3961 ggctttctgt taatagtggt ttttctcctg ttgacagagc caccggatta tgacacagga
     4021 tgaggaagat taaggataat caattgacta atttcattta gaatattatc aaacatttca
     4081 actaggtatc agaaaaaggc tttctttcat aagactattt taaatagaaa ttatttcaac
     4141 aattaaagta atgttgacca tccccctctc agctgaataa agaaaaattt agttcaattt
     4201 attgcaattt aattacaata ctaccttcac aacattttca tgtgttttaa ataaatattt
     4261 tttaattggc taaaggacat tcaagcaaag aaatgctttc tttacttaaa atgtctatct
     4321 catttgctgc cttttcacta agcctttact ttgttaataa aagtgtccat tgtgtgatgt
     4381 ttttgatttt acagtttgct aaatcttatt ttcttggagt tgctttttgg taacagcccc
     4441 attgctactc cccattttat tgttttacat caatgcatgc ttcgttgtga tccctcaaga
     4501 tgtaacactt ggtatgctcg gttgaggata tgaaaaaata cttccgaaac caggaattca
     4561 atgtatgttt gttttatact gtttgataag aaaagtaggt ccagccttaa gcagcacaga
     4621 tgcgctggta gatgcatagt caggaacttt ttttatttct tttaggtcta gggacaggag
     4681 tgaatagaaa gggaggagag ctctattatg ttctatacac agattaggag atgaccttac
     4741 tgggtacacc cctctaacca gtgcttacag gttaatgcat gttaatgaat atttttgcag
     4801 ttgtaaagca taacaattac aactacacat ctatttctaa agaataaaac aggaccatat
     4861 ttatttactt ctgtcaacta tagaaagaaa gaccttcagc tgtatttcca cagatttctc
     4921 ccaaggaaaa ggctaatatt agtcactact gttatcacat ccctttgtat aagttttaaa
     4981 aagagatgga gggagatctt catttctttg aggagatcag tattgtaacg tatgtgaata
     5041 gatgataaca attaatatta ctaaaagtcc cacatgagag tcctgacgcc ctctccatgc
     5101 cccacagtaa tgtggcttct ttcatgggtt tttttttctt ctttttagct gatctcatcc
     5161 taagcatgct ttatttttcc ttgaaagcta ggtatttatc aactgcagat gttattgaaa
     5221 gaaaataaaa ttcagtctca agagtaaacc ctgtgtcttg tgtctgtagt tcaaaagtca
     5281 gaaatgattc taatttaaac aaaaagatac taaatataca gaagttaaat tcgaactagc
     5341 cacagaatca tttgttttta tgtcagaatt tgcaaagagt ggagtggaca aagctctgta
     5401 tggaagactg aacaactgta aatagatgat atccaaactt aatttggcta ggacttcaat
     5461 tttaaaaatc agtgtaccta ggcagtgcac agcacgaaat aagtggccct tgcagcttcc
     5521 ccgtttaacc cactgtgcta tagttgcggg tggaacagtc aacctttcta gtagtttatg
     5581 atattgccct ctttgtattc ccattttcta cagttttttc cgcagacttc tttctgcaaa
     5641 ttattcagcc tccaaatgca aatgaatgat ataaaaataa gtagggaaca tggcagagag
     5701 tggtgcttcc cagcctcaca atgtgggaat ttgacatagg atgagagtca gagtataggt
     5761 ttaaaagata aaatctttag ttaataattt tgtatttatt tattctagat gtatgtatct
     5821 gaggaaagaa atctggtatt tttgctttcc aataaagggg atcaaagtaa tggtttttct
     5881 ctcagttctc taagctggtc tatgttatag ctctagcagt atggaaatgt gctttaaaat
     5941 atgcttacct tttgaatgat catggctata tgttgttgag atatttgaaa cttaccttgt
     6001 tttcacttgt gcactgtgaa tgaactttgt attatttttt taaaaccttc acattacgtg
     6061 tagatattat tgcaacttat attttgcctg agcttgatca aaggtcattt gtgtagatga
     6121 gtaattaaaa aatatttaaa tcacattata attctattat tggagagcat cttttaaatt
     6181 tttttctgtt ttaacgaggg aaagagaaac ctgtatacct agggtcatta tttgacccca
     6241 tagtataacc agattcatgg tctaacaagc tctcagtgtg gcttttctct gaatgcttga
     6301 atttcacatg ccttgcattt cacagttgta ctccatggtc aaccggtgct ttttttcaca
     6361 tcgtggtact tgtcaaaaca ttttgttatt ttccttggta aaatatataa aaaaggtttt
     6421 ctaatttca
//
