LOCUS       NM_004852              16433 bp    mRNA    linear   PRI 12-AUG-2024
DEFINITION  Homo sapiens one cut homeobox 2 (ONECUT2), mRNA.
ACCESSION   NM_004852
VERSION     NM_004852.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 16433)
  AUTHORS   Lin,M., Tu,R.H., Wu,S.Z., Zhong,Q., Weng,K., Wu,Y.K., Lin,G.T.,
            Wang,J.B., Zheng,C.H., Xie,J.W., Lin,J.X., Chen,Q.Y., Huang,C.M.,
            Cao,L.L. and Li,P.
  TITLE     Increased ONECUT2 induced by Helicobacter pylori promotes gastric
            cancer cell stemness via an AKT-related pathway
  JOURNAL   Cell Death Dis 15 (7), 497 (2024)
   PUBMED   38997271
  REMARK    GeneRIF: Increased ONECUT2 induced by Helicobacter pylori promotes
            gastric cancer cell stemness via an AKT-related pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16433)
  AUTHORS   Li,K., Zhou,M. and Zhang,Y.
  TITLE     MicroRNA-1298-3p induces tumor-suppressive effects in human
            cervical cancer cells via post-transcriptional suppression of
            ONECUT2
  JOURNAL   Acta Biochim Pol 69 (4), 737-743 (2022)
   PUBMED   36508388
  REMARK    GeneRIF: MicroRNA-1298-3p induces tumor-suppressive effects in
            human cervical cancer cells via post-transcriptional suppression of
            ONECUT2.
REFERENCE   3  (bases 1 to 16433)
  AUTHORS   Yi,Y., Yang,N., Yang,Z., Tao,X. and Li,Y.
  TITLE     LncRNA TM1-3P Regulates Proliferation, Apoptosis and Inflammation
            of Fibroblasts in Osteoarthritis through miR-144-3p/ONECUT2 Axis
  JOURNAL   Orthop Surg 14 (11), 3078-3091 (2022)
   PUBMED   36178080
  REMARK    GeneRIF: LncRNA TM1-3P Regulates Proliferation, Apoptosis and
            Inflammation of Fibroblasts in Osteoarthritis through
            miR-144-3p/ONECUT2 Axis.
REFERENCE   4  (bases 1 to 16433)
  AUTHORS   Shen,C., Wang,J., Xu,Z., Zhang,L., Gu,W. and Zhou,X.
  TITLE     ONECUT2 which is targeted by hsa-miR-15a-5p enhances stemness
            maintenance of gastric cancer stem cells
  JOURNAL   Exp Biol Med (Maywood) 246 (24), 2645-2659 (2021)
   PUBMED   34365839
  REMARK    GeneRIF: ONECUT2 which is targeted by hsa-miR-15a-5p enhances
            stemness maintenance of gastric cancer stem cells.
REFERENCE   5  (bases 1 to 16433)
  AUTHORS   Wang,G.H., Zhou,Y.M., Yu,Z., Deng,J.P., Liu,S.F., Wei,C.Z.,
            Feng,Y., Mao,M. and Wang,Z.
  TITLE     Up-regulated ONECUT2 and down-regulated SST promote gastric cell
            migration, invasion, epithelial-mesenchymal transition and tumor
            growth in gastric cancer
  JOURNAL   Eur Rev Med Pharmacol Sci 24 (18), 9378-9390 (2020)
   PUBMED   33015779
  REMARK    GeneRIF: Up-regulated ONECUT2 and down-regulated SST promote
            gastric cell migration, invasion, epithelial-mesenchymal transition
            and tumor growth in gastric cancer.
REFERENCE   6  (bases 1 to 16433)
  AUTHORS   Furuno,K., Ikeda,K., Hamano,S., Fukuyama,K., Sonoda,M., Hara,T.,
            Sasazuki,T. and Yamamoto,K.
  TITLE     Onecut transcription factor OC2 is a direct target of T-bet in
            type-1 T-helper cells
  JOURNAL   Genes Immun 9 (4), 302-308 (2008)
   PUBMED   18418398
  REMARK    GeneRIF: OC2 transcription factor is a direct target of T-bet in
            type-1 T-helper cells.
REFERENCE   7  (bases 1 to 16433)
  AUTHORS   Pike,B.L., Greiner,T.C., Wang,X., Weisenburger,D.D., Hsu,Y.H.,
            Renaud,G., Wolfsberg,T.G., Kim,M., Weisenberger,D.J.,
            Siegmund,K.D., Ye,W., Groshen,S., Mehrian-Shai,R., Delabie,J.,
            Chan,W.C., Laird,P.W. and Hacia,J.G.
  TITLE     DNA methylation profiles in diffuse large B-cell lymphoma and their
            relationship to gene expression status
  JOURNAL   Leukemia 22 (5), 1035-1043 (2008)
   PUBMED   18288132
  REMARK    GeneRIF: A CpG island proximal to ONECUT2 is frequently methylated
            in diffuse large B-cell lymphoma (DLBCL).
REFERENCE   8  (bases 1 to 16433)
  AUTHORS   Rauch,T., Li,H., Wu,X. and Pfeifer,G.P.
  TITLE     MIRA-assisted microarray analysis, a new technology for the
            determination of DNA methylation patterns, identifies frequent
            methylation of homeodomain-containing genes in lung cancer cells
  JOURNAL   Cancer Res 66 (16), 7939-7947 (2006)
   PUBMED   16912168
REFERENCE   9  (bases 1 to 16433)
  AUTHORS   Jacquemin,P., Lannoy,V.J., O'Sullivan,J., Read,A., Lemaigre,F.P.
            and Rousseau,G.G.
  TITLE     The transcription factor onecut-2 controls the
            microphthalmia-associated transcription factor gene
  JOURNAL   Biochem Biophys Res Commun 285 (5), 1200-1205 (2001)
   PUBMED   11478782
REFERENCE   10 (bases 1 to 16433)
  AUTHORS   Jacquemin,P., Lannoy,V.J., Rousseau,G.G. and Lemaigre,F.P.
  TITLE     OC-2, a novel mammalian member of the ONECUT class of homeodomain
            transcription factors whose function in liver partially overlaps
            with that of hepatocyte nuclear factor-6
  JOURNAL   J Biol Chem 274 (5), 2665-2671 (1999)
   PUBMED   9915796
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC090340.6, Y18198.1 and
            BC013134.1.
            
            On Nov 22, 2018 this sequence version replaced NM_004852.2.
            
            Summary: This gene encodes a member of the onecut family of
            transcription factors, which are characterized by a cut domain and
            an atypical homeodomain. The protein binds to specific DNA
            sequences and stimulates expression of target genes, including
            genes involved in melanocyte and hepatocyte differentiation.
            [provided by RefSeq, Jul 2008].
            
            CCDS Note: This CCDS representation uses the 5'-most in-frame start
            codon, which is conserved in other vertebrates, including mouse,
            opossum, pig and zebrafish. An alternative downstream start codon,
            which has a stronger Kozak signal, also exists. It is possible that
            leaky scanning by ribosomes would allow the downstream start codon
            to be used, at least some of the time. The use of the downstream
            start codon would result in a protein that is 19 aa shorter at the
            N-terminus. There is no experimental evidence showing which start
            codon is preferentially used in vivo.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.3799795.1,
                                           SRR14038196.26159.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2144835
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000491143.3/ ENSP00000419185.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-311               AC090340.6         75192-75502         c
            312-320             Y18198.1           1-9
            321-567             AC090340.6         74936-75182         c
            568-1967            Y18198.1           256-1655
            1968-15892          AC090340.6         20119-34043         c
            15893-16433         BC013134.1         1081-1621
FEATURES             Location/Qualifiers
     source          1..16433
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.31"
     gene            1..16433
                     /gene="ONECUT2"
                     /gene_synonym="OC-2; OC2"
                     /note="one cut homeobox 2"
                     /db_xref="GeneID:9480"
                     /db_xref="HGNC:HGNC:8139"
                     /db_xref="MIM:604894"
     exon            1..1571
                     /gene="ONECUT2"
                     /gene_synonym="OC-2; OC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             344..1858
                     /gene="ONECUT2"
                     /gene_synonym="OC-2; OC2"
                     /note="onecut 2; ONECUT-2 homeodomain transcription
                     factor; HNF-6-beta; transcription factor ONECUT-2;
                     hepatocyte nuclear factor 6-beta"
                     /codon_start=1
                     /product="one cut domain family member 2"
                     /protein_id="NP_004843.2"
                     /db_xref="CCDS:CCDS42440.1"
                     /db_xref="GeneID:9480"
                     /db_xref="HGNC:HGNC:8139"
                     /db_xref="MIM:604894"
                     /translation="MKAAYTAYRCLTKDLEGCAMNPELTMESLGTLHGPAGGGSGGGG
                     GGGGGGGGGGPGHEQELLASPSPHHAGRGAAGSLRGPPPPPTAHQELGTAAAAAAAAS
                     RSAMVTSMASILDGGDYRPELSIPLHHAMSMSCDSSPPGMGMSNTYTTLTPLQPLPPI
                     STVSDKFHHPHPHHHPHHHHHHHHQRLSGNVSGSFTLMRDERGLPAMNNLYSPYKEMP
                     GMSQSLSPLAATPLGNGLGGLHNAQQSLPNYGPPGHDKMLSPNFDAHHTAMLTRGEQH
                     LSRGLGTPPAAMMSHLNGLHHPGHTQSHGPVLAPSRERPPSSSSGSQVATSGQLEEIN
                     TKEVAQRITAELKRYSIPQAIFAQRVLCRSQGTLSDLLRNPKPWSKLKSGRETFRRMW
                     KWLQEPEFQRMSALRLAACKRKEQEPNKDRNNSQKKSRLVFTDLQRRTLFAIFKENKR
                     PSKEMQITISQQLGLELTTVSNFFMNARRRSLEKWQDDLSTGGSSSTSSTCTKA"
     exon            1572..16433
                     /gene="ONECUT2"
                     /gene_synonym="OC-2; OC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9309..9314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ONECUT2"
                     /gene_synonym="OC-2; OC2"
                     /note="hexamer: AACAAA"
     polyA_site      9332
                     /gene="ONECUT2"
                     /gene_synonym="OC-2; OC2"
     regulatory      12210..12215
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ONECUT2"
                     /gene_synonym="OC-2; OC2"
                     /note="hexamer: ATTAAA"
     polyA_site      12228
                     /gene="ONECUT2"
                     /gene_synonym="OC-2; OC2"
     regulatory      16411..16416
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ONECUT2"
                     /gene_synonym="OC-2; OC2"
                     /note="hexamer: AATAAA"
     polyA_site      16433
                     /gene="ONECUT2"
                     /gene_synonym="OC-2; OC2"
                     /note="major polyA site"
ORIGIN      
        1 agagcccttc tggacagctc ccgctcaccc aaacagaaga cgtcggcgcc ggagcgggct
       61 cggacatggc gaggctgcga gccggcccga gcggcggggc ccggtgatcc ctccctccct
      121 ccccgtcccc tcccctctcc cgcacgcacg ccccgtccgc ccccaccccg cccccacccc
      181 gggcgagccc gcccgcagcc cggggcgcac acccgcacgc gcactcctct ccactcactc
      241 ccgcgcccgc ccccactccc gcagccgagc cccgccacgc gcgccttgcc cgcccgccgg
      301 ccgcccccgc cgcccccgcc gcccccgggc cctgatggac tgaatgaagg ctgcctacac
      361 cgcctatcga tgcctcacca aagacctaga aggctgcgcc atgaacccgg agctgacaat
      421 ggaaagtctg ggcactttgc acgggccggc cggcggcggc agtggcgggg gcggcggcgg
      481 gggcggcggg ggcggcggcg ggggcccggg ccatgagcag gagctgctgg ccagccccag
      541 cccccaccac gcgggccgcg gcgccgctgg ctcgctgcgg ggccctccgc cgcctccaac
      601 cgcgcaccag gagctgggca cggcggcagc ggcggcagcg gcggcgtcgc gctcggccat
      661 ggtcaccagc atggcctcga tcctggacgg cggcgactac cggcccgagc tctccatccc
      721 gctgcaccac gccatgagca tgtcctgcga ctcgtctccg cctggcatgg gcatgagcaa
      781 cacctacacc acgctgacac cgctccagcc gctgccaccc atctccaccg tgtctgacaa
      841 gttccaccac cctcacccgc accaccatcc gcaccaccac caccaccacc accaccagcg
      901 cctgtccggc aacgtcagcg gcagcttcac cctcatgcgc gacgagcgcg ggctcccggc
      961 catgaacaac ctctacagtc cctacaagga gatgcccggc atgagccaga gcctgtcccc
     1021 gctggccgcc acgccgctgg gcaacgggct aggcggcctc cacaacgcgc agcagagtct
     1081 gcccaactac ggtccgccgg gccacgacaa aatgctcagc cccaacttcg acgcgcacca
     1141 cactgccatg ctgacccgcg gtgagcaaca cctgtcccgc ggcctgggca ccccacctgc
     1201 ggccatgatg tcgcacctga acggcctgca ccacccgggc cacactcagt ctcacgggcc
     1261 ggtgctggca cccagtcgcg agcggccacc ctcgtcctca tcgggctcgc aggtggccac
     1321 gtcgggccag ctggaagaaa tcaacaccaa agaggtggcc cagcgcatca cagcggagct
     1381 gaagcgctac agtatccccc aggcgatctt tgcgcagagg gtgctgtgcc ggtctcaggg
     1441 gactctctcc gacctgctcc ggaatccaaa accgtggagt aaactcaaat ctggcaggga
     1501 gaccttccgc aggatgtgga agtggcttca ggagcccgag ttccagcgca tgtccgcctt
     1561 acgcctggca gcgtgcaaac gcaaagagca agaaccaaac aaagacagga acaattccca
     1621 gaagaagtcc cgcctggtgt tcactgacct ccaacgccga acactcttcg ccatcttcaa
     1681 ggagaacaaa cgcccgtcaa aggagatgca gatcaccatt tcccagcagc tgggcctgga
     1741 gctcacaacc gtcagcaact tcttcatgaa cgcccggcgc cgcagcctgg agaagtggca
     1801 agacgatctg agcacagggg gctcctcgtc cacctccagc acgtgtacca aagcatgatg
     1861 gaaggactct cacttgggca caagtcacct ccaaatgagg acaacagata ccaaaagaaa
     1921 acaaaggaaa aagacaccgg attcctagct ggggcccttc actggtgatt tgaaagcaca
     1981 attctcttgc aaagaaactt atattctagc tgtaatcata ggccaggtgt tcttcttttg
     2041 tttttaatgg ctatggagtc caagtgcaag ctgaaaaatt aatctcttag aaccagacac
     2101 tgttctctga gcatgctaag catcccagaa acccaaatgg ggccttcctg gagcgagtta
     2161 attccagtat ggtgtcaacc aagctcggga ttgcttaaaa tatcatccat cccacttcag
     2221 gtcctgtcag cttcttgcag tcagagttcc tatgagtaac aataggagtt tggcctatgt
     2281 aaggactctg agtttaggct tccaagatac aacaataaga gaagaatcta gcaacgagaa
     2341 tgacctcatt tgctttccac atgcttagcc tcattatacc atgttatgtc caagttcaca
     2401 gccacaacat cagaatggta attactgagc acaagtttta aatatggacg ttaaaaaaaa
     2461 aaatccaagg acctgttttt ccaacccaga catcttttca ttgaatgatt tagaaagctt
     2521 taagttgatc cagcttacaa tttttttttt ctttacctcc tggaaatctc atatggtctt
     2581 ggatccgtca aaaaaaccag tcagttcact tgcgctcaaa gtatcaagca caacaaagat
     2641 aaacagaagt gaggaaggtt ctgggttcac tacatctgga ttttcaagac acctattgtg
     2701 aagtcattag ggaattgatg agaatatggc ttcaagcaca ttttgcagtt tgctacaaat
     2761 tctgttgtac ataatgcaga cgcacactca ggaggccaat ttaactgtta acagtgcatg
     2821 gagcgaatgc agcattttaa aagatctagg tttttttagg tcattaatgt gtccttggtt
     2881 gatcagtcat ctggtccctc ctactgtgtg ttatgaccac cacgtaatcc attctcgctc
     2941 tttctgattt ggggtttttc ctcatccatc ccattagtag ggatgttttc tgtgttttct
     3001 agcaagaaaa aaaaatcaat caatcaaacc tgcatacatg ttactcatga ctgtcatcta
     3061 gtcctaaatc tcttctgttg ttgaatcatc cttgcaaaac agctgaatac atctggagaa
     3121 aacacagcac accaaagaag cagaatactg caaaccaaag acatttatga cttgtcattt
     3181 tctagcctaa aaatactgtg attactttta gaaatcagaa aacctctgca actccgaatg
     3241 gcattcagct cttgcatttg gcgcatcatc gggctgagcg gaccagctac accaaggaca
     3301 ttagccaagc cacccagagg ggtggctttg ccacaccagt tgtcaccttc ccatagcaag
     3361 tggaagagcg cccacagaac tctgggagat tgcaaaggtc acaatgtgca tatttaccag
     3421 tgaatggccc cgggtggggc cacgtggggg tgttcaaagc aagccaaacg ctgcaatcat
     3481 tctttacaga cacttgagac tgactttttt atgaattact tagtcgaaac caaagaaact
     3541 ttttctgcac ctacttctgc aacaaacaaa actgtcccat taaaatgaat aaataaatcc
     3601 gtaaatcaat ggaaatcacc accaataaga aggaagcacg ccagaaaata aacgaaaaca
     3661 aaaacaggga gacacactgt gttcaaacag acctcttggg acattttttg gaagcagatt
     3721 ttaaagaaag ggttgagaca aagatagaaa taaggaagag cctcagtggc tgctgcttca
     3781 tttgacaact cacacggtaa tcttaaagct gaagattgtc tttaatttgt gcctatgcag
     3841 tttttcaaaa gaacacggaa cagagcaaca gaaacctcaa cagctacaat accaaagatg
     3901 aggatttctc acaccttttg tttcagttca ttatctcctc ttgcctggct aaaatactaa
     3961 tagcgccatt gaactgtata aaggtaatca attatgtttc tctgagcaac aaaaggaaag
     4021 ggccatttat ttgattttat tgtttcattt caattttgtc ttatggtttt ttgccccaac
     4081 atggaatctc tcaaaagttt ccatggactc caagtttaag atgttgggat attgaacagt
     4141 tctctctgct cagcagaggg tagggaataa cattatcact tgaatgttct ttgcttaacc
     4201 cttagacttg gttccttcta tgttcagagt ctcatcatca ggggaaggaa agggagtgag
     4261 ggtcagggat aggggtcttg gtgatgcatc ctctcccgag ccacagaacc aaagagttta
     4321 tagaggaatt tacagcctcg ttttcatgtg attgctacat cctaacaggg cttcatttgg
     4381 gggtgggggg aaacatgtaa aaataattgc cagtttctac ttttctatta gctttttaaa
     4441 aatcagctgt aaagttgcat ttctaaagaa agatatatat aatatataaa atacatatat
     4501 agatcaactt gacattggtg ataaccaaaa ttattgctgt ccaaattcat gtcttgtttt
     4561 ggtccagtgc ttcatttgct aagtattcgg ttcagaattt ttctcatttc tcatgccatt
     4621 ccagagttaa tttgccactg tggatgattt gaagtattca gatctctatg gaagtttctg
     4681 ggacaggttt aaagtcaaga tcaagcattt tagcatttaa cctgttgata aatggatcca
     4741 tggtgtacat gagttttatt tgtattcgga gtcatctcta ttctatccct cagcctcgat
     4801 taaggtggtg agtgaagtgc atccaacaga ctcggcccag aactgggtcc tgacagtggg
     4861 gtgctcatct tctgtaactg ttgggaaggc tcggtggtcc attttcacca gttaaagaat
     4921 atgaggccag cccagaaatc tgttctccag gagctgccct gtcccatctg ggtgtgccag
     4981 accccctcag tgagcaggtc caccaaaggg acttctcaca ggggaagccc aactcctgtt
     5041 gcaatgggtt gatagatttc ctcagggtgg taattaccaa ttcgtatttt gacaagccta
     5101 tgtgcaacca cagctggcac tggggtgggc agtggtgttg ggtgggatgg gggagagtgt
     5161 ctcaatcctg aagagaaaat ataaagcagg ttttggggag acttctggag tcctgcccct
     5221 agagagcccc attgttgttc tttgtgcccc ctcctcattc cccctatgtg ggtctcccta
     5281 tgcaggagct gtgagagaat gtgactctcc acaattttta taattcatcc ttcctaggag
     5341 attgttcatt ggctcttccc ttgtgtccct ttgtcccttg ctcatactcc atgtttcctt
     5401 tgtcaaagga ctaagaaaag agcatatttc agcagaggag tgttcccatg tgggttgatt
     5461 tcaacttggg tatttctaaa agagtccttg tgacatgtgt ccagtggaaa tggttgctct
     5521 tttccagact ggattgagga atggagcctg tttgatttgg ttagtgattc tttgacatac
     5581 taatctcagc gtttgggtct ccagcatcct ctgaagatgt ctagactagt agaggctgcc
     5641 tttgtgacct gacattacaa cattggtcaa accagtcctc tgataatcag aagaacatgt
     5701 cataattgtt taaaaaaaaa aaaaaggcaa gaatttctct ccaaggagct ttaataaatg
     5761 tctcattcca gataatgtca taccagagaa aagtgcttgc ttttagaaaa ttatttacat
     5821 acatatataa atatatatgt gtatctatac agttatgtat caaaatttta agccctgcag
     5881 aatttcaatt tgttagaaat ctaacagaaa aaaatttcta tattgaaagg taatagaatt
     5941 taacccagtg agtttactca aggattttta aatttaagtt aataatttca gagaaaataa
     6001 ccatttgggt gtggttatag tttagtatcc attacctcaa tccaaggaaa attccaggca
     6061 ttcctcaacc atcaggaaaa ggtacagtgt gaaggaacag ttctcagcca aatttcacat
     6121 tcttgaggca acagaaatca aaacactcag agccattgag tggaaaaaca atttacttta
     6181 ttcctttaca caaataggct tgcattgttt ttgttttaat gtgattttgg tactagggat
     6241 ataattattt cattccagga aataataaaa aaaaacagac agagccaata catttctttt
     6301 tttaaaggaa acagcaacaa caataaaaac tcagcaccaa tatttaaaag cttttccaaa
     6361 atgtaaaaga agtgtttagc ttgcaccatg cataaaggtg caggctagtt gaaccaggaa
     6421 gcatggcact tcctctggag aaatccagaa agagttgctt ctaagctccc ttttccccct
     6481 gcaggctctt ggcaattgta ggctttagca aatccagaat aattttcaat tcaagctaaa
     6541 ataaaatcaa catttggaat gtaaatctga tacacacaca cttttctaag tcaaacaaca
     6601 tatttcaaaa ccaaaaataa atacctttta gataatcagt tattttcttt gtctatactg
     6661 ggcacccacc tactagtgcc agtaaattca agttgaacag atttttaaaa tcactattat
     6721 ctgggtatgg gggaaacttc cccacttttg aaaatgttgg tagaattata ggaatgtctg
     6781 tttgattatc attaccaaag tgtcatgaca gtatgccttt gtagtgaact cggattttca
     6841 ggagtttgaa tagttggata ttttaaaatc taagaagaaa aggcctgttt ccaatgttgt
     6901 tgaagaataa tgaactctat taaaaagtgg agaaaaagat aatacatgtg gtcaaggttg
     6961 accacaaggc ccaggcacaa ctaccttggc gataatcttc tagattcgta acaggttaga
     7021 gctgactttt tgtttttgtt gttgctgatg ctgtgtgatt cagacttctc agcctaacca
     7081 ggaagagtaa gtggaaatgg tagatgaaga aggggtagag ctggtgtatc tataactttc
     7141 tgatatttgt ctgccaaact tgatatatta gtaatttttt tatctttagc taagatcaag
     7201 tcacccctga aacaacagga gattctagtt ttaaaataag gccacaaaaa tccttacgga
     7261 atgaagaatg gcaccccagt tggttgtata agtctcataa gataatgatg ttgattttaa
     7321 atatggatgt ctcaatgcct gttttctatc aatgatttgt ttgtttccaa ggtcggggag
     7381 ggaaagaggg gagggtttat ctgttttaga aagtctcaga atacttataa aatacagaag
     7441 tagttattaa aatatatagg acctcacata ggtagataca gaacttacca ttgaggctga
     7501 tgggctgttg tgtgaatcac acaggacctt aaatgaggct cattattctc acacaccaaa
     7561 atgactctga cagcctgaag cagttattgc tagagcccaa gctttccttg gaggttttgg
     7621 agttaggttg attggaagta accagctaat accttttcta gtggagaaaa agacattgct
     7681 accagcttgt tcatcccata gaagtcttcc actctgctcc atttttagca gcaagcattt
     7741 catgtagcat aaaccttggc agataagtgt gcctaaggtt tatacagtct gtccgcttgg
     7801 atgtatacaa atttagatac atattttaac atgtgttctc atagatgact ttataacaac
     7861 acacattacc tataggtgtc tagactgtgt acatacaagt gtgtacagac aagcttcata
     7921 cgtatatact gtaatccgtt acaacaaata aattttaaat catcgtttaa catgtatgtg
     7981 gtacttctac agtgtacatt gttttcatta tttattgtaa cattgaaaac cacagtgcag
     8041 ggaaaacaaa agtatcccag catcttcatc ctgtacactt ggaattaatt tcatttgggc
     8101 atatccaaga taaactcaac tttcaagaaa tcttgtatat tatttaatca tctgtgttag
     8161 gatgacacct atgattgatg acttcggttg aatagcttta ttctggattt ttcataacta
     8221 aagctaaatc caaagacctg aaaaaggaca aaaagaaaaa aaaaaaaaga aaaaacaaag
     8281 aaaaagaaga aaaaataata aagtcaagcg caaactgatg gggagacagt gggctctggt
     8341 ttccaggatt gagacaatgg tactgcggtc ttggggagac tgcgttagct agtggggagt
     8401 ggtgattttt ttcatgcttg tcacatctaa atggtcttta acatgagaaa gttttagagg
     8461 ttataatttc ctgctttgtt tttatttaga ctatcaaatg aagttataca tgttgtcagt
     8521 caaaaaatga agacaccctc tgccccaccc cacagaatgc tttttatctt gtctctttgg
     8581 gttatgaccc aacaagctaa gtaccattaa tgtaattaac ttatttaaat tagttcctag
     8641 tacataaatg tataggattt gggtaattat ttaatcatcc ttccttagtt tgattctact
     8701 ccttgtactt atttatcaaa acctagacca atggtgcatc agagatgcaa aattctactt
     8761 ggaatactct tgaagtttag tttgctttat aaagcagtga aattctgtta cagacaggga
     8821 agaaatacag gttacaaaaa gagaatttgg gatattcttc cctcttaaat taacttttaa
     8881 aatagtctaa gtaacaattt ttaaattatt taacttaagt tcgcagcccc acctggtacc
     8941 aggcgaactt cacctcttaa ttattgtggc cctcggagcc ttcatattgt aacttattta
     9001 tttaacttat tcagcatctg tgaaaggtgc actgtatagt ttatattttt aatttaaaac
     9061 aacagagagc actgcagttt gtttgctgtc agaacaacag agcaaatttt gtggacaagc
     9121 aatgactatt cagcctgaac ctgtgcattc agaaaacata agctgagacc ctgcttcacc
     9181 agcctggatt tcggggcttc tatacagaaa ctggaaaaat aaattttaaa aaaatcgtaa
     9241 acaaaaagag agaaaccctt acactagctg cttccaagaa tgaactctgt gtgtatgtaa
     9301 agcaacaaaa caaaaaagga aaaaaacaaa aagcagaaaa aagaaaaaaa aaatgaaaaa
     9361 ctttctattt ctagtgagaa ccaaagaagg ctacctcact gactttttcc atttgtaatt
     9421 ttaatcgtgt tgatgacacc aaagatacca aagatttctt tctctgtgcg gtctgcattt
     9481 tgcttgtgct cttttataat ttgaacgatt ttctctgaca tatggtatgt acagccacag
     9541 ctcagatacc ccaaagaaat aattatctat gcgacggcgg ctgctaattt ggaaagggat
     9601 attttctgtg tttctcttat atgtttgctg tctgctcgac atgttcaaga tgcgagttca
     9661 gatgctgctg taattggatt ccttaaattc tgattacaaa ttgaggaagg aaactggttg
     9721 gaaatggcct tcagtcctag ccatggcctc tatccccgct gggacctgtc acagtaaaga
     9781 ctgccaatta ctgaaccaca gaagctctga ccattgagta gttgagctgg aagagacctt
     9841 aggaatcatt tagtccaagc cccggtggcc cagaggaatg aaatagttat ccaaatcaaa
     9901 taactcttga gagtgaaagc ccacacatgc ctcctggttc ctgccccagt gctccgctta
     9961 ttgtacagtg ctacctctgc atgagagcgg tcccacattg acaaatagga tggtggcaat
    10021 cctttagcaa tgagcaggga ctggggttta tctcttaaca ttttcagctg taaaattagt
    10081 cacaagcatt ttcagtgtcc cattagtaca tagtcacata tggtcggttg cttcgtgaag
    10141 gtggcctgtc ttgaaatact agggctcata cgggattttt gccctaggaa aaacatgttg
    10201 atcccaatga tgtgatcact tttgaacctt tccattacaa agcattgtat agataacttt
    10261 ttaattcagt aggaggagaa agttcattct tggcctgttg gctttgatta ttatgggtac
    10321 tttaaagtca gtatttatca agaaagggaa cttgaccacc attggcacat gtgacattta
    10381 agctcttcag ccttttcctt tttagttgta ggtgtttaca tttcatttct aagccaactc
    10441 tgtatttatg agagaagttt aagccttaca tcatttgata ctaaagggtt atttgtggta
    10501 aatgaaaaat gaccccaaaa ttacagagga atatgccagt ttaagaaatg gctacttaaa
    10561 gttgcttctc tctttccttc ttactcatga aattaattgg tcttcttcaa gtttctttag
    10621 attccattaa atgattaaat cactattaag agccattcat caacgtgatt tgtgtgttag
    10681 ccaatgaatc tgtctcagct tttgaccaaa tgggttttag acaaatgcaa agatctgcct
    10741 ctagtccata tggctctttt tgagtgctag tattttgcat ttcacataat gtagttattt
    10801 tgagctttta aagagagcat ttagacaaag aagcaaagag aggaagggac caatcaactc
    10861 atcagttcca tgcatcaaca aagcatagct agtagaggaa tataaatgac agattgacaa
    10921 actgtaggaa acactgttac tctctttctg aagttttcaa gcaccatcct atgtgaaagt
    10981 tccctcctgt ccaaacaagc tcaaggccca tcttctccct atacaaggca aacctgtaag
    11041 gccttccttc caaagagtac attgctttgg ttttcttcct aaattcctat tggaattaga
    11101 actctcagaa tccctgggag acagagcaaa gatgacttaa ttcattgagc agcagagctc
    11161 cctataagtg aacatcacct tccccatctt tcctactgcc acacccatac gagagaggat
    11221 ctagaaagag cgatggcagc ctgaacacag aaaacatccc cacttggcag acctctcctc
    11281 agcaatcccc ccagcctcat gcttcacttg caaagtgtga cataaccacg ggacgagtgc
    11341 cttgcttgaa ccaaagcaac gatttagcca gtctggacct ctctgtgctt tttttaattc
    11401 ttcctgtgaa tacctcagct tcaactgggc ctccatacag tcagttggtg ggcttattgt
    11461 actgtggtgc tttgcaatgc aaccctgcaa agaacaagat ttgtactaat accaaaggtt
    11521 ctttctctat gtctcctcct ctgcctccct cgttcttccc ttttttctag ttcttcacgg
    11581 ttccaaagct ttactatgaa cctgggcatg ttggcaatgc agaccgcgca attccttacc
    11641 gaattttctc agatatacct catagacaat agtgtttaga gtaatgttat tatagcgtat
    11701 gtaataaatt attcactgtt tcttttggta actgtgattt aaaaaaagaa aaaagaaaaa
    11761 aaagctttat acgttttagg ttgtgctttt gtaatagatg aaaaaaggtg cgcttaaaaa
    11821 gaaaatgtat gtttttttcc ccctttggat tttatttatg ctggattggg gaaagttgca
    11881 gaatgagccc aaagtttaca gtttcatatt ttgctgaaga aacaatctgt gttcatttgc
    11941 tctgttgaaa agaataatta ttttctacat ttgtgccact tggtctgaac aattaattgt
    12001 tccgtgttaa cagtgtagta ttatgattag caactgccaa tcagtgctat aattttatgc
    12061 atgaggctaa aaatttagca gtgtgatgca ttgtggtctt aatagcaaca tttttcattt
    12121 tgaactagat cttccccttt ggttcaatgg actttattta tgcatgggcg cctattgttt
    12181 gttagcagtt gtggaacagt tgtgtataca ttaaactgtg aaaatgtaca cagttcagcc
    12241 tcagacggtg gtaatattgg ttttattggg agatgtgtca cctcgaaaat accctttaca
    12301 tctgttggga tctgaaaatg agtcacattg aattgggttc cagctttata atgagaaacg
    12361 ttattcctaa tttttgagtt agccaatttg cattccacaa attgggatcc tcataaccca
    12421 aatatatcac cgtatgtgag agggatttga aagcgagtat tgaaaaactc acctttgcat
    12481 atttaatttc caccaaaagg agttattttg gctttatgct catgaactta gacctaactg
    12541 gccatgtata tgtagatgca aattcatcta gctgtggccc tctttgatct ctgcttggga
    12601 atggctattt ttgactatgc gtggtttctt ctcgtatttt gtgatcaggt cagctcccag
    12661 tagaaactca aatggcatca atattactaa ctcttctctg cccacttctc ttttgtccac
    12721 tctcctagac attcccacca actgttccag tgatttgggc aaaaatacgc agccatttcc
    12781 caaaacttca catgtgcagc tatcatggct gtccctccct agacttggag gtgactctca
    12841 cttaattttt acctgcccaa caatgttcca tctaccatct aaaaggtaat ataagaagaa
    12901 gttttgaaac ccactttagg aaaaccatct tctttaaatc cttcaattat ctgaggcctc
    12961 tatatgtcaa aactattttt cagttgcagg ggattgggca aacttgttct ttcttatact
    13021 tgggttcaaa gacccattct ccagtttcat atttcccaaa ccaaaatgct tgacataaag
    13081 ccaaatcaac tgccaagcac actttatttt gcataggagt atgcagccta gggaaccttg
    13141 gttgaaaagc agcagtctgc tatgcaaaat attggaaatc actgacagtg tagcattcat
    13201 attatctgtc aatgagggta tattgggaac gtgctctcgt gaataataaa aagcaacata
    13261 tttttatttg gccttataaa ttaggttgtg gtaatgtaaa ctttgatata tagtcttttt
    13321 atttttctct tattaatctg ccaaagatgg gaacagatac aagaattttt caaattggct
    13381 tttgtaagac aattgatgat tgtaatagtg tttaatcttc cagaaagctt tatatgttgt
    13441 tccacaataa aattgatatt tgtttcagca aagttttcct gacactcaca aacccacaaa
    13501 ctgttcctct taatgcagat attgtagaat ctacaaagtt caaatccatt tttgatccaa
    13561 agaaagtaga ggagtatttg agacatgagt gtacccagcc ctttttttaa tcacaggcaa
    13621 tgcatgggtc tggctggtta cactttgcca agaagacttg tcttatgaaa cccaaggtat
    13681 attttgttat gccattttat gtccttttct tttaacattg tggaaagtgg tatgttgaat
    13741 caagtgtaag ctgagttttc cagacaactg aagtagctac atcatgaatg ttattttgtt
    13801 attaaagggt ttttactcag tgctttgtgc caatggatgt ccttttcctt ggagacacat
    13861 aactacaaaa ttacctcagc ttggcctggt tttctctcct gccctcttgg ggaaacatgg
    13921 gcctggcctg ggaaaaggca ggtcatgggc tggaaggtag gttttggtac taggaagaaa
    13981 tctctgtatc tgtcagcttt aaagagaact gggccaaaaa tctctaacct cactctctct
    14041 ggactccaac acttccctgc aatcctttgg tcttgagcat gtgccagcat gaaggcagac
    14101 tccagttcat acatgaaagg caagaaaaag aaaatagtaa ccttgaatct tctgtgggcc
    14161 accaggcact cacctttccc caccttgcac actatccagt caaggctatt gcagcccatc
    14221 tggtggcttt acatgggaca ttaccaaagg cttcttcctc catcctgggg ttgcaaagga
    14281 tccaggtccc ctccatccag tggggctctt ccacatcaga agtccccctc ccaccatcct
    14341 ctgcatcctg tttagctatc ccatctatac cttttggaga tgattattta gaaaacaaag
    14401 aaaggtatgg aatggggttt cctattgttt gctaggttat attttagcaa ttctcaattc
    14461 tttgatctgg aaaaatacaa gagggaaaag gagaccccac tatctccctg tgctttgctc
    14521 ccatctcagg gggcaggggc agtgcacatt gcctatgctg ttgatctgtc ttgggcgaca
    14581 ggctgaatca cagctattgc cccagccaaa aacatggccc atcaatgcct actttatctc
    14641 tgcttgaaaa tcctattcaa aaagttgtag agtttgaggt ttttatcccc ccatatcctt
    14701 tgctttggtc cagtttggcc tttagcataa gagtcagctt tatctctagg aaagtttttt
    14761 cagattatga caaggaacct gccacctggg aagaaaagag tccgaagact agcaatcgga
    14821 taggtagtca taccattaac agatacttcc ttgaaggtag aatattattt cctttcttta
    14881 cagttttgtg ttacacaagt ccaagtggtg ccagcaaact tcttaccgtg aaatgttgta
    14941 aaacacctgg catactgaaa tttctgaaac aaaaacacaa gctccacatt gataacttga
    15001 taaataacca ctaaagttta gatgcaggga ctgagatgat acaggcaaaa tcttggtgtt
    15061 ggtttctctt ttaattcgta tcttcgatca cctaaccttt ctcaatccaa gagcagttca
    15121 gtcttttctc cccaagtcta ggatgccaaa gagcatcata ggaaaagata attagggatt
    15181 gaccagcatt tcaattagtt ctcttcttca tctttgcatt tctcaaaagt gttctcctgg
    15241 accagaggga aagagctggt ccattttttt tcattctttc tattcaaatt tttccaccca
    15301 gacaatactt tattaacaca gatactgtag atccttcctt ggtcagtgaa ttattacaag
    15361 aggagctatc cttccaccaa agtgagtgaa aacaagttcc agtatctttt cttccatcca
    15421 gttttgttct cagaatccaa gtcagtcctg ggtcttttct cactttagac cctggcctca
    15481 gatgtgttta ttcttgctat ttaaaaatac ctttaaattt cacatgctgg cctgcagaac
    15541 ttgcatcctt tgttctatac tgttgactgc ttgatggtat tgaaaggtga ctataatgag
    15601 ggaagaaagg aggaggtaaa gagagaagaa tttgtcccag atctgtttaa agtttcaaaa
    15661 tttaaaaagg gacccattaa attatgggaa aatggctata gagtgtgagc ctccgttgac
    15721 catatgctca aagaccgtac tctgccacct gccttccagg tagctattct agaaactcag
    15781 tcctttgtgg aaacccaact accttttaaa agtctctttc cagattccaa aaggacaaga
    15841 gatcagagag tcacatatac gcctcttgtt ttattttctt gctttcacgg gtattattgc
    15901 caagaaaatc gtagggaaaa actttaaact tttcttttca gttgatccct ttgacatcac
    15961 ctctcatgtt taaaatcagg aaaacacacc cctaaaattt gcactctctt ccgttttgaa
    16021 aaagaaaacc cacacacaaa tgcacactat taccgtcttt caccctgcgc tatatttcca
    16081 aagtgtatta taatccagat attgccccat ctcaaacatg ttaagtcaga ctgtgctgaa
    16141 agactttcca gggacggtca acagggtata tgttcagtgg ctgccctgaa atcctggtgg
    16201 ggatgaggat cacgcttcat catcaagggg atgcccatcc cctgataagc tcccagtcct
    16261 tttggaagat ttctttgaat gttaattgca ttttcagttt tgctcatttc ccaccccaat
    16321 gttttgtctg caacatcgct tacactggat tctttctatt tttattccta tcattaaatg
    16381 gtagtgctgt aaattctgca attaatgtta aataaactgc tttaattcat tga
//
