LOCUS       NM_002541               4193 bp    mRNA    linear   PRI 20-OCT-2024
DEFINITION  Homo sapiens oxoglutarate dehydrogenase (OGDH), transcript variant
            1, mRNA.
ACCESSION   NM_002541
VERSION     NM_002541.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Nguyen,T.T., Torrini,C., Shang,E., Shu,C., Mun,J.Y., Gao,Q.,
            Humala,N., Akman,H.O., Zhang,G., Westhoff,M.A., Karpel-Massler,G.,
            Bruce,J.N., Canoll,P. and Siegelin,M.D.
  TITLE     OGDH and Bcl-xL loss causes synthetic lethality in glioblastoma
  JOURNAL   JCI Insight 9 (8), e172565 (2024)
   PUBMED   38483541
  REMARK    GeneRIF: OGDH and Bcl-xL loss causes synthetic lethality in
            glioblastoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Whittle,E.F., Chilian,M., Karimiani,E.G., Progri,H., Buhas,D.,
            Kose,M., Ganetzky,R.D., Toosi,M.B., Torbati,P.N., Badv,R.S.,
            Shelihan,I., Yang,H., Elloumi,H.Z., Lee,S., Jamshidi,Y.,
            Pittman,A.M., Houlden,H., Ignatius,E., Rahman,S., Maroofian,R.,
            Yoon,W.H. and Carroll,C.J.
  TITLE     Biallelic variants in OGDH encoding oxoglutarate dehydrogenase lead
            to a neurodevelopmental disorder characterized by global
            developmental delay, movement disorder, and metabolic abnormalities
  JOURNAL   Genet Med 25 (2), 100332 (2023)
   PUBMED   36520152
  REMARK    GeneRIF: Biallelic variants in OGDH encoding oxoglutarate
            dehydrogenase lead to a neurodevelopmental disorder characterized
            by global developmental delay, movement disorder, and metabolic
            abnormalities.
REFERENCE   3  (bases 1 to 4193)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 4193)
  AUTHORS   Yap,Z.Y., Strucinska,K., Matsuzaki,S., Lee,S., Si,Y., Humphries,K.,
            Tarnopolsky,M.A. and Yoon,W.H.
  TITLE     A biallelic pathogenic variant in the OGDH gene results in a
            neurological disorder with features of a mitochondrial disease
  JOURNAL   J Inherit Metab Dis 44 (2), 388-400 (2021)
   PUBMED   32383294
  REMARK    GeneRIF: A biallelic pathogenic variant in the OGDH gene results in
            a neurological disorder with features of a mitochondrial disease.
REFERENCE   5  (bases 1 to 4193)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 4193)
  AUTHORS   McCartney,R.G., Rice,J.E., Sanderson,S.J., Bunik,V., Lindsay,H. and
            Lindsay,J.G.
  TITLE     Subunit interactions in the mammalian alpha-ketoglutarate
            dehydrogenase complex. Evidence for direct association of the
            alpha-ketoglutarate dehydrogenase and dihydrolipoamide
            dehydrogenase components
  JOURNAL   J Biol Chem 273 (37), 24158-24164 (1998)
   PUBMED   9727038
REFERENCE   7  (bases 1 to 4193)
  AUTHORS   Koike,K.
  TITLE     The gene encoding human 2-oxoglutarate dehydrogenase: structural
            organization and mapping to chromosome 7p13-p14
  JOURNAL   Gene 159 (2), 261-266 (1995)
   PUBMED   7622061
REFERENCE   8  (bases 1 to 4193)
  AUTHORS   Szabo,P., Cai,X., Ali,G. and Blass,J.P.
  TITLE     Localization of the gene (OGDH) coding for the E1k component of the
            alpha-ketoglutarate dehydrogenase complex to chromosome 7p13-p11.2
  JOURNAL   Genomics 20 (2), 324-326 (1994)
   PUBMED   8020988
REFERENCE   9  (bases 1 to 4193)
  AUTHORS   Koike,K., Urata,Y. and Goto,S.
  TITLE     Cloning and nucleotide sequence of the cDNA encoding human
            2-oxoglutarate dehydrogenase (lipoamide)
  JOURNAL   Proc Natl Acad Sci U S A 89 (5), 1963-1967 (1992)
   PUBMED   1542694
REFERENCE   10 (bases 1 to 4193)
  AUTHORS   Reed,L.J. and Hackert,M.L.
  TITLE     Structure-function relationships in dihydrolipoamide
            acyltransferases
  JOURNAL   J Biol Chem 265 (16), 8971-8974 (1990)
   PUBMED   2188967
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK314179.1, AK304690.1,
            AC004859.3 and BU682361.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002541.3.
            
            Summary: This gene encodes one subunit of the 2-oxoglutarate
            dehydrogenase complex. This complex catalyzes the overall
            conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA
            and CO(2) during the Krebs cycle. The protein is located in the
            mitochondrial matrix and uses thiamine pyrophosphate as a cofactor.
            A congenital deficiency in 2-oxoglutarate dehydrogenase activity is
            believed to lead to hypotonia, metabolic acidosis, and
            hyperlactatemia. Alternative splicing results in multiple
            transcript variants encoding distinct isoforms.[provided by RefSeq,
            Sep 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC004964.1, SRR1803614.1922.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000222673.6/
                                                  ENSP00000222673.5
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1504              AK314179.1         28-1531
            1505-3227           AK304690.1         1193-2915
            3228-4154           AC004859.3         49529-50455         c
            4155-4193           BU682361.1         22-60               c
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p13"
     gene            1..4193
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /note="oxoglutarate dehydrogenase"
                     /db_xref="GeneID:4967"
                     /db_xref="HGNC:HGNC:8124"
                     /db_xref="MIM:613022"
     exon            1..27
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            28..276
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..3126
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /EC_number="1.2.4.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; oxoglutarate dehydrogenase
                     (succinyl-transferring); oxoglutarate
                     (alpha-ketoglutarate) dehydrogenase (lipoamide);
                     2-oxoglutarate dehydrogenase, mitochondrial; oxoglutarate
                     decarboxylase; OGDC-E1; 2-oxoglutarate dehydrogenase
                     complex component E1; testicular tissue protein Li 131;
                     alpha-KGDH-E1; thiamine diphosphate (ThDP)-dependent
                     2-oxoglutarate dehydrogenase"
                     /codon_start=1
                     /product="2-oxoglutarate dehydrogenase complex component
                     E1 isoform 1 precursor"
                     /protein_id="NP_002532.2"
                     /db_xref="CCDS:CCDS34627.1"
                     /db_xref="GeneID:4967"
                     /db_xref="HGNC:HGNC:8124"
                     /db_xref="MIM:613022"
                     /translation="MFHLRTCAAKLRPLTASQTVKTFSQNRPAAARTFQQIRCYSAPV
                     AAEPFLSGTSSNYVEEMYCAWLENPKSVHKSWDIFFRNTNAGAPPGTAYQSPLPLSRG
                     SLAAVAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSS
                     VPADIISSTDKLGFYGLDESDLDKVFHLPTTTFIGGQESALPLREIIRRLEMAYCQHI
                     GVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEK
                     RFGLEGCEVLIPALKTIIDKSSENGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFD
                     SKLEAADEGSGDVKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQ
                     FYCGDTEGKKVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTVHVVVNNQIGFTT
                     DPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVAAEWRSTFHKDVVVDLVCY
                     RRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEISKYDKIC
                     EEAFARSKDEKILHIKHWLDSPWPGFFTLDGQPRSMSCPSTGLTEDILTHIGNVASSV
                     PVENFTIHGGLSRILKTRGEMVKNRTVDWALAEYMAFGSLLKEGIHIRLSGQDVERGT
                     FSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNAL
                     VLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERF
                     LQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLII
                     FTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTR
                     ERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPR
                     LRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS"
     exon            277..468
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            469..571
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            572..687
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            688..842
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            843..989
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1080
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1260
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1389
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1569
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1722
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1825
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1954
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2105
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            2106..2233
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            2234..2412
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            2413..2484
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            2485..2613
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..2686
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            2687..2850
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            2851..3005
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     exon            3006..4193
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4172..4177
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /note="hexamer: CATAAA"
     polyA_site      4193
                     /gene="OGDH"
                     /gene_synonym="AKGDH; E1k; E1o; HsOGDH; KGD1; OGDC;
                     OGDH-E1; OGDH2; OGDHD"
                     /note="major polyA site"
ORIGIN      
        1 attcgggtgg agctgagccg gagacaggca gttgtgaaaa acttcaggac aaaaatgttt
       61 catttaagga cttgtgctgc taagttgagg ccattgacgg cttcccagac tgttaagaca
      121 ttttcacaaa acagaccagc agcagctagg acatttcaac agattcggtg ctattctgca
      181 cctgttgctg ctgagccctt tctcagtggg actagttcga actatgtgga ggagatgtac
      241 tgtgcttggc tggaaaaccc caaaagtgta cataagtcat gggacatttt ttttcgcaac
      301 acgaatgccg gagccccacc gggcactgcc taccagagtc cccttcccct gagccgaggc
      361 tccctggctg ctgtggccca tgcacagtcc ctggtagaag cacagcccaa cgtggacaag
      421 ctcgtggagg accacctggc agtgcagtcg ctcatcaggg catatcagat acgagggcac
      481 catgtagcac agctggaccc cctggggatt ttggatgctg atctggactc ctccgtgccc
      541 gctgacatta tctcatccac agacaaactt gggttctatg gcctggatga gtctgacctc
      601 gacaaggtct tccacttgcc caccaccact ttcatcgggg gacaggaatc agcacttcct
      661 ctgcgggaga tcatccgtcg gctggagatg gcctactgcc agcatattgg ggtggagttc
      721 atgttcatca atgacctgga gcagtgccag tggatccggc agaagtttga gacccctggg
      781 atcatgcagt tcacaaatga ggagaaacgg accctgctgg ccaggcttgt gcggtccacc
      841 aggtttgagg agttcctaca gcggaagtgg tcctctgaga agcgctttgg tctagaaggc
      901 tgcgaggtac tgatccctgc cctcaagacc atcattgaca agtctagtga gaatggcgtg
      961 gactacgtga tcatgggcat gccacacaga gggcggctga acgtgcttgc aaatgtcatc
     1021 aggaaggagc tggaacagat cttctgtcaa ttcgattcaa agctggaggc agctgatgag
     1081 ggctccggag atgtgaagta ccacctgggc atgtatcacc gcaggatcaa tcgtgtcacc
     1141 gacaggaaca ttaccttgtc cttggtggcc aacccttccc accttgaggc cgctgacccc
     1201 gtggtgatgg gcaagaccaa agccgaacag ttttactgtg gcgacactga agggaaaaag
     1261 gtcatgtcca tcctgttgca tggggatgct gcatttgctg gccagggcat tgtgtacgag
     1321 accttccacc tcagcgacct gccatcctac acaactcatg gcaccgtgca cgtggtcgtc
     1381 aacaaccaga tcggcttcac caccgaccct cggatggccc gctcctcccc ctaccccact
     1441 gacgtggccc gagtggtgaa tgcccccatt ttccacgtga actcagatga ccccgaggct
     1501 gtcatgtacg tgtgcaaagt ggcggccgag tggaggagca ccttccacaa ggacgtggtt
     1561 gtcgatttgg tgtgttaccg gcgcaacggc cacaacgaga tggatgagcc catgttcacg
     1621 cagccgctca tgtacaagca gatccgcaag cagaagcctg tgttacagaa gtacgctgag
     1681 ctgctggtgt cgcagggtgt ggtcaaccag cctgagtatg aggaggaaat ttccaagtat
     1741 gataagatct gtgaggaagc ttttgccaga tctaaagatg agaagatctt gcacattaag
     1801 cactggctgg actctccctg gcctggcttc ttcaccctgg acgggcagcc caggagcatg
     1861 tcctgcccct ccacgggtct gacggaggat attctgacac acatcgggaa tgtggctagt
     1921 tctgtgcctg tggaaaactt tactattcat ggagggctga gccggatctt gaagactcgt
     1981 ggggaaatgg tgaagaaccg gactgtggac tgggctctag cggagtacat ggcgtttggc
     2041 tcgctcctga aggagggcat ccacattcgg ctgagcggcc aggacgtgga gcggggcaca
     2101 ttcagccacc gccaccatgt gctccatgac cagaatgtgg acaagagaac ctgcatcccc
     2161 atgaaccatc tctggcccaa tcaggccccc tatactgtgt gcaacagctc actgtctgag
     2221 tacggcgtgc tgggctttga gctgggcttc gccatggcca gtcctaatgc cctggtcctc
     2281 tgggaagccc aatttggtga cttccacaac acggcccagt gtatcatcga ccagttcatc
     2341 tgcccgggac aagccaagtg ggtgcggcag aatggcatcg tgttgctgct gccccatggc
     2401 atggagggca tgggtccaga acattcctcc gcccgcccag agcggttctt gcagatgtgc
     2461 aacgatgacc cagatgtcct gccagacctt aaagaagcca acttcgacat caatcagcta
     2521 tatgactgca attgggttgt tgtcaactgc tccactcctg gcaacttctt ccacgtgcta
     2581 cgacgccaga tcctgctgcc attccggaag ccgttaatta tcttcacccc caaatccctg
     2641 ttgcgccacc ccgaggccag atccagcttt gatgagatgc ttccaggaac ccacttccag
     2701 cgggtgatcc cagaagatgg ccctgcagct cagaacccag aaaatgtcaa aaggcttctc
     2761 ttctgcaccg gcaaagtgta ttatgacctc acccgggagc gcaaagcacg cgacatggtg
     2821 gggcaggtgg ccatcacaag gattgagcag ctgtcgccat tcccctttga cctcctgctg
     2881 aaggaggtgc agaagtaccc caatgctgag ctggcctggt gccaggagga gcacaagaac
     2941 caaggctact atgactacgt gaagccaaga cttcggacca ccatcagccg cgccaagccc
     3001 gtctggtatg ccggccggga cccagcggct gctccagcca ccggcaacaa gaagacccac
     3061 ctgacggagc tgcagcgcct cctggacacg gccttcgacc tggacgtctt caagaacttc
     3121 tcgtagatgc tgcctagggt tgcttgggcc actgccctct ccacacccat gactgcccct
     3181 tgcttctcaa ctaaagaata gtgcctcagc gctgcccaca ccaccgccct cctcgctgtg
     3241 ccaccacccc tccctctgct ctcataggag ttaggctgtc gtccccctcc agtgcttggc
     3301 tgccccacag gccacacgct gcccaggctc tgctgacttc tgagcagttt tccaggaggc
     3361 cggggggagc aggaggagga aaggtagccc ccgagggatg tccttgggga ggggtcagct
     3421 ctggccacaa tcctccccac cagtctcacc cactaggata ggaactgggc cttgtgtgct
     3481 ggcttccgct gtcacccagc aaggcacagg ctcctgtatt tgagactagg atagcttcat
     3541 cttgagcctg agccttagaa tctgtagagg agcctggagt cggatctagc catggctggc
     3601 agaggtttct agggtgggcc ccagccgtgg cgtgaactga ggatgacccg gggcagctgg
     3661 caggagagag ccttggcctg acctggcaca gaaagggcag cttcagtctc tgcagtgtcc
     3721 attatctgct gttccttcga gggttccagg ctgtgtgtgg ggcccaagca tgccccaccc
     3781 acccctcctg ggcccaggca gcacctggag cccacagagt ctgtgtgtag ccaggaagcc
     3841 ccgctcaggt agccaccacc ggggcactgg ctgctctgtc ttggtcctgt taaccctcca
     3901 cctcctctct tggactccct ccccacccca accactcttt ctttctcctt taacccaatg
     3961 gagactttct gatgcatcgt tttctttgct gtgccaaagc aggtcagaag agggagagga
     4021 ggggctgggg gtgaggggcc aggccatggc caaggggcca gctgcccctc atttatcact
     4081 ctgaccttca cagggacaga tctgatttat ttattttggt taaaaaaaaa aaaaaggaac
     4141 agaaacaact ttgcattgca ttggcttgac ccataaacta agttatatcc gtg
//
