LOCUS       NM_012215               5174 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens O-GlcNAcase (OGA), transcript variant 1, mRNA.
ACCESSION   NM_012215
VERSION     NM_012215.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Liu,Q., Chen,C., Fan,Z., Song,H., Sha,Y., Yu,L., Wang,Y., Qin,W.
            and Yi,W.
  TITLE     O-GlcNAcase regulates pluripotency states of human embryonic stem
            cells
  JOURNAL   Stem Cell Reports 19 (7), 993-1009 (2024)
   PUBMED   38942028
  REMARK    GeneRIF: O-GlcNAcase regulates pluripotency states of human
            embryonic stem cells.
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Du,Y., Yang,Z., Shi,H., Chen,Z., Chen,R., Zhou,F., Peng,X., Hong,T.
            and Jiang,L.
  TITLE     E3 ubiquitin ligase UBR5 promotes gemcitabine resistance in
            pancreatic cancer by inducing O-GlcNAcylation-mediated EMT via
            destabilization of OGA
  JOURNAL   Cell Death Dis 15 (5), 340 (2024)
   PUBMED   38755129
  REMARK    GeneRIF: E3 ubiquitin ligase UBR5 promotes gemcitabine resistance
            in pancreatic cancer by inducing O-GlcNAcylation-mediated EMT via
            destabilization of OGA.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5174)
  AUTHORS   Lu,P., Liu,Y., He,M., Cao,T., Yang,M., Qi,S., Yu,H. and Gao,H.
  TITLE     Cryo-EM structure of human O-GlcNAcylation enzyme pair OGT-OGA
            complex
  JOURNAL   Nat Commun 14 (1), 6952 (2023)
   PUBMED   37907462
  REMARK    GeneRIF: Cryo-EM structure of human O-GlcNAcylation enzyme pair
            OGT-OGA complex.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5174)
  AUTHORS   Luanpitpong,S., Rodboon,N., Samart,P., Janan,M., Klaihmon,P.,
            Lorthongpanich,C., U-Pratya,Y. and Issaragrisil,S.
  TITLE     Inhibition of O-GlcNAcase Inhibits Hematopoietic and Leukemic Stem
            Cell Self-Renewal and Drives Dendritic Cell Differentiation via
            STAT3/5 Signaling
  JOURNAL   Stem Cells 40 (12), 1078-1093 (2022)
   PUBMED   36124999
  REMARK    GeneRIF: Inhibition of O-GlcNAcase Inhibits Hematopoietic and
            Leukemic Stem Cell Self-Renewal and Drives Dendritic Cell
            Differentiation via STAT3/5 Signaling.
REFERENCE   5  (bases 1 to 5174)
  AUTHORS   Cui,Y., Xie,R., Zhang,X., Liu,Y., Hu,Y., Li,Y., Liu,X., Yu,X. and
            Wu,C.
  TITLE     OGA is associated with deglycosylation of NONO and the KU complex
            during DNA damage repair
  JOURNAL   Cell Death Dis 12 (7), 622 (2021)
   PUBMED   34135314
  REMARK    GeneRIF: OGA is associated with deglycosylation of NONO and the KU
            complex during DNA damage repair.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5174)
  AUTHORS   Farook,V.S., Bogardus,C. and Prochazka,M.
  TITLE     Analysis of MGEA5 on 10q24.1-q24.3 encoding the beta-O-linked
            N-acetylglucosaminidase as a candidate gene for type 2 diabetes
            mellitus in Pima Indians
  JOURNAL   Mol Genet Metab 77 (1-2), 189-193 (2002)
   PUBMED   12359146
  REMARK    GeneRIF: Investigated this locus in Pima Indians who have the
            world's highest prevalence of NIDDM. Concluded that mutations in
            MGEA5 are unlikely to contribute to NIDDM in this population
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 5174)
  AUTHORS   Wells,L., Gao,Y., Mahoney,J.A., Vosseller,K., Chen,C., Rosen,A. and
            Hart,G.W.
  TITLE     Dynamic O-glycosylation of nuclear and cytosolic proteins: further
            characterization of the nucleocytoplasmic
            beta-N-acetylglucosaminidase, O-GlcNAcase
  JOURNAL   J Biol Chem 277 (3), 1755-1761 (2002)
   PUBMED   11788610
REFERENCE   8  (bases 1 to 5174)
  AUTHORS   Comtesse,N., Maldener,E. and Meese,E.
  TITLE     Identification of a nuclear variant of MGEA5, a cytoplasmic
            hyaluronidase and a beta-N-acetylglucosaminidase
  JOURNAL   Biochem Biophys Res Commun 283 (3), 634-640 (2001)
   PUBMED   11341771
REFERENCE   9  (bases 1 to 5174)
  AUTHORS   Gao,Y., Wells,L., Comer,F.I., Parker,G.J. and Hart,G.W.
  TITLE     Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning
            and characterization of a neutral, cytosolic
            beta-N-acetylglucosaminidase from human brain
  JOURNAL   J Biol Chem 276 (13), 9838-9845 (2001)
   PUBMED   11148210
  REMARK    GeneRIF: This protein is a cytosolic, neutral, O-GlcNAc specific
            hexosaminidase termed O-GlcNAcase.
REFERENCE   10 (bases 1 to 5174)
  AUTHORS   Heckel,D., Comtesse,N., Brass,N., Blin,N., Zang,K.D. and Meese,E.
  TITLE     Novel immunogenic antigen homologous to hyaluronidase in meningioma
  JOURNAL   Hum Mol Genet 7 (12), 1859-1872 (1998)
   PUBMED   9811929
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC010789.9.
            
            On Aug 31, 2019 this sequence version replaced NM_012215.4.
            
            Summary: The dynamic modification of cytoplasmic and nuclear
            proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and
            removal on serine and threonine residues is catalyzed by OGT (MIM
            300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes
            O-GlcNAc modifications (Gao et al., 2001 [PubMed
            11148210]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB014579.2, AF036144.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361464.8/ ENSP00000354850.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-621               AC010789.9         146521-147141       c
            622-673             AC010789.9         142252-142303       c
            674-771             AC010789.9         141727-141824       c
            772-902             AC010789.9         138881-139011       c
            903-1074            AC010789.9         136427-136598       c
            1075-1173           AC010789.9         134742-134840       c
            1174-1458           AC010789.9         132432-132716       c
            1459-1617           AC010789.9         128939-129097       c
            1618-2231           AC010789.9         127539-128152       c
            2232-2406           AC010789.9         126677-126851       c
            2407-2492           AC010789.9         122610-122695       c
            2493-2597           AC010789.9         121536-121640       c
            2598-2683           AC010789.9         120051-120136       c
            2684-2876           AC010789.9         119593-119785       c
            2877-3036           AC010789.9         116061-116220       c
            3037-5174           AC010789.9         113147-115284       c
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.32"
     gene            1..5174
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /note="O-GlcNAcase"
                     /db_xref="GeneID:10724"
                     /db_xref="HGNC:HGNC:7056"
                     /db_xref="MIM:604039"
     exon            1..621
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             423..3173
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /EC_number="3.2.1.169"
                     /note="isoform a is encoded by transcript variant 1;
                     hyaluronidase in meningioma; bifunctional protein NCOAT;
                     nuclear cytoplasmic O-GlcNAcase and acetyltransferase;
                     meningioma-expressed antigen 5; meningioma expressed
                     antigen 5 (hyaluronidase); beta-hexosaminidase;
                     beta-N-acetylhexosaminidase; beta-N-acetylglucosaminidase;
                     N-acetyl-beta-glucosaminidase;
                     N-acetyl-beta-D-glucosaminidase; protein O-GlcNAcase"
                     /codon_start=1
                     /product="protein O-GlcNAcase isoform a"
                     /protein_id="NP_036347.1"
                     /db_xref="CCDS:CCDS7520.1"
                     /db_xref="GeneID:10724"
                     /db_xref="HGNC:HGNC:7056"
                     /db_xref="MIM:604039"
                     /translation="MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAG
                     AGGAAVAGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYK
                     HRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQ
                     VSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTE
                     YCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPV
                     IWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKS
                     NMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFG
                     VPHQYSSRQVAHSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTT
                     LTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAE
                     SKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFY
                     LPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGM
                     FTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFR
                     GGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGV
                     GLPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWI
                     PFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSV
                     AKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGR
                     SL"
     exon            622..673
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            674..771
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            772..902
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1074
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1173
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1458
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1617
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..2231
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2406
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            2407..2492
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            2493..2597
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            2598..2683
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            2684..2876
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            2877..3036
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            3037..5174
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5157..5162
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /note="hexamer: AATAAA"
     polyA_site      5174
                     /gene="OGA"
                     /gene_synonym="MEA5; MGEA5; NCOAT"
                     /note="major polyA site"
ORIGIN      
        1 gcggataaac aggaagcggg cggtggaggc agcagcagag ggagagctcg gggcttggag
       61 gggaaacagc ggaagaccta agattatcgg gagggcagca gaggcagaga acgaggacag
      121 gacccttggc cgtcttcttc cagggaacga gaggtcacag cctcgctctc cgcttaggct
      181 tctggcgccc cagcttaaag ccgaggctgc ggctgacaaa gggctcgcgc cggtgccgcc
      241 gcccttctca tccgggcatt cgggtccctg cggagaggga gggggaaggg cagaggggga
      301 ggggaaggag ccggaggggc gcacacttgg agctgaagcc ctctccaggg ctccgggccg
      361 gtgccccaac ggacagaggt cgaggaggac ccgcagaggt ggcagcggcc gggggcagga
      421 ggatggtgca gaaggagagt caagcgacgt tggaggagcg ggagagcgag ctcagctcca
      481 accctgccgc ctctgcgggg gcatcgctgg agccgccggc agctccggca cccggagaag
      541 acaaccccgc cggggctggg ggagcggcgg tggccggggc tgcaggaggg gctcggcggt
      601 tcctctgcgg tgtggtggaa ggattttatg gaagaccttg ggttatggaa cagagaaaag
      661 aactctttag aaggctccag aaatgggaat taaatacata cttgtatgcc ccaaaagatg
      721 actacaaaca taggatgttt tggcgagaga tgtattcagt ggaggaagct gagcaactta
      781 tgactctcat ctctgctgca cgagaatatg agatagagtt catctatgcg atctcacctg
      841 gattggatat cactttttct aaccccaagg aagtatccac attgaaacgt aaattggacc
      901 aggtttctca gtttgggtgc agatcatttg ctttgctttt tgatgatata gaccataata
      961 tgtgtgcagc agacaaagag gtattcagtt cttttgctca tgcccaagtc tccatcacaa
     1021 atgaaatcta tcagtaccta ggagagccag aaactttcct cttctgtccc acagaatact
     1081 gtggcacttt ctgttatcca aatgtgtctc agtctccata tttaaggact gtgggtgaaa
     1141 agcttctacc tggaattgaa gtgctttgga caggtcccaa agttgtttct aaagaaattc
     1201 cagtagagtc catcgaagag gtttctaaga ttattaagag agctccagta atctgggata
     1261 acattcatgc taatgattat gatcagaaga gactgtttct gggcccgtac aaaggaagat
     1321 ccacagaact catcccacgg ttaaaaggag tcctcactaa tccaaattgt gaatttgaag
     1381 ccaactacgt tgctatccac acccttgcca cctggtacaa atcaaacatg aatggagtga
     1441 gaaaagatgt agtgatgact gacagtgaag atagtactgt gtccatccag ataaaattag
     1501 aaaatgaagg cagtgatgaa gatattgaaa ctgatgtact ctatagtcca cagatggctc
     1561 taaagctagc attaacagaa tggttgcaag agtttggtgt gcctcatcaa tacagcagta
     1621 ggcaagttgc acacagtgga gctaaagcaa gtgtagttga tgggactcct ttagttgcag
     1681 caccctcttt aaatgccaca accgtagtaa caacagttta tcaggagccc attatgagcc
     1741 agggagcagc cttgagtggt gagcctacta ctctgaccaa ggaagaagaa aagaaacagc
     1801 ctgatgaaga acccatggac atggtggtgg aaaaacaaga agaaacggac cacaagaatg
     1861 acaatcaaat actgagtgaa attgttgaag cgaaaatggc agaggaattg aaaccaatgg
     1921 acactgataa agagagcata gctgaatcaa aatccccaga gatgtccatg caagaagatt
     1981 gtattagtga cattgccccc atgcaaactg atgaacagac aaacaaggag cagtttgtgc
     2041 caggtccaaa tgaaaagcct ttgtacactg cggaaccagt gaccctggag gatttgcagt
     2101 tacttgctga tctattctac cttccttacg agcatggacc caaaggagca cagatgttac
     2161 gggaatttca atggcttcga gcaaatagta gtgttgtcag tgtcaattgc aaaggaaaag
     2221 actctgaaaa aattgaagaa tggcggtcac gagcagccaa gtttgaagag atgtgtggac
     2281 tagtgatggg aatgttcact cggctctcca attgtgccaa caggacaatt ctttatgaca
     2341 tgtactccta tgtttgggat atcaagagta taatgtctat ggtgaagtct tttgtacagt
     2401 ggttagggtg tcgtagtcat tcttcagcac aattcttaat tggagaccaa gaaccctggg
     2461 cctttagagg tggtctagca ggagagttcc agcgtttgct gccaattgat ggggcaaatg
     2521 atctcttttt tcagccacct ccactgactc ctacctccaa agtttatact atcagacctt
     2581 attttcctaa ggatgaggca tccgtgtaca agatttgcag agaaatgtat gacgatggag
     2641 tgggtttacc ctttcaaagt cagcctgatc ttattggaga caagttagta ggagggctgc
     2701 tttccctcag cctggattac tgctttgtcc tagaagatga agatggcata tgtggttatg
     2761 ccttgggcac tgtagatgtg acccccttta ttaaaaaatg taaaatttcc tggatcccct
     2821 tcatgcagga gaagtatacc aagccaaatg gtgacaagga actctctgag gctgagaaaa
     2881 taatgttgag tttccatgaa gaacaggaag tactgccaga aactttcctt gctaatttcc
     2941 cttctctgat aaagatggac attcacaaaa aagtaactga cccaagtgtg gccaaaagca
     3001 tgatggcttg cctcctgtct tcactgaagg ctaatggctc ccggggagct ttctgtgaag
     3061 tgagaccaga tgataaaaga attctggaat tttacagcaa gttaggatgt tttgaaattg
     3121 caaaaatgga aggatttcca aaggatgtgg ttatacttgg tcggagcctg tgacatttgt
     3181 tgacactgtg aactgtccaa aagtctctta actgcacctt gtgaatggta gttgaggtct
     3241 tcatacagtt cagcctctag aatggtaaca aatcagccaa ttggattcga aacaaagaag
     3301 actatgtaaa actcacccat cacactttga gactactcac tggttggaag aatatagtat
     3361 tgcagcaaat cctgtatgaa agagagatgt gggcttcctt tttgagtctt gtgttaggtg
     3421 ctgagacctt ttacatgggc ttatacaggg agagagtctt caataaatgt agtcagcact
     3481 attttctgca tccagtgtgg ttgcgtttct cacctgagag taatcaagat aacatctgtc
     3541 atcttccttg gtttattgag tgaaatgcct ctcagtctta ggggacatgg cagagatgaa
     3601 agaaagaaag agtgggtttc agaagtgtca gggtggagtg attccaagtg ggatggttgt
     3661 ggcattagtt taagctgaat aaataatttc aatttggggc agttattctg ctttttgtaa
     3721 agccgtggcc aattgtctcc tgtaatgact gttggttcag gcatgttgta ctttgtaggg
     3781 acaaatgtgc atttgtttgt ggcaaaagcc tacaattgac aaacttgtaa atttctttgt
     3841 atataaacta gctgtaacct gactatcctt tgtgtttact gtttttgtaa atttttttcc
     3901 tctataaatg aaagggtgtt ggttcagaat ggcactttga ataatgtaaa ccagtgaaaa
     3961 gtggattttc tttacttttg tctttgggtt tggggttgtt tttgttcttt ttgaagtttt
     4021 attattttta aagtgcctcc cacctaggcg taggccatga ccatttgggg tacgagagcc
     4081 taattttgta ggacttaatc tgttgaaaag tgcagttact tctggaaatt aacctcaata
     4141 ttaggtcagc atgtgaaatg ttggatttga catgtcaggt agggttcagg gactgattgg
     4201 tcccatttgc cctcaggtca gttgtttaat ctcaagacct gttactactg attttattaa
     4261 atcagagtct ttaattcttg catgtttgta tctaatttct gaatgaatga gcacacttta
     4321 accagttatt tacagttacc tttttccttt aaccggattg tgaaagcttc atgtatttta
     4381 atttagattc tgtgttttta agggttctga gcatgaagct ggcagatagt cggcaggact
     4441 cattttttca tcatggctgg ctgatttctc catagattga taacagtatt ttgttatctt
     4501 gcttctctgt agttttgcat cagctgttta actttgagct gagtgagggg agaggggtaa
     4561 agagaaagaa acttaagttt tctttcacag aactccacca ttgtgggctt tgagagagcc
     4621 ctaaagcatt gtacctagtg gtacctagtg acttccaacc aaagcctttg agtatgcact
     4681 aaataggtga gaagaaagga gagaaggttt ttaggttaga aacctttaac cgatagaagg
     4741 atatggtatg ttgtaaagct ggaaccaagt ttgcattttt gagggcttga gatgaaggga
     4801 agactcttac cagatagtaa gacagctgag ttttcctcag ttttctcgtc ttaacactag
     4861 tggacaattc tagcattttg tttggaggat ttcagagtta acctcatgga attcaggatt
     4921 ttttagcaag tttgcttttg gttttatctt ggcttttagt aatcatgttg gctggtctgg
     4981 tcacaggtga ctgtgaaaca gatgccctgg tcttgctttc atcactctag gatcatgaag
     5041 tgctatgcta tttcctggtt atgaatatta aggttggaat tacattttta ttgattgttt
     5101 ggatcagagc tcagttcctg tagaaaacga actgtaaaag accatgcaag aggcaaaata
     5161 aaacttgaag tgaa
//
