LOCUS       NM_015311               6105 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens obscurin like cytoskeletal adaptor 1 (OBSL1),
            transcript variant 1, mRNA.
ACCESSION   NM_015311 XM_001128426 XM_001131592 XM_051017 XM_938504
VERSION     NM_015311.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Tuysuz,B., Alp Unkar,Z., Turan,H., Gezdirici,A., Uludag Alkaya,D.,
            Kasap,B., Yesil,G., Vural,M. and Ercan,O.
  TITLE     Natural history of facial and skeletal features from neonatal
            period to adulthood in a 3M syndrome cohort with biallelic CUL7 or
            OBSL1 variants
  JOURNAL   Eur J Med Genet 64 (12), 104346 (2021)
   PUBMED   34597859
  REMARK    GeneRIF: Natural history of facial and skeletal features from
            neonatal period to adulthood in a 3M syndrome cohort with biallelic
            CUL7 or OBSL1 variants.
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Isik,E., Arican,D., Atik,T., Ooi,J.E., Darcan,S., Ozen,S., Simsek
            Kiper,P.O., Utine,E., Cogulu,O. and Ozkinay,F.
  TITLE     A rare cause of syndromic short stature: 3M syndrome in three
            families
  JOURNAL   Am J Med Genet A 185 (2), 461-468 (2021)
   PUBMED   33258289
  REMARK    GeneRIF: A rare cause of syndromic short stature: 3M syndrome in
            three families.
REFERENCE   3  (bases 1 to 6105)
  AUTHORS   Jacob,P. and Girisha,K.M.
  TITLE     Three M syndrome 2 in two Indian patients
  JOURNAL   Am J Med Genet A 185 (2), 614-616 (2021)
   PUBMED   33135300
  REMARK    GeneRIF: Three M syndrome 2 in two Indian patients.
REFERENCE   4  (bases 1 to 6105)
  AUTHORS   Simsek-Kiper,P.O., Taskiran,E., Kosukcu,C., Arslan,U.E.,
            Cormier-Daire,V., Gonc,N., Ozon,A., Alikasifoglu,A., Kandemir,N.,
            Utine,G.E., Alanay,Y., Alikasifoglu,M. and Boduroglu,K.
  TITLE     Further expanding the mutational spectrum and investigation of
            genotype-phenotype correlation in 3M syndrome
  JOURNAL   Am J Med Genet A 179 (7), 1157-1172 (2019)
   PUBMED   30980518
  REMARK    GeneRIF: The mutational spectrum of CUL7, OBSL1, and investigation
            of genotype-phenotype correlation in 3M syndrome has been reported.
REFERENCE   5  (bases 1 to 6105)
  AUTHORS   Pernigo,S., Fukuzawa,A., Beedle,A.E.M., Holt,M., Round,A.,
            Pandini,A., Garcia-Manyes,S., Gautel,M. and Steiner,R.A.
  TITLE     Binding of Myomesin to Obscurin-Like-1 at the Muscle M-Band
            Provides a Strategy for Isoform-Specific Mechanical Protection
  JOURNAL   Structure 25 (1), 107-120 (2017)
   PUBMED   27989621
  REMARK    GeneRIF: Crystal structure of the obscurin(-like-1):myomesin
            complex reveals a trans-complementation mechanism whereby an
            incomplete immunoglobulin-like domain assimilates an
            isoform-specific myomesin interdomain sequence.
REFERENCE   6  (bases 1 to 6105)
  AUTHORS   Hanson,D., Murray,P.G., Sud,A., Temtamy,S.A., Aglan,M.,
            Superti-Furga,A., Holder,S.E., Urquhart,J., Hilton,E., Manson,F.D.,
            Scambler,P., Black,G.C. and Clayton,P.E.
  TITLE     The primordial growth disorder 3-M syndrome connects ubiquitination
            to the cytoskeletal adaptor OBSL1
  JOURNAL   Am J Hum Genet 84 (6), 801-806 (2009)
   PUBMED   19481195
  REMARK    GeneRIF: Loss of OBSL1 leads to downregulation of CUL7 and results
            in primordial growth disorder 3-M syndrome.
REFERENCE   7  (bases 1 to 6105)
  AUTHORS   Fukuzawa,A., Lange,S., Holt,M., Vihola,A., Carmignac,V.,
            Ferreiro,A., Udd,B. and Gautel,M.
  TITLE     Interactions with titin and myomesin target obscurin and
            obscurin-like 1 to the M-band: implications for hereditary
            myopathies
  JOURNAL   J Cell Sci 121 (11), 1841-1851 (2008)
   PUBMED   18477606
  REMARK    GeneRIF: N-terminal Ig-domains of Obsl1 and Obscurin (Obsc) bind to
            titin-M10 and myomesin. Titin mutations, linked to limb-girdle
            muscular dystrophy 2J (LGMD2J) or Salih myopathy, weaken or
            abrogate titin-Obsc and titin-Obsl1 binding.
REFERENCE   8  (bases 1 to 6105)
  AUTHORS   Geisler,S.B., Robinson,D., Hauringa,M., Raeker,M.O., Borisov,A.B.,
            Westfall,M.V. and Russell,M.W.
  TITLE     Obscurin-like 1, OBSL1, is a novel cytoskeletal protein related to
            obscurin
  JOURNAL   Genomics 89 (4), 521-531 (2007)
   PUBMED   17289344
  REMARK    GeneRIF: report the cloning and characterization of OBSL1; OBSL1 is
            located on human chromosome 2q35 within 100 kb of SPEG, another
            gene related to obscurin
REFERENCE   9  (bases 1 to 6105)
  AUTHORS   Lanfranchi,G., Muraro,T., Caldara,F., Pacchioni,B., Pallavicini,A.,
            Pandolfo,D., Toppo,S., Trevisan,S., Scarso,S. and Valle,G.
  TITLE     Identification of 4370 expressed sequence tags from a
            3'-end-specific cDNA library of human skeletal muscle by DNA
            sequencing and filter hybridization
  JOURNAL   Genome Res 6 (1), 35-42 (1996)
   PUBMED   8681137
REFERENCE   10 (bases 1 to 6105)
  AUTHORS   Irving,M. and Holder-Espinasse,M.
  TITLE     Three M Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301654
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB475523.1, CK904385.1,
            EF063638.1 and F30329.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_015311.2.
            
            Summary: Cytoskeletal adaptor proteins function in linking the
            internal cytoskeleton of cells to the cell membrane. This gene
            encodes a cytoskeletal adaptor protein, which is a member of the
            Unc-89/obscurin family. The protein contains multiple N- and
            C-terminal immunoglobulin (Ig)-like domains and a central
            fibronectin type 3 domain. Mutations in this gene cause 3M syndrome
            type 2. Alternatively spliced transcript variants encoding
            different isoforms have been found in this gene. [provided by
            RefSeq, Mar 2010].
            
            Transcript Variant: This variant (1) is the longest transcript and
            encodes the longest isoform (1, also known as isoform A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EF063638.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000404537.6/ ENSP00000385636.1
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-228               DB475523.1         10-237
            229-250             CK904385.1         1-22
            251-6091            EF063638.1         1-5841
            6092-6105           F30329.1           347-360
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q35"
     gene            1..6105
                     /gene="OBSL1"
                     /note="obscurin like cytoskeletal adaptor 1"
                     /db_xref="GeneID:23363"
                     /db_xref="HGNC:HGNC:29092"
                     /db_xref="MIM:610991"
     exon            1..1319
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             308..5998
                     /gene="OBSL1"
                     /EC_number="2.7.11.18"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; obscurin-like protein 1; obscurin like 1"
                     /codon_start=1
                     /product="obscurin-like protein 1 isoform 1 precursor"
                     /protein_id="NP_056126.1"
                     /db_xref="CCDS:CCDS46520.1"
                     /db_xref="GeneID:23363"
                     /db_xref="HGNC:HGNC:29092"
                     /db_xref="MIM:610991"
                     /translation="MKASSGDQGSPPCFLRFPRPVRVVSGAEAELKCVVLGEPPPVVV
                     WEKGGQQLAASERLSFPADGAEHGLLLTAALPTDAGVYVCRARNAAGEAYAAAAVTVL
                     EPPASDPELQPAERPLPSPGSGEGAPVFLTGPRSQWVLRGAEVVLTCRAGGLPEPTLY
                     WEKDGMALDEVWDSSHFALQPGRAEDGPGASLALRILAARLPDSGVYVCHARNAHGHA
                     QAGALLQVHQPPESPPADPDEAPAPVVEPLKCAPKTFWVNEGKHAKFRCYVMGKPEPE
                     IEWHWEGRPLLPDRRRLMYRDRDGGFVLKVLYCQAKDRGLYVCAARNSAGQTLSAVQL
                     HVKEPRLRFTRPLQDVEGREHGIAVLECKVPNSRIPTAWFREDQRLLPCRKYEQIEEG
                     TVRRLIIHRLKADDDGIYLCEMRGRVRTVANVTVKGPILKRLPRKLDVLEGENAVLLV
                     ETLEAGVEGRWSRDGEELPVICQSSSGHMHALVLPGVTREDAGEVTFSLGNSRTTTLL
                     RVKCVKHSPPGPPILAEMFKGHKNTVLLTWKPPEPAPETPFIYRLERQEVGSEDWIQC
                     FSIEKAGAVEVPGDCVPSEGDYRFRICTVSGHGRSPHVVFHGSAHLVPTARLVAGLED
                     VQVYDGEDAVFSLDLSTIIQGTWFLNGEELKSNEPEGQVEPGALRYRIEQKGLQHRLI
                     LHAVKHQDSGALVGFSCPGVQDSAALTIQESPVHILSPQDRVSLTFTTSERVVLTCEL
                     SRVDFPATWYKDGQKVEESELLVVKMDGRKHRLILPEAKVQDSGEFECRTEGVSAFFG
                     VTVQDPPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRWYKDGQEVEESDFVVLE
                     NEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTITDVSSWIVYPSGKVYVAAVRLE
                     RVVLTCELCRPWAEVRWTKDGEEVVESPALLLQKEDTVRRLVLPAVQLEDSGEYLCEI
                     DDESASFTVTVTEPPVRIIYPRDEVTLIAVTLECVVLMCELSREDAPVRWYKDGLEVE
                     ESEALVLERDGPRCRLVLPAAQPEDGGEFVCDAGDDSAFFTVTVTAPPERIVHPAARS
                     LDLHFGAPGRVELRCEVAPAGSQVRWYKDGLEVEASDALQLGAEGPTRTLTLPHAQPE
                     DAGEYVCETRHEAITFNVILAEPPVQFLALETTPSPLCVAPGEPVVLSCELSRAGAPV
                     VWSHNGRPVQEGEGLELHAEGPRRVLCIQAAGPAHAGLYTCQSGAAPGAPSLSFTVQV
                     AEPPVRVVAPEAAQTRVRSTPGGDLELVVHLSGPGGPVRWYKDGERLASQGRVQLEQA
                     GARQVLRVQGARSGDAGEYLCDAPQDSRIFLVSVEEPLLVKLVSELTPLTVHEGDDAT
                     FRCEVSPPDADVTWLRNGAVVTPGPQVEMAQNGSSRILTLRGCQLGDAGTVTLRAGST
                     ATSARLHVRETELLFLRRLQDVRAEEGQDVCLEVETGRVGAAGAVRWVRGGQPLPHDS
                     RLSMAQDGHIHRLFIHGVILADQGTYGCESHHDRTLARLSVRPRQLRVLRPLEDVTIS
                     EGGSATFQLELSQEGVTGEWARGGVQLYPGPKCHIHSDGHRHRLVLNGLGLADSGCVS
                     FTADSLRCAARLIVREVPVTIVRGPHDLEVTEGDTATFECELSQALADVTWEKDGNAL
                     TPSPRLRLQALGTRRLLQLRRCGPSDAGTYSCAVGTARAGPVRLTVRERTVAVLSELR
                     SVSAREGDGATFECTVSEVETTGRWELGGRPLRPGARVRIRQEGKKHILVLSELRAED
                     AGEVRFQAGPAQSLALLEVEALPLQMCRHPPREKTVLVGRRAVLEVTVSRSGGHVCWL
                     REGAELCPGDKYEMRSHGPTHSLVIHDVRPEDQGTYCCQAGQDSTHTRLLVEGN"
     exon            1320..1589
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1841
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1842..2144
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..2441
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2714
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2715..2987
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2988..3260
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            3261..3533
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            3534..3809
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            3810..4097
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            4098..4373
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            4374..4643
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            4644..4916
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            4917..5183
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            5184..5296
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            5297..5453
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            5454..5615
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            5616..5720
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            5721..5990
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            5991..6105
                     /gene="OBSL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6086..6091
                     /regulatory_class="polyA_signal_sequence"
                     /gene="OBSL1"
                     /note="hexamer: AATAAA"
     polyA_site      6105
                     /gene="OBSL1"
                     /note="major polyA site"
ORIGIN      
        1 aagagcagga agccaggcag cgggcagggg aggctgcggg gccactcgct ggagaggcaa
       61 acaggaagga ctgccccctg agcgccaggc ttcgggcccg ggaatcgccg ccgccgccgc
      121 cgcagagctg cagctcgggg ccgagggtaa ggaggcgagc cgggagcggg aggcccggga
      181 gagctccgcg ggtccccgcg cccagtcccc agccgcgccc cgaccccgcc gccccgggcc
      241 tcggctcgcc ctccgcaccc ccccctgccc ccccaccgtt cgccgctgca ggcggtcggc
      301 cgccgcgatg aaggcgagct cgggggatca ggggagcccc ccgtgcttcc tgcgcttccc
      361 gcggcctgtg cgggtggtaa gtggcgccga ggccgagctc aagtgcgtgg tcctggggga
      421 gccgccgcct gtagtggtgt gggagaaggg cgggcagcag ctggcggcct cggaacgcct
      481 gagcttcccg gcggacggcg cggagcacgg cctgctgctg accgccgcac tgcccaccga
      541 cgcgggggtc tacgtgtgcc gcgcccgcaa cgcggccggc gaggcctacg cggcggccgc
      601 cgtcaccgtg ctggagccgc cggcctccga ccccgagctg cagcccgccg agcgcccgct
      661 gccatcgccg gggtccgggg agggcgcccc ggtcttcctc acggggcctc gatcccagtg
      721 ggtgctgcgg ggggcggagg tggtgctgac gtgccgggcg gggggcctcc ccgagcccac
      781 actgtactgg gagaaggacg ggatggccct ggacgaagtg tgggacagca gccacttcgc
      841 gctccagccg ggccgcgccg aggacggccc cggcgcgagc ctggcactgc gcatcctggc
      901 ggctcggctg ccggattccg gcgtctacgt gtgccacgcc cgcaacgcgc acggccacgc
      961 gcaggcgggg gcgctgctcc aggtgcacca gccccccgag agcccgcccg cggaccccga
     1021 cgaggccccc gcgccggtgg tggagccgct caagtgcgcg cctaagacct tctgggtgaa
     1081 cgagggcaag cacgccaagt tccgctgcta cgtgatgggc aagcccgagc ccgagatcga
     1141 atggcactgg gagggccgcc cgctgctccc ggaccgccgc cgcctcatgt accgcgaccg
     1201 cgacggcggc ttcgtgctca aggtgcttta ctgccaggcc aaggatcgtg ggctctacgt
     1261 ctgcgccgcg cgcaactcgg cgggccagac gctcagtgcc gtgcagctgc acgtgaaaga
     1321 gccccgcctc cggttcacac ggcccctgca ggacgtggag ggccgtgagc acgggattgc
     1381 cgtgctggaa tgtaaagtac ccaactcccg catccccacg gcctggttcc gtgaggacca
     1441 gcggctgctg ccctgccgca agtacgagca gatcgaagag ggcactgtcc ggcgcctcat
     1501 catccacagg ctgaaggcag acgatgatgg tatctacctg tgcgagatgc ggggccgggt
     1561 gcgcaccgtg gccaacgtca cagtcaaagg gcccatcctg aagcgcctgc cccggaagct
     1621 cgacgtcctg gaaggagaga atgctgtgct gctagtggaa actctagagg ccggggtcga
     1681 gggacgctgg agccgtgatg gggaggagct gccggtcatc tgccagagca gctcaggcca
     1741 catgcatgcc ctggtccttc caggggtcac ccgagaggat gctggcgagg tcacctttag
     1801 cctgggcaac tcccgtacca ctacgcttct cagagtaaaa tgtgtcaagc acagtccccc
     1861 aggacccccc atattggcag agatgttcaa gggccacaag aacacggtcc tgttgacctg
     1921 gaagcctccc gagccagctc ccgagacccc attcatctac cggctggagc ggcaggaagt
     1981 gggctctgaa gactggattc agtgcttcag catcgagaaa gccggagccg tggaggtgcc
     2041 gggcgactgt gtgccctccg agggtgacta ccgcttccgc atctgcacag tcagcggaca
     2101 tggccgtagt ccccacgtgg tgttccacgg ttctgctcac cttgtgccca cagctcgcct
     2161 ggtggcaggt ctggaggatg tgcaggtata cgacggggaa gatgccgtct tctccctcga
     2221 tctctccacc atcatccagg gtacctggtt ccttaatggg gaagagctca agagtaacga
     2281 gccggagggc caggtggaac ctggggccct gcggtaccgt atagagcaga agggtctgca
     2341 gcacagactc atcctgcatg ccgtcaagca ccaggacagc ggtgccctgg tcggcttcag
     2401 ctgccccggc gtgcaggact cagctgccct cacaatccaa gagagcccgg tgcacatcct
     2461 gagcccccag gacagggtgt cgttgacctt cacaacctca gagcgggtgg tgctgacttg
     2521 tgagctctca agggtggact tcccggcaac ctggtacaag gatgggcaga aggtggagga
     2581 gagcgagttg ctggtggtga agatggatgg gcgcaaacac cgtctgatcc tgcctgaggc
     2641 caaagtccag gacagtggcg agtttgagtg caggacagaa ggggtctcgg ccttcttcgg
     2701 cgtcactgtc caagatcctc ccgtgcacat cgtggacccc cgagaacatg tgttcgtgca
     2761 tgccataact tccgagtgtg tcatgctggc ctgtgaggtg gaccgagagg acgcccctgt
     2821 gcgttggtac aaggacgggc aggaggtgga ggagagtgac ttcgtggtgc tggagaatga
     2881 ggggccccat cgccgcctgg tgctgcccgc cacccagccc tcagacgggg gcgagtttca
     2941 gtgcgtcgct ggagatgagt gtgcctactt cactgtcacc atcacagacg tctcctcgtg
     3001 gatcgtgtat cccagcggca aggtgtatgt ggcagccgtg cgcctggagc gtgtggtgct
     3061 gacctgtgag ctatgccggc cctgggcaga ggtgcgctgg accaaggatg gagaggaggt
     3121 ggtggagagc cccgcgctgc tcctgcagaa ggaagacact gtccgccgcc tggtgctgcc
     3181 cgctgtccag ctcgaggact ccggcgagta cttgtgtgaa attgacgatg agtcggcctc
     3241 cttcactgtc accgtcacag aacccccagt gcggatcata taccctcgcg atgaggtgac
     3301 cttgatcgcc gtgaccttgg agtgtgtggt gctgatgtgt gaactgtctc gggaggatgc
     3361 ccctgtgcgc tggtacaagg atgggctgga agtggaggag agcgaggccc tggtgctgga
     3421 gagggatggg ccacgctgcc gcctggtgct acctgctgct cagcccgagg acgggggcga
     3481 gtttgtatgt gatgctggag atgactcggc cttcttcact gtcactgtca cagccccacc
     3541 agagaggatt gtgcacccgg cagcccgctc cctggatctg cattttgggg ctccagggcg
     3601 cgtggagctg cgctgtgagg tggccccagc tgggtctcag gtgcgctggt acaaggacgg
     3661 gctggaagtg gaggcatcag atgccctgca gctgggtgcc gaggggccca cccgcaccct
     3721 gaccctgccc cacgcccagc ctgaggacgc cggggagtat gtgtgtgaga cccggcatga
     3781 ggccatcacc ttcaatgtca tcctggctga gcctccagtg cagttccttg ctctagagac
     3841 aactccaagc ccgctctgtg tggcccctgg ggagccagtg gtgctgagct gtgaactgtc
     3901 ccgggctggc gcccccgtgg tctggagcca caatgggagg cccgtgcagg agggcgaggg
     3961 cctagagctc catgccgagg gcccccgccg agtcctctgc atccaggctg caggcccagc
     4021 ccatgcaggg ctctacacct gccagtctgg agcagccccc ggagccccaa gcctcagctt
     4081 caccgtccag gtggctgagc cccctgtgcg ggtggtagct cccgaggcag cccagacgag
     4141 ggttcggagc accccaggcg gggacctaga gctggtggtg cacctctccg ggccaggggg
     4201 ccctgtacgc tggtacaagg acggggagcg actggcaagc caggggcggg tgcagctgga
     4261 gcaggccggg gccaggcagg tgctgcgggt gcagggggca cggagcgggg acgctgggga
     4321 gtacctgtgc gatgcgcccc aggacagccg catcttcctt gtcagcgtgg aagagccact
     4381 gctggtgaag ctggtctcgg agctgacacc actcactgtc cacgagggcg atgatgccac
     4441 gttccggtgt gaagtctccc caccagatgc cgatgtcacc tggctgcgca atggggccgt
     4501 cgtcactcca gggccccagg tggagatggc ccagaatggt tcaagccgca tcttaacctt
     4561 gcgaggctgc caactggggg atgcagggac cgtgactttg cgggcaggga gcacggccac
     4621 aagtgcccgg ctccatgttc gagagacaga gctgctgttc ctacggcggt tgcaggatgt
     4681 gcgggcagag gaaggccagg atgtgtgtct cgaagtggag acaggccgag tgggtgcagc
     4741 gggggccgtg cgctgggtgc gaggtgggca gcccctgccc cacgactctc gcctgtccat
     4801 ggcccaggat gggcacatcc accgcctctt catccatggt gtcatactgg ccgaccaggg
     4861 cacctacggc tgcgagagcc accacgatcg caccctggcc aggctcagcg tgaggccgag
     4921 gcagctgagg gtgctgcggc ctctggagga cgtgaccatc agtgaggggg gcagtgccac
     4981 cttccagctg gagctgtccc aggaaggtgt gaccggggag tgggcccggg gtggagtaca
     5041 gctgtatcca ggacccaagt gtcacatcca ctcggacggc caccgtcacc gactggtact
     5101 caatggcctg ggcctggccg actcaggctg tgtctccttc acagcggatt ccctgcgctg
     5161 cgcagccaga ctcattgtga gagaggtccc agtgaccatc gtgcgggggc cacacgacct
     5221 agaggtgacc gagggcgaca cagctacgtt cgagtgcgag ctttcccaag ctttggctga
     5281 tgttacctgg gagaaggacg ggaacgcgct tacgcctagc ccgcggctcc ggctccaggc
     5341 cctcggcacg cgccgccttc tccagctgcg acgctgcggc ccctcggacg ccgggaccta
     5401 cagctgcgcg gtggggacgg cccgcgccgg accggtccgc ctgaccgtgc gcgagcgtac
     5461 tgtggcggta ctctccgagc tgcggtcggt gagcgcccgc gaaggcgacg gcgctacgtt
     5521 cgagtgcacc gtgtcggagg tcgagaccac ggggcgctgg gagctcggag gccgcccgct
     5581 gagacccgga gcccgcgtcc gcatccgaca ggaagggaag aaacacattc tggtgcttag
     5641 cgagctgcgc gccgaggacg ccggtgaagt ccgcttccag gcggggcccg cccagtccct
     5701 ggctctactg gaagtggagg cattgcctct ccagatgtgc cgccaccccc ctcgcgagaa
     5761 gaccgttctg gtgggccgcc gggcggtgct ggaggtgact gtgtcccgct cggggggcca
     5821 cgtgtgctgg ctgcgggagg gggccgagct gtgcccggga gataagtatg agatgcgcag
     5881 ccacggcccc acccacagcc tggtcatcca tgacgttcga cctgaggacc aaggcactta
     5941 ctgctgccag gccggccagg acagcaccca cacacggctg ctggtagagg gcaactagga
     6001 gaacctaacc aggccaggcg ggtgcccttg gacagcttgg aaggcgtttg cccttaccct
     6061 gggcaggggt agagagacaa ggaacaataa aagtgctaca gctca
//
