LOCUS       NM_016359               2263 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens nucleolar and spindle associated protein 1 (NUSAP1),
            transcript variant 1, mRNA.
ACCESSION   NM_016359
VERSION     NM_016359.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ge,Y., Wang,B., Xiao,J., Wu,H. and Shao,Q.
  TITLE     NUSAP1 promotes gastric cancer radioresistance by inhibiting
            ubiquitination of ANXA2 and is suppressed by miR-129-5p
  JOURNAL   J Cancer Res Clin Oncol 150 (8), 406 (2024)
   PUBMED   39212774
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Sun,M., Wang,Y., Xin,G., Yang,B., Jiang,Q. and Zhang,C.
  TITLE     NuSAP regulates microtubule flux and Kif2A localization to ensure
            accurate chromosome congression
  JOURNAL   J Cell Biol 223 (2) (2024)
   PUBMED   38117947
  REMARK    GeneRIF: NuSAP regulates microtubule flux and Kif2A localization to
            ensure accurate chromosome congression.
REFERENCE   3  (bases 1 to 2263)
  AUTHORS   Liu,Y., Tang,R., Meng,Q.C., Shi,S., Xu,J., Yu,X.J., Zhang,B. and
            Wang,W.
  TITLE     NUSAP1 promotes pancreatic ductal adenocarcinoma progression by
            drives the epithelial-mesenchymal transition and reduces AMPK
            phosphorylation
  JOURNAL   BMC Cancer 24 (1), 87 (2024)
   PUBMED   38229038
  REMARK    GeneRIF: NUSAP1 promotes pancreatic ductal adenocarcinoma
            progression by drives the epithelial-mesenchymal transition and
            reduces AMPK phosphorylation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2263)
  AUTHORS   Zhu,Y., Liu,Y., Zhang,L., Zeng,S. and Xu,W.
  TITLE     NUSAP1 regulates basal cell carcinoma migration, invasion and DNA
            damage through activation of the Hedgehog signaling pathway
  JOURNAL   Physiol Int 110 (2), 160-172 (2023)
   PUBMED   37200136
  REMARK    GeneRIF: NUSAP1 regulates basal cell carcinoma migration, invasion
            and DNA damage through activation of the Hedgehog signaling
            pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2263)
  AUTHORS   Chiu,C.L., Li,C.G., Verschueren,E., Wen,R.M., Zhang,D.,
            Gordon,C.A., Zhao,H., Giaccia,A.J. and Brooks,J.D.
  TITLE     NUSAP1 Binds ILF2 to Modulate R-Loop Accumulation and DNA Damage in
            Prostate Cancer
  JOURNAL   Int J Mol Sci 24 (7), 6258 (2023)
   PUBMED   37047232
  REMARK    GeneRIF: NUSAP1 Binds ILF2 to Modulate R-Loop Accumulation and DNA
            Damage in Prostate Cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2263)
  AUTHORS   Gulzar,Z.G., McKenney,J.K. and Brooks,J.D.
  TITLE     Increased expression of NuSAP in recurrent prostate cancer is
            mediated by E2F1
  JOURNAL   Oncogene 32 (1), 70-77 (2013)
   PUBMED   22349817
  REMARK    GeneRIF: NuSAP is a novel biomarker for prostate cancer recurrence
            after surgery and its overexpression appears to be driven in part
            by E2F1 activation
REFERENCE   7  (bases 1 to 2263)
  AUTHORS   Chou,H.Y., Wang,T.H., Lee,S.C., Hsu,P.H., Tsai,M.D., Chang,C.L. and
            Jeng,Y.M.
  TITLE     Phosphorylation of NuSAP by Cdk1 regulates its interaction with
            microtubules in mitosis
  JOURNAL   Cell Cycle 10 (23), 4083-4089 (2011)
   PUBMED   22101338
  REMARK    GeneRIF: Phosphorylation of NuSAP by Cdk1 is an important mechanism
            to regulate microtubule dynamics during cell cycle progression.
REFERENCE   8  (bases 1 to 2263)
  AUTHORS   Verbakel,W., Carmeliet,G. and Engelborghs,Y.
  TITLE     SAP-like domain in nucleolar spindle associated protein mediates
            mitotic chromosome loading as well as interphase chromatin
            interaction
  JOURNAL   Biochem Biophys Res Commun 411 (4), 732-737 (2011)
   PUBMED   21782797
  REMARK    GeneRIF: Interphase NuSAP-chromatin interaction suggests additional
            functions for NuSAP, as recently identified for other nuclear
            spindle assembly factors with a role in gene expression or DNA
            damage response.
REFERENCE   9  (bases 1 to 2263)
  AUTHORS   Wadia,P.P., Coram,M., Armstrong,R.J., Mindrinos,M., Butte,A.J. and
            Miklos,D.B.
  TITLE     Antibodies specifically target AML antigen NuSAP1 after allogeneic
            bone marrow transplantation
  JOURNAL   Blood 115 (10), 2077-2087 (2010)
   PUBMED   20053754
  REMARK    GeneRIF: NuSAP1 is recognized as an immunogenic antigen in 65% of
            patients with AML following allogeneic HCT and suggests a tumor
            antigen role.
REFERENCE   10 (bases 1 to 2263)
  AUTHORS   Raemaekers,T., Ribbeck,K., Beaudouin,J., Annaert,W., Van Camp,M.,
            Stockmans,I., Smets,N., Bouillon,R., Ellenberg,J. and Carmeliet,G.
  TITLE     NuSAP, a novel microtubule-associated protein involved in mitotic
            spindle organization
  JOURNAL   J Cell Biol 162 (6), 1017-1029 (2003)
   PUBMED   12963707
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB046574.1 and AF290612.1.
            
            On Nov 23, 2018 this sequence version replaced NM_016359.4.
            
            Summary: NUSAP1 is a nucleolar-spindle-associated protein that
            plays a role in spindle microtubule organization (Raemaekers et
            al., 2003 [PubMed 12963707]).[supplied by OMIM, Jun 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF290612.1, AF090915.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000559596.6/ ENSP00000453403.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 DB046574.1         154-162
            10-1472             AF290612.1         1-1463
            1473-2263           AF290612.1         1465-2255
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1"
     gene            1..2263
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /note="nucleolar and spindle associated protein 1"
                     /db_xref="GeneID:51203"
                     /db_xref="HGNC:HGNC:18538"
                     /db_xref="MIM:612818"
     exon            1..170
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..1403
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /note="isoform 1 is encoded by transcript variant 1;
                     nucleolar protein ANKT"
                     /codon_start=1
                     /product="nucleolar and spindle-associated protein 1
                     isoform 1"
                     /protein_id="NP_057443.2"
                     /db_xref="CCDS:CCDS45234.1"
                     /db_xref="GeneID:51203"
                     /db_xref="HGNC:HGNC:18538"
                     /db_xref="MIM:612818"
                     /translation="MIIPSLEELDSLKYSDLQNLAKSLGLRANLRATKLLKALKGYIK
                     HEARKGNENQDESQTSASSCDETEIQISNQEEAERQPLGHVTKTRRRCKTVRVDPDSQ
                     QNHSEIKISNPTEFQNHEKQESQDLRATAKVPSPPDEHQEAENAVSSGNRDSKVPSEG
                     KKSLYTDESSKPGKNKRTAITTPNFKKLHEAHFKEMESIDQYIERKKKHFEEHNSMNE
                     LKQQPINKGGVRTPVPPRGRLSVASTPISQRRSQGRSCGPASQSTLGLKGSLKRSAIS
                     AAKTGVRFSAATKDNEHKRSLTKTPARKSAHVTVSGGTPKGEAVLGTHKLKTITGNSA
                     AVITPFKLTTEATQTPVSNKKPVFDLKASLSRPLNYEPHKGKLKPWGQSKENNYLNQH
                     VNRINFYKKTYKQPHLQTKEEQRKKREQERKEKKAKVLGMRRGLILAED"
     exon            171..239
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            240..383
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            384..525
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            526..627
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            628..737
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            738..925
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1083
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1200
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1309
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..2263
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1993..1998
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /note="hexamer: AATAAA"
     polyA_site      2011
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /note="major polyA site"
     regulatory      2241..2246
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
                     /note="hexamer: AATAAA"
     polyA_site      2263
                     /gene="NUSAP1"
                     /gene_synonym="ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310;
                     SAPL"
ORIGIN      
        1 gtggcgccag ggatttgaac cgcgctgacg aagtttggtg atccatcttc cgagtatcgc
       61 cgggatttcg aatcgcgatg atcatcccct ctctagagga gctggactcc ctcaagtaca
      121 gtgacctgca gaacttagcc aagagtctgg gtctccgggc caacctgagg gcaaccaagt
      181 tgttaaaagc cttgaaaggc tacattaaac atgaggcaag aaaaggaaat gagaatcagg
      241 atgaaagtca aacttctgca tcctcttgtg atgagactga gatacagatc agcaaccagg
      301 aagaagctga gagacagcca cttggccatg tcaccaaaac aaggagaagg tgcaagactg
      361 tccgtgtgga ccctgactca cagcagaatc attcagagat aaaaataagt aatcccactg
      421 aattccagaa tcatgaaaag caggaaagcc aggatctcag agctactgca aaagttcctt
      481 ctccaccaga cgagcaccaa gaagctgaga atgctgtttc ctcaggtaac agagattcaa
      541 aggtaccttc agaaggaaag aaatctctct acacagatga gtcatccaaa cctggaaaaa
      601 ataaaagaac tgcaatcact actccaaact ttaagaagct tcatgaagct cattttaagg
      661 aaatggagtc cattgatcaa tatattgaga gaaaaaagaa acattttgaa gaacacaatt
      721 ccatgaatga actgaagcag cagcccatca ataagggagg ggtcaggact ccagtacctc
      781 caagaggaag actctctgtg gcttctactc ccatcagcca acgacgctcg caaggccggt
      841 cttgtggccc tgcaagtcag agtaccttgg gtctgaaggg gtcactcaag cgctctgcta
      901 tctctgcagc taaaacgggt gtcaggtttt cagctgctac taaagataat gagcataagc
      961 gttcactgac caagactcca gccagaaagt ctgcacatgt gaccgtgtct gggggcaccc
     1021 caaaaggcga ggctgtgctt gggacacaca aattaaagac catcacgggg aattctgctg
     1081 ctgttattac cccattcaag ttgacaactg aggcaacgca gactccagtc tccaataaga
     1141 aaccagtgtt tgatcttaaa gcaagtttgt ctcgtcccct caactatgaa ccacacaaag
     1201 gaaagctaaa accatggggg caatctaaag aaaataatta tctaaatcaa catgtcaaca
     1261 gaattaactt ctacaagaaa acttacaaac aaccccatct ccagacaaag gaagagcaac
     1321 ggaagaaacg cgagcaagaa cgaaaggaga agaaagcaaa ggttttggga atgcgaaggg
     1381 gcctcatttt ggctgaagat taataatttt ttaacatctt gtaaatattc ctgtattctc
     1441 aacttttttc cttttgtaaa tttttttttt ttgctgtcat ccccacttta gtcacgagat
     1501 ctttttctgc taactgttca tagtctgtgt agtgtccatg ggttcttcat gtgctatgat
     1561 ctctgaaaag acgttatcac cttaaagctc aaattctttg ggatggtttt tacttaagtc
     1621 cattaacaat tcaggtttct aacgagaccc atcctaaaat tctgtttcta gatttttaat
     1681 gtcaagttcc caagttcccc ctgctggttc taatattaac agaactgcag tcttctgcta
     1741 gccaatagca tttacctgat ggcagctagt tatgcaagct tcaggagaat ttgaacaata
     1801 acaagaatag ggtaagctgg gatagaaagg ccacctcttc actctctata gaatatagta
     1861 acctttatga aacggggcca tatagtttgg ttatgacatc aatattttac ctaggtgaaa
     1921 ttgtttaggc ttatgtacct tcgttcaaat atcctcatgt aattgccatc tgtcactcac
     1981 tatattcaca aaaataaaac tctacaactc attctaacat tgcttactta aaagctacat
     2041 agccctatcg aaatgcgagg attaatgctt taatgctttt agagacaggg tctcactgtg
     2101 ttgcccaggc tggtctcaaa ctccaccaaa tgtacttctt attcatttta tggaaaagac
     2161 taggctttgc ttagtatcat gtccatgttt ccttcacctc agtggagctt ctgagtttta
     2221 tactgctcaa gatcgtcata aataaaattt tttctcattg tca
//
