LOCUS       NM_138285               1545 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens nucleoporin 35 (NUP35), transcript variant 1, mRNA.
ACCESSION   NM_138285
VERSION     NM_138285.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1545)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 1545)
  AUTHORS   Schwartz,T.U.
  TITLE     The Structure Inventory of the Nuclear Pore Complex
  JOURNAL   J Mol Biol 428 (10 Pt A), 1986-2000 (2016)
   PUBMED   27016207
  REMARK    Review article
REFERENCE   5  (bases 1 to 1545)
  AUTHORS   Bui,K.H., von Appen,A., DiGuilio,A.L., Ori,A., Sparks,L.,
            Mackmull,M.T., Bock,T., Hagen,W., Andres-Pons,A., Glavy,J.S. and
            Beck,M.
  TITLE     Integrated structural analysis of the human nuclear pore complex
            scaffold
  JOURNAL   Cell 155 (6), 1233-1243 (2013)
   PUBMED   24315095
REFERENCE   6  (bases 1 to 1545)
  AUTHORS   Hawryluk-Gara,L.A., Platani,M., Santarella,R., Wozniak,R.W. and
            Mattaj,I.W.
  TITLE     Nup53 is required for nuclear envelope and nuclear pore complex
            assembly
  JOURNAL   Mol Biol Cell 19 (4), 1753-1762 (2008)
   PUBMED   18256286
  REMARK    GeneRIF: defined regions of Nup53 that bind to neighboring
            nucleoporins as well as those domains that target Nup53 to the
            nuclear pore complex.
REFERENCE   7  (bases 1 to 1545)
  AUTHORS   Beausoleil,S.A., Villen,J., Gerber,S.A., Rush,J. and Gygi,S.P.
  TITLE     A probability-based approach for high-throughput protein
            phosphorylation analysis and site localization
  JOURNAL   Nat Biotechnol 24 (10), 1285-1292 (2006)
   PUBMED   16964243
REFERENCE   8  (bases 1 to 1545)
  AUTHORS   Mansfeld,J., Guttinger,S., Hawryluk-Gara,L.A., Pante,N., Mall,M.,
            Galy,V., Haselmann,U., Muhlhausser,P., Wozniak,R.W., Mattaj,I.W.,
            Kutay,U. and Antonin,W.
  TITLE     The conserved transmembrane nucleoporin NDC1 is required for
            nuclear pore complex assembly in vertebrate cells
  JOURNAL   Mol Cell 22 (1), 93-103 (2006)
   PUBMED   16600873
REFERENCE   9  (bases 1 to 1545)
  AUTHORS   Hawryluk-Gara,L.A., Shibuya,E.K. and Wozniak,R.W.
  TITLE     Vertebrate Nup53 interacts with the nuclear lamina and is required
            for the assembly of a Nup93-containing complex
  JOURNAL   Mol Biol Cell 16 (5), 2382-2394 (2005)
   PUBMED   15703211
REFERENCE   10 (bases 1 to 1545)
  AUTHORS   Cronshaw,J.M., Krutchinsky,A.N., Zhang,W., Chait,B.T. and
            Matunis,M.J.
  TITLE     Proteomic analysis of the mammalian nuclear pore complex
  JOURNAL   J Cell Biol 158 (5), 915-927 (2002)
   PUBMED   12196509
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC061698.1 and FN117342.1.
            
            On Nov 22, 2018 this sequence version replaced NM_138285.4.
            
            Summary: This gene encodes a member of the nucleoporin family. The
            encoded protein contains two membrane binding regions, is localized
            to the nuclear rim, and is part of the nuclear pore complex. All
            molecules entering or leaving the nucleus either diffuse through or
            are actively transported by the nuclear pore complex. Alternative
            splicing results in multiple transcript variants. Pseudogenes of
            this gene have been defined on chromosomes 7 and 10. [provided by
            RefSeq, Dec 2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC022333.1, AF514993.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000295119.9/ ENSP00000295119.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1542              BC061698.1         43-1584
            1543-1545           FN117342.1         49-51
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q32.1"
     gene            1..1545
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /note="nucleoporin 35"
                     /db_xref="GeneID:129401"
                     /db_xref="HGNC:HGNC:29797"
                     /db_xref="MIM:608140"
     exon            1..55
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..996
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /note="isoform a is encoded by transcript variant 1;
                     mitotic phosphoprotein 44; nucleoporin NUP53; nuclear pore
                     complex protein Nup53; nucleoporin 35kDa"
                     /codon_start=1
                     /product="nucleoporin NUP35 isoform a"
                     /protein_id="NP_612142.2"
                     /db_xref="CCDS:CCDS2290.1"
                     /db_xref="GeneID:129401"
                     /db_xref="HGNC:HGNC:29797"
                     /db_xref="MIM:608140"
                     /translation="MAAFAVEPQGPALGSEPMMLGSPTSPKPGVNAQFLPGFLMGDLP
                     APVTPQPRSISGPSVGVMEMRSPLLAGGSPPQPVVPAHKDKSGAPPVRSIYDDISSPG
                     LGSTPLTSRRQPNISVMQSPLVGVTSTPGTGQSMFSPASIGQPRKTTLSPAQLDPFYT
                     QGDSLTSEDHLDDSWVTVFGFPQASASYILLQFAQYGNILKHVMSNTGNWMHIRYQSK
                     LQARKALSKDGRIFGESIMIGVKPCIDKSVMESSDRCALSSPSLAFTPPIKTLGTPTQ
                     PGSTPRISTMRPLATAYKASTSDYQVISDRQTPKKDESLVSKAMEYMFGW"
     exon            56..226
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /inference="alignment:Splign:2.1.0"
     exon            227..354
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /inference="alignment:Splign:2.1.0"
     exon            355..412
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /inference="alignment:Splign:2.1.0"
     exon            413..554
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /inference="alignment:Splign:2.1.0"
     exon            555..624
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /inference="alignment:Splign:2.1.0"
     exon            625..753
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /inference="alignment:Splign:2.1.0"
     exon            754..918
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1545
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1526..1531
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /note="hexamer: AATAAA"
     polyA_site      1545
                     /gene="NUP35"
                     /gene_synonym="MP-44; MP44; NP44; NUP53"
                     /note="major polyA site"
ORIGIN      
        1 gtagttgccg acgcaatggc agcctttgca gtggaacctc aggggcccgc gttaggatct
       61 gaaccaatga tgctgggttc acccacatct ccaaagccag gagttaatgc ccagttctta
      121 cctggatttt taatggggga tttgccagct ccggtgactc cacaacctcg atcaattagt
      181 ggcccttcag taggagtaat ggaaatgaga tcacctttac ttgcaggtgg gtcaccacca
      241 caaccagttg taccagctca taaagataaa agtggcgctc caccagttag aagtatatat
      301 gatgacattt ctagcccagg acttggatca acacctttaa cttcaagaag acagccaaac
      361 atttcagtaa tgcagagtcc tcttgttgga gttacatcta ctcctggaac agggcaaagt
      421 atgtttagtc cagcaagtat cggtcagcca cgaaagacga cattatctcc tgcccagttg
      481 gatccttttt atactcaagg agattctttg acttcagaag atcacctcga tgactcttgg
      541 gtgactgtat ttgggtttcc tcaagcatct gcttcctaca tattactaca atttgcacag
      601 tatgggaata tcttaaaaca tgtgatgtct aatacaggaa attggatgca tattcgttat
      661 caatctaaac tgcaggctcg gaaagcctta agcaaagatg ggaggatttt tggagaatcc
      721 atcatgattg gtgtaaaacc atgtattgac aaaagtgtta tggaaagcag tgacagatgt
      781 gctttatcat ctccatcttt agcctttaca ccaccaatca aaactctagg tacaccaaca
      841 caacctggaa gtactcctag gatttctacc atgagacctc ttgctacagc atacaaagcc
      901 tctactagtg attatcaggt tatttctgac agacaaacgc caaaaaaaga tgaaagtctt
      961 gtatccaaag caatggagta catgtttggc tggtagtaga acaccaagaa ggaggttgct
     1021 acactaaaac agagttagca gagtgctgct ggttccttcg gttagttata taactgttcc
     1081 tgcagtattg gatagctatc tcatacttct tttagaaaga agcctttttc attaaggata
     1141 caacctattt gtagctcgca ctttaaaaga tgcttgagat acattttaaa gaaaactaaa
     1201 aatccctgta aataggattt tgtgctttct gtaacagtgc atgcttcagc acagaaaact
     1261 cagcattgat tattgtaaat taaataactg aaattgtggt gagacgtcat agtcttcatg
     1321 agaacgtggg ggtgaatttc atgaagggga actatagtta tttctaccga cacaaatatt
     1381 ataattagca atttgaatta tggtctttta atttagatag tatttaatat tttaattatc
     1441 cttgtttgta tatgtcctgt cacagagtgt cctcttggtg tattctaaaa cgagcattct
     1501 tttaaaaaac ctaaagtttc ttgataataa acattgtcaa tgata
//
