LOCUS       NM_015354               5689 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens nucleoporin 188 (NUP188), mRNA.
ACCESSION   NM_015354 XM_497078
VERSION     NM_015354.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Muir,A.M., Cohen,J.L., Sheppard,S.E., Guttipatti,P., Lo,T.Y.,
            Weed,N., Doherty,D., DeMarzo,D., Fagerberg,C.R., Kjaersgaard,L.,
            Larsen,M.J., Rump,P., Lohner,K., Hirsch,Y., Zeevi,D.A.,
            Zackai,E.H., Bhoj,E., Song,Y. and Mefford,H.C.
  TITLE     Bi-allelic Loss-of-Function Variants in NUP188 Cause a Recognizable
            Syndrome Characterized by Neurologic, Ocular, and Cardiac
            Abnormalities
  JOURNAL   Am J Hum Genet 106 (5), 623-631 (2020)
   PUBMED   32275884
  REMARK    GeneRIF: results implicate bi-allelic loss-of-function NUP188
            variants in a recessive syndrome characterized by a distinct
            neurologic, ophthalmologic, and facial phenotype
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Vishnoi,N., Dhanasekeran,K., Chalfant,M., Surovstev,I., Khokha,M.K.
            and Lusk,C.P.
  TITLE     Differential turnover of Nup188 controls its levels at centrosomes
            and role in centriole duplication
  JOURNAL   J Cell Biol 219 (3) (2020)
   PUBMED   32211895
  REMARK    GeneRIF: Differential turnover of Nup188 controls its levels at
            centrosomes and role in centriole duplication.
REFERENCE   3  (bases 1 to 5689)
  AUTHORS   Roczniak-Ferguson,A. and Ferguson,S.M.
  TITLE     Pleiotropic requirements for human TDP-43 in the regulation of cell
            and organelle homeostasis
  JOURNAL   Life Sci Alliance 2 (5), e201900358 (2019)
   PUBMED   31527135
  REMARK    GeneRIF: Results identified Nup188 as a novel TDP-43 target which
            controls the mRNA splicing of Nup188.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5689)
  AUTHORS   Del Viso,F., Huang,F., Myers,J., Chalfant,M., Zhang,Y., Reza,N.,
            Bewersdorf,J., Lusk,C.P. and Khokha,M.K.
  TITLE     Congenital Heart Disease Genetics Uncovers Context-Dependent
            Organization and Function of Nucleoporins at Cilia
  JOURNAL   Dev Cell 38 (5), 478-492 (2016)
   PUBMED   27593162
  REMARK    GeneRIF: we show that knockdown of Nup188 or its binding partner
            Nup93 leads to a loss of cilia during embryonic development while
            leaving NPC function largely intact. Many data, including the
            localization of endogenous Nup188/93 at cilia bases, support their
            direct role at cilia. Super-resolution imaging of Nup188 shows two
            barrel-like structures
REFERENCE   5  (bases 1 to 5689)
  AUTHORS   Schwartz,T.U.
  TITLE     The Structure Inventory of the Nuclear Pore Complex
  JOURNAL   J Mol Biol 428 (10 Pt A), 1986-2000 (2016)
   PUBMED   27016207
  REMARK    Review article
REFERENCE   6  (bases 1 to 5689)
  AUTHORS   Braun,D.A., Sadowski,C.E., Kohl,S., Lovric,S., Astrinidis,S.A.,
            Pabst,W.L., Gee,H.Y., Ashraf,S., Lawson,J.A., Shril,S., Airik,M.,
            Tan,W., Schapiro,D., Rao,J., Choi,W.I., Hermle,T., Kemper,M.J.,
            Pohl,M., Ozaltin,F., Konrad,M., Bogdanovic,R., Buscher,R.,
            Helmchen,U., Serdaroglu,E., Lifton,R.P., Antonin,W. and
            Hildebrandt,F.
  TITLE     Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause
            steroid-resistant nephrotic syndrome
  JOURNAL   Nat Genet 48 (4), 457-465 (2016)
   PUBMED   26878725
REFERENCE   7  (bases 1 to 5689)
  AUTHORS   Bui,K.H., von Appen,A., DiGuilio,A.L., Ori,A., Sparks,L.,
            Mackmull,M.T., Bock,T., Hagen,W., Andres-Pons,A., Glavy,J.S. and
            Beck,M.
  TITLE     Integrated structural analysis of the human nuclear pore complex
            scaffold
  JOURNAL   Cell 155 (6), 1233-1243 (2013)
   PUBMED   24315095
REFERENCE   8  (bases 1 to 5689)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   9  (bases 1 to 5689)
  AUTHORS   Denoeud,F., Kapranov,P., Ucla,C., Frankish,A., Castelo,R.,
            Drenkow,J., Lagarde,J., Alioto,T., Manzano,C., Chrast,J., Dike,S.,
            Wyss,C., Henrichsen,C.N., Holroyd,N., Dickson,M.C., Taylor,R.,
            Hance,Z., Foissac,S., Myers,R.M., Rogers,J., Hubbard,T., Harrow,J.,
            Guigo,R., Gingeras,T.R., Antonarakis,S.E. and Reymond,A.
  TITLE     Prominent use of distal 5' transcription start sites and discovery
            of a large number of additional exons in ENCODE regions
  JOURNAL   Genome Res 17 (6), 746-759 (2007)
   PUBMED   17567994
REFERENCE   10 (bases 1 to 5689)
  AUTHORS   Miller,B.R., Powers,M., Park,M., Fischer,W. and Forbes,D.J.
  TITLE     Identification of a new vertebrate nucleoporin, Nup188, with the
            use of a novel organelle trap assay
  JOURNAL   Mol Biol Cell 11 (10), 3381-3396 (2000)
   PUBMED   11029043
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY094051.1, D79991.1 and
            AA731883.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015354.2.
            
            Summary: The nuclear pore complex (NPC) is found on the nuclear
            envelope and forms a gateway that regulates the flow of proteins
            and RNAs between the cytoplasm and nucleoplasm. The NPC is
            comprised of approximately 30 distinct proteins collectively known
            as nucleoporins. Nucleoporins are pore-complex-specific
            glycoproteins which often have cytoplasmically oriented O-linked
            N-acetylglucosamine residues and numerous repeats of the
            pentapeptide sequence XFXFG. However, the nucleoporin protein
            encoded by this gene does not contain the typical FG repeat
            sequences found in most vertebrate nucleoporins. This nucleoporin
            is thought to form part of the scaffold for the central channel of
            the nuclear pore. [provided by RefSeq, Jan 2013].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.225471.1,
                                           SRR1660809.113796.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372577.2/ ENSP00000361658.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                HY094051.1         17-48
            33-5688             D79991.1           1-5656
            5689-5689           AA731883.1         41-41               c
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.11"
     gene            1..5689
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /note="nucleoporin 188"
                     /db_xref="GeneID:23511"
                     /db_xref="HGNC:HGNC:17859"
                     /db_xref="MIM:615587"
     exon            1..53
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..5271
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /note="nucleoporin NUP188 homolog; nucleoporin 188kDa"
                     /codon_start=1
                     /product="nucleoporin NUP188"
                     /protein_id="NP_056169.1"
                     /db_xref="CCDS:CCDS35156.1"
                     /db_xref="GeneID:23511"
                     /db_xref="HGNC:HGNC:17859"
                     /db_xref="MIM:615587"
                     /translation="MAAAAGGPCVRSSRELWTILLGRSALRELSQIEAELNKHWRRLL
                     EGLSYYKPPSPSSAEKVKANKDVASPLKELGLRISKFLGLDEEQSVQLLQCYLQEDYR
                     GTRDSVKTVLQDERQSQALILKIADYYYEERTCILRCVLHLLTYFQDERHPYRVEYAD
                     CVDKLEKELVSKYRQQFEELYKTEAPTWETHGNLMTERQVSRWFVQCLREQSMLLEII
                     FLYYAYFEMAPSDLLVLTKMFKEQGFGSRQTNRHLVDETMDPFVDRIGYFSALILVEG
                     MDIESLHKCALDDRRELHQFAQDGLICQDMDCLMLTFGDIPHHAPVLLAWALLRHTLN
                     PEETSSVVRKIGGTAIQLNVFQYLTRLLQSLASGGNDCTTSTACMCVYGLLSFVLTSL
                     ELHTLGNQQDIIDTACEVLADPSLPELFWGTEPTSGLGIILDSVCGMFPHLLSPLLQL
                     LRALVSGKSTAKKVYSFLDKMSFYNELYKHKPHDVISHEDGTLWRRQTPKLLYPLGGQ
                     TNLRIPQGTVGQVMLDDRAYLVRWEYSYSSWTLFTCEIEMLLHVVSTADVIQHCQRVK
                     PIIDLVHKVISTDLSIADCLLPITSRIYMLLQRLTTVISPPVDVIASCVNCLTVLAAR
                     NPAKVWTDLRHTGFLPFVAHPVSSLSQMISAEGMNAGGYGNLLMNSEQPQGEYGVTIA
                     FLRLITTLVKGQLGSTQSQGLVPCVMFVLKEMLPSYHKWRYNSHGVREQIGCLILELI
                     HAILNLCHETDLHSSHTPSLQFLCICSLAYTEAGQTVINIMGIGVDTIDMVMAAQPRS
                     DGAEGQGQGQLLIKTVKLAFSVTNNVIRLKPPSNVVSPLEQALSQHGAHGNNLIAVLA
                     KYIYHKHDPALPRLAIQLLKRLATVAPMSVYACLGNDAAAIRDAFLTRLQSKIEDMRI
                     KVMILEFLTVAVETQPGLIELFLNLEVKDGSDGSKEFSLGMWSCLHAVLELIDSQQQD
                     RYWCPPLLHRAAIAFLHALWQDRRDSAMLVLRTKPKFWENLTSPLFGTLSPPSETSEP
                     SILETCALIMKIICLEIYYVVKGSLDQSLKDTLKKFSIEKRFAYWSGYVKSLAVHVAE
                     TEGSSCTSLLEYQMLVSAWRMLLIIATTHADIMHLTDSVVRRQLFLDVLDGTKALLLV
                     PASVNCLRLGSMKCTLLLILLRQWKRELGSVDEILGPLTEILEGVLQADQQLMEKTKA
                     KVFSAFITVLQMKEMKVSDIPQYSQLVLNVCETLQEEVIALFDQTRHSLALGSATEDK
                     DSMETDDCSRSRHRDQRDGVCVLGLHLAKELCEVDEDGDSWLQVTRRLPILPTLLTTL
                     EVSLRMKQNLHFTEATLHLLLTLARTQQGATAVAGAGITQSICLPLLSVYQLSTNGTA
                     QTPSASRKSLDAPSWPGVYRLSMSLMEQLLKTLRYNFLPEALDFVGVHQERTLQCLNA
                     VRTVQSLACLEEADHTVGFILQLSNFMKEWHFHLPQLMRDIQVNLGYLCQACTSLLHS
                     RKMLQHYLQNKNGDGLPSAVAQRVQRPPSAASAAPSSSKQPAADTEASEQQALHTVQY
                     GLLKILSKTLAALRHFTPDVCQILLDQSLDLAEYNFLFALSFTTPTFDSEVAPSFGTL
                     LATVNVALNMLGELDKKKEPLTQAVGLSTQAEGTRTLKSLLMFTMENCFYLLISQAMR
                     YLRDPAVHPRDKQRMKQELSSELSTLLSSLSRYFRRGAPSSPATGVLPSPQGKSTSLS
                     KASPESQEPLIQLVQAFVRHMQR"
     exon            54..108
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            109..182
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            183..267
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            268..348
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            349..393
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            394..486
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            487..606
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            607..818
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            819..933
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1134
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1224
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1290
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1410
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1537
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1690
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1817
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..1905
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1906..1982
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2097
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2218
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2219..2285
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2286..2414
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2415..2554
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2555..2661
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2662..2868
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2869..3038
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3039..3108
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3109..3176
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3177..3372
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3373..3450
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3451..3536
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3537..3682
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3683..3864
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3865..4065
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4066..4158
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4159..4317
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4318..4455
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4456..4530
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4531..4758
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4759..4890
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4891..4964
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4965..5094
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     exon            5095..5689
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5668..5673
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /note="hexamer: ATTAAA"
     polyA_site      5689
                     /gene="NUP188"
                     /gene_synonym="hNup188; KIAA0169; SANDSTEF"
                     /note="major polyA site"
ORIGIN      
        1 agggcgagcg ggcgcgcgaa gatggcggcg gccgccggcg ggccgtgtgt gaggagcagt
       61 agagaactgt ggactattct gcttggaagg tcagctctga gagagctgag tcagattgag
      121 gcagaactga ataaacattg gcggcgattg ttagaggggc tttcttacta caaacctccc
      181 agtccaagtt cagctgaaaa agtgaaagct aataaagatg tagcttcacc attgaaggaa
      241 ctgggtttaa gaatcagcaa gtttttgggt cttgatgaag aacagagtgt gcagttactc
      301 cagtgttacc tgcaagagga ctacaggggt actcgggact cagtaaagac agtactgcaa
      361 gatgagaggc agagccaggc cttaatcctg aagattgcag attattatta tgaagaaaga
      421 acctgtattc ttcgttgtgt cttacacctt ctcacttact tccaagatga aagacacccc
      481 tatagggttg aatatgcaga ctgtgttgat aaattggaga aggaactagt ttcaaaatac
      541 agacagcagt tcgaagagct ttataaaact gaagcaccaa cttgggagac acatggaaat
      601 ctcatgacag agcgccaagt gtctcgctgg tttgttcagt gccttcggga acagtccatg
      661 ctgctagaaa ttattttcct ttattatgca tactttgaga tggcacccag tgacttactt
      721 gtattaacca agatgtttaa agagcaagga tttggtagta ggcagaccaa taggcacctg
      781 gtggatgaga ctatggatcc ttttgtagat cggattggct acttcagtgc cctcatcctg
      841 gtggagggca tggatatcga gtccttgcat aagtgtgctt tggatgacag aagagaactg
      901 catcagtttg cgcaggatgg gcttatttgt caggatatgg actgtttaat gttgaccttt
      961 ggggacattc cacatcatgc cccagtgctt ttggcctggg ctctcctccg tcacactctg
     1021 aacccagaag agacaagcag tgtggtccgg aagataggtg gcacagccat ccagctgaat
     1081 gtgtttcagt acttgacccg attgctccag tcccttgcca gtgggggaaa tgattgcacc
     1141 accagcactg catgcatgtg tgtctatgga ctgctctctt tcgttctgac ctcgttggag
     1201 ctgcacaccc tgggcaatca gcaggatata attgatacag catgtgaagt attggccgac
     1261 ccttctcttc cggaactgtt ctggggaaca gagccaactt ctggccttgg gatcattctg
     1321 gacagtgtgt gtggaatgtt tccccacctt ctctccccac tcctgcaact gctccgagcc
     1381 ctggtatcag ggaagtccac agccaaaaag gtgtatagct tcttggataa gatgtctttc
     1441 tacaatgaac tttataaaca caagcctcat gatgtgatct cccatgaaga tggaactctt
     1501 tggcggagac aaacacccaa actcctttat ccccttgggg gtcaaaccaa ccttcgcata
     1561 cctcaaggca ctgtgggcca agtaatgttg gatgataggg catacctggt acgctgggaa
     1621 tactcctata gcagctggac cctctttacc tgcgagattg aaatgttgct tcatgttgtt
     1681 tcaactgcag atgtgattca gcactgccag cgagtcaaac ccatcattga tctcgtccat
     1741 aaggtcatca gtacagacct gtcgatagca gactgtctcc tgcccatcac atctcgcatc
     1801 tacatgctgc tgcagcggtt aacgacagtg atctccccac ctgtggatgt cattgcttct
     1861 tgtgtcaact gcttaactgt tttggctgcc cgcaatccag caaaggtctg gactgatctt
     1921 cgtcacacag gttttttacc atttgtggcc catcctgtct ccagcctgag tcagatgatt
     1981 agtgcggaag ggatgaatgc tggagggtac ggaaacctct tgatgaacag tgaacagcct
     2041 cagggcgagt atggggttac tattgccttt ctgcgcttga tcaccaccct tgtcaagggg
     2101 caacttggta gtacccagag ccaaggactt gtaccctgtg taatgtttgt gctgaaggag
     2161 atgcttccca gctaccataa gtggcgctac aactctcatg gagtgaggga acagattggt
     2221 tgcctgatct tggagctgat tcatgcgata ctgaacctgt gccacgagac agacctgcac
     2281 agcagtcata ctcccagcct gcagtttctc tgcatctgca gcctggcata cacagaagca
     2341 ggacagacag ttatcaatat catgggcatt ggcgtggaca ccattgacat ggtgatggct
     2401 gctcagcctc gaagtgatgg ggcagagggc caggggcagg gccagctgct gatcaagaca
     2461 gtgaaactgg cattctccgt caccaacaat gttattcggc tgaaacctcc ttctaatgtg
     2521 gtgtcccccc tggaacaggc tctctcacaa catggtgctc atggaaacaa cctcattgct
     2581 gttctagcca aatacatcta ccacaaacat gaccctgctt tgccacgtct tgccattcag
     2641 ctgctgaaac gtctggccac ggtggcccca atgtcagtgt atgcttgtct gggcaatgat
     2701 gcggctgcca ttcgtgatgc cttcctgacc cgattgcaga gcaaaattga ggacatgcgc
     2761 atcaaagtca tgattctaga gttcctcact gttgcagtag agacccagcc aggcctcatc
     2821 gaactgtttc tgaacctgga agttaaggat ggcagtgatg gctcaaagga attcagcctt
     2881 gggatgtgga gctgtctcca tgcagtgctg gagctgattg attcccaaca gcaagatcga
     2941 tactggtgcc cacccctgct gcatcgtgcc gccattgcct ttttgcatgc tctgtggcag
     3001 gatcggaggg acagtgccat gctggtcctc cgaaccaaac ccaagttttg ggaaaattta
     3061 accagtccgc tgtttggaac cctttctcct ccctctgaaa catcagagcc cagcatcctg
     3121 gaaacctgtg ccctaatcat gaagataatt tgcttggaga tatactatgt agtaaagggt
     3181 tcattagacc agtcattaaa ggatacactg aagaaatttt ccatcgagaa acgctttgcc
     3241 tactggtcag ggtatgtcaa gtcattggca gttcacgtgg ccgaaacaga aggcagcagc
     3301 tgcacctcct tgttagagta ccagatgctg gtgtccgcct ggaggatgct tctcatcatt
     3361 gccaccactc atgcagatat aatgcacctg actgactctg tggtgcgtcg ccagctcttt
     3421 cttgacgtgc ttgatggaac caaagcatta ctcctagttc cagcctcagt gaactgcctt
     3481 cgccttggct ccatgaagtg cactctgctg cttatcctcc tccggcagtg gaagagagag
     3541 ttaggttctg tggatgaaat ccttggaccc ttgacggaga tcctggaggg agtgctgcag
     3601 gccgaccagc aactcatgga gaagaccaag gccaaggtgt tctcagcatt catcacagtg
     3661 ttgcaaatga aggagatgaa agtaagtgac atcccccagt actcccagct ggtgctgaat
     3721 gtctgtgaga ccctccaaga ggaagtgatt gcactcttcg accagacccg ccacagtctg
     3781 gcattaggca gtgccacaga ggacaaggac agcatggaga ctgacgactg ttctcggtcc
     3841 cggcacaggg accagcgtga tggggtgtgt gtcctgggcc tgcacctggc caaggagctg
     3901 tgtgaggtag acgaggatgg tgactcctgg ctgcaggtaa cccgcaggct ccccatccta
     3961 cccaccctcc tcaccactct agaggtgagc cttcgcatga agcagaacct gcatttcact
     4021 gaggccacat tgcatctgct cctcaccctg gctcgcactc agcagggagc cacagcagtg
     4081 gctggagctg gcatcaccca gagcatttgt ttgccccttc tgagtgtgta ccagctgagc
     4141 accaacggca cagcacagac acctagtgcc tctcggaagt ccctggatgc cccctcttgg
     4201 ccaggagtct accgcctgtc catgtccctg atggagcagc tgctcaaaac tctgcgctac
     4261 aacttcctgc ctgaggccct ggacttcgtg ggtgtccacc aggagcggac cttacagtgc
     4321 ctcaacgcag tgaggacagt gcagagtctg gcctgcctgg aggaggcgga ccacaccgtg
     4381 ggttttattc tgcagctctc taacttcatg aaggagtggc acttccacct gcctcagctc
     4441 atgcgtgata tccaggtcaa cctgggttac ttgtgccagg catgtacctc tctcctgcac
     4501 agtcgaaaga tgctgcagca ttacttacag aacaaaaatg gggatggcct cccctcagct
     4561 gttgcccagc gagtccagag gccaccgtct gctgcttctg ctgccccctc ctcctcaaag
     4621 cagcccgctg ctgacacaga ggcatcagag cagcaggcct tgcacacagt ccagtatggc
     4681 cttctcaaga tcctcagcaa gacgctggca gccctgcgcc acttcacccc agatgtctgc
     4741 cagattctgc tggatcagtc cctggacctt gctgaataca acttcctgtt tgccctgagc
     4801 tttaccactc ccacctttga ctccgaagtg gccccctcct tcgggaccct tctggccaca
     4861 gtgaatgtgg ccctcaacat gcttggagag ctggacaaga aaaaggagcc cctcacccag
     4921 gcagtggggc tcagcacaca ggcagaaggg accaggacgt taaagtccct cctgatgttt
     4981 accatggaaa actgcttcta cctgctcatc tctcaggcga tgcggtacct tagggacccg
     5041 gctgtgcacc cccgggacaa acagcggatg aagcaggagc tcagctctga gttgagcacg
     5101 ctgctgtcca gcctctcgcg ctacttccgc cggggagccc ccagctcccc tgccactggt
     5161 gtcctcccct cgccgcaggg caagtccacc tctctctcca aagccagccc tgagagtcag
     5221 gagcctctga tccagttggt gcaggcgttt gtccggcata tgcaaagata gggcagtgct
     5281 gttctgccca cctacccctc tccaccagcc tacactgcac cctggctggc aggggtgctg
     5341 ctggctgcta gggcctatac aatggagggc acctcctgtc acccccctcc cggagtagcc
     5401 acgactccag ccaccaccca ctgacgttat ttttatacta gatgaagagg tcaacagcag
     5461 gcatggggag ccgagtcttc tgtgctcagg tcctcacgct gcagacgccc cctagaggaa
     5521 ctttccttcc tttccagcat tccccacagc actgccggcc aggggagagg cggcagccca
     5581 gcagagggct ctatgcacgg gtttcaaacc tgttttccac actctgtctt tgcagttttg
     5641 gtaattctgt ggtctattta tacagatatt aaaatcttgt ttatagaca
//
