LOCUS       NM_001005743            3604 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens NUMB endocytic adaptor protein (NUMB), transcript
            variant 1, mRNA.
ACCESSION   NM_001005743
VERSION     NM_001005743.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Kliche,J., Garvanska,D.H., Simonetti,L., Badgujar,D.,
            Dobritzsch,D., Nilsson,J., Davey,N.E. and Ivarsson,Y.
  TITLE     Large-scale phosphomimetic screening identifies phospho-modulated
            motif-based protein interactions
  JOURNAL   Mol Syst Biol 19 (7), e11164 (2023)
   PUBMED   37219487
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   He,J., Yang,L., Chang,P., Yang,S., Wang,Y., Lin,S., Tang,Q. and
            Zhang,Y.
  TITLE     Zika Virus Induces Degradation of the Numb Protein Required through
            Embryonic Neurogenesis
  JOURNAL   Viruses 15 (6), 1258 (2023)
   PUBMED   37376558
  REMARK    GeneRIF: Zika Virus Induces Degradation of the Numb Protein
            Required through Embryonic Neurogenesis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3604)
  AUTHORS   Filippone,M.G., Freddi,S., Zecchini,S., Restelli,S., Colaluca,I.N.,
            Bertalot,G., Pece,S., Tosoni,D. and Di Fiore,P.P.
  TITLE     Aberrant phosphorylation inactivates Numb in breast cancer causing
            expansion of the stem cell pool
  JOURNAL   J Cell Biol 221 (12) (2022)
   PUBMED   36200956
  REMARK    GeneRIF: Aberrant phosphorylation inactivates Numb in breast cancer
            causing expansion of the stem cell pool.
REFERENCE   4  (bases 1 to 3604)
  AUTHORS   Bubak,M.P., Stout,K., Tomtschik,J., Peterson,E., Cardozo,C.P.,
            Graham,Z.A. and Gallagher,P.
  TITLE     Notch, Numb and Numb-like responses to exercise-induced muscle
            damage in human skeletal muscle
  JOURNAL   Exp Physiol 107 (8), 800-806 (2022)
   PUBMED   35562322
  REMARK    GeneRIF: Notch, Numb and Numb-like responses to exercise-induced
            muscle damage in human skeletal muscle.
REFERENCE   5  (bases 1 to 3604)
  AUTHORS   Qin,X. and Cao,Y.
  TITLE     Prognostic Biomarker NUMB Is Inhibited by Breast Cancer Cell
            Exosomes to Promote Breast Cancer Progression
  JOURNAL   J Immunol Res 2022, 6032076 (2022)
   PUBMED   35478938
  REMARK    GeneRIF: Prognostic Biomarker NUMB Is Inhibited by Breast Cancer
            Cell Exosomes to Promote Breast Cancer Progression.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3604)
  AUTHORS   Dho,S.E., Jacob,S., Wolting,C.D., French,M.B., Rohrschneider,L.R.
            and McGlade,C.J.
  TITLE     The mammalian numb phosphotyrosine-binding domain. Characterization
            of binding specificity and identification of a novel PDZ
            domain-containing numb binding protein, LNX
  JOURNAL   J Biol Chem 273 (15), 9179-9187 (1998)
   PUBMED   9535908
REFERENCE   7  (bases 1 to 3604)
  AUTHORS   Salcini,A.E., Confalonieri,S., Doria,M., Santolini,E., Tassi,E.,
            Minenkova,O., Cesareni,G., Pelicci,P.G. and Di Fiore,P.P.
  TITLE     Binding specificity and in vivo targets of the EH domain, a novel
            protein-protein interaction module
  JOURNAL   Genes Dev 11 (17), 2239-2249 (1997)
   PUBMED   9303539
REFERENCE   8  (bases 1 to 3604)
  AUTHORS   Zhong,W., Feder,J.N., Jiang,M.M., Jan,L.Y. and Jan,Y.N.
  TITLE     Asymmetric localization of a mammalian numb homolog during mouse
            cortical neurogenesis
  JOURNAL   Neuron 17 (1), 43-53 (1996)
   PUBMED   8755477
REFERENCE   9  (bases 1 to 3604)
  AUTHORS   Wong,W.T., Schumacher,C., Salcini,A.E., Romano,A., Castagnino,P.,
            Pelicci,P.G. and Di Fiore,P.P.
  TITLE     A protein-binding domain, EH, identified in the receptor tyrosine
            kinase substrate Eps15 and conserved in evolution
  JOURNAL   Proc Natl Acad Sci U S A 92 (21), 9530-9534 (1995)
   PUBMED   7568168
REFERENCE   10 (bases 1 to 3604)
  AUTHORS   Sherrington,R., Rogaev,E.I., Liang,Y., Rogaeva,E.A., Levesque,G.,
            Ikeda,M., Chi,H., Lin,C., Li,G., Holman,K., Tsuda,T., Mar,L.,
            Foncin,J.F., Bruni,A.C., Montesi,M.P., Sorbi,S., Rainero,I.,
            Pinessi,L., Nee,L., Chumakov,I., Pollen,D., Brookes,A., Sanseau,P.,
            Polinsky,R.J., Wasco,W., Da Silva,H.A., Haines,J.L.,
            Perkicak-Vance,M.A., Tanzi,R.E., Roses,A.D., Fraser,P.E.,
            Rommens,J.M. and St George-Hyslop,P.H.
  TITLE     Cloning of a gene bearing missense mutations in early-onset
            familial Alzheimer's disease
  JOURNAL   Nature 375 (6534), 754-760 (1995)
   PUBMED   7596406
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC068476.1, AF171938.1 and
            AA872908.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 13, 2020 this sequence version replaced NM_001005743.1.
            
            Summary: The protein encoded by this gene plays a role in the
            determination of cell fates during development. The encoded
            protein, whose degradation is induced in a proteasome-dependent
            manner by MDM2, is a membrane-bound protein that has been shown to
            associate with EPS15, LNX1, and NOTCH1. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Feb
            2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF171938.1, SRR14038195.1519359.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000555238.6/ ENSP00000451300.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-166               BC068476.1         35-200
            167-3584            AF171938.1         151-3568
            3585-3604           AA872908.1         10-29               c
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q24.2-q24.3"
     gene            1..3604
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /note="NUMB endocytic adaptor protein"
                     /db_xref="GeneID:8650"
                     /db_xref="HGNC:HGNC:8060"
                     /db_xref="MIM:603728"
     exon            1..54
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     exon            55..186
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     exon            187..271
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     exon            272..412
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     CDS             287..2242
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein numb homolog; h-Numb"
                     /codon_start=1
                     /product="protein numb homolog isoform 1"
                     /protein_id="NP_001005743.1"
                     /db_xref="CCDS:CCDS32116.1"
                     /db_xref="GeneID:8650"
                     /db_xref="HGNC:HGNC:8060"
                     /db_xref="MIM:603728"
                     /translation="MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYL
                     GHVEVDESRGMHICEDAVKRLKAERKFFKGFFGKTGKKAVKAVLWVSADGLRVVDEKT
                     KDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTGERLSHAVGC
                     AFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQMQDAKK
                     AETDKIVVGSSVAPGNTAPSPSSPTSPTSDATTSLEMNNPHAIPRRHAPIEQLARQGS
                     FRGFPALSQKMSPFKRQLSLRINELPSTMQRKTDFPIKNAVPEVEGEAESISSLCSQI
                     TNAFSTPEDPFSSAPMTKPVTVVAPQSPTFQANGTDSAFHVLAKPAHTALAPVAMPVR
                     ETNPWAHAPDAANKEIAATCSGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKS
                     VRAQQPQASAAPLQPVLQPPPPTAISQPASPFQGNAFLTSQPVPVGVVPALQPAFVPA
                     QSYPVANGMPYPAPNVPVVGITPSQMVANVFGTAGHPQAAHPHQSPSLVRQQTFPHYE
                     ASSATTSPFFKPPAQHLNGSAAFNGVDDGRLASADRHTEVPTGTCPVDPFEAQWAALE
                     NKSKQRTNPSPTNPFSSDLQKTFEIEL"
     exon            413..487
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     exon            488..520
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     exon            521..595
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     exon            596..736
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     exon            737..941
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1235
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1382
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1526
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..3604
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3585..3590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /note="hexamer: AATAAA"
     polyA_site      3604
                     /gene="NUMB"
                     /gene_synonym="C14orf41; c14_5527; S171"
                     /note="major polyA site"
ORIGIN      
        1 actggcggcc gaggtagagg cagtggcgct tgagttggtc gggggcagcg gcagatttga
       61 ggcttaagca acttcttccg gggaagagtg ccagtgcagc cactgttaca attcaagatc
      121 ttgatctata tccatagatt ggaatattgg tgggccagca atcctcagac gcctcactta
      181 ggacaaatga ggaaactgag gcttggtgaa gttacgaaac ttgtccaaaa tcacacaact
      241 tgtaaagggc acagccaaga ttcagagcca ggctgtaaaa attaaaatga acaaattacg
      301 gcaaagtttt aggagaaaga aggatgttta tgttccagag gccagtcgtc cacatcagtg
      361 gcagacagat gaagaaggcg ttcgcaccgg aaaatgtagc ttcccggtta agtaccttgg
      421 ccatgtagaa gttgatgaat caagaggaat gcacatctgt gaagatgctg taaaaagatt
      481 gaaagctgaa aggaagttct tcaaaggctt ctttggaaaa actggaaaga aagcagttaa
      541 agcagttctg tgggtctcag cagatggact cagagttgtg gatgaaaaaa ctaaggacct
      601 catagttgac cagacgatag agaaagtttc tttctgtgcc ccagacagga actttgatag
      661 agccttttct tacatatgcc gtgatggcac cactcgtcgc tggatctgtc actgcttcat
      721 ggctgtcaag gacacaggtg aaaggttgag ccatgcagta ggctgtgctt ttgcagcctg
      781 tttagagcgc aagcagaagc gggagaagga atgtggagtg actgctactt ttgatgctag
      841 tcggaccact tttacaagag aaggatcatt ccgtgtcaca acagccactg aacaagcaga
      901 aagagaggag atcatgaaac aaatgcaaga tgccaagaaa gctgaaacag ataagatagt
      961 cgttggttca tcagttgccc ctggcaacac tgccccatcc ccatcctctc ccacctctcc
     1021 tacttctgat gccacgacct ctctggagat gaacaatcct catgccatcc cacgccggca
     1081 tgctccaatt gaacagcttg ctcgccaagg ctctttccga ggttttcctg ctcttagcca
     1141 gaagatgtca ccctttaaac gccaactatc cctacgcatc aatgagttgc cttccactat
     1201 gcagaggaag actgatttcc ccattaaaaa tgcagtgcca gaagtagaag gggaggcaga
     1261 gagcatcagc tccctgtgct cacagatcac caatgccttc agcacacctg aggacccctt
     1321 ctcatctgct ccgatgacca aaccagtgac agtggtggca ccacaatctc ctaccttcca
     1381 agctaatggc actgactcag ccttccatgt gcttgctaag ccagcccata ctgctctagc
     1441 acccgtagca atgcctgtgc gtgaaaccaa cccttgggcc catgcccctg atgctgctaa
     1501 caaggaaatt gcagccacat gttcggggac cgagtggggt caatcttctg gtgctgcctc
     1561 tccaggtctc ttccaggccg gtcatagacg tactccctct gaggccgacc gatggttaga
     1621 agaggtgtct aagagcgtcc gggctcagca gccccaggcc tcagctgctc ctctgcagcc
     1681 agttctccag cctcctccac ccactgccat ctcccagcca gcatcacctt tccaagggaa
     1741 tgcattcctc acctctcagc ctgtgccagt gggtgtggtc ccagccctgc aaccagcctt
     1801 tgtccctgcc cagtcctatc ctgtggccaa tggaatgccc tatccagccc ctaatgtgcc
     1861 tgtggtgggc atcactccct cccagatggt ggccaacgta tttggcactg caggccaccc
     1921 tcaggctgcc catccccatc agtcacccag cctggtcagg cagcagacat tccctcacta
     1981 cgaggcaagc agtgctacca ccagtccctt ctttaagcct cctgctcagc acctcaacgg
     2041 ttctgcagct ttcaatggtg tagatgatgg caggttggcc tcagcagaca ggcatacaga
     2101 ggttcctaca ggcacctgcc cagtggatcc ttttgaagcc cagtgggctg cattagaaaa
     2161 taagtccaag cagcgtacta atccctcccc taccaaccct ttctccagtg acttacagaa
     2221 gacgtttgaa attgaacttt aagcaatcat tatggctatg tatcttgtcc ataccagaca
     2281 gggagcaggg ggtagcggtc aaaggagcaa aacagacttt gtctcctgat tagtactctt
     2341 ttcactaatc ccaaaggtcc caaggaacaa gtccaggccc agagtactgt gaggggtgat
     2401 tttgaaagac atgggaaaaa gcattcctag agaaaagctg ccttgcaatt aggctaaaga
     2461 agtcaaggaa atgttgcttt ctgtactccc tcttccctta cccccttaca aatctctggc
     2521 aacagagagg caaagtatct gaacaagaat ctatattcca agcacattta ctgaaatgta
     2581 aaacacaaca ggaagcaaag caatctccct ttgtttttca ggccattcac ctgcctcctg
     2641 tcagtagtgg cctgtattag agatcaagaa gagtggtttg tgctcaggct ggggaacaga
     2701 gaggcacgct atgctgccag aattcccagg agggcatatc agcaactgcc cagcagagct
     2761 atattttggg ggagaagttg agcttccatt ttgagtaaca gaataaatat tatatatatc
     2821 aaaagccaaa atctttattt ttatgcattt agaatatttt aaatagttct cagatattaa
     2881 gaagttgtat gagttgtaag taatcttgcc aaaggtaaag gggctagttg taagaaattg
     2941 tacataagat tgatttatca ttgatgccta ctgaaataaa aagaggaaag gctggaagct
     3001 gcagacagga tccctagctt gttttctgtc agtcattcat tgtaagtagc acattgcaac
     3061 aacaatcatg cttatgacca atacagtcac taggttgtag ttttttttaa ataaaggaaa
     3121 agcagtattg tcctggtttt aaacctatga tggaattcta atgtcattat tttaatggaa
     3181 tcaatcgaaa tatgctctat agagaatata tcttttatat attgctgcag tttccttatg
     3241 ttaatccttt aacactaagg taacatgaca taatcatacc atagaaggga acacaggtta
     3301 ccatattggt ttgtaatatg ggtcttggtg ggttttgttt tatcctttaa attttgttcc
     3361 catgagtttt gtggggatgg ggattctggt tttattagct ttgtgtgtgt cctcttcccc
     3421 caaaccccct tttggtgaga acatcccctt gacagttgca gcctcttgac ctcggataac
     3481 aataagagag ctcatctcat ttttactttt gaacgttggc cttacaatca aatgtaagtt
     3541 atatatattt gtactgatga aaatttataa tctgctttaa caaaaataaa tgttcatggt
     3601 agaa
//
