LOCUS       NM_145697               1964 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens NUF2 component of NDC80 kinetochore complex (NUF2),
            transcript variant 1, mRNA.
ACCESSION   NM_145697
VERSION     NM_145697.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Bao,L., Gong,Y., Che,Y., Li,Y., Xu,T., Chen,J., Wang,S., Tan,Z.,
            Huang,P., Pan,Z. and Ge,M.
  TITLE     Maintenance of magnesium homeostasis by NUF2 promotes protein
            synthesis and anaplastic thyroid cancer progression
  JOURNAL   Cell Death Dis 15 (9), 656 (2024)
   PUBMED   39242581
  REMARK    GeneRIF: Maintenance of magnesium homeostasis by NUF2 promotes
            protein synthesis and anaplastic thyroid cancer progression.
            Erratum:[Cell Death Dis. 2024 Nov 18;15(11):842. doi:
            10.1038/s41419-024-07174-8.. PMID: 39557824]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Aimaier,R., Chung,M.H., Gu,Y., Yu,Q., Wei,C., Li,H., Guo,Z.,
            Long,M., Li,Y., Wang,W., Li,Q. and Wang,Z.
  TITLE     FOXM1 promotes neurofibromatosis type 1-associated malignant
            peripheral nerve sheath tumor progression in a NUF2-dependent
            manner
  JOURNAL   Cancer Gene Ther 30 (10), 1390-1402 (2023)
   PUBMED   37488294
  REMARK    GeneRIF: FOXM1 promotes neurofibromatosis type 1-associated
            malignant peripheral nerve sheath tumor progression in a
            NUF2-dependent manner.
REFERENCE   3  (bases 1 to 1964)
  AUTHORS   Zheng,B., Wang,S., Yuan,X., Zhang,J., Shen,Z. and Ge,C.
  TITLE     NUF2 is correlated with a poor prognosis and immune infiltration in
            clear cell renal cell carcinoma
  JOURNAL   BMC Urol 23 (1), 82 (2023)
   PUBMED   37138262
  REMARK    GeneRIF: NUF2 is correlated with a poor prognosis and immune
            infiltration in clear cell renal cell carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1964)
  AUTHORS   Shan,J., Jiang,W., Chang,J., Zhou,T., Chen,Y., Zhang,Y., Wang,J.,
            Wang,Y., Wang,Y., Xu,X., Liu,S., Shi,X., Fan,S., Chen,R., Li,C. and
            Li,X.
  TITLE     NUF2 Drives Cholangiocarcinoma Progression and Migration via
            Inhibiting Autophagic Degradation of TFR1
  JOURNAL   Int J Biol Sci 19 (5), 1336-1351 (2023)
   PUBMED   37056930
  REMARK    GeneRIF: NUF2 Drives Cholangiocarcinoma Progression and Migration
            via Inhibiting Autophagic Degradation of TFR1.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1964)
  AUTHORS   Deng,Y., Li,J., Zhang,Y., Hu,H., Wan,F., Min,H., Zhou,H., Gu,L.,
            Liao,X., Zhou,J. and Zhou,J.
  TITLE     NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell
            Indicator
  JOURNAL   Int J Mol Sci 24 (4), 4226 (2023)
   PUBMED   36835637
  REMARK    GeneRIF: NUF2 Promotes Breast Cancer Development as a New Tumor
            Stem Cell Indicator.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1964)
  AUTHORS   Tien,A.C., Lin,M.H., Su,L.J., Hong,Y.R., Cheng,T.S., Lee,Y.C.,
            Lin,W.J., Still,I.H. and Huang,C.Y.
  TITLE     Identification of the substrates and interaction proteins of aurora
            kinases from a protein-protein interaction model
  JOURNAL   Mol Cell Proteomics 3 (1), 93-104 (2004)
   PUBMED   14602875
REFERENCE   7  (bases 1 to 1964)
  AUTHORS   DeLuca,J.G., Howell,B.J., Canman,J.C., Hickey,J.M., Fang,G. and
            Salmon,E.D.
  TITLE     Nuf2 and Hec1 are required for retention of the checkpoint proteins
            Mad1 and Mad2 to kinetochores
  JOURNAL   Curr Biol 13 (23), 2103-2109 (2003)
   PUBMED   14654001
REFERENCE   8  (bases 1 to 1964)
  AUTHORS   DeLuca,J.G., Moree,B., Hickey,J.M., Kilmartin,J.V. and Salmon,E.D.
  TITLE     hNuf2 inhibition blocks stable kinetochore-microtubule attachment
            and induces mitotic cell death in HeLa cells
  JOURNAL   J Cell Biol 159 (4), 549-555 (2002)
   PUBMED   12438418
  REMARK    GeneRIF: Data highlight a specific role for hNuf2 in
            kinetochore-microtubule attachment and suggest that hNuf2 is part
            of a molecular linker between the kinetochore attachment site and
            tubulin subunits within the lattice of attached plus ends.
REFERENCE   9  (bases 1 to 1964)
  AUTHORS   Nabetani,A., Koujin,T., Tsutsumi,C., Haraguchi,T. and Hiraoka,Y.
  TITLE     A conserved protein, Nuf2, is implicated in connecting the
            centromere to the spindle during chromosome segregation: a link
            between the kinetochore function and the spindle checkpoint
  JOURNAL   Chromosoma 110 (5), 322-334 (2001)
   PUBMED   11685532
REFERENCE   10 (bases 1 to 1964)
  AUTHORS   Wigge,P.A. and Kilmartin,J.V.
  TITLE     The Ndc80p complex from Saccharomyces cerevisiae contains conserved
            centromere components and has a function in chromosome segregation
  JOURNAL   J Cell Biol 152 (2), 349-360 (2001)
   PUBMED   11266451
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK093348.1 and AL592435.2.
            
            On Nov 22, 2018 this sequence version replaced NM_145697.2.
            
            Summary: This gene encodes a protein that is highly similar to
            yeast Nuf2, a component of a conserved protein complex associated
            with the centromere. Yeast Nuf2 disappears from the centromere
            during meiotic prophase when centromeres lose their connection to
            the spindle pole body, and plays a regulatory role in chromosome
            segregation. The encoded protein is found to be associated with
            centromeres of mitotic HeLa cells, which suggests that this protein
            is a functional homolog of yeast Nuf2. Alternatively spliced
            transcript variants that encode the same protein have been
            described. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK093348.1, BG354574.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000271452.8/ ENSP00000271452.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1962              AK093348.1         1-1962
            1963-1964           AL592435.2         100243-100244
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q23.3"
     gene            1..1964
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /note="NUF2 component of NDC80 kinetochore complex"
                     /db_xref="GeneID:83540"
                     /db_xref="HGNC:HGNC:14621"
                     /db_xref="MIM:611772"
     exon            1..259
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            260..402
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     CDS             280..1674
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /note="cancer/testis antigen 106; cell division cycle
                     associated 1; hNuf2; hNuf2R; hsNuf2; cell division
                     cycle-associated protein 1; NUF2, NDC80 kinetochore
                     complex component, homolog; NDC80 kinetochore complex
                     component NUF2"
                     /codon_start=1
                     /product="kinetochore protein Nuf2"
                     /protein_id="NP_663735.2"
                     /db_xref="CCDS:CCDS1245.1"
                     /db_xref="GeneID:83540"
                     /db_xref="HGNC:HGNC:14621"
                     /db_xref="MIM:611772"
                     /translation="METLSFPRYNVAEIVIHIRNKILTGADGKNLTKNDLYPNPKPEV
                     LHMIYMRALQIVYGIRLEHFYMMPVNSEVMYPHLMEGFLPFSNLVTHLDSFLPICRVN
                     DFETADILCPKAKRTSRFLSGIINFIHFREACRETYMEFLWQYKSSADKMQQLNAAHQ
                     EALMKLERLDSVPVEEQEEFKQLSDGIQELQQSLNQDFHQKTIVLQEGNSQKKSNISE
                     KTKRLNELKLSVVSLKEIQESLKTKIVDSPEKLKNYKEKMKDTVQKLKNARQEVVEKY
                     EIYGDSVDCLPSCQLEVQLYQKKIQDLSDNREKLASILKESLNLEDQIESDESELKKL
                     KTEENSFKRLMIVKKEKLATAQFKINKKHEDVKQYKRTVIEDCNKVQEKRGAVYERVT
                     TINQEIQKIKLGIQQLKDAAEREKLKSQEIFLNLKTALEKYHDGIEKAAEDSYAKIDE
                     KTAELKRKMFKMST"
     exon            403..477
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            478..554
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            555..616
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            617..714
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            715..788
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            789..885
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            886..948
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1086
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1227
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1403
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1539
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1964
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1941..1946
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /note="hexamer: AATATA"
     polyA_site      1964
                     /gene="NUF2"
                     /gene_synonym="CDCA1; CT106; NUF2R"
                     /note="major polyA site"
ORIGIN      
        1 agtaggaggc ggcaagtttg aaaagtgatg acggttgacg tttgctgatt tttgactttg
       61 cttgtagctg ctccccgaac tcgccgtctt cctgtcggcg gccggcactg taggtgagcg
      121 cgagaggacg gaggaaggaa gcctgcagac agacgccttc tccatcccaa ggcgcgggca
      181 ggtgccggga cgctgggcct ggcggtgttt tcgtcgtgct cagcggtggg aggaggcgga
      241 agaaaccaga gcctgggaga ttaacaggaa acttccaaga tggaaacttt gtctttcccc
      301 agatataatg tagctgagat tgtgattcat attcgcaata agatcttaac aggagctgat
      361 ggtaaaaacc tcaccaagaa tgatctttat ccaaatccaa agcctgaagt cttgcacatg
      421 atctacatga gagccttaca aatagtatat ggaattcgac tggaacattt ttacatgatg
      481 ccagtgaact ctgaagtcat gtatccacat ttaatggaag gcttcttacc attcagcaat
      541 ttagttactc atctggactc atttttgcct atctgccggg tgaatgactt tgagactgct
      601 gatattctat gtccaaaagc aaaacggaca agtcggtttt taagtggcat tatcaacttt
      661 attcacttca gagaagcatg ccgtgaaacg tatatggaat ttctttggca atataaatcc
      721 tctgcggaca aaatgcaaca gttaaacgcc gcacaccagg aggcattaat gaaactggag
      781 agacttgatt ctgttccagt tgaagagcaa gaagagttca agcagctttc agatggaatt
      841 caggagctac aacaatcact aaatcaggat tttcatcaaa aaacgatagt gctgcaagag
      901 ggaaattccc aaaagaagtc aaatatttca gagaaaacca agcgtttgaa tgaactaaaa
      961 ttgtcggtgg tttctttgaa agaaatacaa gagagtttga aaacaaaaat tgtggattct
     1021 ccagagaagt taaagaatta taaagaaaaa atgaaagata cggtccagaa gcttaaaaat
     1081 gccagacaag aagtggtgga gaaatatgaa atctatggag actcagttga ctgcctgcct
     1141 tcatgtcagt tggaagtgca gttatatcaa aagaaaatac aggacctttc agataatagg
     1201 gaaaaattag ccagtatctt aaaggagagc ctgaacttgg aggaccaaat tgagagtgat
     1261 gagtcagaac tgaagaaatt gaagactgaa gaaaattcgt tcaaaagact gatgattgtg
     1321 aagaaggaaa aacttgccac agcacaattc aaaataaata agaagcatga agatgttaag
     1381 caatacaaac gcacagtaat tgaggattgc aataaagttc aagaaaaaag aggtgctgtc
     1441 tatgaacgag taaccacaat taatcaagaa atccaaaaaa ttaaacttgg aattcaacaa
     1501 ctaaaagatg ctgctgaaag ggagaaactg aagtcccagg aaatatttct aaacttgaaa
     1561 actgctttgg agaaatacca cgacggtatt gaaaaggcag cagaggactc ctatgctaag
     1621 atagatgaga agacagctga actgaagagg aagatgttca aaatgtcaac ctgattaaca
     1681 aaattacatg tctttttgta aatggcttgc catcttttaa ttttctattt agaaagaaaa
     1741 gttgaagcga atggaagtat cagaagtacc aaataatgtt ggcttcatca gtttttatac
     1801 actctcataa gtagttaata agatgaattt aatgtaggct tttattaatt tataattaaa
     1861 ataacttgtg cagctattca tgtctctact ctgccccttg ttgtaaatag tttgagtaaa
     1921 acaaaactag ttacctttga aatatatata tttttttctg ttac
//
