LOCUS       NM_024047               2820 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens nudix hydrolase 9 (NUDT9), transcript variant 1, mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_024047 NM_198039
VERSION     NM_024047.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Debar,L., Ishak,L., Moretton,A., Anoosheh,S., Morel,F.,
            Jenninger,L., Garreau-Balandier,I., Vernet,P., Hofer,A., van den
            Wildenberg,S. and Farge,G.
  TITLE     NUDT6 and NUDT9, two mitochondrial members of the NUDIX family,
            have distinct hydrolysis activities
  JOURNAL   Mitochondrion 71, 93-103 (2023)
   PUBMED   37343711
  REMARK    GeneRIF: NUDT6 and NUDT9, two mitochondrial members of the NUDIX
            family, have distinct hydrolysis activities.
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 2820)
  AUTHORS   Gattkowski,E., Rutherford,T.J., Mockl,F., Bauche,A., Sander,S.,
            Fliegert,R. and Tidow,H.
  TITLE     Analysis of ligand binding and resulting conformational changes in
            pyrophosphatase NUDT9
  JOURNAL   FEBS J 288 (23), 6769-6782 (2021)
   PUBMED   34189846
  REMARK    GeneRIF: Analysis of ligand binding and resulting conformational
            changes in pyrophosphatase NUDT9.
REFERENCE   4  (bases 1 to 2820)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2820)
  AUTHORS   Yoon,B., Yang,E.G. and Kim,S.Y.
  TITLE     The ADP-ribose reactive NUDIX hydrolase isoforms can modulate
            HIF-1alpha in cancer cells
  JOURNAL   Biochem Biophys Res Commun 504 (1), 321-327 (2018)
   PUBMED   30190133
  REMARK    GeneRIF: ADP-ribose reactive NUDT9 NUDIX hydrolase isoforms
            modulate HIF-1alpha in cancer cells
REFERENCE   6  (bases 1 to 2820)
  AUTHORS   Shen,B.W., Perraud,A.L., Scharenberg,A. and Stoddard,B.L.
  TITLE     The crystal structure and mutational analysis of human NUDT9
  JOURNAL   J Mol Biol 332 (2), 385-398 (2003)
   PUBMED   12948489
  REMARK    GeneRIF: Data report the crystal structures of NUDT9 in the
            presence and in the absence of the reaction product ribose
            5'-phosphate.
REFERENCE   7  (bases 1 to 2820)
  AUTHORS   Zhang,H.T., Yan,Z.Q., Hu,X.B., Yang,S.L. and Gong,Y.
  TITLE     Interaction of C17orf25 with ADP-ribose pyrophosphatase NUDT9
            detected via yeast two-hybrid method
  JOURNAL   Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35 (8),
            747-751 (2003)
   PUBMED   12897971
REFERENCE   8  (bases 1 to 2820)
  AUTHORS   Perraud,A.L., Shen,B., Dunn,C.A., Rippe,K., Smith,M.K.,
            Bessman,M.J., Stoddard,B.L. and Scharenberg,A.M.
  TITLE     NUDT9, a member of the Nudix hydrolase family, is an evolutionarily
            conserved mitochondrial ADP-ribose pyrophosphatase
  JOURNAL   J Biol Chem 278 (3), 1794-1801 (2003)
   PUBMED   12427752
  REMARK    GeneRIF: analysis of the human NUDT9 gene and its potential
            alternative transcripts along with detailed studies of the
            enzymatic properties and cell biological behavior of human NUDT9
            protein
REFERENCE   9  (bases 1 to 2820)
  AUTHORS   Lin,S., Gasmi,L., Xie,Y., Ying,K., Gu,S., Wang,Z., Jin,H., Chao,Y.,
            Wu,C., Zhou,Z., Tang,R., Mao,Y. and McLennan,A.G.
  TITLE     Cloning, expression and characterisation of a human Nudix hydrolase
            specific for adenosine 5'-diphosphoribose (ADP-ribose)
  JOURNAL   Biochim Biophys Acta 1594 (1), 127-135 (2002)
   PUBMED   11825615
  REMARK    GeneRIF: Hydrolyses ADP-ribose to AMP and ribose-5-phosphate
REFERENCE   10 (bases 1 to 2820)
  AUTHORS   Perraud,A.L., Fleig,A., Dunn,C.A., Bagley,L.A., Launay,P.,
            Schmitz,C., Stokes,A.J., Zhu,Q., Bessman,M.J., Penner,R.,
            Kinet,J.P. and Scharenberg,A.M.
  TITLE     ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed
            by Nudix motif homology
  JOURNAL   Nature 411 (6837), 595-599 (2001)
   PUBMED   11385575
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC112250.4, BP277630.1,
            AK027295.1 and AK074845.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_024047.4.
            
            Summary: The protein encoded by this gene belongs to the Nudix
            hydrolase family. Nudix boxes are found in a family of diverse
            enzymes that catalyze the hydrolysis of nucleoside diphosphate
            derivatives. This enzyme is an ADP-ribose pyrophosphatase that
            catalyzes the hydrolysis of ADP-ribose to AMP and ribose-5-P. It
            requires divalent metal ions and an intact Nudix motif for
            enzymatic activity. Alternatively spliced transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (1, also known as NUDT9 alpha)
            represents the predominant transcript, and encodes the longest
            isoform (a). This isoform is localized in the mitochondria
            (PMID:12427752).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.261349.1,
                                           SRR1660803.62581.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 12427752
            MANE Ensembl match                 :: ENST00000302174.9/
                                                  ENSP00000303575.4
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AC112250.4         14381-14383
            4-13                BP277630.1         1-10
            14-1169             AK027295.1         1-1156
            1170-1755           AK074845.1         809-1394
            1756-1756           AC112250.4         50199-50199
            1757-2819           AK074845.1         1396-2458
            2820-2820           AC112250.4         51263-51263
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q22.1"
     gene            1..2820
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /note="nudix hydrolase 9"
                     /db_xref="GeneID:53343"
                     /db_xref="HGNC:HGNC:8056"
                     /db_xref="MIM:606022"
     exon            1..440
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /inference="alignment:Splign:2.1.0"
     CDS             334..1386
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /EC_number="3.6.1.13"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; nucleoside diphosphate linked moiety X-type
                     motif 9; ADP-ribose diphosphatase; adenosine
                     diphosphoribose pyrophosphatase; ADP-ribose
                     phosphohydrolase; ADP-ribose pyrosphosphatase NUDT9;
                     ADP-ribose pyrophosphatase, mitochondrial; ADPR-PPase;
                     nudix (nucleoside diphosphate linked moiety X)-type motif
                     9"
                     /codon_start=1
                     /product="ADP-ribose pyrophosphatase, mitochondrial
                     isoform a precursor"
                     /protein_id="NP_076952.1"
                     /db_xref="CCDS:CCDS3620.1"
                     /db_xref="GeneID:53343"
                     /db_xref="HGNC:HGNC:8056"
                     /db_xref="MIM:606022"
                     /translation="MAGRLLGKALAAVSLSLALASVTIRSSRCRGIQAFRNSFSSSWF
                     HLNTNVMSGSNGSKENSHNKARTSPYPGSKVERSQVPNEKVGWLVEWQDYKPVEYTAV
                     SVLAGPRWADPQISESNFSPKFNEKDGHVERKSKNGLYEIENGRPRNPAGRTGLVGRG
                     LLGRWGPNHAADPIITRWKRDSSGNKIMHPVSGKHILQFVAIKRKDCGEWAIPGGMVD
                     PGEKISATLKREFGEEALNSLQKTSAEKREIEEKLHKLFSQDHLVIYKGYVDDPRNTD
                     NAWMETEAVNYHDETGEIMDNLMLEAGDDAGKVKWVDINDKLKLYASHSQFIKLVAEK
                     RDAHWSEDSEADCHAL"
     exon            441..680
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /inference="alignment:Splign:2.1.0"
     exon            681..776
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /inference="alignment:Splign:2.1.0"
     exon            777..863
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /inference="alignment:Splign:2.1.0"
     exon            864..975
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1122
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1207
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..2820
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1684..1689
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /note="hexamer: AATAAA"
     regulatory      1690..1695
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /note="hexamer: GATAAA"
     polyA_site      1709
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
     polyA_site      1712
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /note="major polyA site"
     regulatory      2761..2766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /note="hexamer: AACAAA"
     regulatory      2798..2803
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
                     /note="hexamer: AATAAA"
     polyA_site      2799
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
     polyA_site      2820
                     /gene="NUDT9"
                     /gene_synonym="NUDT10"
ORIGIN      
        1 gcggcgcggc cgtaaagcgc cattacgcag agagaaagtt acgaggttcg tggccgcggt
       61 ttccccaggc agctggcgct ggaggcttcg gcgtcacgtg ctggtctgga tttttctcga
      121 tgcactgggg aaagcggtgg actcttatcg tgggagggct cttgatctgt gatttataga
      181 taggcacagc tactcccgtt cgggaaccca acggcagaca ggtcctagtg cccatcagat
      241 acccgcggcc gggactcgga gctgtggggt gtggggaggc ggaggcacca actaagagcg
      301 acctagcatc gcaaagccgc cctcggggcg ctcatggcgg gacgcctcct gggaaaggct
      361 ttagccgcgg tgtctctctc tctggccttg gcctctgtga ctatcaggtc ctcgcgctgc
      421 cgcggcatcc aggcgttcag aaactcgttt tcatcttctt ggtttcatct taataccaac
      481 gtcatgtctg gttctaatgg ttccaaagaa aattctcaca ataaggctcg gacgtctcct
      541 tacccaggtt caaaagttga acgaagccag gttcctaatg agaaagtggg ctggcttgtt
      601 gagtggcaag actataagcc tgtggaatac actgcagtct ctgtcttggc tggacccagg
      661 tgggcagatc ctcagatcag tgaaagtaat ttttctccca agtttaacga aaaggatggg
      721 catgttgaga gaaagagcaa gaatggcctg tatgagattg aaaatggaag accgagaaat
      781 cctgcaggac ggactggact ggtgggccgg gggcttttgg ggcgatgggg cccaaatcac
      841 gctgcagatc ccattataac cagatggaaa agggatagca gtggaaataa aatcatgcat
      901 cctgtttctg ggaagcatat cttacaattt gttgcaataa aaaggaaaga ctgtggagaa
      961 tgggcaatcc caggggggat ggtggatcca ggagagaaga ttagtgccac actgaaaaga
     1021 gaatttggtg aggaagctct caactcctta cagaaaacca gtgctgagaa gagagaaata
     1081 gaggaaaagt tgcacaaact cttcagccaa gaccacctag tgatatataa gggatatgtt
     1141 gatgatcctc gaaacactga taatgcatgg atggagacag aagctgtgaa ctaccatgac
     1201 gaaacaggtg agataatgga taatcttatg ctagaagctg gagatgatgc tggaaaagtg
     1261 aaatgggtgg acatcaatga taaactgaag ctttatgcca gtcactctca attcatcaaa
     1321 cttgtggctg agaaacgaga tgcacactgg agcgaggact ctgaagctga ctgccatgcg
     1381 ttgtagctga tggtctccgt gtaagccaaa ggcccacaga ggagcatata ctgaaaagaa
     1441 ggcagtatca cagaatttat actataaaaa gggcagggta ggccacttgg cctatttact
     1501 ttcaaaacaa tttgcattta gagtgtttcg catcagaata acatgagtaa gatgaactgg
     1561 aacacaaaat tttcagctct ttggtcaaaa ggaatataag taatcatatt ttgtatgtat
     1621 tcgatttaag catggcttaa attaaattta aacaactaat gctctttgaa gaatcataat
     1681 cagaataaag ataaattctt gatcagctat aacttctgtt tcatctagtt cttgtttgtc
     1741 aatgccttcc ctcccgctcc ccatcttctg aggcctgaag gatgttcctg ccacatcaaa
     1801 ctcctttagg cagagtaccg ccactggatg tatagtttaa tgtttcatct gatttttctt
     1861 taaattattt ccttcacatt ttgcttcaca tgatcttgtt gaagctaatt ttgaaaatgg
     1921 aaactatgtt ttcttgactt tagaagcatt agttttaaga ggaaatttca gccactagtc
     1981 aatagagatc aaaatcagtg aacaagagct gatactctca ttttcaaaaa ttctgaatta
     2041 aaaaaagtcc aggaagttag taatttaaat cagtatgttc atttcactct agtgaaacta
     2101 ctatatgttg taaattagtt aaaaatggat gttcagaaaa gaattgaaat caggacaaaa
     2161 aattacagat gctagtgagg ttgtagagaa aaaggaatgc ttatacgctg ttagtgggag
     2221 tgtaaattgg tccaaccatt gtggaagaga gtgtagagag tcctcaaata tttaaagata
     2281 tttgacccag taatcccatt actaggtata tacccgaagg aatataaatt gttctattat
     2341 aaacacatac tcacgtgtat gttcattgct gcactattca caatagcgaa gacatgaaat
     2401 caacctaaat gttcatcagt gatagactgg ataaagacaa tgtggtatat gtacaccatg
     2461 gaatactatg cagccgtaaa aaagaataag atcatgtcct ctgaaaagac atgggtggag
     2521 ctggaggcca ttatccttag caaactaatg caagaacaga aaaccaaaca ctgcatgttc
     2581 tcttataagt gagagctaaa tggtgagaac acatggacat gggaacaaca cattctgggg
     2641 tctattggag ggtgggagga gggagaggat caggaaaaat aactaatgag tactaggctt
     2701 aataccttgg tgatgaaatt atctgtacaa caaaccctca tgacacaagt ttacctatgt
     2761 aacaaacctg cacatgtacc cctgtactta acaacaaaat aaacacaaag gaaaatgtta
//
