LOCUS       NM_014142               3195 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens nudix hydrolase 5 (NUDT5), transcript variant 1, mRNA.
ACCESSION   NM_014142
VERSION     NM_014142.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Qian,J., Ma,Y., Tahaney,W.M., Moyer,C.L., Lanier,A., Hill,J.,
            Coleman,D., Koupaei,N., Hilsenbeck,S.G., Savage,M.I., Page,B.D.G.,
            Mazumdar,A. and Brown,P.H.
  TITLE     The novel phosphatase NUDT5 is a critical regulator of
            triple-negative breast cancer growth
  JOURNAL   Breast Cancer Res 26 (1), 23 (2024)
   PUBMED   38317231
  REMARK    GeneRIF: The novel phosphatase NUDT5 is a critical regulator of
            triple-negative breast cancer growth.
            Erratum:[Breast Cancer Res. 2024 Mar 26;26(1):53. doi:
            10.1186/s13058-024-01814-9.. PMID: 38532428]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Ding,H., Wu,C., Sun,W., Zhan,Q., Huang,Y., Liao,N., Jiang,Z.,
            Wang,K. and Li,Y.
  TITLE     NUDT5-Determines the fate of head and neck squamous cell carcinoma
            cells under endoplasmic reticulum stress by catalyzing nuclear ATP
            production to promote DNA repair
  JOURNAL   Oral Oncol 141, 106397 (2023)
   PUBMED   37156197
  REMARK    GeneRIF: NUDT5-Determines the fate of head and neck squamous cell
            carcinoma cells under endoplasmic reticulum stress by catalyzing
            nuclear ATP production to promote DNA repair.
REFERENCE   3  (bases 1 to 3195)
  AUTHORS   Tan,D. and Zhang,Y.
  TITLE     Silencing of Nudix type 5 represses proliferation and invasion and
            enhances chemosensitivity of gastric carcinoma cells by affecting
            the AKT/GSK-3beta/beta-catenin pathway
  JOURNAL   Toxicol Appl Pharmacol 441, 115968 (2022)
   PUBMED   35247377
  REMARK    GeneRIF: Silencing of Nudix type 5 represses proliferation and
            invasion and enhances chemosensitivity of gastric carcinoma cells
            by affecting the AKT/GSK-3beta/beta-catenin pathway.
REFERENCE   4  (bases 1 to 3195)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3195)
  AUTHORS   Zhang,H., Zhang,L.Q., Yang,C.C., Li,J., Tian,X.Y., Li,D.N., Cui,J.
            and Cai,J.P.
  TITLE     The high expression of NUDT5 indicates poor prognosis of breast
            cancer by modulating AKT / Cyclin D signaling
  JOURNAL   PLoS One 16 (2), e0245876 (2021)
   PUBMED   33571243
  REMARK    GeneRIF: The high expression of NUDT5 indicates poor prognosis of
            breast cancer by modulating AKT / Cyclin D signaling.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3195)
  AUTHORS   Ishibashi,T., Hayakawa,H., Ito,R., Miyazawa,M., Yamagata,Y. and
            Sekiguchi,M.
  TITLE     Mammalian enzymes for preventing transcriptional errors caused by
            oxidative damage
  JOURNAL   Nucleic Acids Res 33 (12), 3779-3784 (2005)
   PUBMED   16002790
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3195)
  AUTHORS   Ishibashi,T., Hayakawa,H. and Sekiguchi,M.
  TITLE     A novel mechanism for preventing mutations caused by oxidation of
            guanine nucleotides
  JOURNAL   EMBO Rep 4 (5), 479-483 (2003)
   PUBMED   12717453
  REMARK    GeneRIF: degrades 8-oxo-dGDP to 8-oxo-dGMP, an unusable form for
            DNA synthesis, and promotes the cleavage of 8-oxo-dGTP by MTH1 to
            yield 8-oxo-dGMP also
REFERENCE   8  (bases 1 to 3195)
  AUTHORS   Yang,H., Slupska,M.M., Wei,Y.F., Tai,J.H., Luther,W.M., Xia,Y.R.,
            Shih,D.M., Chiang,J.H., Baikalov,C., Fitz-Gibbon,S., Phan,I.T.,
            Conrad,A. and Miller,J.H.
  TITLE     Cloning and characterization of a new member of the Nudix
            hydrolases from human and mouse
  JOURNAL   J Biol Chem 275 (12), 8844-8853 (2000)
   PUBMED   10722730
REFERENCE   9  (bases 1 to 3195)
  AUTHORS   Gasmi,L., Cartwright,J.L. and McLennan,A.G.
  TITLE     Cloning, expression and characterization of YSA1H, a human
            adenosine 5'-diphosphosugar pyrophosphatase possessing a MutT motif
  JOURNAL   Biochem J 344 Pt 2 (Pt 2), 331-337 (1999)
   PUBMED   10567213
REFERENCE   10 (bases 1 to 3195)
  AUTHORS   McLennan,A.G.
  TITLE     The MutT motif family of nucleotide phosphohydrolases in man and
            human pathogens (review)
  JOURNAL   Int J Mol Med 4 (1), 79-89 (1999)
   PUBMED   10373642
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC000025.2, AF155832.1, AC073160.8 and CA944803.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014142.3.
            
            Summary: This gene belongs to the Nudix (nucleoside diphosphate
            linked moiety X) hydrolase superfamily. The encoded enzyme
            catalyzes the hydrolysis of modified nucleoside diphosphates,
            including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and
            8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell
            because it can modify some amino acid residues, resulting in the
            inhibition of ATP-activated potassium channels. 8-oxoGua is an
            oxidized form of guanine that can potentially alter genetic
            information by pairing with adenine and cytosine in RNA. Presence
            of 8-oxoGua in RNA results in formation of abnormal proteins due to
            translational errors. [provided by RefSeq, Aug 2013].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.15842.1,
                                           SRR1660805.30308.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000491614.6/ ENSP00000419628.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-122               BC000025.2         165-286
            123-771             AF155832.1         122-770
            772-951             BC000025.2         936-1115
            952-2882            AC073160.8         162525-164455       c
            2883-3195           CA944803.1         18-330              c
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p14"
     gene            1..3195
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /note="nudix hydrolase 5"
                     /db_xref="GeneID:11164"
                     /db_xref="HGNC:HGNC:8052"
                     /db_xref="MIM:609230"
     exon            1..122
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /inference="alignment:Splign:2.1.0"
     exon            123..226
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             164..823
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /EC_number="3.6.1.13"
                     /EC_number="3.6.1.58"
                     /EC_number="2.7.7.96"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ADP-sugar pyrophosphatase; 8-oxo-dGDP phosphatase; nudix
                     (nucleoside diphosphate linked moiety X)-type motif 5;
                     nuclear ATP-synthesis protein NUDIX5"
                     /codon_start=1
                     /product="ADP-sugar pyrophosphatase isoform 1"
                     /protein_id="NP_054861.2"
                     /db_xref="CCDS:CCDS7089.1"
                     /db_xref="GeneID:11164"
                     /db_xref="HGNC:HGNC:8052"
                     /db_xref="MIM:609230"
                     /translation="MESQEPTESSQNGKQYIISEELISEGKWVKLEKTTYMDPTGKTR
                     TWESVKRTTRKEQTADGVAVIPVLQRTLHYECIVLVKQFRPPMGGYCIEFPAGLIDDG
                     ETPEAAALRELEEETGYKGDIAECSPAVCMDPGLSNCTIHIVTVTINGDDAENARPKP
                     KPGDGEFVEVISLPKNDLLQRLDALVAEEHLTVDARVYSYALALKHANAKPFEVPFLK
                     F"
     exon            227..294
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /inference="alignment:Splign:2.1.0"
     exon            295..344
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /inference="alignment:Splign:2.1.0"
     exon            345..452
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /inference="alignment:Splign:2.1.0"
     exon            453..548
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /inference="alignment:Splign:2.1.0"
     exon            549..650
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /inference="alignment:Splign:2.1.0"
     exon            651..659
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /inference="alignment:Splign:2.1.0"
     exon            660..713
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /inference="alignment:Splign:2.1.0"
     exon            714..3195
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      933..938
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /note="hexamer: AATAAA"
     polyA_site      951
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /note="major polyA site"
     regulatory      1029..1034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /note="hexamer: AACAAA"
     polyA_site      1048
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
     regulatory      3170..3175
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
                     /note="hexamer: AATAAA"
     polyA_site      3195
                     /gene="NUDT5"
                     /gene_synonym="hNUDT5; YSA1; YSA1H; YSAH1"
ORIGIN      
        1 gcccatcctt ttagcaccgc gagaggcgcc ggtgtttcga gccgtggcac cggcatcggc
       61 tgacactgct gcctccagct agttatttcg tcctcttccg ttcttcaccc ctacaccttg
      121 gaggtgaact tctcacctga gggctgtaaa gactcgtttg aaaatggaga gccaagaacc
      181 aacggaatct tctcagaatg gcaaacagta tatcatttca gaggagttaa tttcagaagg
      241 aaaatgggtc aagcttgaaa aaacaacgta catggatcct actggtaaaa ctagaacttg
      301 ggaatcagtg aaacgtacaa ccaggaaaga gcagactgcg gatggtgtcg cggtcatccc
      361 cgtgctgcag agaacacttc actatgagtg tatcgttctg gtgaaacagt tccgaccacc
      421 aatggggggc tactgcatag agttccctgc aggtctcata gatgatggtg aaaccccaga
      481 agcagctgct ctccgggagc ttgaagaaga aactggctac aaaggggaca ttgccgaatg
      541 ttctccagcg gtctgtatgg acccaggctt gtcaaactgt actatacaca tcgtgacagt
      601 caccattaac ggagatgatg ccgaaaacgc aaggccgaag ccaaagccag gggatggaga
      661 gtttgtggaa gtcatttctt tacccaagaa tgacctgctg cagagacttg atgctctggt
      721 agctgaagaa catctcacag tggacgccag ggtctattcc tacgctctag cactgaaaca
      781 tgcaaatgca aagccatttg aagtgccctt cttgaaattt taagcccaaa tatgacactg
      841 gccatttttg taaacgagac caccaggcct tcttcactaa gactttgtat tcaacttagt
      901 ttaatgtaga tttgccatta gctttttcgt aaaataaaag cacagaacag atgtggtggt
      961 ggtatggaat tgtaattaca ggtaggttgt gaccttcctt taaatttgtt ataactccag
     1021 ctaaaattaa caaagaatat aaatgcaagt atgtttactc caattttttt aaagctcaac
     1081 agcagttaac tacagctcag ttacttttct agtccagtct ggtagacagg ggtatttgta
     1141 tagagaaata gacctgagtt ctcaattagg tcatttccac tctcaacccc caatataaaa
     1201 ttctaagctg ggtatgcaaa taaccatgta ctccgttagt gtttaaaaca gcgttaaaaa
     1261 gaaaaaaaag gctttttggc ctcttctaat tgaattttta ctatgcaaaa tttatctagg
     1321 gtagttgaat ccaactaggg tagggttcga tagaatcctc tactagtttt cagttcatct
     1381 atttactgag gctgaaccag cttttaagta aggaaaacgg ttgactccat tcactacacc
     1441 aaggctagga gactaatgaa aattcaggtc catcccgcaa gcatctgcac gtgcttgagg
     1501 atctcttttc ctattcttac ctcaaagagt acttgaagtt tctgaaaaca aatctgtaag
     1561 actgacatgt ttggggcagt aatatcttga tgttaaacca tgaacagaac cagcaagatc
     1621 agccagtacc tgaaacccaa tacagagtag ttcacagcaa gaagtacaga ttgatctggt
     1681 tcccatgcct gaaaccctgt catctagcag ttctaccagt gttcctgggc cattttctta
     1741 gcttcttgag tgagtttagc tctttttgtg ttgactttta gggcctccag cagctccatg
     1801 attttccagg actttccagt ctggccccca cggaattctc aggatgattc tcatccagcc
     1861 ctaagtcatg tttctagcct ggctccagcg ggtaagccag gccctgagaa ccatatgaaa
     1921 gggctctcca gataaaatca gagtgctaat gccagaatgc tgcagtagcc tccgtttgtc
     1981 cacaggctca ctgtctgagc ccaggatatt tttatgagtg aaactgtaag atctttacac
     2041 tggacttcct aaagataaaa caattatgga aatacagaac tttacagatg aagctgtcta
     2101 gccctataca tgtgaaaatt aagatccaac tacttcctgt aatggtagcc ttcccctatg
     2161 cctctaaccc ttttttaaat cctgatttca atcacaggca ttgtgattct ccaaagctct
     2221 aactcttcct taggtctgaa agccacatgc tatcaatcat tgaaatcaag agagaaggca
     2281 tccgtagacg ggcgcagccc agtctttcca catagcccag ttcctcccca gccactcccc
     2341 atgggaatga cctctgcagc tgacctaagc ttatcctctc cttcttccta agagtgcaac
     2401 ttagaagtca gagccaaatc ctagaatgta tccctaaatg ccatttctat tttttataaa
     2461 catgaaaagg ttgtgctttg gaaattcagt ctgtttgaga actggcagtc tggctggcac
     2521 tgtttaaaaa tgtttatttg ccctgcattt aaaaactgaa ggtttcctat gtattaaaag
     2581 ccatatttcc accttcttaa gggaaaaaaa aatcggacgt cataacactg ggtggatgtt
     2641 tttatggtaa cagtggtctg aggcccttgg cggatgctca ccgagccact gtcactatcc
     2701 tgcctgcctt tatctgcctt gccctgcgat tatttggctt tgtaagcata agctacgtaa
     2761 gacataccac acctaagaaa ctaaacagca atgaaaaccc atacttctga aaagggtgtc
     2821 ttttaaaaat taagtttgat cttttgggaa ataaagcaca tagttttcaa aatgatttac
     2881 caagatctat gctctttcaa atggagactg ggatattgtt gttttgagac cactgttttc
     2941 ctgtttcctc ccaaatgtta gtcacatata tgattaccct taagttggtc actatctatc
     3001 tatctgtcaa taatttagga ttaaagtttg aagtgtaatt acatttgact tttacaggca
     3061 tctcagggtg gacttcttat gaatacactg aacctataca aatgatctta gaaaacgttt
     3121 aatatcagat ttctttattt tttccaacac aactgaatca gttttcttaa ataaaagttt
     3181 attgtctact gttta
//
