LOCUS       NM_005013               2300 bp    mRNA    linear   PRI 28-AUG-2024
DEFINITION  Homo sapiens nucleobindin 2 (NUCB2), transcript variant 1, mRNA.
ACCESSION   NM_005013
VERSION     NM_005013.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ji,R., Wang,Y., Pan,D., Han,J., Wang,Y., Zheng,S., Zhao,W., Li,X.,
            Han,C. and Zhang,L.
  TITLE     NUCB2 inhibition antagonizes osteosarcoma progression and promotes
            anti-tumor immunity through inactivating NUCKS1/CXCL8 axis
  JOURNAL   Cancer Lett 591, 216893 (2024)
   PUBMED   38636892
  REMARK    GeneRIF: NUCB2 inhibition antagonizes osteosarcoma progression and
            promotes anti-tumor immunity through inactivating NUCKS1/CXCL8
            axis.
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Ishibashi,Y., Itoh,T., Oguri,Y., Hashimura,M., Yokoi,A.,
            Matsumoto,T., Harada,Y., Fukagawa,N., Hayashi,M., Ono,M., Kusano,C.
            and Saegusa,M.
  TITLE     Nucleobindin 2 inhibits senescence in gastric carcinoma
  JOURNAL   Sci Rep 14 (1), 11261 (2024)
   PUBMED   38760405
  REMARK    GeneRIF: Nucleobindin 2 inhibits senescence in gastric carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2300)
  AUTHORS   Lin,I.C., Chang,C.H., Chong,Y.B., Kuo,S.H., Cheng,Y.W., Lieu,A.S.,
            Tseng,T.T., Lin,C.J., Tsai,H.P. and Kwan,A.L.
  TITLE     Role of Nucleobindin-2 in the Clinical Pathogenesis and Treatment
            Resistance of Glioblastoma
  JOURNAL   Cells 12 (19), 2420 (2023)
   PUBMED   37830634
  REMARK    GeneRIF: Role of Nucleobindin-2 in the Clinical Pathogenesis and
            Treatment Resistance of Glioblastoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2300)
  AUTHORS   Sun,J., Gao,N., Wu,Q., Li,Y., Zhang,L., Jiang,Z., Wang,Z. and
            Liu,J.
  TITLE     High plasma nesfatin-1 level in Chinese adolescents with depression
  JOURNAL   Sci Rep 13 (1), 15288 (2023)
   PUBMED   37714885
  REMARK    GeneRIF: High plasma nesfatin-1 level in Chinese adolescents with
            depression.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2300)
  AUTHORS   Schaper,S.J., Wolk,E., Hofmann,T., Friedrich,T., Romer,M., de
            Punder,K., Rose,M. and Stengel,A.
  TITLE     NUCB2/nesfatin-1 in the acute stress response of obese women with
            high and low anxiety
  JOURNAL   Psychoneuroendocrinology 155, 106325 (2023)
   PUBMED   37385089
  REMARK    GeneRIF: NUCB2/nesfatin-1 in the acute stress response of obese
            women with high and low anxiety.
REFERENCE   6  (bases 1 to 2300)
  AUTHORS   Nesselhut,J., Jurgan,U., Onken,E., Gotz,H., Barnikol,H.U.,
            Hirschfeld,G., Barnikol-Watanabe,S. and Hilschmann,N.
  TITLE     Golgi retention of human protein NEFA is mediated by its N-terminal
            Leu/Ile-rich region
  JOURNAL   FEBS Lett 509 (3), 469-475 (2001)
   PUBMED   11749975
REFERENCE   7  (bases 1 to 2300)
  AUTHORS   Taniguchi,N., Taniura,H., Niinobe,M., Takayama,C.,
            Tominaga-Yoshino,K., Ogura,A. and Yoshikawa,K.
  TITLE     The postmitotic growth suppressor necdin interacts with a
            calcium-binding protein (NEFA) in neuronal cytoplasm
  JOURNAL   J Biol Chem 275 (41), 31674-31681 (2000)
   PUBMED   10915798
REFERENCE   8  (bases 1 to 2300)
  AUTHORS   Kroll,K.A., Otte,S., Hirschfeld,G., Barnikol-Watanabe,S., Gotz,H.,
            Sternbach,H., Kratzin,H.D., Barnikol,H.U. and Hilschmann,N.
  TITLE     Heterologous overexpression of human NEFA and studies on the two
            EF-hand calcium-binding sites
  JOURNAL   Biochem Biophys Res Commun 260 (1), 1-8 (1999)
   PUBMED   10381334
REFERENCE   9  (bases 1 to 2300)
  AUTHORS   DeAngelis,M.M., Doucet,J.P., Drury,S., Sherry,S.T., Robichaux,M.B.,
            Den,Z., Pelias,M.Z., Ditta,G.M., Keats,B.J., Deininger,P.L. and
            Batzer,M.A.
  TITLE     Assembly of a high-resolution map of the Acadian Usher syndrome
            region and localization of the nuclear EF-hand acidic gene
  JOURNAL   Biochim Biophys Acta 1407 (1), 84-91 (1998)
   PUBMED   9639681
REFERENCE   10 (bases 1 to 2300)
  AUTHORS   Barnikol-Watanabe,S., Gross,N.A., Gotz,H., Henkel,T., Karabinos,A.,
            Kratzin,H., Barnikol,H.U. and Hilschmann,N.
  TITLE     Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper
            protein. Molecular cloning and sequence analysis of the cDNA,
            isolation and characterization of the protein
  JOURNAL   Biol Chem Hoppe Seyler 375 (8), 497-512 (1994)
   PUBMED   7811391
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC107956.8 and KF455328.1.
            
            On May 17, 2019 this sequence version replaced NM_005013.3.
            
            Summary: This gene encodes a protein with a suggested role in
            calcium level maintenance, eating regulation in the hypothalamus,
            and release of tumor necrosis factor from vascular endothelial
            cells. This protein binds calcium and has EF-folding domains.
            [provided by RefSeq, Oct 2011].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.129055.1,
                                           SRR1803612.188201.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000529010.6/ ENSP00000436455.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-90                AC107956.8         106616-106705
            91-245              AC107956.8         112666-112820
            246-389             AC107956.8         125201-125344
            390-497             AC107956.8         125981-126088
            498-624             AC107956.8         131621-131747
            625-728             AC107956.8         139449-139552
            729-914             AC107956.8         140702-140887
            915-1005            AC107956.8         141070-141160
            1006-1053           AC107956.8         141749-141796
            1054-1064           KF455328.1         1-11
            1065-1157           KF455328.1         109-201
            1158-1247           AC107956.8         145263-145352
            1248-1418           AC107956.8         160004-160174
            1419-1500           AC107956.8         160779-160860
            1501-2300           AC107956.8         161289-162088
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.1"
     gene            1..2300
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /note="nucleobindin 2"
                     /db_xref="GeneID:4925"
                     /db_xref="HGNC:HGNC:8044"
                     /db_xref="MIM:608020"
     exon            1..90
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            91..245
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     CDS             246..1508
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; nucleobinding 2; DNA-binding protein NEFA;
                     gastric cancer antigen Zg4; prepronesfatin; epididymis
                     secretory protein Li 109; nesfatin 1; novel DNA
                     binding/EF-hand/leucine zipper protein"
                     /codon_start=1
                     /product="nucleobindin-2 isoform 1 preproprotein"
                     /protein_id="NP_005004.1"
                     /db_xref="CCDS:CCDS41623.1"
                     /db_xref="GeneID:4925"
                     /db_xref="HGNC:HGNC:8044"
                     /db_xref="MIM:608020"
                     /translation="MRWRTILLQYCFLLITCLLTALEAVPIDIDKTKVQNIHPVESAK
                     IEPPDTGLYYDEYLKQVIDVLETDKHFREKLQKADIEEIKSGRLSKELDLVSHHVRTK
                     LDELKRQEVGRLRMLIKAKLDSLQDIGMDHQALLKQFDHLNHLNPDKFESTDLDMLIK
                     AATSDLEHYDKTRHEEFKKYEMMKEHERREYLKTLNEEKRKEEESKFEEMKKKHENHP
                     KVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNSDGFLDEQELEALFTKELEK
                     VYDPKNEEDDMVEMEEERLRMREHVMNEVDTNKDRLVTLEEFLKATEKKEFLEPDSWE
                     TLDQQQFFTEEELKEYENIIALQENELKKKADELQKQKEELQRQHDQLEAQKLEYHQV
                     IQQMEQKKLQQGIPPSGPAGELKFEPHI"
     exon            246..389
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            390..497
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            498..624
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            625..728
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            729..914
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1005
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1064
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1157
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1247
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1418
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1500
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..2300
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1594..1599
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /note="hexamer: AATAAA"
     polyA_site      1612
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /note="major polyA site"
     regulatory      1620..1625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /note="hexamer: AATAAA"
     polyA_site      1648
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
     regulatory      1670..1675
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /note="hexamer: ATTAAA"
     polyA_site      1710
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
     regulatory      1929..1934
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
                     /note="hexamer: AATAAA"
     polyA_site      1954
                     /gene="NUCB2"
                     /gene_synonym="HEL-S-109; NEFA"
ORIGIN      
        1 agagcggagc ggtgggccgg gggctggagg acaggtttgt gcgctggacg caagcaccag
       61 gcgcagcctc gctcgccgag acccggccag aacgtgttac gagtcagttt ttagtgaaaa
      121 aacattgagc taggagccaa gacccatctc ttcactattt tggtattgtg caagtcatct
      181 tacctctctg gatctcagtt gtctcatctg taaaaaggag ataaaaatta tttacctgcc
      241 tgaacatgag gtggaggacc atcctgctac agtattgctt tctcttgatt acatgtttac
      301 ttactgctct tgaagctgtg cctattgaca tagacaagac aaaagtacaa aatattcacc
      361 ctgtggaaag tgcgaagata gaaccaccag atactggact ttattatgat gaatatctca
      421 agcaagtgat tgatgtgctg gaaacagata aacacttcag agaaaagctc cagaaagcag
      481 acatagagga aataaagagt gggaggctaa gcaaagaact ggatttagta agtcaccatg
      541 tgaggacaaa acttgatgaa ctgaaaaggc aagaagtagg aaggttaaga atgttaatta
      601 aagctaagtt ggattccctt caagatatag gcatggacca ccaagctctt ctaaaacaat
      661 ttgatcacct aaaccacctg aatcctgaca agtttgaatc cacagattta gatatgctaa
      721 tcaaagcggc aacaagtgat ctggaacact atgacaagac tcgtcatgaa gaatttaaaa
      781 aatatgaaat gatgaaggaa catgaaagga gagaatattt aaaaacattg aatgaagaaa
      841 agagaaaaga agaagagtct aaatttgaag aaatgaagaa aaagcatgaa aatcacccta
      901 aagttaatca cccaggaagc aaagatcaac taaaagaggt atgggaagag actgatggat
      961 tggatcctaa tgactttgac cccaagacat ttttcaaatt acatgatgtc aatagtgatg
     1021 gattcctgga tgaacaagaa ttagaagccc tatttactaa agagttggag aaagtatatg
     1081 accctaaaaa tgaagaggat gatatggtag aaatggaaga agaaaggctt agaatgaggg
     1141 aacatgtaat gaatgaggtt gatactaaca aagacagatt ggtgactctg gaggagtttt
     1201 tgaaagccac agaaaaaaaa gaattcttgg agccagatag ctgggagaca ttagatcagc
     1261 aacagttctt cacagaggaa gaactaaaag aatatgaaaa tattattgct ttacaagaaa
     1321 atgaacttaa gaagaaggca gatgagcttc agaaacaaaa agaagagcta caacgtcagc
     1381 atgatcaact ggaggctcag aagctggaat atcatcaggt catacagcag atggaacaaa
     1441 aaaaattaca acaaggaatt cctccatcag ggccagctgg agaattgaag tttgagccac
     1501 acatttaaag tctgaagtcc accagaactt ggaagaaagc tgttaactca acatctattt
     1561 catcttttta gctcccttcc tttttctctg ctcaataaat attttaaaag catatttgaa
     1621 ataaagggag atacttttta aatgaaaaca ctttttttgg gacacagata ttaaaggatt
     1681 gaagtttatc agaaccagga agaaaacaaa ctcactgtct gctctctgct ctcacattca
     1741 cacggctctt ttatttattt ttttgttctc ctttaatgat ttaattaagt ggctttatgc
     1801 cataatttag tgaaactatt aggaactatt taagtgagaa aactctgcct cttgctttta
     1861 aattagattg ctctcactta ctcgtaaaca taggtattct tttatgggtg cttatcattc
     1921 cttctttcaa taaatgtctg tttgatatta acaattctgg aaaggccaca gtatttccct
     1981 gtgtttcctg gtaacgtttt tctagttttg gcaacctcaa ctgctagaaa ttcttcacct
     2041 gaatcacttt tgctaccact tcaggtcatt tttcattctt ttttattttg ctctatactt
     2101 tatcatttaa gattaggtta tgttacatat aacagaaaaa acaaagataa cagtggttta
     2161 aactaaatag gagtttttct tcttacataa gtctagaagt aggtggtgtc caggttccta
     2221 tctttctgct ctgctatcct cagtgcatga tttttatcct cagattacct cactctcact
     2281 gttcaagttt gctcctggag
//
