LOCUS       NM_012225               1349 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens NUBP iron-sulfur cluster assembly factor 2, cytosolic
            (NUBP2), transcript variant 1, mRNA.
ACCESSION   NM_012225
VERSION     NM_012225.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1349)
  AUTHORS   Shin,S.Y., Fauman,E.B., Petersen,A.K., Krumsiek,J., Santos,R.,
            Huang,J., Arnold,M., Erte,I., Forgetta,V., Yang,T.P., Walter,K.,
            Menni,C., Chen,L., Vasquez,L., Valdes,A.M., Hyde,C.L., Wang,V.,
            Ziemek,D., Roberts,P., Xi,L., Grundberg,E., Waldenberger,M.,
            Richards,J.B., Mohney,R.P., Milburn,M.V., John,S.L., Trimmer,J.,
            Theis,F.J., Overington,J.P., Suhre,K., Brosnan,M.J., Gieger,C.,
            Kastenmuller,G., Spector,T.D. and Soranzo,N.
  CONSRTM   Multiple Tissue Human Expression Resource (MuTHER) Consortium
  TITLE     An atlas of genetic influences on human blood metabolites
  JOURNAL   Nat Genet 46 (6), 543-550 (2014)
   PUBMED   24816252
REFERENCE   4  (bases 1 to 1349)
  AUTHORS   Okuno,T., Yamabayashi,H. and Kogure,K.
  TITLE     Comparison of intracellular localization of Nubp1 and Nubp2 using
            GFP fusion proteins
  JOURNAL   Mol Biol Rep 37 (3), 1165-1168 (2010)
   PUBMED   19263241
REFERENCE   5  (bases 1 to 1349)
  AUTHORS   Stehling,O., Netz,D.J., Niggemeyer,B., Rosser,R., Eisenstein,R.S.,
            Puccio,H., Pierik,A.J. and Lill,R.
  TITLE     Human Nbp35 is essential for both cytosolic iron-sulfur protein
            assembly and iron homeostasis
  JOURNAL   Mol Cell Biol 28 (17), 5517-5528 (2008)
   PUBMED   18573874
REFERENCE   6  (bases 1 to 1349)
  AUTHORS   Roy,A., Solodovnikova,N., Nicholson,T., Antholine,W. and
            Walden,W.E.
  TITLE     A novel eukaryotic factor for cytosolic Fe-S cluster assembly
  JOURNAL   EMBO J 22 (18), 4826-4835 (2003)
   PUBMED   12970194
REFERENCE   7  (bases 1 to 1349)
  AUTHORS   Nakashima,H., Grahovac,M.J., Mazzarella,R., Fujiwara,H.,
            Kitchen,J.R., Threat,T.A. and Ko,M.S.
  TITLE     Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome
            17, and Nubp1, mapped to chromosome 16--establish a new gene family
            of nucleotide-binding proteins in eukaryotes
  JOURNAL   Genomics 60 (2), 152-160 (1999)
   PUBMED   10486206
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU189242.1, BC002768.2,
            AK300696.1 and BF439443.1.
            
            On Nov 22, 2018 this sequence version replaced NM_012225.3.
            
            Summary: This gene encodes an adenosine triphosphate (ATP) and
            metal-binding protein that is required for the assembly of
            cyotosolic iron-sulfur proteins. The encoded protein functions in a
            heterotetramer with nucleotide-binding protein 1 (NUBP1).
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Oct 2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008005.1, AK001023.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262302.14/ ENSP00000262302.9
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 BU189242.1         38-43
            7-801               BC002768.2         1-795
            802-1245            AK300696.1         573-1016
            1246-1349           BF439443.1         1-104               c
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..1349
                     /gene="NUBP2"
                     /gene_synonym="CFD1; CIAO6; NBP 2; NUBP1"
                     /note="NUBP iron-sulfur cluster assembly factor 2,
                     cytosolic"
                     /db_xref="GeneID:10101"
                     /db_xref="HGNC:HGNC:8042"
                     /db_xref="MIM:610779"
     exon            1..77
                     /gene="NUBP2"
                     /gene_synonym="CFD1; CIAO6; NBP 2; NUBP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..877
                     /gene="NUBP2"
                     /gene_synonym="CFD1; CIAO6; NBP 2; NUBP1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     homolog of yeast cytosolic Fe-S cluster deficient 1;
                     nucleotide binding protein 2 (MinD homolog, E. coli);
                     nucleotide-binding protein 2; cytosolic Fe-S cluster
                     assembly factor NUBP2"
                     /codon_start=1
                     /product="cytosolic Fe-S cluster assembly factor NUBP2
                     isoform 1"
                     /protein_id="NP_036357.1"
                     /db_xref="CCDS:CCDS10445.1"
                     /db_xref="GeneID:10101"
                     /db_xref="HGNC:HGNC:8042"
                     /db_xref="MIM:610779"
                     /translation="MEAAAEPGNLAGVRHIILVLSGKGGVGKSTISTELALALRHAGK
                     KVGILDVDLCGPSIPRMLGAQGRAVHQCDRGWAPVFLDREQSISLMSVGFLLEKPDEA
                     VVWRGPKKNALIKQFVSDVAWGELDYLVVDTPPGTSDEHMATIEALRPYQPLGALVVT
                     TPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHCTECTSVFSRGGGEELAQLA
                     GVPFLGSVPLDPALMRTLEEGHDFIQEFPGSPAFAALTSIAQKILDATPACLP"
     exon            78..196
                     /gene="NUBP2"
                     /gene_synonym="CFD1; CIAO6; NBP 2; NUBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            197..395
                     /gene="NUBP2"
                     /gene_synonym="CFD1; CIAO6; NBP 2; NUBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            396..550
                     /gene="NUBP2"
                     /gene_synonym="CFD1; CIAO6; NBP 2; NUBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            551..661
                     /gene="NUBP2"
                     /gene_synonym="CFD1; CIAO6; NBP 2; NUBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            662..731
                     /gene="NUBP2"
                     /gene_synonym="CFD1; CIAO6; NBP 2; NUBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            732..1349
                     /gene="NUBP2"
                     /gene_synonym="CFD1; CIAO6; NBP 2; NUBP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1320..1325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUBP2"
                     /gene_synonym="CFD1; CIAO6; NBP 2; NUBP1"
                     /note="hexamer: ATTAAA"
     polyA_site      1349
                     /gene="NUBP2"
                     /gene_synonym="CFD1; CIAO6; NBP 2; NUBP1"
                     /note="major polyA site"
ORIGIN      
        1 gggaggctga cggcccgcgg gcgtaagcgg actgcagccg cgagctcctg gaggcggcgg
       61 gatggaggcg gcggccgagc ctggaaacct ggccggcgtc aggcacatca tcctggtcct
      121 ctcaggaaag gggggcgttg ggaaaagcac catctccacg gagctggccc tggcactgcg
      181 ccatgcaggc aagaaggtgg gaatcctgga tgtggacctg tgtggcccca gtatcccccg
      241 catgctcggg gcgcagggca gggctgtgca ccagtgcgac cgcggctggg cacccgtctt
      301 cctggaccgg gagcagagca tctcgctcat gtctgtgggc ttcctgctgg agaagccgga
      361 cgaggccgtg gtgtggagag gccccaagaa aaacgcgctg ataaagcagt ttgtgtccga
      421 cgtggcctgg ggggagctgg actacctggt ggtggacacg cccccgggga cctccgatga
      481 gcacatggcc accatagaag ccctgcgtcc ctaccagccc ctgggggccc tcgtggtcac
      541 cacgccccag gcggtgtccg tgggggacgt gaggcgcgag ctgaccttct gtaggaagac
      601 gggcttgcgg gtgatgggaa tcgtggagaa tatgagcggc ttcacctgcc cacactgcac
      661 ggagtgcacc agcgtcttct ccaggggcgg cggagaggag ctggcccagc tcgccggggt
      721 gcccttctta ggctccgtgc ccctggaccc tgcgctcatg aggaccctgg aggagggcca
      781 cgacttcatc caggagttcc ctgggagccc cgccttcgct gcactcacct ccatagccca
      841 gaagattctg gacgcgacgc ccgcgtgcct cccctgacta aggccacctt gcagccgctt
      901 tccagggcca ccaagggctc tgctccagcc tctcagagaa acagaggcct gggctcggtt
      961 cccgggccct gcaggggcag gcccaggcag cgtcagccgg agagcttctc cccgaccagc
     1021 ccagccccag gatgtgtcgc accagcagct ctgcctggtt ggcctgcagt gccgtggtct
     1081 gcgtgctctg cagctgtgag acgggggcgg cctgggctct cttcccatcc atgttgccta
     1141 cctgtgcccc tggcagccgc gtgtccacac agttagcgga gcgcgggact tctgcagtcc
     1201 tcaggtgacc ccgggcctcc agcaccctgg gtcgctgtca tctgtgttta gctcggggag
     1261 tgccccctaa gggggcgaac tgacctcagg catgttttgt aactgtagag gcgcctgcca
     1321 ttaaacgtgt ccgctgctgt ggcgacaga
//
