LOCUS       NM_001243351            3109 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens negative regulator of ubiquitin like proteins 1
            (NUB1), transcript variant 1, mRNA.
ACCESSION   NM_001243351
VERSION     NM_001243351.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Brockmann,F., Catone,N., Wunsch,C., Offensperger,F., Scheffner,M.,
            Schmidtke,G. and Aichem,A.
  TITLE     FAT10 and NUB1L cooperate to activate the 26S proteasome
  JOURNAL   Life Sci Alliance 6 (8), e202201463 (2023)
   PUBMED   37188463
  REMARK    GeneRIF: FAT10 and NUB1L cooperate to activate the 26S proteasome.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Jiang,W., Zhao,W., Ye,F., Huang,S., Wu,Y., Chen,H., Zhou,R. and
            Fu,G.
  TITLE     SNHG12 regulates biological behaviors of ox-LDL-induced HA-VSMCs
            through upregulation of SPRY2 and NUB1
  JOURNAL   Atherosclerosis 340, 1-11 (2022)
   PUBMED   34847450
  REMARK    GeneRIF: SNHG12 regulates biological behaviors of ox-LDL-induced
            HA-VSMCs through upregulation of SPRY2 and NUB1.
REFERENCE   3  (bases 1 to 3109)
  AUTHORS   Hosono,T., Tanaka,T., Tanji,K., Nakatani,T. and Kamitani,T.
  TITLE     NUB1, an interferon-inducible protein, mediates anti-proliferative
            actions and apoptosis in renal cell carcinoma cells through
            cell-cycle regulation
  JOURNAL   Br J Cancer 102 (5), 873-882 (2010)
   PUBMED   20160729
REFERENCE   4  (bases 1 to 3109)
  AUTHORS   Tanji,K., Mori,F., Kakita,A., Zhang,H., Kito,K., Kamitani,T.,
            Takahashi,H. and Wakabayashi,K.
  TITLE     Immunohistochemical localization of NUB1, a synphilin-1-binding
            protein, in neurodegenerative disorders
  JOURNAL   Acta Neuropathol 114 (4), 365-371 (2007)
   PUBMED   17549501
REFERENCE   5  (bases 1 to 3109)
  AUTHORS   van der Spuy,J. and Cheetham,M.E.
  TITLE     The Leber congenital amaurosis protein AIPL1 modulates the nuclear
            translocation of NUB1 and suppresses inclusion formation by NUB1
            fragments
  JOURNAL   J Biol Chem 279 (46), 48038-48047 (2004)
   PUBMED   15347646
  REMARK    GeneRIF: Data show that aryl hydrocarbon receptor-interacting
            protein-like 1 (AIPL1) can modulate protein translocation and act
            in a chaperone-like manner and suggest that AIPL1 is an important
            modulator of NEDD8 ultimate buster protein 1 (NUB1) cellular
            function.
REFERENCE   6  (bases 1 to 3109)
  AUTHORS   Kanaya,K., Sohocki,M.M. and Kamitani,T.
  TITLE     Abolished interaction of NUB1 with mutant AIPL1 involved in Leber
            congenital amaurosis
  JOURNAL   Biochem Biophys Res Commun 317 (3), 768-773 (2004)
   PUBMED   15081406
  REMARK    GeneRIF: interaction between NUB1 and AIPL1 is affected in patients
            with Leber congenital amaurosis
REFERENCE   7  (bases 1 to 3109)
  AUTHORS   Tanaka,T., Kawashima,H., Yeh,E.T. and Kamitani,T.
  TITLE     Regulation of the NEDD8 conjugation system by a splicing variant,
            NUB1L
  JOURNAL   J Biol Chem 278 (35), 32905-32913 (2003)
   PUBMED   12816948
  REMARK    GeneRIF: NUB1 has a splicing variant, NUB1L, which regulates the
            NEDD8 conjugation system
REFERENCE   8  (bases 1 to 3109)
  AUTHORS   Kamitani,T., Kito,K., Fukuda-Kamitani,T. and Yeh,E.T.
  TITLE     Targeting of NEDD8 and its conjugates for proteasomal degradation
            by NUB1
  JOURNAL   J Biol Chem 276 (49), 46655-46660 (2001)
   PUBMED   11585840
REFERENCE   9  (bases 1 to 3109)
  AUTHORS   Kito,K., Yeh,E.T. and Kamitani,T.
  TITLE     NUB1, a NEDD8-interacting protein, is induced by interferon and
            down-regulates the NEDD8 expression
  JOURNAL   J Biol Chem 276 (23), 20603-20609 (2001)
   PUBMED   11259415
REFERENCE   10 (bases 1 to 3109)
  AUTHORS   Scanlan,M.J., Gordan,J.D., Williamson,B., Stockert,E., Bander,N.H.,
            Jongeneel,V., Gure,A.O., Jager,D., Jager,E., Knuth,A., Chen,Y.T.
            and Old,L.J.
  TITLE     Antigens recognized by autologous antibody in patients with
            renal-cell carcinoma
  JOURNAL   Int J Cancer 83 (4), 456-464 (1999)
   PUBMED   10508479
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005486.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 16, 2020 this sequence version replaced NM_001243351.1.
            
            Summary: This gene encodes a protein that functions as a negative
            regulator of NEDD8, a ubiquitin-like protein that conjugates with
            cullin family members in order to regulate vital biological events.
            The protein encoded by this gene regulates the NEDD8 conjugation
            system post-transcriptionally by recruiting NEDD8 and its
            conjugates to the proteasome for degradation. This protein
            interacts with the product of the AIPL1 gene, which is associated
            with Leber congenital amaurosis, an inherited retinopathy, and
            mutations in that gene can abolish interaction with this protein,
            which may contribute to the pathogenesis. This protein is also
            known to accumulate in Lewy bodies in Parkinson's disease and
            dementia with Lewy bodies, and in glial cytoplasmic inclusions in
            multiple system atrophy, with this abnormal accumulation being
            specific to alpha-synucleinopathy lesions. Alternative splicing of
            this gene results in multiple transcript variants. [provided by
            RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (2, also known as NUB1L).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF459743.1, SRR18074967.3364852.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000568733.6/ ENSP00000454264.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AC005486.2         56424-56458         c
            36-154              AC005486.2         52804-52922         c
            155-322             AC005486.2         49030-49197         c
            323-381             AC005486.2         46788-46846         c
            382-452             AC005486.2         45388-45458         c
            453-635             AC005486.2         42320-42502         c
            636-730             AC005486.2         42048-42142         c
            731-837             AC005486.2         38023-38129         c
            838-1024            AC005486.2         31145-31331         c
            1025-1132           AC005486.2         30302-30409         c
            1133-1285           AC005486.2         29383-29535         c
            1286-1432           AC005486.2         24027-24173         c
            1433-1528           AC005486.2         22327-22422         c
            1529-1706           AC005486.2         21459-21636         c
            1707-3109           AC005486.2         19821-21223         c
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q36.1"
     gene            1..3109
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /note="negative regulator of ubiquitin like proteins 1"
                     /db_xref="GeneID:51667"
                     /db_xref="HGNC:HGNC:17623"
                     /db_xref="MIM:607981"
     exon            1..35
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            36..154
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..1885
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /note="isoform 1 is encoded by transcript variant 1; NEDD8
                     ultimate buster-1; renal carcinoma antigen NY-REN-18"
                     /codon_start=1
                     /product="NEDD8 ultimate buster 1 isoform 1"
                     /protein_id="NP_001230280.2"
                     /db_xref="CCDS:CCDS87565.1"
                     /db_xref="GeneID:51667"
                     /db_xref="HGNC:HGNC:17623"
                     /db_xref="MIM:607981"
                     /translation="MAQKKYLQAKLTQFLREDRIQLWKPPYTDENKKVGLALKDLAKQ
                     YSDRLECCENEVEKVIEEIRCKAIERGTGNDNYRTTGIATIEVFLPPRLKKDRKNLLE
                     TRLHITGRELRSKIAETFGLQENYIKIVINKKQLQLGKTLEEQGVAHNVKAMVLELKQ
                     SEEDARKNFQLEEEEQNEAKLKEKQIQRTKRGLEILAKRAAETVVDPEMTPYLDIANQ
                     TGRSIRIPPSERKALMLAMGYHEKGRAFLKRKEYGIALPCLLDADKYFCECCRELLDT
                     VDNYAVLQLDIVWCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGK
                     EKVLFLRLYLLQGIRNYHSGNDVEAYEYLNKARQLFKELYIDPSKVDNLLQLGFTAQE
                     ARLGLRACDGNVDHAATHITNRREELAQIRKEEKEKKRRRLENIRFLKGMGYSTHAAQ
                     QVLHAASGNLDEALKILLSNPQMWWLNDSNPETDNRQESPSQENIDRLVYMGFDALVA
                     EAALRVFRGNVQLAAQTLAHNGGSLPPELPLSPEDSLSPPATSPSDSAGTSSASTDED
                     METEAVNEILEDIPEHEEDYLDSTLEDEEIIIAEYLSYVENRKSATKKN"
     exon            155..322
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            323..381
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            382..452
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            453..635
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            636..730
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            731..837
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            838..1024
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1132
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1285
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1432
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1528
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1706
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..3109
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3082..3087
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /note="hexamer: AATAAA"
     polyA_site      3109
                     /gene="NUB1"
                     /gene_synonym="BS4; NUB1L; NYREN18"
                     /note="major polyA site"
ORIGIN      
        1 actctgctgg tcgcggcggg agtggcgtgg cgcagggatg gcacaaaaga aatatcttca
       61 agcaaaattg acccagtttt taagggaaga caggattcaa ctttggaaac ctccatatac
      121 agatgaaaat aaaaaagttg gtttggcatt aaaggacctt gctaagcagt actctgacag
      181 actagaatgc tgtgaaaatg aagtagaaaa ggtaatagaa gaaatacgtt gcaaggcaat
      241 tgagcgtgga acaggaaatg acaattatag aacaacggga attgctacaa tcgaggtgtt
      301 tttaccacca agactaaaaa aagataggaa aaacttgttg gagacccgat tgcacatcac
      361 tggcagagaa ctgaggtcca aaatagctga aacctttgga cttcaagaaa attatatcaa
      421 aattgtcata aataagaagc aactacaact agggaaaacc cttgaagaac aaggcgtggc
      481 tcacaatgtg aaagcgatgg tgcttgaact aaaacaatct gaagaggacg cgaggaaaaa
      541 cttccagtta gaggaagagg agcaaaatga ggccaaactc aaagaaaaac aaattcagag
      601 gaccaagaga ggactagaaa tactggcaaa gagagcagca gagacagtgg tggatccaga
      661 aatgacaccg tacttagaca tagctaacca gacaggcaga tcaatcagaa ttcccccatc
      721 agaaagaaaa gcccttatgt tagctatggg atatcatgag aagggcagag ctttcctgaa
      781 aagaaaagaa tatggaatag ccttgccatg tctgttggac gctgacaaat atttctgtga
      841 gtgttgcaga gagctgctgg acacagtgga taactacgcc gtcctccagc tggatatagt
      901 gtggtgttac ttccgcctgg aacagctgga atgccttgat gatgcagaaa aaaaattaaa
      961 cttggcccag aaatgcttta aaaattgtta cggagaaaat catcagagac tggtccacat
     1021 aaaaggaaat tgtgggaaag agaaggtact gtttctaaga ctctacttac ttcaagggat
     1081 ccgaaactat cacagtggaa atgatgtaga ggcttatgag tatcttaaca aggcacgtca
     1141 gctctttaaa gagctatata ttgatccatc aaaagtggac aatttgttgc agttggggtt
     1201 tactgcccag gaagcccggc ttggcctgag ggcgtgtgat gggaacgtgg atcatgcggc
     1261 cactcatatt accaaccgca gagaggaact ggcccaaata aggaaggagg aaaaagagaa
     1321 gaaaagacgc cgcctcgaga acatcaggtt tctgaaaggg atgggctact ccacgcacgc
     1381 ggcccagcag gtactccacg cagccagcgg gaacttggat gaggccctga agattctgct
     1441 cagcaatcct cagatgtggt ggttaaatga ttccaatcct gaaaccgaca accgtcaaga
     1501 aagtccttcc caggaaaaca ttgaccgatt ggtgtacatg ggttttgatg cactcgtggc
     1561 cgaagctgcg ctgagagtgt tcagaggcaa cgtccagctg gccgcccaga cccttgctca
     1621 caacggagga agcctgcctc ccgagctgcc gctgtcgcca gaagactctt tgtccccgcc
     1681 agccacgtcc ccttctgact ccgcaggaac ctctagtgcc tcaacagacg aagacatgga
     1741 gacagaggcc gtcaatgaga tactggaaga cattccagag catgaggaag actatcttga
     1801 ctcaactctg gaagatgaag aaattattat tgcagagtac ctatcctatg tagaaaatag
     1861 gaagtcagca acaaagaaaa actaaataat gaacagaaat agcgctaatt ttctgcttat
     1921 aaatgctatc attatgaaaa ggctaatgca gctctttctg ttcttacttt ttatctgaat
     1981 tacaagtcct ctttgggtgt aggagggggt gggcagggga caagtccagg aggggtccca
     2041 gggccttcat gcgtggtctc ggggaagaag cttcctctgg cctggcgcag gccgttccat
     2101 ctgcctccca ggtctgcgtc cctaacccct tccccagctt ggtgttttac cccgaaacag
     2161 gaaggaacag gggtcctgta gaacaggggt cctggggaag gtgtccaggg cagggtcctg
     2221 ggaagggtgt cccgaccgct tcctctccag ctgtggctcc atctgcccag cttgcctgcc
     2281 tcctgcaccc actgccctga ccttcctgct tcccacgctg ccatctctgc cagggtgcca
     2341 catgggttcc tgtgccaccc tttccccgcc cctcaaatcg tcctttaagt cttccttcca
     2401 agtgctgtgg ggcataacga tgaggcgctg gccttggggg ccacaccagg tcgcagcaaa
     2461 tggcttcagc ctgggacgcc agtgttttat gctcttagtt cagtaaaata cgcccccgaa
     2521 attcaagatt gagtgtcagg ctttatatat attcagcatt cctcattaca gaaatcttct
     2581 attgaatggg aaaggtttaa atgctaacca aagcaattta tttttaatta atatttttag
     2641 actctgtgct gtcatactga actcactgct agctaagaga cctatcagag atttagatat
     2701 attttctcca ggttttttgt gggttttctt tgttgttgtt gttgttctag ccatgtgaca
     2761 gaggctcttt ctaaaagtat gtagttcgct gtgtgtcggc tccagcagta accgtcctca
     2821 ctgcgccacg cactcctctg tagatgtgtg cccagtggga gttccttcca gccccaggac
     2881 cacagcagca gccaggtgcc gagtggattg agtgccaggt gcatccaaga ctttccctcc
     2941 cttccagaag gcactgactg aagacaggat ggatcatgcg gagccggctg aaatgctcca
     3001 actttttcaa agtgtgggtg gtccagtttg gactgatggg aatcttcttg tcattctttt
     3061 taaacggatg ataccgatgg aaataaaagg tgggaaatat attcaaaaa
//
