LOCUS       NM_021229               3621 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens netrin 4 (NTN4), transcript variant 1, mRNA.
ACCESSION   NM_021229
VERSION     NM_021229.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Vennela,J., Pottakkat,B., Vairappan,B.S., Verma,S.K. and
            Mukherjee,V.
  TITLE     Hepatic Expression of NTN4 and Its Receptors in Patients with
            Hepatocellular Carcinoma
  JOURNAL   Asian Pac J Cancer Prev 24 (12), 4285-4292 (2023)
   PUBMED   38156865
  REMARK    GeneRIF: Hepatic Expression of NTN4 and Its Receptors in Patients
            with Hepatocellular Carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Wu,Z., Liu,S., Pang,G. and Jiang,H.
  TITLE     RNA-binding protein quaking 5 inhibits the progression of non-small
            cell lung cancer by upregulating netrin-4 expression
  JOURNAL   Oncol Rep 50 (5) (2023)
   PUBMED   37800632
  REMARK    GeneRIF: RNA-binding protein quaking 5 inhibits the progression of
            non-small cell lung cancer by upregulating netrin-4 expression.
REFERENCE   3  (bases 1 to 3621)
  AUTHORS   Dong,F., Liu,Y., Yan,W., Meng,Q., Song,X., Cheng,B. and Yao,R.
  TITLE     Netrin-4: Focus on Its Role in Axon Guidance, Tissue Stability,
            Angiogenesis and Tumors
  JOURNAL   Cell Mol Neurobiol 43 (5), 1663-1683 (2023)
   PUBMED   36350538
  REMARK    GeneRIF: Netrin-4: Focus on Its Role in Axon Guidance, Tissue
            Stability, Angiogenesis and Tumors.
            Review article
REFERENCE   4  (bases 1 to 3621)
  AUTHORS   Hayano,Y., Sasaki,K., Ohmura,N., Takemoto,M., Maeda,Y.,
            Yamashita,T., Hata,Y., Kitada,K. and Yamamoto,N.
  TITLE     Netrin-4 regulates thalamocortical axon branching in an
            activity-dependent fashion
  JOURNAL   Proc Natl Acad Sci U S A 111 (42), 15226-15231 (2014)
   PUBMED   25288737
  REMARK    GeneRIF: NTN4 acts as a positive regulator for TC axon branching
            through activity-dependent expression
REFERENCE   5  (bases 1 to 3621)
  AUTHORS   Lejmi,E., Leconte,L., Pedron-Mazoyer,S., Ropert,S., Raoul,W.,
            Lavalette,S., Bouras,I., Feron,J.G., Maitre-Boube,M., Assayag,F.,
            Feumi,C., Alemany,M., Jie,T.X., Merkulova,T., Poupon,M.F.,
            Ruchoux,M.M., Tobelem,G., Sennlaub,F. and Plouet,J.
  TITLE     Netrin-4 inhibits angiogenesis via binding to neogenin and
            recruitment of Unc5B
  JOURNAL   Proc Natl Acad Sci U S A 105 (34), 12491-12496 (2008)
   PUBMED   18719102
  REMARK    GeneRIF: Knockdown of Netrin-4 expression in EC increased their
            ability to form tubular structures; Netrin-4 bound only to neogenin
            but not to Unc5B or Unc5C receptors
REFERENCE   6  (bases 1 to 3621)
  AUTHORS   Esseghir,S., Kennedy,A., Seedhar,P., Nerurkar,A., Poulsom,R.,
            Reis-Filho,J.S. and Isacke,C.M.
  TITLE     Identification of NTN4, TRA1, and STC2 as prognostic markers in
            breast cancer in a screen for signal sequence encoding proteins
  JOURNAL   Clin Cancer Res 13 (11), 3164-3173 (2007)
   PUBMED   17545519
  REMARK    GeneRIF: NTN4, TRA1, and STC2 have roles in progression of breast
            cancer
REFERENCE   7  (bases 1 to 3621)
  AUTHORS   Qin,S., Yu,L., Gao,Y., Zhou,R. and Zhang,C.
  TITLE     Characterization of the receptors for axon guidance factor netrin-4
            and identification of the binding domains
  JOURNAL   Mol Cell Neurosci 34 (2), 243-250 (2007)
   PUBMED   17174565
REFERENCE   8  (bases 1 to 3621)
  AUTHORS   Zhang,C., Meng,F., Wang,C., Guo,H., Fan,M., Liu,S., Zhou,R. and
            He,F.
  TITLE     Identification of a novel alternative splicing form of human
            netrin-4 and analyzing the expression patterns in adult rat brain
  JOURNAL   Brain Res Mol Brain Res 130 (1-2), 68-80 (2004)
   PUBMED   15519678
REFERENCE   9  (bases 1 to 3621)
  AUTHORS   Koch,M., Murrell,J.R., Hunter,D.D., Olson,P.F., Jin,W., Keene,D.R.,
            Brunken,W.J. and Burgeson,R.E.
  TITLE     A novel member of the netrin family, beta-netrin, shares homology
            with the beta chain of laminin: identification, expression, and
            functional characterization
  JOURNAL   J Cell Biol 151 (2), 221-234 (2000)
   PUBMED   11038171
REFERENCE   10 (bases 1 to 3621)
  AUTHORS   Genini,M., Schwalbe,P., Scholl,F.A. and Schafer,B.W.
  TITLE     Isolation of genes differentially expressed in human primary
            myoblasts and embryonal rhabdomyosarcoma
  JOURNAL   Int J Cancer 66 (4), 571-577 (1996)
   PUBMED   8635876
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC090001.19, BI549738.1,
            AF297711.1, AL833767.1 and BC013591.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_021229.3.
            
            Summary: This gene encodes a member of the netrin family of
            proteins, which function in various biological processes including
            axon guidance, tumorogenesis, and angiogenesis. Netrins are
            laminin-related proteins that have an N-terminal laminin-type
            domain, epidermal growth factor-like repeat domain, and a
            positively charged heparin-binding domain at the C-terminus. The
            protein encoded by this gene is involved in processes including
            neurite growth and migration, angiogenesis and mural cell adhesion
            to endothelial cells. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Jul 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF278532.1, AF297711.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000343702.9/ ENSP00000340998.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AC090001.19        103000-103005       c
            7-353               BI549738.1         12-358
            354-1388            AF297711.1         200-1234
            1389-2480           AL833767.1         787-1878
            2481-3318           BC013591.1         1550-2387
            3319-3621           AL833767.1         2717-3019
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q22"
     gene            1..3621
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /note="netrin 4"
                     /db_xref="GeneID:59277"
                     /db_xref="HGNC:HGNC:13658"
                     /db_xref="MIM:610401"
     exon            1..510
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /inference="alignment:Splign:2.1.0"
     CDS             456..2342
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; beta-netrin; hepar-derived netrin-like protein"
                     /codon_start=1
                     /product="netrin-4 isoform 1 precursor"
                     /protein_id="NP_067052.2"
                     /db_xref="CCDS:CCDS9054.1"
                     /db_xref="GeneID:59277"
                     /db_xref="HGNC:HGNC:13658"
                     /db_xref="MIM:610401"
                     /translation="MGSCARLLLLWGCTVVAAGLSGVAGVSSRCEKACNPRMGNLALG
                     RKLWADTTCGQNATELYCFYSENTDLTCRQPKCDKCNAAYPHLAHLPSAMADSSFRFP
                     RTWWQSAEDVHREKIQLDLEAEFYFTHLIVMFKSPRPAAMVLDRSQDFGKTWKPYKYF
                     ATNCSATFGLEDDVVKKGAICTSKYSSPFPCTGGEVIFKALSPPYDTENPYSAKVQEQ
                     LKITNLRVQLLKRQSCPCQRNDLNEEPQHFTHYAIYDFIVKGSCFCNGHADQCIPVHG
                     FRPVKAPGTFHMVHGKCMCKHNTAGSHCQHCAPLYNDRPWEAADGKTGAPNECRTCKC
                     NGHADTCHFDVNVWEASGNRSGGVCDDCQHNTEGQYCQRCKPGFYRDLRRPFSAPDAC
                     KPCSCHPVGSAVLPANSVTFCDPSNGDCPCKPGVAGRRCDRCMVGYWGFGDYGCRPCD
                     CAGSCDPITGDCISSHTDIDWYHEVPDFRPVHNKSEPAWEWEDAQGFSALLHSGKCEC
                     KEQTLGNAKAFCGMKYSYVLKIKILSAHDKGTHVEVNVKIKKVLKSTKLKIFRGKRTL
                     YPESWTDRGCTCPILNPGLEYLVAGHEDIRTGKLIVNMKSFVQHWKPSLGRKVMDILK
                     RECK"
     exon            511..1040
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1319
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1446
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1635
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1849
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..1965
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2034
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2205
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /inference="alignment:Splign:2.1.0"
     exon            2206..3621
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3602..3607
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /note="hexamer: AATAAA"
     polyA_site      3621
                     /gene="NTN4"
                     /gene_synonym="PRO3091"
                     /note="major polyA site"
ORIGIN      
        1 ggagaaggac gggacggagc cggggcagcc agaagaggtg ggaaaagcgg aggaggacgc
       61 ccaggaggag gcggcggcgg cggccgggaa gtgaaaggtc tcgcaaagtt cagcggcggc
      121 tgcgggcgcc gagccccggg ctagcggcag acgagcccgc agggccgctc cgcggggcag
      181 cgcagccagg ccggctatgg tcccggggct cccgccgccc cccaggtgcc cgggacccgc
      241 caggccggtg cgcgagggtc accccacctc cccgcgcggt cccggcccct ggctcccagc
      301 tgccggcgac cgctgaccga gcccggcgcc ccaggaggag gaagaaacca gggccccgtt
      361 ccctcccgag gacggcggcg cttcatcccg cagcccagag gtctcggctc cctccggcac
      421 ccgcccggcc cggctgctcc cggctcctcc cggccatggg gagctgcgcg cggctgctgc
      481 tgctctgggg ctgcacggtg gtggccgcag gactgagtgg agtagctgga gtgagttccc
      541 gctgtgaaaa agcctgcaac cctcggatgg gaaatttggc tttggggcga aaactctggg
      601 cagacaccac ctgcggtcag aatgctaccg aactgtactg cttctacagt gagaacacgg
      661 atctgacttg tcggcagccc aaatgtgaca agtgcaatgc tgcctatcct cacctggctc
      721 acctgccatc tgccatggca gactcatcct tccggtttcc tcgcacatgg tggcagtctg
      781 cggaggatgt gcacagagaa aagatccagt tagacctgga agctgaattc tacttcactc
      841 acctaattgt gatgttcaag tcccccaggc cggctgccat ggtgctggac cgctcccagg
      901 actttgggaa aacatggaag ccttataagt actttgcgac taactgctcc gctacatttg
      961 gcctggaaga tgatgttgtc aagaagggcg ctatttgtac ttctaaatac tccagtcctt
     1021 ttccatgcac tggaggagag gttattttca aagctttgtc accaccatac gatacagaga
     1081 acccttacag tgccaaagtt caggagcagc tgaagatcac caaccttcgc gtgcagctgc
     1141 tgaaacgaca gtcttgtccc tgtcagagaa atgacctgaa cgaagagcct caacatttta
     1201 cacactatgc aatctatgat ttcattgtca agggcagctg cttctgcaat ggccacgctg
     1261 atcaatgcat acctgttcat ggcttcagac ctgtcaaggc cccaggaaca ttccacatgg
     1321 tccatgggaa gtgtatgtgt aagcacaaca cagcaggcag ccactgccag cactgtgccc
     1381 cgttatacaa tgaccggcca tgggaggcag ctgatggcaa aacgggggct cccaacgagt
     1441 gcagaacctg caagtgtaat gggcatgctg atacctgtca cttcgacgtt aatgtgtggg
     1501 aggcatcagg gaatcgtagt ggtggtgtct gtgatgactg tcagcacaac acagaaggac
     1561 agtattgcca gaggtgcaag ccaggcttct atcgtgacct gcggagaccc ttctcagctc
     1621 cagatgcttg caaaccgtgt tcctgccatc cagtaggatc agctgtcctt cctgccaact
     1681 cagtgacctt ctgcgacccc agcaatggtg actgcccttg caagcctggg gtggcagggc
     1741 gacgttgtga caggtgcatg gtgggatact ggggcttcgg agactatggc tgtcgaccat
     1801 gtgactgtgc ggggagctgt gaccctatca ccggagactg catcagcagc cacacagaca
     1861 tagactggta tcatgaagtt cctgacttcc gtcccgtgca caataagagc gaaccagcct
     1921 gggagtggga ggatgcgcag gggttttctg cacttctaca ctcaggtaaa tgcgaatgta
     1981 aggaacagac attaggaaat gccaaggcat tctgtggaat gaaatattca tatgtgctaa
     2041 aaataaagat tttatcagct catgataaag gtactcatgt tgaggtcaat gtgaagatta
     2101 aaaaggtctt aaaatctacc aaactgaaga ttttccgagg aaagcgaaca ttatatccag
     2161 aatcatggac ggacagagga tgcacttgtc caatcctcaa tcctggtttg gaataccttg
     2221 tagcaggaca tgaggatata agaacaggca aactaattgt gaatatgaaa agctttgtcc
     2281 agcactggaa accttctctt ggaagaaaag tcatggatat tttaaaaaga gagtgcaagt
     2341 agcattaaga tggatagcac ataatggcac ttgtctatgt acaaaacaca aactttagag
     2401 caagaagacc tcagacagga aactggaatt ttttaaagtg ccaaaacata tagaaatgtt
     2461 tgaatgcatg ggtcttatct aacttatctc ttctggaccc atgtttaaat acagttttat
     2521 ttcatgaaga gaaatgaaaa cccctacact gatatctgtt ttctatggga ctgattctga
     2581 aattcttaac tattaagaat attttaatag cagcatgaca tttagcagta atccattaag
     2641 ggcagtacct ctaacaagga cgccttccag cttcagcgat gttacttacg tttgatgcta
     2701 cttaaagtaa tgaatgacgt tttaaggaat ccctaaccct actatcagaa aaggtgtttg
     2761 ttaaagagcc ttctcttgtg tgttacgcat gaactttggt ctgtaggtgt taaatggaac
     2821 ctctccatgt gtatatagta tttccttgta taaagcactt tactacctac cacttgtgtt
     2881 gtgaacgttt ggtgactgct gttgaaagaa ggaaaagggt gtgtgagaaa gcctactgaa
     2941 gcagcagcac tgccactaca tgtggacaaa agtgaccata taaaagaagt tgtgctattt
     3001 aactctgaat acttggagaa actaggtgaa gatgcaacca gaaaggagaa tatgtatgcg
     3061 tgaagtctca gctttgagct ggaggctaga ttccaagatg acagccatga tgaaactttt
     3121 taaaaaacta aaccagaaga gactttaaaa taagagaaag aaatcataaa tgtagacata
     3181 tgcttggcta aaggggaaat ggactttaaa ttttaaagag ctcatttgca atgcacttgt
     3241 atacacttca aaaattattg tagacacaga atttgttata tttttgtgct tagtatttaa
     3301 acctgaacat tgaaacagtt ttcctccttg tctttcttaa cagtaatagt cattatattt
     3361 acctgttttt taacacaatg tatgtgatag tcaaaaaatc acagtttttc attattattc
     3421 atcttctgta cccacgcata accactatac atagtttctt ttgtacttga atatacaaaa
     3481 catgaacaca gtgccatatg aataatttca catacagaac ctttttttct ctgaagtcct
     3541 gtggacttgc aaatatatat atatattgct ttgttaattt gtttttatat ttcatatatg
     3601 taataaagga atatgatctg a
//
