LOCUS       NM_002526               3562 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens 5'-nucleotidase ecto (NT5E), transcript variant 1,
            mRNA.
ACCESSION   NM_002526
VERSION     NM_002526.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Zhu,J., Du,W., Zeng,Y., Liu,T., Li,J., Wang,A., Li,Y., Zhang,W.,
            Huang,J.A. and Liu,Z.
  TITLE     CD73 promotes non-small cell lung cancer metastasis by regulating
            Axl signaling independent of GAS6
  JOURNAL   Proc Natl Acad Sci U S A 121 (43), e2404709121 (2024)
   PUBMED   39423241
  REMARK    GeneRIF: CD73 promotes non-small cell lung cancer metastasis by
            regulating Axl signaling independent of GAS6.
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Zhan,J., Huang,L., Niu,L., Lu,W., Sun,C., Liu,S., Ding,Z. and Li,E.
  TITLE     Regulation of CD73 on NAD metabolism: Unravelling the interplay
            between tumour immunity and tumour metabolism
  JOURNAL   Cell Commun Signal 22 (1), 387 (2024)
   PUBMED   39090604
  REMARK    GeneRIF: Regulation of CD73 on NAD metabolism: Unravelling the
            interplay between tumour immunity and tumour metabolism.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3562)
  AUTHORS   Giraulo,C., Orlando,L., Morretta,E., Voli,A., Plaitano,P.,
            Cicala,C., Potaptschuk,E., Muller,C.E., Tosco,A., Monti,M.C. and
            Morello,S.
  TITLE     High levels of soluble CD73 unveil resistance to BRAF inhibitors in
            melanoma cells
  JOURNAL   Biomed Pharmacother 177, 117033 (2024)
   PUBMED   38941889
  REMARK    GeneRIF: High levels of soluble CD73 unveil resistance to BRAF
            inhibitors in melanoma cells.
REFERENCE   4  (bases 1 to 3562)
  AUTHORS   Rubenich,D.S., Domagalski,J.L., Gentil,G.F.S., Eichberger,J.,
            Fiedler,M., Weber,F., Federlin,M., Poeck,H., Reichert,T.E.,
            Ettl,T., Bauer,R.J., Braganhol,E. and Schulz,D.
  TITLE     The immunomodulatory ballet of tumour-derived extracellular
            vesicles and neutrophils orchestrating the dynamic CD73/PD-L1
            pathway in cancer
  JOURNAL   J Extracell Vesicles 13 (7), e12480 (2024)
   PUBMED   38978304
  REMARK    GeneRIF: The immunomodulatory ballet of tumour-derived
            extracellular vesicles and neutrophils orchestrating the dynamic
            CD73/PD-L1 pathway in cancer.
REFERENCE   5  (bases 1 to 3562)
  AUTHORS   Hansen,K.R., Resta,R., Webb,C.F. and Thompson,L.F.
  TITLE     Isolation and characterization of the promoter of the human
            5'-nucleotidase (CD73)-encoding gene
  JOURNAL   Gene 167 (1-2), 307-312 (1995)
   PUBMED   8566797
REFERENCE   6  (bases 1 to 3562)
  AUTHORS   Klemens,M.R., Sherman,W.R., Holmberg,N.J., Ruedi,J.M., Low,M.G. and
            Thompson,L.F.
  TITLE     Characterization of soluble vs membrane-bound human placental
            5'-nucleotidase
  JOURNAL   Biochem Biophys Res Commun 172 (3), 1371-1377 (1990)
   PUBMED   2173922
REFERENCE   7  (bases 1 to 3562)
  AUTHORS   Misumi,Y., Ogata,S., Ohkubo,K., Hirose,S. and Ikehara,Y.
  TITLE     Primary structure of human placental 5'-nucleotidase and
            identification of the glycolipid anchor in the mature form
  JOURNAL   Eur J Biochem 191 (3), 563-569 (1990)
   PUBMED   2129526
REFERENCE   8  (bases 1 to 3562)
  AUTHORS   Thomson,L.F., Ruedi,J.M., Glass,A., Moldenhauer,G., Moller,P.,
            Low,M.G., Klemens,M.R., Massaia,M. and Lucas,A.H.
  TITLE     Production and characterization of monoclonal antibodies to the
            glycosyl phosphatidylinositol-anchored lymphocyte differentiation
            antigen ecto-5'-nucleotidase (CD73)
  JOURNAL   Tissue Antigens 35 (1), 9-19 (1990)
   PUBMED   2137649
REFERENCE   9  (bases 1 to 3562)
  AUTHORS   Boyle,J.M., Hey,Y., Guerts van Kessel,A. and Fox,M.
  TITLE     Assignment of ecto-5'-nucleotidase to human chromosome 6
  JOURNAL   Hum Genet 81 (1), 88-92 (1988)
   PUBMED   2848759
REFERENCE   10 (bases 1 to 3562)
  AUTHORS   Stefanovic,V., Mandel,P. and Rosenberg,A.
  TITLE     Ecto-5'-nucleotidase of intact cultured C6 rat glioma cells
  JOURNAL   J Biol Chem 251 (13), 3900-3905 (1976)
   PUBMED   819433
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA574510.1, X55740.1,
            BC065937.1, AK075008.1 and BQ019358.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002526.3.
            
            Summary: The protein encoded by this gene is a plasma membrane
            protein that catalyzes the conversion of extracellular nucleotides
            to membrane-permeable nucleosides. The encoded protein is used as a
            determinant of lymphocyte differentiation. Defects in this gene can
            lead to the calcification of joints and arteries. Two transcript
            variants encoding different isoforms have been found for this
            gene.[provided by RefSeq, Mar 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.113339.1, X55740.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000257770.8/ ENSP00000257770.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 DA574510.1         500-507
            9-2570              X55740.1           1-2562
            2571-2720           BC065937.1         2539-2688
            2721-3335           X55740.1           2712-3326
            3336-3560           AK075008.1         3160-3384
            3561-3562           BQ019358.1         26-27               c
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q14.3"
     gene            1..3562
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /note="5'-nucleotidase ecto"
                     /db_xref="GeneID:4907"
                     /db_xref="HGNC:HGNC:8021"
                     /db_xref="MIM:129190"
     exon            1..396
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..1782
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.91"
                     /EC_number="3.1.3.89"
                     /EC_number="3.1.3.99"
                     /EC_number="3.1.3.35"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; Purine 5-Prime-Nucleotidase;
                     ecto-5'-nucleotidase; 5'-NT; 5'-deoxynucleotidase;
                     thymidylate 5'-phosphatase; IMP-specific 5'-nucleotidase"
                     /codon_start=1
                     /product="5'-nucleotidase isoform 1 preproprotein"
                     /protein_id="NP_002517.1"
                     /db_xref="CCDS:CCDS5002.1"
                     /db_xref="GeneID:4907"
                     /db_xref="HGNC:HGNC:8021"
                     /db_xref="MIM:129190"
                     /translation="MCPRAARAPATLLLALGAVLWPAAGAWELTILHTNDVHSRLEQT
                     SEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVA
                     HFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLP
                     YKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIAL
                     GHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVP
                     VVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNY
                     STQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGG
                     GIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFL
                     QVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGF
                     QMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFSTGSHCHGSFSLIFLSLW
                     AVIFVLYQ"
     exon            397..619
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /inference="alignment:Splign:2.1.0"
     exon            620..808
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /inference="alignment:Splign:2.1.0"
     exon            809..1006
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1161
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1267
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1417
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1618
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..3562
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3542..3547
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /note="hexamer: AATAAA"
     polyA_site      3562
                     /gene="NT5E"
                     /gene_synonym="CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE"
                     /note="major polyA site"
ORIGIN      
        1 actcgccggc actcgcccgg ctcgcccgct ttcgcaccca gttcacgcgc cacagctatg
       61 tgtccccgag ccgcgcgggc gcccgcgacg ctactcctcg ccctgggcgc ggtgctgtgg
      121 cctgcggctg gcgcctggga gcttacgatt ttgcacacca acgacgtgca cagccggctg
      181 gagcagacca gcgaggactc cagcaagtgc gtcaacgcca gccgctgcat gggtggcgtg
      241 gctcggctct tcaccaaggt tcagcagatc cgccgcgccg aacccaacgt gctgctgctg
      301 gacgccggcg accagtacca gggcactatc tggttcaccg tgtacaaggg cgccgaggtg
      361 gcgcacttca tgaacgccct gcgctacgat gccatggcac tgggaaatca tgaatttgat
      421 aatggtgtgg aaggactgat cgagccactc ctcaaagagg ccaaatttcc aattctgagt
      481 gcaaacatta aagcaaaggg gccactagca tctcaaatat caggacttta tttgccatat
      541 aaagttcttc ctgttggtga tgaagttgtg ggaatcgttg gatacacttc caaagaaacc
      601 ccttttctct caaatccagg gacaaattta gtgtttgaag atgaaatcac tgcattacaa
      661 cctgaagtag ataagttaaa aactctaaat gtgaacaaaa ttattgcact gggacattcg
      721 ggttttgaaa tggataaact catcgctcag aaagtgaggg gtgtggacgt cgtggtggga
      781 ggacactcca acacatttct ttacacaggc aatccacctt ccaaagaggt gcctgctggg
      841 aagtacccat tcatagtcac ttctgatgat gggcggaagg ttcctgtagt ccaggcctat
      901 gcttttggca aatacctagg ctatctgaag atcgagtttg atgaaagagg aaacgtcatc
      961 tcttcccatg gaaatcccat tcttctaaac agcagcattc ctgaagatcc aagcataaaa
     1021 gcagacatta acaaatggag gataaaattg gataattatt ctacccagga attagggaaa
     1081 acaattgtct atctggatgg ctcctctcaa tcatgccgct ttagagaatg caacatgggc
     1141 aacctgattt gtgatgcaat gattaacaac aacctgagac acacggatga aatgttctgg
     1201 aaccacgtat ccatgtgcat tttaaatgga ggtggtatcc ggtcgcccat tgatgaacgc
     1261 aacaatggca caattacctg ggagaacctg gctgctgtat tgccctttgg aggcacattt
     1321 gacctagtcc agttaaaagg ttccaccctg aagaaggcct ttgagcatag cgtgcaccgc
     1381 tacggccagt ccactggaga gttcctgcag gtgggcggaa tccatgtggt gtatgatctt
     1441 tcccgaaaac ctggagacag agtagtcaaa ttagatgttc tttgcaccaa gtgtcgagtg
     1501 cccagttatg accctctcaa aatggacgag gtatataagg tgatcctccc aaacttcctg
     1561 gccaatggtg gagatgggtt ccagatgata aaagatgaat tattaagaca tgactctggt
     1621 gaccaagata tcaacgtggt ttctacatat atctccaaaa tgaaagtaat ttatccagca
     1681 gttgaaggtc ggatcaagtt ttccacagga agtcactgcc atggaagctt ttctttaata
     1741 tttctttcac tttgggcagt gatctttgtt ttataccaat agccaaaaat tctccttgcc
     1801 tttaatgtgt gaaactgcat tttttcaagt gagattcaaa tctgcctttt aggacctggc
     1861 tttgtgacag caaaaaccat ctttacaggc tcctagaagc tgaaggttag agcattataa
     1921 aatgaagaga cagacatgat tactcagggt cagcaaccta gtgagttaga aaaaaaatta
     1981 acatagggcc ctataaggag aaagccaact atgttaagtt tacgtgtcca aattttaatg
     2041 aaattttact aacaatttta aaccatattt ttcttcttca tatccatttc taatccatca
     2101 aacagcttat gtttacataa aattttatca ttcacaagga agttttaagc acactgtctc
     2161 atttgatatc cacaacttat ttttggtagg aaagagagat gtttttccca cctgtcagat
     2221 gaaaaaactg aagctcaaaa agggttgact tgaccataca gctaatgctg acagatccaa
     2281 gacctagacc taggtctttt gaactcaagt ccagcattct caactatatc aagttactgt
     2341 tcagaatact taatatctcc tctcttcata attatcaata gccccaagct catggatgac
     2401 aaatctctgc tttatttctt gtctctattt tttcacttta tagctcctgt tataatagca
     2461 agtttaatgg tataaacaca ggataccatc ctctcttgca acacccatgt gcctttgatg
     2521 agtcaggtag caagctgtag tagataatga gaaaggccag aggctgcaaa agacagtcaa
     2581 aggacacgag agaaaggaag gggaagaaca ggactccagg actgttttat attatagaaa
     2641 agcaagagct aaagagcatt tacacatgtt aaacagatac ttgttaagca tagtgcctga
     2701 cacacggcat tagctgttat tttatgagat tccatcagct ctgcctctgt cctctttctt
     2761 ctaacatgaa ggtatcatga gaagagaacc ttctaacata agctgtaatt ctaaacctgc
     2821 acttgtccct ctccagcaag aggctagcac tgaattcatt ctactcatac tacacaccca
     2881 gttatggaat gtccagagtt ctcgaagaaa ataaatgact ttaggaagag gtatacattt
     2941 tttaagtcgc tctgcctcca aatctgaaca gtcactgtaa atcattctta agcccagata
     3001 tgagaacttc tgctggaaag tgggaccctc tgagtgggtg gtcagaaaat acccatgctg
     3061 atgaaatgac ctatgcccaa agaacaaata cttaacgtgg gagtggaacc acatgagcct
     3121 gctcagctct gcataagtaa ttcaagaaat gggaggcttc accttaaaaa cagtgtgcaa
     3181 atggcagcta gaggttttga taggaagtat gtttgtttct tagtgtttac aaatattaag
     3241 tactcttgat acaaaatata cttttaaact tcataacctt tttataaaag ttgttgcagc
     3301 aaaataatag cctcggttct atgcatatat ggattagcta taaaaaatgt caataagatt
     3361 gtacaaggaa aattagagaa agtcacattt agggtttatt ttttacactt ggccagtaaa
     3421 atagggtaaa tcctattaga attttttaaa gaactttttt taagtttcct aaatctgtgt
     3481 gtgtattgtg aagtggtata agaaatgact ttgaaccact ttgcaattgt agattcccaa
     3541 caataaaatt gaagataagc tc
//
