LOCUS       NM_052935               1408 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens 5'-nucleotidase, cytosolic IIIB (NT5C3B), transcript
            variant 1, mRNA.
ACCESSION   NM_052935
VERSION     NM_052935.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Dijkstra,A.E., Postma,D.S., van Ginneken,B., Wielputz,M.O.,
            Schmidt,M., Becker,N., Owsijewitsch,M., Kauczor,H.U., de
            Koning,H.J., Lammers,J.W., Oudkerk,M., Brandsma,C.A., Bosse,Y.,
            Nickle,D.C., Sin,D.D., Hiemstra,P.S., Wijmenga,C., Smolonska,J.,
            Zanen,P., Vonk,J.M., van den Berge,M., Boezen,H.M. and Groen,H.J.
  TITLE     Novel genes for airway wall thickness identified with combined
            genome-wide association and expression analyses
  JOURNAL   Am J Respir Crit Care Med 191 (5), 547-556 (2015)
   PUBMED   25517131
  REMARK    GeneRIF: NT5C3B is involved in airway wall thickening and thereby
            with airway obstruction.
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Monecke,T., Buschmann,J., Neumann,P., Wahle,E. and Ficner,R.
  TITLE     Crystal structures of the novel cytosolic 5'-nucleotidase IIIB
            explain its preference for m7GMP
  JOURNAL   PLoS One 9 (3), e90915 (2014)
   PUBMED   24603684
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1408)
  AUTHORS   Buschmann,J., Moritz,B., Jeske,M., Lilie,H., Schierhorn,A. and
            Wahle,E.
  TITLE     Identification of Drosophila and human 7-methyl GMP-specific
            nucleotidases
  JOURNAL   J Biol Chem 288 (4), 2441-2451 (2013)
   PUBMED   23223233
REFERENCE   4  (bases 1 to 1408)
  AUTHORS   Brieger,A., Adryan,B., Wolpert,F., Passmann,S., Zeuzem,S. and
            Trojan,J.
  TITLE     Cytoskeletal scaffolding proteins interact with Lynch-Syndrome
            associated mismatch repair protein MLH1
  JOURNAL   Proteomics 10 (18), 3343-3355 (2010)
   PUBMED   20706999
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CN393115.1, DR159341.1, BF339623.1, AC091172.11 and R61279.1.
            
            On May 17, 2019 this sequence version replaced NM_052935.4.
            
            Transcript Variant: This variant (1) represents the shortest
            transcript but encodes the functional protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7410570.632371.1,
                                           SRR7410570.632370.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467144, SAMEA2467146
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000435506.7/ ENSP00000389948.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-365               CN393115.1         37-401
            366-546             DR159341.1         580-760
            547-915             BF339623.1         47-415
            916-1337            AC091172.11        42362-42783         c
            1338-1383           R61279.1           9-54                c
            1384-1408           AC091172.11        42291-42315         c
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.2"
     gene            1..1408
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /note="5'-nucleotidase, cytosolic IIIB"
                     /db_xref="GeneID:115024"
                     /db_xref="HGNC:HGNC:28300"
                     /db_xref="MIM:620041"
     exon            1..51
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..942
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.91"
                     /note="cytosolic 5'-nucleotidase III-like protein;
                     cN-III-like protein; 5'-nucleotidase, cytosolic III-like;
                     cytosolic 5'-nucleotidase 3B; 7-methylguanosine
                     nucleotidase; N(7)-methylguanylate 5'-phosphatase"
                     /codon_start=1
                     /product="7-methylguanosine phosphate-specific
                     5'-nucleotidase"
                     /protein_id="NP_443167.4"
                     /db_xref="CCDS:CCDS11410.2"
                     /db_xref="GeneID:115024"
                     /db_xref="HGNC:HGNC:28300"
                     /db_xref="MIM:620041"
                     /translation="MAEEVSTLMKATVLMRQPGRVQEIVGALRKGGGDRLQVISDFDM
                     TLSRFAYNGKRCPSSYNILDNSKIISEECRKELTALLHHYYPIEIDPHRTVKEKLPHM
                     VEWWTKAHNLLCQQKIQKFQIAQVVRESNAMLREGYKTFFNTLYHNNIPLFIFSAGIG
                     DILEEIIRQMKVFHPNIHIVSNYMDFNEDGFLQGFKGQLIHTYNKNSSACENSGYFQQ
                     LEGKTNVILLGDSIGDLTMADGVPGVQNILKIGFLNDKVEERRERYMDSYDIVLEKDE
                     TLDVVNGLLQHILCQGVQLEMQGP"
     exon            52..150
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /inference="alignment:Splign:2.1.0"
     exon            151..220
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /inference="alignment:Splign:2.1.0"
     exon            221..267
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /inference="alignment:Splign:2.1.0"
     exon            268..353
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /inference="alignment:Splign:2.1.0"
     exon            354..443
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /inference="alignment:Splign:2.1.0"
     exon            444..606
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /inference="alignment:Splign:2.1.0"
     exon            607..807
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /inference="alignment:Splign:2.1.0"
     exon            808..1408
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1240..1245
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /note="hexamer: AATATA"
     polyA_site      1241
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
                     /note="major polyA site"
     polyA_site      1265
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
     polyA_site      1387
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
     polyA_site      1408
                     /gene="NT5C3B"
                     /gene_synonym="cN-IIIB; CNIIIL; NT5C3L"
ORIGIN      
        1 gtcgttgtcc cgcgagccgc cgaccaggcc tcgaacggga tggcagagga ggtgagcacc
       61 ctgatgaagg ccacggtcct gatgcggcag cctgggcggg tgcaggagat cgtgggcgcc
      121 ctccgcaagg gcggcggaga ccggttacag gtgatttctg attttgacat gaccttgagc
      181 aggtttgcat ataatggaaa gcgatgccct tcttcttaca atattctgga taatagcaag
      241 atcatcagtg aggagtgtcg gaaagagctc acagcgctcc ttcaccacta ttacccaatt
      301 gagatcgacc cacaccggac cgtcaaggag aagctacctc atatggtgga atggtggacc
      361 aaagcacaca atctcctatg tcagcagaag attcagaagt ttcagatagc ccaggtggtt
      421 agagagtcca atgcaatgct cagggaggga tataagacct tcttcaacac actctaccat
      481 aacaacattc cccttttcat cttttctgcg ggcattggtg atatcctgga agaaattatc
      541 cgacagatga aagtgttcca ccccaacatc cacatcgtgt ctaactacat ggattttaat
      601 gaagatggtt ttctccaggg atttaagggc cagctcatac acacatacaa caagaacagc
      661 tctgcgtgtg agaactctgg ttacttccag caacttgagg gcaaaaccaa tgtcatcctg
      721 ctgggagact ctatcgggga cctcaccatg gccgatgggg ttcctggtgt gcagaacatt
      781 ctcaaaattg gcttcctgaa tgacaaggtg gaggagcggc gggagcgcta catggactcc
      841 tatgacatcg tgctggagaa ggacgagact ctggatgtgg tcaacgggct actgcagcac
      901 atcctgtgcc agggggtcca gctggagatg caaggcccct gaaggcgcag gctccagccc
      961 ggcctgcagg ccgtggtgag gaggggcgcc tccccagagt ctgctccccc gtgaacacag
     1021 agcagaggcc agggtggcca gcagtggctg ggtccttccg cgcccctccg tcctcctttc
     1081 cctgagcacc ttcatcacca gaggcttgaa ggaaccccgc catgtggcag ggcacaggca
     1141 ctgttcctgg tgaaccttgg accacagcat gtcagtgctc tagggattgt ctactccagg
     1201 gattttcttc aaaattttta aacatgggaa gttcaaacaa atataatgtg tgaaacagat
     1261 caaaattttt aaaatgaaaa aaaagctgct ctgattcagg ggatgtgggt gggggtagaa
     1321 cctggacctc ttggccctgg gggcacatgg gatgcttcta ggaacacagt ttgagaacca
     1381 ccacaaattg gcctgagacc ccatccga
//
