LOCUS       NM_001351170            3405 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens 5'-nucleotidase, cytosolic II (NT5C2), transcript
            variant 4, mRNA.
ACCESSION   NM_001351170 XM_005269632
VERSION     NM_001351170.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Aabdien,A., Sichlinger,L., Borgel,Z., Jones,M.R., Waston,I.A.,
            Gatford,N.J.F., Raval,P., Tanangonan,L., Powell,T.R., Duarte,R.R.R.
            and Srivastava,D.P.
  TITLE     Schizophrenia risk proteins ZNF804A and NT5C2 interact in cortical
            neurons
  JOURNAL   Eur J Neurosci 59 (8), 2102-2117 (2024)
   PUBMED   38279611
  REMARK    GeneRIF: Schizophrenia risk proteins ZNF804A and NT5C2 interact in
            cortical neurons.
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Zhang,W., Wang,Y.D., Xing,Y.J., Liu,P.J. and Yang,J.H.
  TITLE     Silencing of circ-NT5C2 retards the progression of IL-1beta-induced
            osteoarthritis in an in vitro cell model by targeting the
            miR-142-5p/NAMPT axis
  JOURNAL   Microbiol Immunol 67 (3), 129-141 (2023)
   PUBMED   36540014
  REMARK    GeneRIF: Silencing of circ-NT5C2 retards the progression of
            IL-1beta-induced osteoarthritis in an in vitro cell model by
            targeting the miR-142-5p/NAMPT axis.
REFERENCE   3  (bases 1 to 3405)
  AUTHORS   Fei,X., Wu,X., Dou,Y.N., Sun,K., Guo,Q., Zhang,L., Li,S., Wei,J.,
            Huan,Y., He,X. and Fei,Z.
  TITLE     TRIM22 orchestrates the proliferation of GBMs and the benefits of
            TMZ by coordinating the modification and degradation of RIG-I
  JOURNAL   Mol Ther Oncolytics 26, 413-428 (2022)
   PUBMED   36159777
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3405)
  AUTHORS   Mei,H., Yin,B., Yang,W., Zhang,J., Lu,H., Qi,X., Mei,W., Zhang,H.
            and Zhang,J.
  TITLE     Associations between Gene-Gene Interaction and Overweight/Obesity
            of 12-Month-Old Chinese Infants
  JOURNAL   Biomed Res Int 2022, 1499454 (2022)
   PUBMED   35295960
  REMARK    GeneRIF: Associations between Gene-Gene Interaction and
            Overweight/Obesity of 12-Month-Old Chinese Infants.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3405)
  AUTHORS   Wallden,K., Stenmark,P., Nyman,T., Flodin,S., Graslund,S.,
            Loppnau,P., Bianchi,V. and Nordlund,P.
  TITLE     Crystal structure of human cytosolic 5'-nucleotidase II: insights
            into allosteric regulation and substrate recognition
  JOURNAL   J Biol Chem 282 (24), 17828-17836 (2007)
   PUBMED   17405878
  REMARK    GeneRIF: Data describe the crystal structure of human cytosolic
            5'-nucleotidase II and discuss its allosteric regulation and
            substrate recognition.
REFERENCE   6  (bases 1 to 3405)
  AUTHORS   Oka,J., Matsumoto,A., Hosokawa,Y. and Inoue,S.
  TITLE     Molecular cloning of human cytosolic purine 5'-nucleotidase
  JOURNAL   Biochem Biophys Res Commun 205 (1), 917-922 (1994)
   PUBMED   7999131
REFERENCE   7  (bases 1 to 3405)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
REFERENCE   8  (bases 1 to 3405)
  AUTHORS   Tozzi,M.G., Camici,M., Pesi,R., Allegrini,S., Sgarrella,F. and
            Ipata,P.L.
  TITLE     Nucleoside phosphotransferase activity of human colon carcinoma
            cytosolic 5'-nucleotidase
  JOURNAL   Arch Biochem Biophys 291 (2), 212-217 (1991)
   PUBMED   1659319
REFERENCE   9  (bases 1 to 3405)
  AUTHORS   Spychala,J., Madrid-Marina,V. and Fox,I.H.
  TITLE     High Km soluble 5'-nucleotidase from human placenta. Properties and
            allosteric regulation by IMP and ATP
  JOURNAL   J Biol Chem 263 (35), 18759-18765 (1988)
   PUBMED   2848805
REFERENCE   10 (bases 1 to 3405)
  AUTHORS   Yokota,S., Oka,J., Ozasa,H. and Itoh,R.
  TITLE     Immunocytochemical localization of cytosol 5'-nucleotidase in
            chicken liver
  JOURNAL   J Histochem Cytochem 36 (8), 983-989 (1988)
   PUBMED   2839573
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB169780.1, DB077941.1,
            AK291667.1, BC001595.1 and BU618027.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001351170.1.
            
            Summary: This gene encodes a hydrolase that serves as an important
            role in cellular purine metabolism by acting primarily on inosine
            5'-monophosphate and other purine nucleotides. [provided by RefSeq,
            Oct 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.82425.1,
                                           SRR11853563.4301.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                DB169780.1         37-59
            24-548              DB077941.1         51-575
            549-1534            AK291667.1         525-1510
            1535-3288           BC001595.1         1594-3347
            3289-3405           BU618027.1         39-155              c
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.32-q24.33"
     gene            1..3405
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /note="5'-nucleotidase, cytosolic II"
                     /db_xref="GeneID:22978"
                     /db_xref="HGNC:HGNC:8022"
                     /db_xref="MIM:600417"
     exon            1..37
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            38..162
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..1771
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.99"
                     /EC_number="2.7.1.77"
                     /note="isoform 2 is encoded by transcript variant 4;
                     5'-nucleotidase (purine), cytosolic type B; IMP-specific
                     5'-NT; high Km 5'-nucleotidase; spastic paraplegia 45
                     (autosomal recessive); epididymis secretory sperm binding
                     protein; cytosolic IMP/GMP-specific 5'-nucleotidase;
                     cytosolic nucleoside phosphotransferase 5'N"
                     /codon_start=1
                     /product="cytosolic purine 5'-nucleotidase isoform 2"
                     /protein_id="NP_001338099.1"
                     /db_xref="CCDS:CCDS91340.1"
                     /db_xref="GeneID:22978"
                     /db_xref="HGNC:HGNC:8022"
                     /db_xref="MIM:600417"
                     /translation="MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAME
                     KIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVF
                     DTLYGNLLKVDAYGNLLVCAHGFNFIRGSQVAVQKRPETREQYPNKFIQRDDTERFYI
                     LNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHY
                     KGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPH
                     GPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIV
                     YSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTD
                     KSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFR
                     SGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEM
                     ESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEE
                     EEE"
     exon            163..236
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            237..354
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            355..450
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            451..474
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            475..566
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            567..624
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            625..718
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            719..772
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            773..856
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            857..898
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1006
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1073
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1244
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1296
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1357
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1534
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..3405
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3268..3273
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /note="hexamer: AATAAA"
     polyA_site      3288
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /note="major polyA site"
     regulatory      3386..3391
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /note="hexamer: AATAAA"
     polyA_site      3405
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
ORIGIN      
        1 agttgcgcgc tgggattgtt gcggtgcgct ggagccgaat acaaaataca gttaaaataa
       61 aatgtcaacc tcctggagtg atcggttaca gaatgcagca gatatgcctg ctaacatgga
      121 taagcatgcc ctgaaaaagt atcgtcgaga agcctatcat cgggtgtttg tgaaccgaag
      181 tttagcaatg gaaaagataa agtgttttgg ttttgatatg gattataccc ttgctgtgta
      241 caagtcccca gagtatgagt cccttggttt tgagcttact gtggagagat tagtttctat
      301 tggctatccc caggagttgc tcagctttgc ttatgattct acattcccta ccaggggact
      361 tgtctttgac acactgtatg gaaatctttt gaaagtcgat gcctatggaa acctcttggt
      421 ctgtgcacat ggatttaact ttataagggg ttctcaggta gctgttcaga agagaccaga
      481 aactagagaa cagtatccaa ataaatttat ccagcgagat gatactgaaa gattttacat
      541 tctgaacaca ctattcaacc taccagagac ctacctgttg gcctgcctag tagatttttt
      601 tactaattgt cccagatata ccagttgtga aacaggattt aaagatgggg acctcttcat
      661 gtcctaccgg agtatgttcc aggatgtaag agatgctgtt gactgggttc attacaaggg
      721 ctcccttaag gaaaagacag ttgaaaatct tgagaagtat gtagtcaaag atggaaaact
      781 gcctttgctt ctgagccgga tgaaggaagt agggaaagta tttcttgcta ccaacagtga
      841 ctataaatat acagataaaa ttatgactta cctgtttgac ttcccacatg gccccaagcc
      901 tgggagctcc catcgaccat ggcagtccta ctttgacttg atcttggtgg atgcacggaa
      961 accactcttt tttggagaag gcacagtact gcgtcaggtg gatactaaaa ctggcaagct
     1021 gaaaattggt acctacacag ggcccctaca gcatggtatc gtctactcag gaggttcttc
     1081 tgatacgatc tgtgacctgt tgggagccaa gggaaaagac attttgtata ttggagatca
     1141 catttttggg gacattttaa aatcaaagaa acggcaaggg tggcgaactt ttttggtgat
     1201 tcctgaactc gcacaggagc tacatgtctg gactgacaag agttcacttt tcgaagaact
     1261 tcagagcttg gatattttct tggctgaact ctacaagcat cttgacagca gtagcaatga
     1321 gcgtccagac atcagttcca tccagagacg tattaagaaa gtaactcatg acatggacat
     1381 gtgctatggg atgatgggaa gcctgtttcg cagtggctcc cggcagaccc tttttgccag
     1441 tcaagtgatg cgttatgctg acctctatgc agcatctttc atcaacctgc tgtattaccc
     1501 tttcagctac ctcttcaggg ctgcccatgt cttgatgcct catgaatcaa cggtggagca
     1561 cacacacgta gatatcaatg agatggagtc tcctcttgcc acccggaacc gcacatcagt
     1621 ggatttcaaa gacactgact acaagcggca ccagctgaca cggtcaatta gtgagattaa
     1681 acctcccaac ctcttcccac tggcccccca ggaaattaca cactgccatg acgaagatga
     1741 tgatgaagag gaggaggagg aggaagaata aggaggaaaa ccaaaacccc aagcacccat
     1801 taaacaagtc ctggcaggac tcacaggaac aaacgaggtc cctgttaggg ttctactcgg
     1861 gggagggagg gggctccatg aaaggtacgt ctgaaaagtt tctgaagatt ttattatcat
     1921 agatacttgt tttggttttg tgtatctgta ctctctgcag atggtccaaa attgtaatgg
     1981 agtctgtatt agaagaaaat aagggtaaaa tcaggctgaa ctgcatgtat atggctccac
     2041 tgtggcttgt gacactttta aaatcatccg tatgtcagtg tatctggata cacgaggaaa
     2101 aggaaagagt ctcagagtgg aacaaagagt gggaagaggt gatctgtaat gttacaaatt
     2161 gtgctattac tccaaggtcc aacttttcca gtgcattaca tggtattgta tatcagtgga
     2221 gaaatgtatt atttccatga tcaaatgtag tctctgttaa ggtcaagttt tcttttataa
     2281 gcctttaatt catcctcagt gactctggca aggctgcttc tctatcactg gctttgcaca
     2341 gaagtatgct ctacttgcgt tgctttaggg caggattcta ttttgaggga aaagacagta
     2401 tccttattac cttttgtttg tttaatagca caaatgctta tttgttatcc aaaaacaacc
     2461 tccttcttat ctgtgataaa tctatagaaa gaatttagct gcaagtggac aaaggaacaa
     2521 gcccccagaa aagaaaggga agaactgcct tcttatacta cagaacatgc attagtgtgg
     2581 gctatatagc tgtggctcat gctacccaat tccagatttc tttgtcctct aagagttgat
     2641 tgctgtatat taaaattgaa catcagagga tgggaagagg gctctgtaag ccagaacctt
     2701 actaaagtag agggcacaat cagtgtgaat aaattcactt cagaatctca agtcaaggcc
     2761 aggcacggcg gctcacgcct gtaatcccag cactttggga ggccgagaca ggcggatcac
     2821 ctgaggtcgg gagttcgaga ccagccttac caacatggag aaaccccatc tctactaaaa
     2881 atacaaaatt acctgggcgt ggtggtgcat gcctgtaatc ccatcatcta ctcaggaggc
     2941 tgaggcagga gaattgcttg aacccaggag gcggaggttg cagtgagcca ggattgtgcc
     3001 attgcactcc agcctgggca acaagaacaa aactccatct caaaaaataa aaatcccaat
     3061 cccaagtcga aatcacctct tgttttaaac aagaatgaat cattactgtg tatgttaggg
     3121 tattaaaact gtttcaccag tacagtgaaa gttgtttcaa cattttaaac aaacagtggt
     3181 tatagactct ttctttaacc attgtatatt ttcttccatt cttgtcattg gtcaataggg
     3241 gagggtagat tagctgctcc agaattcaat aaagtgtaat atttctaacg gtgactttga
     3301 cctattctgt agtacaactg taatagctat tggtcttcaa gtgggtttag atttggtgac
     3361 atcagtttga tattctctta aaggaaataa atattcaaga actga
//
