LOCUS       NM_001351169            3525 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens 5'-nucleotidase, cytosolic II (NT5C2), transcript
            variant 3, mRNA.
ACCESSION   NM_001351169 XM_005269636
VERSION     NM_001351169.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Aabdien,A., Sichlinger,L., Borgel,Z., Jones,M.R., Waston,I.A.,
            Gatford,N.J.F., Raval,P., Tanangonan,L., Powell,T.R., Duarte,R.R.R.
            and Srivastava,D.P.
  TITLE     Schizophrenia risk proteins ZNF804A and NT5C2 interact in cortical
            neurons
  JOURNAL   Eur J Neurosci 59 (8), 2102-2117 (2024)
   PUBMED   38279611
  REMARK    GeneRIF: Schizophrenia risk proteins ZNF804A and NT5C2 interact in
            cortical neurons.
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Zhang,W., Wang,Y.D., Xing,Y.J., Liu,P.J. and Yang,J.H.
  TITLE     Silencing of circ-NT5C2 retards the progression of IL-1beta-induced
            osteoarthritis in an in vitro cell model by targeting the
            miR-142-5p/NAMPT axis
  JOURNAL   Microbiol Immunol 67 (3), 129-141 (2023)
   PUBMED   36540014
  REMARK    GeneRIF: Silencing of circ-NT5C2 retards the progression of
            IL-1beta-induced osteoarthritis in an in vitro cell model by
            targeting the miR-142-5p/NAMPT axis.
REFERENCE   3  (bases 1 to 3525)
  AUTHORS   Fei,X., Wu,X., Dou,Y.N., Sun,K., Guo,Q., Zhang,L., Li,S., Wei,J.,
            Huan,Y., He,X. and Fei,Z.
  TITLE     TRIM22 orchestrates the proliferation of GBMs and the benefits of
            TMZ by coordinating the modification and degradation of RIG-I
  JOURNAL   Mol Ther Oncolytics 26, 413-428 (2022)
   PUBMED   36159777
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3525)
  AUTHORS   Mei,H., Yin,B., Yang,W., Zhang,J., Lu,H., Qi,X., Mei,W., Zhang,H.
            and Zhang,J.
  TITLE     Associations between Gene-Gene Interaction and Overweight/Obesity
            of 12-Month-Old Chinese Infants
  JOURNAL   Biomed Res Int 2022, 1499454 (2022)
   PUBMED   35295960
  REMARK    GeneRIF: Associations between Gene-Gene Interaction and
            Overweight/Obesity of 12-Month-Old Chinese Infants.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3525)
  AUTHORS   Wallden,K., Stenmark,P., Nyman,T., Flodin,S., Graslund,S.,
            Loppnau,P., Bianchi,V. and Nordlund,P.
  TITLE     Crystal structure of human cytosolic 5'-nucleotidase II: insights
            into allosteric regulation and substrate recognition
  JOURNAL   J Biol Chem 282 (24), 17828-17836 (2007)
   PUBMED   17405878
  REMARK    GeneRIF: Data describe the crystal structure of human cytosolic
            5'-nucleotidase II and discuss its allosteric regulation and
            substrate recognition.
REFERENCE   6  (bases 1 to 3525)
  AUTHORS   Oka,J., Matsumoto,A., Hosokawa,Y. and Inoue,S.
  TITLE     Molecular cloning of human cytosolic purine 5'-nucleotidase
  JOURNAL   Biochem Biophys Res Commun 205 (1), 917-922 (1994)
   PUBMED   7999131
REFERENCE   7  (bases 1 to 3525)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
REFERENCE   8  (bases 1 to 3525)
  AUTHORS   Tozzi,M.G., Camici,M., Pesi,R., Allegrini,S., Sgarrella,F. and
            Ipata,P.L.
  TITLE     Nucleoside phosphotransferase activity of human colon carcinoma
            cytosolic 5'-nucleotidase
  JOURNAL   Arch Biochem Biophys 291 (2), 212-217 (1991)
   PUBMED   1659319
REFERENCE   9  (bases 1 to 3525)
  AUTHORS   Spychala,J., Madrid-Marina,V. and Fox,I.H.
  TITLE     High Km soluble 5'-nucleotidase from human placenta. Properties and
            allosteric regulation by IMP and ATP
  JOURNAL   J Biol Chem 263 (35), 18759-18765 (1988)
   PUBMED   2848805
REFERENCE   10 (bases 1 to 3525)
  AUTHORS   Yokota,S., Oka,J., Ozasa,H. and Itoh,R.
  TITLE     Immunocytochemical localization of cytosol 5'-nucleotidase in
            chicken liver
  JOURNAL   J Histochem Cytochem 36 (8), 983-989 (1988)
   PUBMED   2839573
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL360001.33 and AL139817.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 25, 2019 this sequence version replaced NM_001351169.1.
            
            Summary: This gene encodes a hydrolase that serves as an important
            role in cellular purine metabolism by acting primarily on inosine
            5'-monophosphate and other purine nucleotides. [provided by RefSeq,
            Oct 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853561.4088.1,
                                           SRR11853560.4115.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000404739.8/ ENSP00000383960.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AL360001.33        86058-86094         c
            38-181              AL360001.33        74007-74150         c
            182-306             AL360001.33        67680-67804         c
            307-380             AL360001.33        32228-32301         c
            381-498             AL139817.9         44833-44950         c
            499-594             AL139817.9         43950-44045         c
            595-686             AL139817.9         39479-39570         c
            687-744             AL139817.9         39289-39346         c
            745-838             AL139817.9         38170-38263         c
            839-892             AL139817.9         37175-37228         c
            893-976             AL139817.9         35535-35618         c
            977-1018            AL139817.9         34183-34224         c
            1019-1126           AL139817.9         32592-32699         c
            1127-1193           AL139817.9         32216-32282         c
            1194-1364           AL139817.9         31383-31553         c
            1365-1416           AL139817.9         29808-29859         c
            1417-1477           AL139817.9         29180-29240         c
            1478-1654           AL139817.9         28855-29031         c
            1655-3525           AL139817.9         26282-28152         c
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.32-q24.33"
     gene            1..3525
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /note="5'-nucleotidase, cytosolic II"
                     /db_xref="GeneID:22978"
                     /db_xref="HGNC:HGNC:8022"
                     /db_xref="MIM:600417"
     exon            1..37
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            38..181
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            182..306
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     CDS             206..1891
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.99"
                     /EC_number="2.7.1.77"
                     /note="isoform 1 is encoded by transcript variant 3;
                     5'-nucleotidase (purine), cytosolic type B; IMP-specific
                     5'-NT; high Km 5'-nucleotidase; spastic paraplegia 45
                     (autosomal recessive); epididymis secretory sperm binding
                     protein; cytosolic IMP/GMP-specific 5'-nucleotidase;
                     cytosolic nucleoside phosphotransferase 5'N"
                     /codon_start=1
                     /product="cytosolic purine 5'-nucleotidase isoform 1"
                     /protein_id="NP_001338098.1"
                     /db_xref="CCDS:CCDS7544.1"
                     /db_xref="GeneID:22978"
                     /db_xref="HGNC:HGNC:8022"
                     /db_xref="MIM:600417"
                     /translation="MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAME
                     KIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVF
                     DTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLP
                     ETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKT
                     VENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSH
                     RPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDT
                     ICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEEL
                     QSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLF
                     ASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRN
                     RTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEEE"
     exon            307..380
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            381..498
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            499..594
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            595..686
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            687..744
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            745..838
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            839..892
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            893..976
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1018
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1126
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1193
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1364
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1416
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1477
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1654
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..3525
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3388..3393
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /note="hexamer: AATAAA"
     polyA_site      3408
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /note="major polyA site"
     regulatory      3506..3511
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
                     /note="hexamer: AATAAA"
     polyA_site      3525
                     /gene="NT5C2"
                     /gene_synonym="cN-II; GMP; NT5B; PNT5; SPG45; SPG65"
ORIGIN      
        1 agttgcgcgc tgggattgtt gcggtgcgct ggagccgagt ttcgctctgc tgcccaggct
       61 ggagtacagt gatgtgatct cctgacgtca agtgatccat tcgcctcggc ctcccaaagt
      121 tctgggatta caagtgtgag ccactgccac tggctgaaaa agtcttattg tggcataatt
      181 gaatacaaaa tacagttaaa ataaaatgtc aacctcctgg agtgatcggt tacagaatgc
      241 agcagatatg cctgctaaca tggataagca tgccctgaaa aagtatcgtc gagaagccta
      301 tcatcgggtg tttgtgaacc gaagtttagc aatggaaaag ataaagtgtt ttggttttga
      361 tatggattat acccttgctg tgtacaagtc cccagagtat gagtcccttg gttttgagct
      421 tactgtggag agattagttt ctattggcta tccccaggag ttgctcagct ttgcttatga
      481 ttctacattc cctaccaggg gacttgtctt tgacacactg tatggaaatc ttttgaaagt
      541 cgatgcctat ggaaacctct tggtctgtgc acatggattt aactttataa ggggaccaga
      601 aactagagaa cagtatccaa ataaatttat ccagcgagat gatactgaaa gattttacat
      661 tctgaacaca ctattcaacc taccagagac ctacctgttg gcctgcctag tagatttttt
      721 tactaattgt cccagatata ccagttgtga aacaggattt aaagatgggg acctcttcat
      781 gtcctaccgg agtatgttcc aggatgtaag agatgctgtt gactgggttc attacaaggg
      841 ctcccttaag gaaaagacag ttgaaaatct tgagaagtat gtagtcaaag atggaaaact
      901 gcctttgctt ctgagccgga tgaaggaagt agggaaagta tttcttgcta ccaacagtga
      961 ctataaatat acagataaaa ttatgactta cctgtttgac ttcccacatg gccccaagcc
     1021 tgggagctcc catcgaccat ggcagtccta ctttgacttg atcttggtgg atgcacggaa
     1081 accactcttt tttggagaag gcacagtact gcgtcaggtg gatactaaaa ctggcaagct
     1141 gaaaattggt acctacacag ggcccctaca gcatggtatc gtctactcag gaggttcttc
     1201 tgatacgatc tgtgacctgt tgggagccaa gggaaaagac attttgtata ttggagatca
     1261 catttttggg gacattttaa aatcaaagaa acggcaaggg tggcgaactt ttttggtgat
     1321 tcctgaactc gcacaggagc tacatgtctg gactgacaag agttcacttt tcgaagaact
     1381 tcagagcttg gatattttct tggctgaact ctacaagcat cttgacagca gtagcaatga
     1441 gcgtccagac atcagttcca tccagagacg tattaagaaa gtaactcatg acatggacat
     1501 gtgctatggg atgatgggaa gcctgtttcg cagtggctcc cggcagaccc tttttgccag
     1561 tcaagtgatg cgttatgctg acctctatgc agcatctttc atcaacctgc tgtattaccc
     1621 tttcagctac ctcttcaggg ctgcccatgt cttgatgcct catgaatcaa cggtggagca
     1681 cacacacgta gatatcaatg agatggagtc tcctcttgcc acccggaacc gcacatcagt
     1741 ggatttcaaa gacactgact acaagcggca ccagctgaca cggtcaatta gtgagattaa
     1801 acctcccaac ctcttcccac tggcccccca ggaaattaca cactgccatg acgaagatga
     1861 tgatgaagag gaggaggagg aggaagaata aggaggaaaa ccaaaacccc aagcacccat
     1921 taaacaagtc ctggcaggac tcacaggaac aaacgaggtc cctgttaggg ttctactcgg
     1981 gggagggagg gggctccatg aaaggtacgt ctgaaaagtt tctgaagatt ttattatcat
     2041 agatacttgt tttggttttg tgtatctgta ctctctgcag atggtccaaa attgtaatgg
     2101 agtctgtatt agaagaaaat aagggtaaaa tcaggctgaa ctgcatgtat atggctccac
     2161 tgtggcttgt gacactttta aaatcatccg tatgtcagtg tatctggata cacgaggaaa
     2221 aggaaagagt ctcagagtgg aacaaagagt gggaagaggt gatctgtaat gttacaaatt
     2281 gtgctattac tccaaggtcc aacttttcca gtgcattaca tggtattgta tatcagtgga
     2341 gaaatgtatt atttccatga tcaaatgtag tctctgttaa ggtcaagttt tcttttataa
     2401 gcctttaatt catcctcagt gactctggca aggctgcttc tctatcactg gctttgcaca
     2461 gaagtatgct ctacttgcgt tgctttaggg caggattcta ttttgaggga aaagacagta
     2521 tccttattac cttttgtttg tttaatagca caaatgctta tttgttatcc aaaaacaacc
     2581 tccttcttat ctgtgataaa tctatagaaa gaatttagct gcaagtggac aaaggaacaa
     2641 gcccccagaa aagaaaggga agaactgcct tcttatacta cagaacatgc attagtgtgg
     2701 gctatatagc tgtggctcat gctacccaat tccagatttc tttgtcctct aagagttgat
     2761 tgctgtatat taaaattgaa catcagagga tgggaagagg gctctgtaag ccagaacctt
     2821 actaaagtag agggcacaat cagtgtgaat aaattcactt cagaatctca agtcaaggcc
     2881 aggcacggcg gctcacgcct gtaatcccag cactttggga ggccgagaca ggcggatcac
     2941 ctgaggtcgg gagttcgaga ccagccttac caacatggag aaaccccatc tctactaaaa
     3001 atacaaaatt acctgggcgt ggtggtgcat gcctgtaatc ccatcatcta ctcaggaggc
     3061 tgaggcagga gaattgcttg aacccaggag gcggaggttg cagtgagcca ggattgtgcc
     3121 attgcactcc agcctgggca acaagaacaa aactccatct caaaaaataa aaatcccaat
     3181 cccaagtcga aatcacctct tgttttaaac aagaatgaat cattactgtg tatgttaggg
     3241 tattaaaact gtttcaccag tacagtgaaa gttgtttcaa cattttaaac aaacagtggt
     3301 tatagactct ttctttaacc attgtatatt ttcttccatt cttgtcattg gtcaataggg
     3361 gagggtagat tagctgctcc agaattcaat aaagtgtaat atttctaacg gtgactttga
     3421 cctattctgt agtacaactg taatagctat tggtcttcaa gtgggtttag atttggtgac
     3481 atcagtttga tattctctta aaggaaataa atattcaaga actga
//
