LOCUS       NM_182543               2166 bp    mRNA    linear   PRI 12-APR-2024
DEFINITION  Homo sapiens NOP2/Sun RNA methyltransferase 6 (NSUN6), transcript
            variant 1, mRNA.
ACCESSION   NM_182543
VERSION     NM_182543.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Lu,Z., Liu,B., Kong,D., Zhou,X., Pei,D. and Liu,D.
  TITLE     NSUN6 Regulates NM23-H1 Expression in an m5C Manner to Affect
            Epithelial-Mesenchymal Transition in Lung Cancer
  JOURNAL   Med Princ Pract 33 (1), 56-65 (2024)
   PUBMED   38029727
  REMARK    GeneRIF: NSUN6 Regulates NM23-H1 Expression in an m5C Manner to
            Affect Epithelial-Mesenchymal Transition in Lung Cancer.
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Mattioli,F., Worpenberg,L., Li,C.T., Ibrahim,N., Naz,S., Sharif,S.,
            Firouzabadi,S.G., Vosoogh,S., Saraeva-Lamri,R., Raymond,L.,
            Trujillo,C., Guex,N., Antonarakis,S.E., Ansar,M., Darvish,H.,
            Liu,R.J., Roignant,J.Y. and Reymond,A.
  TITLE     Biallelic variants in NSUN6 cause an autosomal recessive
            neurodevelopmental disorder
  JOURNAL   Genet Med 25 (9), 100900 (2023)
   PUBMED   37226891
  REMARK    GeneRIF: Biallelic variants in NSUN6 cause an autosomal recessive
            neurodevelopmental disorder.
REFERENCE   3  (bases 1 to 2166)
  AUTHORS   Awah,C.U., Winter,J., Mazdoom,C.M. and Ogunwobi,O.O.
  TITLE     NSUN6, an RNA methyltransferase of 5-mC controls glioblastoma
            response to temozolomide (TMZ) via NELFB and RPS6KB2 interaction
  JOURNAL   Cancer Biol Ther 22 (10-12), 587-597 (2021)
   PUBMED   34705606
  REMARK    GeneRIF: NSUN6, an RNA methyltransferase of 5-mC controls
            glioblastoma response to temozolomide (TMZ) via NELFB and RPS6KB2
            interaction.
REFERENCE   4  (bases 1 to 2166)
  AUTHORS   Selmi,T., Hussain,S., Dietmann,S., Heiss,M., Borland,K., Flad,S.,
            Carter,J.M., Dennison,R., Huang,Y.L., Kellner,S., Bornelov,S. and
            Frye,M.
  TITLE     Sequence- and structure-specific cytosine-5 mRNA methylation by
            NSUN6
  JOURNAL   Nucleic Acids Res 49 (2), 1006-1022 (2021)
   PUBMED   33330931
  REMARK    GeneRIF: Sequence- and structure-specific cytosine-5 mRNA
            methylation by NSUN6.
REFERENCE   5  (bases 1 to 2166)
  AUTHORS   Yang,R., Liang,X., Wang,H., Guo,M., Shen,H., Shi,Y., Liu,Q.,
            Sun,Y., Yang,L. and Zhan,M.
  TITLE     The RNA methyltransferase NSUN6 suppresses pancreatic cancer
            development by regulating cell proliferation
  JOURNAL   EBioMedicine 63, 103195 (2021)
   PUBMED   33418496
  REMARK    GeneRIF: The RNA methyltransferase NSUN6 suppresses pancreatic
            cancer development by regulating cell proliferation.
REFERENCE   6  (bases 1 to 2166)
  AUTHORS   Liu,R.J., Long,T., Li,J., Li,H. and Wang,E.D.
  TITLE     Structural basis for substrate binding and catalytic mechanism of a
            human RNA:m5C methyltransferase NSun6
  JOURNAL   Nucleic Acids Res 45 (11), 6684-6697 (2017)
   PUBMED   28531330
  REMARK    GeneRIF: The structures of NSun6, a human tRNA:m5C
            methyltransferase, in the apo form and in complex with a
            full-length tRNA substrate.
REFERENCE   7  (bases 1 to 2166)
  AUTHORS   Long,T., Li,J., Li,H., Zhou,M., Zhou,X.L., Liu,R.J. and Wang,E.D.
  TITLE     Sequence-specific and Shape-selective RNA Recognition by the Human
            RNA 5-Methylcytosine Methyltransferase NSun6
  JOURNAL   J Biol Chem 291 (46), 24293-24303 (2016)
   PUBMED   27703015
  REMARK    GeneRIF: findings suggest that human NSun6 relies on a delicate
            network for RNA recognition, which involves both the primary
            sequence and tertiary structure of tRNA substrates.
REFERENCE   8  (bases 1 to 2166)
  AUTHORS   Haag,S., Warda,A.S., Kretschmer,J., Gunnigmann,M.A., Hobartner,C.
            and Bohnsack,M.T.
  TITLE     NSUN6 is a human RNA methyltransferase that catalyzes formation of
            m5C72 in specific tRNAs
  JOURNAL   RNA 21 (9), 1532-1543 (2015)
   PUBMED   26160102
  REMARK    GeneRIF: Findings identified NSUN6 as a novel mammalian m5C tRNA
            methyltransferase and suggest that methylation of tRNAs by NSUN6
            occurs during a late step of tRNA biogenesis, after the addition of
            the CCA tail and export of the tRNA from the nucleus.
REFERENCE   9  (bases 1 to 2166)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            HY169016.1, DB012566.1 and BC035778.1.
            
            On Nov 22, 2018 this sequence version replaced NM_182543.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC035778.1, AK074323.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377304.7/ ENSP00000366519.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-331               HY169016.1         55-385
            332-360             DB012566.1         361-389
            361-1858            BC035778.1         397-1894
            1859-2166           BC035778.1         1903-2210
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p12.31"
     gene            1..2166
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="NOP2/Sun RNA methyltransferase 6"
                     /db_xref="GeneID:221078"
                     /db_xref="HGNC:HGNC:23529"
                     /db_xref="MIM:617199"
     exon            1..459
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             385..1794
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     nucleolar protein (NOL1/NOP2/sun) and PUA domains 1;
                     NOL1/NOP2/Sun domain family, member 6; putative
                     methyltransferase NSUN6; ARL5B antisense RNA 1;
                     NOL1/NOP2/Sun and PUA domain-containing protein 1;
                     NOP2/Sun RNA methyltransferase family member 6; tRNA
                     (cytosine(72)-C(5))-methyltransferase NSUN6"
                     /codon_start=1
                     /product="tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
                     isoform 1"
                     /protein_id="NP_872349.1"
                     /db_xref="CCDS:CCDS7130.1"
                     /db_xref="GeneID:221078"
                     /db_xref="HGNC:HGNC:23529"
                     /db_xref="MIM:617199"
                     /translation="MSIFPKISLRPEVENYLKEGFMNKEIVTALGKQEAERKFETLLK
                     HLSHPPSFTTVRVNTHLASVQHVKNLLLDELQKQFNGLSVPILQHPDLQDVLLIPVIG
                     PRKNIKKQQCEAIVGAQCGNAVLRGAHVYAPGIVSASQFMKAGDVISVYSDIKGKCKK
                     GAKEFDGTKVFLGNGISELSRKEIFSGLPELKGMGIRMTEPVYLSPSFDSVLPRYLFL
                     QNLPSALVSHVLNPQPGEKILDLCAAPGGKTTHIAALMHDQGEVIALDKIFNKVEKIK
                     QNALLLGLNSIRAFCFDGTKAVKLDMVEDTEGEPPFLPESFDRILLDAPCSGMGQRPN
                     MACTWSVKEVASYQPLQRKLFTAAVQLLKPEGVLVYSTCTITLAENEEQVAWALTKFP
                     CLQLQPQEPQIGGEGMRGAGLSCEQLKQLQRFDPSAVPLPDTDMDSLREARREDMLRL
                     ANKDSIGFFIAKFVKCKST"
     exon            460..615
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            616..695
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            696..805
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            806..959
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1041
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1161
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1306
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1455
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1581
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..2166
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2147..2152
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="hexamer: AATAAA"
     polyA_site      2166
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="major polyA site"
ORIGIN      
        1 gatagaaaag ataaagggac cgagctgctg tcagcctggc ttactgatct gcgtccgttt
       61 caccacggat tcagttacta agcatttttt tctttttttg gttctttgca acgtgagtgg
      121 cattggctca gtgatttcca tgagcatctc taccagaaaa cattgcctcg atgaggtgtg
      181 gtggaagccc ggcagcccct tctaatcggc taggcttgag aaagcgtgta cctctgcatt
      241 tccgaaatta actcagcgtg atcggcaaga ttttcctcag catctggtgt caagacactc
      301 gtcactatta attcggaaag aaaaaaaaaa acaaaacacc gttttccagc atttctcttg
      361 gtggagaact aaacaacagg aaaaatgtct attttcccta agatatcttt gagacctgag
      421 gttgaaaact atcttaagga aggctttatg aataaggaga ttgtgactgc tttaggtaaa
      481 caagaagcag aaaggaagtt tgaaactttg ttaaagcacc tgtcacatcc tccatcattt
      541 acaactgtca gagtgaatac acatttagcc tcagtacaac atgtgaaaaa tctgttactt
      601 gatgaacttc agaagcagtt taatggatta agtgttccta ttcttcaaca tccagacctt
      661 caagatgtgt tacttattcc tgttattgga cccagaaaga atattaaaaa acaacagtgt
      721 gaagccattg ttggagccca gtgtggcaat gcagttttaa gaggagccca tgtctatgcc
      781 ccaggaattg tgtcagcatc acaatttatg aaagctggag atgttatttc tgtatactct
      841 gatattaaag gaaaatgtaa gaaaggagcc aaagaatttg atggaacaaa agtatttctt
      901 ggaaatggga tttctgaact aagccgcaaa gaaatcttca gtggattacc tgaactgaaa
      961 ggcatgggca taagaatgac agaaccagta tatctcagcc cttcatttga cagtgtactg
     1021 ccccgttact tatttttaca aaatttgcca tctgccttag taagtcatgt actaaatcct
     1081 caacctggag agaagattct agacttgtgt gcagcacctg gagggaaaac aacacacatt
     1141 gcagcactaa tgcatgatca gggagaagtt atagcactgg ataaaatctt caacaaagta
     1201 gaaaaaatca aacagaatgc cttattgtta gggctgaatt ccatcagggc attttgtttt
     1261 gatggaacaa aggcggttaa acttgatatg gtggaggaca cagaaggaga acctccattt
     1321 ctaccagaat cctttgaccg aattcttctg gatgcaccct gtagtggaat gggacagaga
     1381 ccaaacatgg cctgtacttg gtctgtgaag gaagtggcat catatcagcc attacagcga
     1441 aaactcttca ctgcagcggt tcagctgctg aagccagagg gtgtgctggt ttatagcacg
     1501 tgcactataa cactggccga aaatgaagaa caggttgcct gggccctgac aaaatttcct
     1561 tgccttcagc ttcagcccca ggaaccgcag attggaggag aaggaatgag gggagctggg
     1621 ctctcatgtg aacagttgaa acagctgcag cgatttgatc catcggctgt gccattaccg
     1681 gacactgaca tggactctct tagagaggcc agaagagaag acatgttgcg tctggctaat
     1741 aaggactcta taggtttttt tattgcaaaa tttgtaaaat gcaaaagcac ataggagagg
     1801 gatggatgct cagaaatgaa aattccaaac atttgctgtc tgtggttttt ttttttttaa
     1861 ccaaagtgtt gtcaggccaa ctgaatgatg atgtggttgc tatggaaaca gaaaaggctg
     1921 ccagctgttt taccagggat ccagagacat agaggaagta gggggtggta tgagattata
     1981 ttttctgttt ttaaaagatt tttttttttt atgtatttag tagagtataa agaaaagcag
     2041 atgcctatag atgtctggag catattttca tttgtgatct aatgttttaa tttgtaaagt
     2101 gtacaagtca tttttaatgt taaaaattag tgaatctaac aaaaggaata aattagcaat
     2161 atttga
//
