LOCUS       NP_872349                469 aa            linear   PRI 27-APR-2025
DEFINITION  tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 isoform 1 [Homo
            sapiens].
ACCESSION   NP_872349
VERSION     NP_872349.1
DBSOURCE    REFSEQ: accession NM_182543.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 469)
  AUTHORS   Lu,Z., Liu,B., Kong,D., Zhou,X., Pei,D. and Liu,D.
  TITLE     NSUN6 Regulates NM23-H1 Expression in an m5C Manner to Affect
            Epithelial-Mesenchymal Transition in Lung Cancer
  JOURNAL   Med Princ Pract 33 (1), 56-65 (2024)
   PUBMED   38029727
  REMARK    GeneRIF: NSUN6 Regulates NM23-H1 Expression in an m5C Manner to
            Affect Epithelial-Mesenchymal Transition in Lung Cancer.
REFERENCE   2  (residues 1 to 469)
  AUTHORS   Mattioli,F., Worpenberg,L., Li,C.T., Ibrahim,N., Naz,S., Sharif,S.,
            Firouzabadi,S.G., Vosoogh,S., Saraeva-Lamri,R., Raymond,L.,
            Trujillo,C., Guex,N., Antonarakis,S.E., Ansar,M., Darvish,H.,
            Liu,R.J., Roignant,J.Y. and Reymond,A.
  TITLE     Biallelic variants in NSUN6 cause an autosomal recessive
            neurodevelopmental disorder
  JOURNAL   Genet Med 25 (9), 100900 (2023)
   PUBMED   37226891
  REMARK    GeneRIF: Biallelic variants in NSUN6 cause an autosomal recessive
            neurodevelopmental disorder.
REFERENCE   3  (residues 1 to 469)
  AUTHORS   Awah,C.U., Winter,J., Mazdoom,C.M. and Ogunwobi,O.O.
  TITLE     NSUN6, an RNA methyltransferase of 5-mC controls glioblastoma
            response to temozolomide (TMZ) via NELFB and RPS6KB2 interaction
  JOURNAL   Cancer Biol Ther 22 (10-12), 587-597 (2021)
   PUBMED   34705606
  REMARK    GeneRIF: NSUN6, an RNA methyltransferase of 5-mC controls
            glioblastoma response to temozolomide (TMZ) via NELFB and RPS6KB2
            interaction.
REFERENCE   4  (residues 1 to 469)
  AUTHORS   Selmi,T., Hussain,S., Dietmann,S., Heiss,M., Borland,K., Flad,S.,
            Carter,J.M., Dennison,R., Huang,Y.L., Kellner,S., Bornelov,S. and
            Frye,M.
  TITLE     Sequence- and structure-specific cytosine-5 mRNA methylation by
            NSUN6
  JOURNAL   Nucleic Acids Res 49 (2), 1006-1022 (2021)
   PUBMED   33330931
  REMARK    GeneRIF: Sequence- and structure-specific cytosine-5 mRNA
            methylation by NSUN6.
REFERENCE   5  (residues 1 to 469)
  AUTHORS   Yang,R., Liang,X., Wang,H., Guo,M., Shen,H., Shi,Y., Liu,Q.,
            Sun,Y., Yang,L. and Zhan,M.
  TITLE     The RNA methyltransferase NSUN6 suppresses pancreatic cancer
            development by regulating cell proliferation
  JOURNAL   EBioMedicine 63, 103195 (2021)
   PUBMED   33418496
  REMARK    GeneRIF: The RNA methyltransferase NSUN6 suppresses pancreatic
            cancer development by regulating cell proliferation.
REFERENCE   6  (residues 1 to 469)
  AUTHORS   Liu,R.J., Long,T., Li,J., Li,H. and Wang,E.D.
  TITLE     Structural basis for substrate binding and catalytic mechanism of a
            human RNA:m5C methyltransferase NSun6
  JOURNAL   Nucleic Acids Res 45 (11), 6684-6697 (2017)
   PUBMED   28531330
  REMARK    GeneRIF: The structures of NSun6, a human tRNA:m5C
            methyltransferase, in the apo form and in complex with a
            full-length tRNA substrate.
REFERENCE   7  (residues 1 to 469)
  AUTHORS   Long,T., Li,J., Li,H., Zhou,M., Zhou,X.L., Liu,R.J. and Wang,E.D.
  TITLE     Sequence-specific and Shape-selective RNA Recognition by the Human
            RNA 5-Methylcytosine Methyltransferase NSun6
  JOURNAL   J Biol Chem 291 (46), 24293-24303 (2016)
   PUBMED   27703015
  REMARK    GeneRIF: findings suggest that human NSun6 relies on a delicate
            network for RNA recognition, which involves both the primary
            sequence and tertiary structure of tRNA substrates.
REFERENCE   8  (residues 1 to 469)
  AUTHORS   Haag,S., Warda,A.S., Kretschmer,J., Gunnigmann,M.A., Hobartner,C.
            and Bohnsack,M.T.
  TITLE     NSUN6 is a human RNA methyltransferase that catalyzes formation of
            m5C72 in specific tRNAs
  JOURNAL   RNA 21 (9), 1532-1543 (2015)
   PUBMED   26160102
  REMARK    GeneRIF: Findings identified NSUN6 as a novel mammalian m5C tRNA
            methyltransferase and suggest that methylation of tRNAs by NSUN6
            occurs during a late step of tRNA biogenesis, after the addition of
            the CCA tail and export of the tRNA from the nucleus.
REFERENCE   9  (residues 1 to 469)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            HY169016.1, DB012566.1 and BC035778.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC035778.1, AK074323.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377304.7/ ENSP00000366519.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p12.31"
     Protein         1..469
                     /product="tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
                     isoform 1"
                     /note="nucleolar protein (NOL1/NOP2/sun) and PUA domains
                     1; NOL1/NOP2/Sun domain family, member 6; putative
                     methyltransferase NSUN6; ARL5B antisense RNA 1;
                     NOL1/NOP2/Sun and PUA domain-containing protein 1;
                     NOP2/Sun RNA methyltransferase family member 6; tRNA
                     (cytosine(72)-C(5))-methyltransferase NSUN6"
                     /calculated_mol_wt=51639
     Region          113..202
                     /region_name="PUA_NSun6-like"
                     /note="PUA RNA-binding domain of the SAM-dependent
                     methyltransferase NSun6 and similar proteins; cd21150"
                     /db_xref="CDD:409292"
     Site            order(116,123,125..127,129..131,193..194,198..201)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409292"
     Region          <190..465
                     /region_name="RsmB"
                     /note="16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF
                     family [Translation, ribosomal structure and biogenesis];
                     COG0144"
                     /db_xref="CDD:439914"
     Site            419
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0007744|PubMed:19608861; propagated from
                     UniProtKB/Swiss-Prot (Q8TEA1.1)"
     CDS             1..469
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /coded_by="NM_182543.5:385..1794"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS7130.1"
                     /db_xref="GeneID:221078"
                     /db_xref="HGNC:HGNC:23529"
                     /db_xref="MIM:617199"
ORIGIN      
        1 msifpkislr pevenylkeg fmnkeivtal gkqeaerkfe tllkhlshpp sfttvrvnth
       61 lasvqhvknl lldelqkqfn glsvpilqhp dlqdvllipv igprknikkq qceaivgaqc
      121 gnavlrgahv yapgivsasq fmkagdvisv ysdikgkckk gakefdgtkv flgngisels
      181 rkeifsglpe lkgmgirmte pvylspsfds vlprylflqn lpsalvshvl npqpgekild
      241 lcaapggktt hiaalmhdqg evialdkifn kvekikqnal llglnsiraf cfdgtkavkl
      301 dmvedtegep pflpesfdri lldapcsgmg qrpnmactws vkevasyqpl qrklftaavq
      361 llkpegvlvy stctitlaen eeqvawaltk fpclqlqpqe pqiggegmrg aglsceqlkq
      421 lqrfdpsavp lpdtdmdslr earredmlrl ankdsigffi akfvkckst
//
