LOCUS       NM_001351118            1930 bp    mRNA    linear   PRI 12-APR-2024
DEFINITION  Homo sapiens NOP2/Sun RNA methyltransferase 6 (NSUN6), transcript
            variant 5, mRNA.
ACCESSION   NM_001351118 XM_017015914
VERSION     NM_001351118.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Lu,Z., Liu,B., Kong,D., Zhou,X., Pei,D. and Liu,D.
  TITLE     NSUN6 Regulates NM23-H1 Expression in an m5C Manner to Affect
            Epithelial-Mesenchymal Transition in Lung Cancer
  JOURNAL   Med Princ Pract 33 (1), 56-65 (2024)
   PUBMED   38029727
  REMARK    GeneRIF: NSUN6 Regulates NM23-H1 Expression in an m5C Manner to
            Affect Epithelial-Mesenchymal Transition in Lung Cancer.
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Mattioli,F., Worpenberg,L., Li,C.T., Ibrahim,N., Naz,S., Sharif,S.,
            Firouzabadi,S.G., Vosoogh,S., Saraeva-Lamri,R., Raymond,L.,
            Trujillo,C., Guex,N., Antonarakis,S.E., Ansar,M., Darvish,H.,
            Liu,R.J., Roignant,J.Y. and Reymond,A.
  TITLE     Biallelic variants in NSUN6 cause an autosomal recessive
            neurodevelopmental disorder
  JOURNAL   Genet Med 25 (9), 100900 (2023)
   PUBMED   37226891
  REMARK    GeneRIF: Biallelic variants in NSUN6 cause an autosomal recessive
            neurodevelopmental disorder.
REFERENCE   3  (bases 1 to 1930)
  AUTHORS   Awah,C.U., Winter,J., Mazdoom,C.M. and Ogunwobi,O.O.
  TITLE     NSUN6, an RNA methyltransferase of 5-mC controls glioblastoma
            response to temozolomide (TMZ) via NELFB and RPS6KB2 interaction
  JOURNAL   Cancer Biol Ther 22 (10-12), 587-597 (2021)
   PUBMED   34705606
  REMARK    GeneRIF: NSUN6, an RNA methyltransferase of 5-mC controls
            glioblastoma response to temozolomide (TMZ) via NELFB and RPS6KB2
            interaction.
REFERENCE   4  (bases 1 to 1930)
  AUTHORS   Selmi,T., Hussain,S., Dietmann,S., Heiss,M., Borland,K., Flad,S.,
            Carter,J.M., Dennison,R., Huang,Y.L., Kellner,S., Bornelov,S. and
            Frye,M.
  TITLE     Sequence- and structure-specific cytosine-5 mRNA methylation by
            NSUN6
  JOURNAL   Nucleic Acids Res 49 (2), 1006-1022 (2021)
   PUBMED   33330931
  REMARK    GeneRIF: Sequence- and structure-specific cytosine-5 mRNA
            methylation by NSUN6.
REFERENCE   5  (bases 1 to 1930)
  AUTHORS   Yang,R., Liang,X., Wang,H., Guo,M., Shen,H., Shi,Y., Liu,Q.,
            Sun,Y., Yang,L. and Zhan,M.
  TITLE     The RNA methyltransferase NSUN6 suppresses pancreatic cancer
            development by regulating cell proliferation
  JOURNAL   EBioMedicine 63, 103195 (2021)
   PUBMED   33418496
  REMARK    GeneRIF: The RNA methyltransferase NSUN6 suppresses pancreatic
            cancer development by regulating cell proliferation.
REFERENCE   6  (bases 1 to 1930)
  AUTHORS   Liu,R.J., Long,T., Li,J., Li,H. and Wang,E.D.
  TITLE     Structural basis for substrate binding and catalytic mechanism of a
            human RNA:m5C methyltransferase NSun6
  JOURNAL   Nucleic Acids Res 45 (11), 6684-6697 (2017)
   PUBMED   28531330
  REMARK    GeneRIF: The structures of NSun6, a human tRNA:m5C
            methyltransferase, in the apo form and in complex with a
            full-length tRNA substrate.
REFERENCE   7  (bases 1 to 1930)
  AUTHORS   Long,T., Li,J., Li,H., Zhou,M., Zhou,X.L., Liu,R.J. and Wang,E.D.
  TITLE     Sequence-specific and Shape-selective RNA Recognition by the Human
            RNA 5-Methylcytosine Methyltransferase NSun6
  JOURNAL   J Biol Chem 291 (46), 24293-24303 (2016)
   PUBMED   27703015
  REMARK    GeneRIF: findings suggest that human NSun6 relies on a delicate
            network for RNA recognition, which involves both the primary
            sequence and tertiary structure of tRNA substrates.
REFERENCE   8  (bases 1 to 1930)
  AUTHORS   Haag,S., Warda,A.S., Kretschmer,J., Gunnigmann,M.A., Hobartner,C.
            and Bohnsack,M.T.
  TITLE     NSUN6 is a human RNA methyltransferase that catalyzes formation of
            m5C72 in specific tRNAs
  JOURNAL   RNA 21 (9), 1532-1543 (2015)
   PUBMED   26160102
  REMARK    GeneRIF: Findings identified NSUN6 as a novel mammalian m5C tRNA
            methyltransferase and suggest that methylation of tRNAs by NSUN6
            occurs during a late step of tRNA biogenesis, after the addition of
            the CCA tail and export of the tRNA from the nucleus.
REFERENCE   9  (bases 1 to 1930)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            HY169016.1, BQ224447.1 and BC035778.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001351118.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.663306.1,
                                           SRR18074968.546365.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-331               HY169016.1         55-385
            332-577             BQ224447.1         362-607
            578-1622            BC035778.1         850-1894
            1623-1930           BC035778.1         1903-2210
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p12.31"
     gene            1..1930
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="NOP2/Sun RNA methyltransferase 6"
                     /db_xref="GeneID:221078"
                     /db_xref="HGNC:HGNC:23529"
                     /db_xref="MIM:617199"
     exon            1..459
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            460..569
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            570..723
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             572..1558
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="isoform 5 is encoded by transcript variant 5;
                     nucleolar protein (NOL1/NOP2/sun) and PUA domains 1;
                     NOL1/NOP2/Sun domain family, member 6; putative
                     methyltransferase NSUN6; ARL5B antisense RNA 1;
                     NOL1/NOP2/Sun and PUA domain-containing protein 1;
                     NOP2/Sun RNA methyltransferase family member 6; tRNA
                     (cytosine(72)-C(5))-methyltransferase NSUN6"
                     /codon_start=1
                     /product="tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
                     isoform 5"
                     /protein_id="NP_001338047.1"
                     /db_xref="GeneID:221078"
                     /db_xref="HGNC:HGNC:23529"
                     /db_xref="MIM:617199"
                     /translation="MKAGDVISVYSDIKGKCKKGAKEFDGTKVFLGNGISELSRKEIF
                     SGLPELKGMGIRMTEPVYLSPSFDSVLPRYLFLQNLPSALVSHVLNPQPGEKILDLCA
                     APGGKTTHIAALMHDQGEVIALDKIFNKVEKIKQNALLLGLNSIRAFCFDGTKAVKLD
                     MVEDTEGEPPFLPESFDRILLDAPCSGMGQRPNMACTWSVKEVASYQPLQRKLFTAAV
                     QLLKPEGVLVYSTCTITLAENEEQVAWALTKFPCLQLQPQEPQIGGEGMRGAGLSCEQ
                     LKQLQRFDPSAVPLPDTDMDSLREARREDMLRLANKDSIGFFIAKFVKCKST"
     exon            724..805
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            806..925
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1070
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1219
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1345
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1930
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1911..1916
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="hexamer: AATAAA"
     polyA_site      1930
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="major polyA site"
ORIGIN      
        1 gatagaaaag ataaagggac cgagctgctg tcagcctggc ttactgatct gcgtccgttt
       61 caccacggat tcagttacta agcatttttt tctttttttg gttctttgca acgtgagtgg
      121 cattggctca gtgatttcca tgagcatctc taccagaaaa cattgcctcg atgaggtgtg
      181 gtggaagccc ggcagcccct tctaatcggc taggcttgag aaagcgtgta cctctgcatt
      241 tccgaaatta actcagcgtg atcggcaaga ttttcctcag catctggtgt caagacactc
      301 gtcactatta attcggaaag aaaaaaaaaa acaaaacacc gttttccagc atttctcttg
      361 gtggagaact aaacaacagg aaaaatgtct attttcccta agatatcttt gagacctgag
      421 gttgaaaact atcttaagga aggctttatg aataaggaga aagaatatta aaaaacaaca
      481 gtgtgaagcc attgttggag cccagtgtgg caatgcagtt ttaagaggag cccatgtcta
      541 tgccccagga attgtgtcag catcacaatt tatgaaagct ggagatgtta tttctgtata
      601 ctctgatatt aaaggaaaat gtaagaaagg agccaaagaa tttgatggaa caaaagtatt
      661 tcttggaaat gggatttctg aactaagccg caaagaaatc ttcagtggat tacctgaact
      721 gaaaggcatg ggcataagaa tgacagaacc agtatatctc agcccttcat ttgacagtgt
      781 actgccccgt tacttatttt tacaaaattt gccatctgcc ttagtaagtc atgtactaaa
      841 tcctcaacct ggagagaaga ttctagactt gtgtgcagca cctggaggga aaacaacaca
      901 cattgcagca ctaatgcatg atcagggaga agttatagca ctggataaaa tcttcaacaa
      961 agtagaaaaa atcaaacaga atgccttatt gttagggctg aattccatca gggcattttg
     1021 ttttgatgga acaaaggcgg ttaaacttga tatggtggag gacacagaag gagaacctcc
     1081 atttctacca gaatcctttg accgaattct tctggatgca ccctgtagtg gaatgggaca
     1141 gagaccaaac atggcctgta cttggtctgt gaaggaagtg gcatcatatc agccattaca
     1201 gcgaaaactc ttcactgcag cggttcagct gctgaagcca gagggtgtgc tggtttatag
     1261 cacgtgcact ataacactgg ccgaaaatga agaacaggtt gcctgggccc tgacaaaatt
     1321 tccttgcctt cagcttcagc cccaggaacc gcagattgga ggagaaggaa tgaggggagc
     1381 tgggctctca tgtgaacagt tgaaacagct gcagcgattt gatccatcgg ctgtgccatt
     1441 accggacact gacatggact ctcttagaga ggccagaaga gaagacatgt tgcgtctggc
     1501 taataaggac tctataggtt tttttattgc aaaatttgta aaatgcaaaa gcacatagga
     1561 gagggatgga tgctcagaaa tgaaaattcc aaacatttgc tgtctgtggt tttttttttt
     1621 ttaaccaaag tgttgtcagg ccaactgaat gatgatgtgg ttgctatgga aacagaaaag
     1681 gctgccagct gttttaccag ggatccagag acatagagga agtagggggt ggtatgagat
     1741 tatattttct gtttttaaaa gatttttttt ttttatgtat ttagtagagt ataaagaaaa
     1801 gcagatgcct atagatgtct ggagcatatt ttcatttgtg atctaatgtt ttaatttgta
     1861 aagtgtacaa gtcattttta atgttaaaaa ttagtgaatc taacaaaagg aataaattag
     1921 caatatttga
//
