LOCUS       NM_001351117            2010 bp    mRNA    linear   PRI 12-APR-2024
DEFINITION  Homo sapiens NOP2/Sun RNA methyltransferase 6 (NSUN6), transcript
            variant 4, mRNA.
ACCESSION   NM_001351117 XM_017015909
VERSION     NM_001351117.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Lu,Z., Liu,B., Kong,D., Zhou,X., Pei,D. and Liu,D.
  TITLE     NSUN6 Regulates NM23-H1 Expression in an m5C Manner to Affect
            Epithelial-Mesenchymal Transition in Lung Cancer
  JOURNAL   Med Princ Pract 33 (1), 56-65 (2024)
   PUBMED   38029727
  REMARK    GeneRIF: NSUN6 Regulates NM23-H1 Expression in an m5C Manner to
            Affect Epithelial-Mesenchymal Transition in Lung Cancer.
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Mattioli,F., Worpenberg,L., Li,C.T., Ibrahim,N., Naz,S., Sharif,S.,
            Firouzabadi,S.G., Vosoogh,S., Saraeva-Lamri,R., Raymond,L.,
            Trujillo,C., Guex,N., Antonarakis,S.E., Ansar,M., Darvish,H.,
            Liu,R.J., Roignant,J.Y. and Reymond,A.
  TITLE     Biallelic variants in NSUN6 cause an autosomal recessive
            neurodevelopmental disorder
  JOURNAL   Genet Med 25 (9), 100900 (2023)
   PUBMED   37226891
  REMARK    GeneRIF: Biallelic variants in NSUN6 cause an autosomal recessive
            neurodevelopmental disorder.
REFERENCE   3  (bases 1 to 2010)
  AUTHORS   Awah,C.U., Winter,J., Mazdoom,C.M. and Ogunwobi,O.O.
  TITLE     NSUN6, an RNA methyltransferase of 5-mC controls glioblastoma
            response to temozolomide (TMZ) via NELFB and RPS6KB2 interaction
  JOURNAL   Cancer Biol Ther 22 (10-12), 587-597 (2021)
   PUBMED   34705606
  REMARK    GeneRIF: NSUN6, an RNA methyltransferase of 5-mC controls
            glioblastoma response to temozolomide (TMZ) via NELFB and RPS6KB2
            interaction.
REFERENCE   4  (bases 1 to 2010)
  AUTHORS   Selmi,T., Hussain,S., Dietmann,S., Heiss,M., Borland,K., Flad,S.,
            Carter,J.M., Dennison,R., Huang,Y.L., Kellner,S., Bornelov,S. and
            Frye,M.
  TITLE     Sequence- and structure-specific cytosine-5 mRNA methylation by
            NSUN6
  JOURNAL   Nucleic Acids Res 49 (2), 1006-1022 (2021)
   PUBMED   33330931
  REMARK    GeneRIF: Sequence- and structure-specific cytosine-5 mRNA
            methylation by NSUN6.
REFERENCE   5  (bases 1 to 2010)
  AUTHORS   Yang,R., Liang,X., Wang,H., Guo,M., Shen,H., Shi,Y., Liu,Q.,
            Sun,Y., Yang,L. and Zhan,M.
  TITLE     The RNA methyltransferase NSUN6 suppresses pancreatic cancer
            development by regulating cell proliferation
  JOURNAL   EBioMedicine 63, 103195 (2021)
   PUBMED   33418496
  REMARK    GeneRIF: The RNA methyltransferase NSUN6 suppresses pancreatic
            cancer development by regulating cell proliferation.
REFERENCE   6  (bases 1 to 2010)
  AUTHORS   Liu,R.J., Long,T., Li,J., Li,H. and Wang,E.D.
  TITLE     Structural basis for substrate binding and catalytic mechanism of a
            human RNA:m5C methyltransferase NSun6
  JOURNAL   Nucleic Acids Res 45 (11), 6684-6697 (2017)
   PUBMED   28531330
  REMARK    GeneRIF: The structures of NSun6, a human tRNA:m5C
            methyltransferase, in the apo form and in complex with a
            full-length tRNA substrate.
REFERENCE   7  (bases 1 to 2010)
  AUTHORS   Long,T., Li,J., Li,H., Zhou,M., Zhou,X.L., Liu,R.J. and Wang,E.D.
  TITLE     Sequence-specific and Shape-selective RNA Recognition by the Human
            RNA 5-Methylcytosine Methyltransferase NSun6
  JOURNAL   J Biol Chem 291 (46), 24293-24303 (2016)
   PUBMED   27703015
  REMARK    GeneRIF: findings suggest that human NSun6 relies on a delicate
            network for RNA recognition, which involves both the primary
            sequence and tertiary structure of tRNA substrates.
REFERENCE   8  (bases 1 to 2010)
  AUTHORS   Haag,S., Warda,A.S., Kretschmer,J., Gunnigmann,M.A., Hobartner,C.
            and Bohnsack,M.T.
  TITLE     NSUN6 is a human RNA methyltransferase that catalyzes formation of
            m5C72 in specific tRNAs
  JOURNAL   RNA 21 (9), 1532-1543 (2015)
   PUBMED   26160102
  REMARK    GeneRIF: Findings identified NSUN6 as a novel mammalian m5C tRNA
            methyltransferase and suggest that methylation of tRNAs by NSUN6
            occurs during a late step of tRNA biogenesis, after the addition of
            the CCA tail and export of the tRNA from the nucleus.
REFERENCE   9  (bases 1 to 2010)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            HY169016.1, DB012566.1 and BC035778.1.
            
            On May 31, 2019 this sequence version replaced NM_001351117.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.125323.1,
                                           SRR1803611.222136.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-331               HY169016.1         55-385
            332-360             DB012566.1         361-389
            361-459             BC035778.1         397-495
            460-1702            BC035778.1         652-1894
            1703-2010           BC035778.1         1903-2210
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p12.31"
     gene            1..2010
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="NOP2/Sun RNA methyltransferase 6"
                     /db_xref="GeneID:221078"
                     /db_xref="HGNC:HGNC:23529"
                     /db_xref="MIM:617199"
     exon            1..459
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             385..1638
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="isoform 4 is encoded by transcript variant 4;
                     nucleolar protein (NOL1/NOP2/sun) and PUA domains 1;
                     NOL1/NOP2/Sun domain family, member 6; putative
                     methyltransferase NSUN6; ARL5B antisense RNA 1;
                     NOL1/NOP2/Sun and PUA domain-containing protein 1;
                     NOP2/Sun RNA methyltransferase family member 6; tRNA
                     (cytosine(72)-C(5))-methyltransferase NSUN6"
                     /codon_start=1
                     /product="tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
                     isoform 4"
                     /protein_id="NP_001338046.1"
                     /db_xref="GeneID:221078"
                     /db_xref="HGNC:HGNC:23529"
                     /db_xref="MIM:617199"
                     /translation="MSIFPKISLRPEVENYLKEGFMNKEQFNGLSVPILQHPDLQDVL
                     LIPVIGPRKNIKKQQCEAIVGAQCGNAVLRGAHVYAPGIVSASQFMKAGDVISVYSDI
                     KGKCKKGAKEFDGTKVFLGNGISELSRKEIFSGLPELKGMGIRMTEPVYLSPSFDSVL
                     PRYLFLQNLPSALVSHVLNPQPGEKILDLCAAPGGKTTHIAALMHDQGEVIALDKIFN
                     KVEKIKQNALLLGLNSIRAFCFDGTKAVKLDMVEDTEGEPPFLPESFDRILLDAPCSG
                     MGQRPNMACTWSVKEVASYQPLQRKLFTAAVQLLKPEGVLVYSTCTITLAENEEQVAW
                     ALTKFPCLQLQPQEPQIGGEGMRGAGLSCEQLKQLQRFDPSAVPLPDTDMDSLREARR
                     EDMLRLANKDSIGFFIAKFVKCKST"
     exon            460..539
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            540..649
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            650..803
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            804..885
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            886..1005
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1150
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1299
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1425
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..2010
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1991..1996
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="hexamer: AATAAA"
     polyA_site      2010
                     /gene="NSUN6"
                     /gene_synonym="4933414E04Rik; ARL5B-AS1; MRT82; NOPD1"
                     /note="major polyA site"
ORIGIN      
        1 gatagaaaag ataaagggac cgagctgctg tcagcctggc ttactgatct gcgtccgttt
       61 caccacggat tcagttacta agcatttttt tctttttttg gttctttgca acgtgagtgg
      121 cattggctca gtgatttcca tgagcatctc taccagaaaa cattgcctcg atgaggtgtg
      181 gtggaagccc ggcagcccct tctaatcggc taggcttgag aaagcgtgta cctctgcatt
      241 tccgaaatta actcagcgtg atcggcaaga ttttcctcag catctggtgt caagacactc
      301 gtcactatta attcggaaag aaaaaaaaaa acaaaacacc gttttccagc atttctcttg
      361 gtggagaact aaacaacagg aaaaatgtct attttcccta agatatcttt gagacctgag
      421 gttgaaaact atcttaagga aggctttatg aataaggagc agtttaatgg attaagtgtt
      481 cctattcttc aacatccaga ccttcaagat gtgttactta ttcctgttat tggacccaga
      541 aagaatatta aaaaacaaca gtgtgaagcc attgttggag cccagtgtgg caatgcagtt
      601 ttaagaggag cccatgtcta tgccccagga attgtgtcag catcacaatt tatgaaagct
      661 ggagatgtta tttctgtata ctctgatatt aaaggaaaat gtaagaaagg agccaaagaa
      721 tttgatggaa caaaagtatt tcttggaaat gggatttctg aactaagccg caaagaaatc
      781 ttcagtggat tacctgaact gaaaggcatg ggcataagaa tgacagaacc agtatatctc
      841 agcccttcat ttgacagtgt actgccccgt tacttatttt tacaaaattt gccatctgcc
      901 ttagtaagtc atgtactaaa tcctcaacct ggagagaaga ttctagactt gtgtgcagca
      961 cctggaggga aaacaacaca cattgcagca ctaatgcatg atcagggaga agttatagca
     1021 ctggataaaa tcttcaacaa agtagaaaaa atcaaacaga atgccttatt gttagggctg
     1081 aattccatca gggcattttg ttttgatgga acaaaggcgg ttaaacttga tatggtggag
     1141 gacacagaag gagaacctcc atttctacca gaatcctttg accgaattct tctggatgca
     1201 ccctgtagtg gaatgggaca gagaccaaac atggcctgta cttggtctgt gaaggaagtg
     1261 gcatcatatc agccattaca gcgaaaactc ttcactgcag cggttcagct gctgaagcca
     1321 gagggtgtgc tggtttatag cacgtgcact ataacactgg ccgaaaatga agaacaggtt
     1381 gcctgggccc tgacaaaatt tccttgcctt cagcttcagc cccaggaacc gcagattgga
     1441 ggagaaggaa tgaggggagc tgggctctca tgtgaacagt tgaaacagct gcagcgattt
     1501 gatccatcgg ctgtgccatt accggacact gacatggact ctcttagaga ggccagaaga
     1561 gaagacatgt tgcgtctggc taataaggac tctataggtt tttttattgc aaaatttgta
     1621 aaatgcaaaa gcacatagga gagggatgga tgctcagaaa tgaaaattcc aaacatttgc
     1681 tgtctgtggt tttttttttt ttaaccaaag tgttgtcagg ccaactgaat gatgatgtgg
     1741 ttgctatgga aacagaaaag gctgccagct gttttaccag ggatccagag acatagagga
     1801 agtagggggt ggtatgagat tatattttct gtttttaaaa gatttttttt ttttatgtat
     1861 ttagtagagt ataaagaaaa gcagatgcct atagatgtct ggagcatatt ttcatttgtg
     1921 atctaatgtt ttaatttgta aagtgtacaa gtcattttta atgttaaaaa ttagtgaatc
     1981 taacaaaagg aataaattag caatatttga
//
